ViewGene
Locus Name:
GeneID:
Location:

GeneID:1241
TrivialName: PA14_57780
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_57780
PAO1 Ortholog Locus:PA4448
Sequence Length:1323
Protein Length:440
Start: 5147017
Stop: 5145695
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07836.1|AE004859_5 (AE004859) histidinol dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 440/440 (100%), Positives = 440/440 (100%)
Sequence:
ATGACCGCTCCCTTCGCTATCCGTCGACTCAATGCCGCTGATCCGGACTTCGGGCGTCATCTGGATCATCTG
CTGAGCTGGGAAAGTGTCTCCGACGATTCGGTCAACCAGCGCGTCCTCGACATCATCGCCGCCGTACGCAGC
CGTGGCGACGCCGCCGTGGTCGAGTTCACCCAGCGCTTCGACGGTCTCCAGGCCGCCTCGATGGCTGACCTG
ATCCTGCCGCGCGAGCGCCTGGAGCTGGCTCTCACCCGTATCACCGTAGCCCAGCGCGAAGCCCTGGAAGTC
GCCGCCGAGCGCGTGCGCAGCTACCACGAGAAGCAGAAGCAGGGTTCCTGGCGCTACACCGAGGCCGACGGC
ACGGTGCTCGGCCAGCAGGTCACTCCCCTGGATCGCGCCGGGCTCTATGTGCCGGGCGGCAAGGCGTCCTAC
CCGTCCTCGGTGCTGATGAACGCGATCCCGGCCAAGGTCGCCGGGGTGTCCGAAGTGGTCATGGTCGTACCG
ACCCCGCGCGGCGAGATCAACGAGATCGTCCTCGCCGCCGCCTGCATCGCCGGTGTCGACCGTGTCTTCACC
ATCGGCGGGGCACAGGCCGTGGCCGCGTTGGCCTACGGCACCGAAAGCGTGCCGCGGGTGGACAAGATCGTC
GGCCCCGGCAACATCTACGTGGCCACCGCCAAGCGTCACGTGTTCGGCCAGGTCGGCATCGACATGATCGCC
GGTCCCTCGGAAATCCTCGTGGTCTGCGATGGCCAGACCGATCCCGACTGGATCGCCATGGACCTGTTCTCC
CAGGCCGAACACGACGAAGACGCCCAGTCGATCCTGGTCAGTCCGGACGCGGCGTTCCTGGACCGCGTCGCG
GACAGCATCGCTCGCCTGCTGCCGACCATGGAGCGCGCCGAGATCATCCGCACCTCCCTGGAGGGGCGTGGC
GCGCTGATCCAGGTCGCCGACCAGGCCCAGGCCTGCGCGGTGGCCAACCGCATCGCCCCGGAACACCTGGAA
CTGTCGGTGGCCGATCCGGAAAGCTGGCTGCCGGAGATCCGCCATGCCGGTGCGATCTTCATGGGCCGCTAC
ACTGCCGAGGCCCTGGGCGACTACTGCGCCGGCCCGAACCACGTGCTGCCGACCTCCGGTACCGCGCGTTTC
TCTTCGCCGCTGGGGGTATATGACTTCCAGAAGCGTTCGTCGATCATCAACTGCTCGGCCGAGGGCGCTTCG
GTCCTCGGTCGCACCGCTTCGGTGCTGGCTCGCGGCGAGTCGTTGACCGCCCACGCGCGCAGCGCCGAATAC
CGCATCCTCGACGAGAAGGAGGCCTGA
Translation:
MTAPFAIRRLNAADPDFGRHLDHLLSWESVSDDSVNQRVLDIIAAVRSRGDAAVVEFTQRFDGLQAASMADL
ILPRERLELALTRITVAQREALEVAAERVRSYHEKQKQGSWRYTEADGTVLGQQVTPLDRAGLYVPGGKASY
PSSVLMNAIPAKVAGVSEVVMVVPTPRGEINEIVLAAACIAGVDRVFTIGGAQAVAALAYGTESVPRVDKIV
GPGNIYVATAKRHVFGQVGIDMIAGPSEILVVCDGQTDPDWIAMDLFSQAEHDEDAQSILVSPDAAFLDRVA
DSIARLLPTMERAEIIRTSLEGRGALIQVADQAQACAVANRIAPEHLELSVADPESWLPEIRHAGAIFMGRY
TAEALGDYCAGPNHVLPTSGTARFSSPLGVYDFQKRSSIINCSAEGASVLGRTASVLARGESLTAHARSAEY
RILDEKEA*
AnnotationID:1250GeneID:1241
AnnotatorUID: diggins
Modification Date/Time: 2005-05-03 06:06:23
Gene Name:hisD
Confidence Code:2
GeneProduct: histidinol dehydrogenase
Cell Localization:()
Synonyms:hisX
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0141,pfam00815,KOG2697
Secondary Functional Category(ies):
EC Number:1.1.1.23
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57887.1| (AE016872) histidinol dehydrogenase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 448

