ViewGene
Locus Name:
GeneID:
Location:

GeneID:1233
TrivialName: PA14_14890
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_14890
PAO1 Ortholog Locus:PA3802
Sequence Length:1290
Protein Length:429
Start: 1264132
Stop: 1265421
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07189.1|AE004798_12 (AE004798) histidyl-tRNA synthetase [Pseudomonas aeruginosa PAO1]
Identities = 429/429 (100%), Positives = 429/429 (100%)
Sequence:
GTGAGCAAGTCCCTGCAAGCCATTCGTGGCATGAACGACATCCTGCCGGAGCAGACTCCGGCCTGGCGCTAT
CTGGAACGCACCTTCGCCGGCCTGCTGGACGGCTACGGCTACAGCGAGATCCGCCTGCCCATCCTCGAGTTC
ACCGAACTCTTCGCCCGCGGCATCGGCGAAGGTACCGACGTGGTCGACAAGGAGATGTACACCTTCCTCGAC
CGCAACGGCGAATCCCTGACCATGCGTCCGGAAGGTACCGCCGGTTGCGTGCGCGCCGTGCTGGAGCACGGC
CTGAGCGGTGGTGGCCAGGTGCAGAAGCTCTGGTACACCGGGCCGATGTTCCGTTACGAGAAACCGCAGAAA
GGTCGCTACCGGCAGTTCCACCAGATCGGCGTGGAAGTCTTCAACCTGCCCGGTCCGGACATCGACGCCGAG
TTGATCATCCTCACCTGGCGCCTGTGGCAGAAGCTCGGCATGGCCGACGCGGTGACCCTGCAGCTCAACACC
CTCGGTTCCAGCGAGGCCCGCGCACGCTATCGCGAGGCCCTGGTGGCCTATCTGCAGGAGCGCTTCGAGCAA
CTCGACGAAGACAGCCAGCGGCGCATGACCACCAATCCGCTGCGAATCCTCGACAGCAAGGTGGAGAGCACC
CAGGCGTTGCTGGTCGGCGCGCCGACCCTGCACGACTATCTCGACGAAGAATCGATCGCCCACTTCGAGGGT
CTCAAGGCCCGCCTGGATGCTGTCGGACTGCGCTACGAAATCAACCAGAAGCTGGTGCGCGGCCTGGACTAT
TACTGCCGCACGGCTTTCGAGTGGGTCACCGACAAGCTTGGCGCCCAGGGCACCGTTTGCGGCGGTGGCCGC
TACGACGGGCTGGTCAGCCAGTTCGGCGGCAAGCCGACCCCGGGCGTCGGTTTCGCCATGGGCGTGGAACGC
CTGGTGCTGCTGCTGGAAACCTTGGGGGTGATCCCCGCCGAACTGAACCGTCCGGCCGACCTCTACGTCTGC
GCCTTCGGCGAACCGGCCGAGCTGGCCGCCCTGACCCTGGCCGAACAGTTGCGCAGCGCCATTCCCGGCATA
CGCTTGCTGGTCAACGCCGGCGCCGGTAGCTTCAAGAGCCAGTTCAAGAAGGCCGACAAGAGCGGCGCGCGC
TTTGCCCTGATCCTCGGGGAGGACGAGGTGGCCAATCGCGTGGTAGGTTTCAAGCCCCTGCGTGACGAGGGC
GAGCAACAAAGCATTGCCTGGGATGCTCTGCCCGAGCACCTGGCAGCCTGTCTGGCACAGGCTTGA
Translation:
MSKSLQAIRGMNDILPEQTPAWRYLERTFAGLLDGYGYSEIRLPILEFTELFARGIGEGTDVVDKEMYTFLD
RNGESLTMRPEGTAGCVRAVLEHGLSGGGQVQKLWYTGPMFRYEKPQKGRYRQFHQIGVEVFNLPGPDIDAE
LIILTWRLWQKLGMADAVTLQLNTLGSSEARARYREALVAYLQERFEQLDEDSQRRMTTNPLRILDSKVEST
QALLVGAPTLHDYLDEESIAHFEGLKARLDAVGLRYEINQKLVRGLDYYCRTAFEWVTDKLGAQGTVCGGGR
YDGLVSQFGGKPTPGVGFAMGVERLVLLLETLGVIPAELNRPADLYVCAFGEPAELAALTLAEQLRSAIPGI
RLLVNAGAGSFKSQFKKADKSGARFALILGEDEVANRVVGFKPLRDEGEQQSIAWDALPEHLAACLAQA*
AnnotationID:1242GeneID:1233
AnnotatorUID: diggins
Modification Date/Time: 2005-04-24 11:11:31
Gene Name:hisS
Confidence Code:2
GeneProduct: histidyl-tRNA synthetase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0124,KOG1936
Secondary Functional Category(ies):
EC Number:6.1.1.21
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66479.1|AE016276_14 (AE016777) histidyl-tRNA synthetase [Pseudomonas putida KT2440]
          Length = 429

 Score =  666 bits (1719), Expect = 0.0
 Identities = 325/429 (75%), Positives = 368/429 (85%)

>gb|AAC03121.1| (AF047040) histidyl-tRNA synthetase; aminoacyl-tRNA synthetase
           [Salmonella typhimurium]
          Length = 424

 Score =  516 bits (1329), Expect = e-145
 Identities = 265/427 (62%), Positives = 324/427 (75%), Gaps = 5/427 (1%)

>gb|AAC75567.1| (AE000337) histidine tRNA synthetase [Escherichia coli K12]
          Length = 424

 Score =  513 bits (1320), Expect = e-144
 Identities = 260/426 (61%), Positives = 322/426 (75%), Gaps = 3/426 (0%)






 
Structural Features:
COG0124, HisS, Histidyl-tRNA synthetase 
[Translation, ribosomal structure and biogenesis]
CD-Length = 429 residues,  99.8% aligned
Score =  503 bits (1296), Expect = 5e-143
Identities = 236/434 (54%), Positives = 284/434 (65%), Gaps = 15/434 (3%)

KOG1936, Histidyl-tRNA synthetase 
[Translation, ribosomal structure and biogenesis]
CD-Length = 518 residues,  85.3% aligned
Score =  124 bits (312), Expect = 4e-29
Identities = 113/452 (25%), Positives = 183/452 (40%), Gaps = 55/452 (12%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14309




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19612




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31755




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1233_cdd.html


    		




 
URL:

















    ReferenceID:34277




  
Author/Investigator(s):
        Menguito CA, Keherly MJ, Tang C, Papaconstantinou J, Weigel PH.





  
Title:
        Molecular cloning, sequence, structural analysis and expression of the histidyl-tRNA synthetase gene from Streptococcus equisimilis.




  
PubMed:
        8441673


MedLine:
        




    
Source:
Nucleic Acids Res. 1993 Feb 11;21(3):615-20.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8441673

















    ReferenceID:34278




  
Author/Investigator(s):
        Freedman R, Gibson B, Donovan D, Biemann K, Eisenbeis S, Parker J, Schimmel P.





  
Title:
        Primary structure of histidine-tRNA synthetase and characterization of hisS transcripts.




  
PubMed:
        2991272


MedLine:
        




    
Source:
J Biol Chem. 1985 Aug 25;260(18):10063-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2991272









           
Homologs By Global Alignment 

           

       



     
Gene ID:1233
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2247
                           PA3802_tr
                           translation of PA3802
                           429
                           99.99
                           99.99
                           0
                           2217.0
     

     
     
73605
                           gb|AAG07189.1|AE004798_12
                           (AE004798) histidyl-tRNA synthetase [Pseudomonas aeruginosa PAO1]
                           429
                           99.76
                           99.99
                           0
                           2214.0
     

     
     