 Score =  765 bits (1975), Expect = 0.0
 Identities = 388/436 (88%), Positives = 408/436 (93%)

>gb|AAN66591.1|AE016287_2 (AE016777) histidinol dehydrogenase [Pseudomonas putida KT2440]
          Length = 441

 Score =  748 bits (1931), Expect = 0.0
 Identities = 380/439 (86%), Positives = 405/439 (92%)






 
Structural Features:
COG0141, HisD, Histidinol dehydrogenase 
[Amino acid transport and metabolism]
CD-Length = 425 residues, 100.0% aligned
Score =  548 bits (1413), Expect = 1e-156
Identities = 225/433 (51%), Positives = 288/433 (66%), Gaps = 9/433 (2%)

pfam00815, Histidinol_dh, Histidinol dehydrogenase. 
CD-Length = 413 residues,  99.5% aligned
Score =  447 bits (1152), Expect = 2e-126
Identities = 193/415 (46%), Positives = 260/415 (62%), Gaps = 6/415 (1%)

KOG2697, Histidinol dehydrogenase 
[Amino acid transport and metabolism]
CD-Length = 446 residues,  93.7% aligned
Score =  297 bits (762), Expect = 3e-81
Identities = 158/424 (37%), Positives = 232/424 (54%), Gaps = 15/424 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2485




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19628




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31486




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1241_cdd.html


    		




 
URL:

















    ReferenceID:32837




  
Author/Investigator(s):
        Carlomagno MS, Chiariotti L, Alifano P, Nappo AG, Bruni CB.





  
Title:
        Structure and function of the Salmonella typhimurium and Escherichia coli K-12 histidine operons.




  
PubMed:
        3062174


MedLine:
        




    
Source:
J Mol Biol. 1988 Oct 5;203(3):585-606.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3062174

















    ReferenceID:32838




  
Author/Investigator(s):
        Chiariotti L, Alifano P, Carlomagno MS, Bruni CB.





  
Title:
        Nucleotide sequence of the Escherichia coli hisD gene and of the Escherichia coli and Salmonella typhimurium hisIE region.




  
PubMed:
        3018428


MedLine:
        




    
Source:
Mol Gen Genet. 1986 Jun;203(3):382-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3018428

















    ReferenceID:32839




  
Author/Investigator(s):
        Riggs D, Artz S.





  
Title:
        The hisD-hisC gene border of the Salmonella typhimurium histidine operon.




  
PubMed:
        6390096


MedLine:
        




    
Source:
Mol Gen Genet. 1984;196(3):526-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6390096

















    ReferenceID:32840




  
Author/Investigator(s):
        Ino I, Hartman PE, Hartman Z, Yourno J.





  
Title:
        Deletions fusing the hisG and hisD genes in Salmonella typhimurium.




  
PubMed:
        1099075


MedLine:
        




    
Source:
J Bacteriol. 1975 Sep;123(3):1254-64.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1099075









           
Homologs By Global Alignment 

           

       



     
Gene ID:1241
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2258
                           PA4448_tr
                           translation of PA4448
                           440
                           99.99
                           99.99
                           0
                           2214.0
     

     
     
73921
                           gb|AAG07836.1|AE004859_5
                           (AE004859) histidinol dehydrogenase [Pseudomonas aeruginosa PAO1]
                           440
                           99.99
                           99.99
                           0
                           2214.0
     

     
     
73922
                           gb|AAO57887.1|
                           (AE016872) histidinol dehydrogenase [Pseudomonas syringae pv. tomato str. DC3000]
                           448
                           87.05
                           91.51
                           8
                           1970.0
     

     
     
73923
                           gb|AAN66591.1|AE016287_2
                           (AE016777) histidinol dehydrogenase [Pseudomonas putida KT2440]
                           441
                           86.16
                           91.83
                           1
                           1931.0
     

     
     
73924
                           gb|AAQ58287.1|
                           (AE016912) histidinol dehydrogenase [Chromobacterium violaceum ATCC 12472]
                           440
                           67.27
                           79.54
                           6
                           1512.0
     

     






      

          
GC ORFID: 44243How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4448Glimmer Score: 

          
Start: 4410696Stop: 4409374

          
Length: 1323


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4448 translation of PA4448Homolog Bit Score 857.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5527 (ORF: BLASTX 772.0), t_PA4448 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCGCTCCCTTCGCTATCCGTCGACTCAATGCCGCTGATCCGGACTTCGGGCGTCATCTGGATCATCTG 
CTGAGCTGGGAAAGTGTCTCCGACGATTCGGTCAACCAGCGCGTCCTCGACATCATCGCCGCCGTACGCAGC 
CGTGGCGACGCCGCCGTGGTCGAGTTCACCCAGCGCTTCGACGGTCTCCAGGCCGCCTCGATGGCTGACCTG 
ATCCTGCCGCGCGAGCGCCTGGAGCTGGCTCTCACCCGTATCACCGTAGCCCAGCGCGAAGCCCTGGAAGTC 
GCCGCCGAGCGCGTGCGCAGCTACCACGAGAAGCAGAAGCAGGGTTCCTGGCGCTACACCGAGGCCGACGGC 
ACGGTGCTCGGCCAGCAGGTCACTCCCCTGGATCGCGCCGGGCTCTATGTGCCGGGCGGCAAGGCGTCCTAC 
CCGTCCTCGGTGCTGATGAACGCGATCCCGGCCAAGGTCGCCGGGGTGTCCGAAGTGGTCATGGTCGTACCG 
ACCCCGCGCGGCGAGATCAACGAGATCGTCCTCGCCGCCGCCTGCATCGCCGGTGTCGACCGTGTCTTCACC 
ATCGGCGGGGCACAGGCCGTGGCCGCGTTGGCCTACGGCACCGAAAGCGTGCCGCGGGTGGACAAGATCGTC 
GGCCCCGGCAACATCTACGTGGCCACCGCCAAGCGTCACGTGTTCGGCCAGGTCGGCATCGACATGATCGCC 
GGTCCCTCGGAAATCCTCGTGGTCTGCGATGGCCAGACCGATCCCGACTGGATCGCCATGGACCTGTTCTCC 
CAGGCCGAACACGACGAAGACGCCCAGTCGATCCTGGTCAGTCCGGACGCGGCGTTCCTGGACCGCGTCGCG 
GACAGCATCGCTCGCCTGCTGCCGACCATGGAGCGCGCCGAGATCATCCGCACCTCCCTGGAGGGGCGTGGC 
GCGCTGATCCAGGTCGCCGACCAGGCCCAGGCCTGCGCGGTGGCCAACCGCATCGCCCCGGAACACCTGGAA 
CTGTCGGTGGCCGATCCGGAAAGCTGGCTGCCGGAGATCCGCCATGCCGGTGCGATCTTCATGGGCCGCTAC 
ACTGCCGAGGCCCTGGGCGACTACTGCGCCGGCCCGAACCACGTGCTGCCGACCTCCGGTACCGCGCGTTTC 
TCTTCGCCGCTGGGGGTATATGACTTCCAGAAGCGTTCGTCGATCATCAACTGCTCGGCCGAGGGCGCTTCG 
GTCCTCGGTCGCACCGCTTCGGTGCTGGCTCGCGGCGAGTCGTTGACCGCCCACGCGCGCAGCGCCGAATAC 
CGCATCCTCGACGAGAAGGAGGCCTGA