73606
                           gb|AAN66479.1|AE016276_14
                           (AE016777) histidyl-tRNA synthetase [Pseudomonas putida KT2440]
                           429
                           75.52
                           85.78
                           0
                           1715.0
     

     
     
73607
                           gb|AAO54956.1|
                           (AE016860) histidyl-tRNA synthetase [Pseudomonas syringae pv. tomato str. DC3000]
                           429
                           73.42
                           85.54
                           0
                           1692.0
     

     
     
73609
                           gb|AAC03121.1|
                           (AF047040) histidyl-tRNA synthetase; aminoacyl-tRNA synthetase [Salmonella typhimurium]
                           430
                           61.39
                           75.34
                           7
                           1333.0
     

     






      

          
GC ORFID: 44235How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3802Glimmer Score: 

          
Start: 3689806Stop: 3688517

          
Length: 1290


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3802 translation of PA3802Homolog Bit Score 858.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4529 (ORF: BLASTX 664.0), t_PA3802 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGAGCAAGTCCCTGCAAGCCATTCGTGGCATGAACGACATCCTGCCGGAGCAGACTCCGGCCTGGCGCTAT 
CTGGAACGCACCTTCGCCGGCCTGCTGGACGGCTACGGCTACAGCGAGATCCGCCTGCCCATCCTCGAGTTC 
ACCGAACTCTTCGCCCGCGGCATCGGCGAAGGTACCGACGTGGTCGACAAGGAGATGTACACCTTCCTCGAC 
CGCAACGGCGAATCCCTGACCATGCGTCCGGAAGGTACCGCCGGTTGCGTGCGCGCCGTGCTGGAGCACGGC 
CTGAGCGGTGGTGGCCAGGTGCAGAAGCTCTGGTACACCGGGCCGATGTTCCGTTACGAGAAACCGCAGAAA 
GGTCGCTACCGGCAGTTCCACCAGATCGGCGTGGAAGTCTTCAACCTGCCCGGTCCGGACATCGACGCCGAG 
TTGATCATCCTCACCTGGCGCCTGTGGCAGAAGCTCGGCATGGCCGACGCGGTGACCCTGCAGCTCAACACC 
CTCGGTTCCAGCGAGGCCCGCGCACGCTATCGCGAGGCCCTGGTGGCCTATCTGCAGGAGCGCTTCGAGCAA 
CTCGACGAAGACAGCCAGCGGCGCATGACCACCAATCCGCTGCGAATCCTCGACAGCAAGGTGGAGAGCACC 
CAGGCGTTGCTGGTCGGCGCGCCGACCCTGCACGACTATCTCGACGAAGAATCGATCGCCCACTTCGAGGGT 
CTCAAGGCCCGCCTGGATGCTGTCGGACTGCGCTACGAAATCAACCAGAAGCTGGTGCGCGGCCTGGACTAT 
TACTGCCGCACGGCTTTCGAGTGGGTCACCGACAAGCTTGGCGCCCAGGGCACCGTTTGCGGCGGTGGCCGC 
TACGACGGGCTGGTCAGCCAGTTCGGCGGCAAGCCGACCCCGGGCGTCGGTTTCGCCATGGGCGTGGAACGC 
CTGGTGCTGCTGCTGGAAACCTTGGGGGTGATCCCCGCCGAACTGAACCGTCCGGCCGACCTCTACGTCTGC 
GCCTTCGGCGAACCGGCCGAGCTGGCCGCCCTGACCCTGGCCGAACAGTTGCGCAGCGCCATTCCCGGCATA 
CGCTTGCTGGTCAACGCCGGCGCCGGTAGCTTCAAGAGCCAGTTCAAGAAGGCCGACAAGAGCGGCGCGCGC 
TTTGCCCTGATCCTCGGGGAGGACGAGGTGGCCAATCGCGTGGTAGGTTTCAAGCCCCTGCGTGACGAGGGC 
GAGCAACAAAGCATTGCCTGGGATGCTCTGCCCGAGCACCTGGCAGCCTGTCTGGCACAGGCTTGA