ViewGene
Locus Name:
GeneID:
Location:

GeneID:1218
TrivialName: PA14_66790
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66790
PAO1 Ortholog Locus:PA5054
Sequence Length:1344
Protein Length:447
Start: 5964522
Stop: 5965865
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08439.1|AE004918_9 (AE004918) heat shock protein HslU [Pseudomonas aeruginosa PAO1]
Identities = 447/447 (100%), Positives = 447/447 (100%)
Sequence:
ATGTCCATGACGCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGC
GCCGTCGCCATCGCCCTGCGCAACCGCTGGCGACGCATGCAACTGCCGGCCGAGCTGCGCGCCGAGGTCACG
CCGAAGAACATCCTCATGATCGGCCCCACCGGCGTCGGCAAGACCGAGATCGCCCGCCGCCTGGCGCGCCTG
GCGAACGCGCCGTTCATCAAGGTCGAGGCGACCAAGTTCACCGAGGTCGGCTATGTCGGACGCGACGTCGAG
TCGATCATCCGCGATCTCGCCGACGCCGCGGTGAAGATGCTCCGCGAACAGGAGATCCAGAAGGTCAAGTAT
CGCGCCGAGGACGCCGCCGAAGAGCGCATCCTCGATGCCCTGCTGCCGGCCGCGCGTCCCGCCATGGGCTTC
GGCGACGAGCCGGCACGCGAGGACAGCAACACCCGCCAGCTGTTCCGCAAGCGCCTGCGCGAAGGCCAGCTG
GACGACAAGGAAATCGACATCGAGGTGGCCGACAACCCGGCCGGCGTGGAGATCATGGCCCCGCCCGGCATG
GAGGAGATGACCAACCAGTTGCAGAACCTGTTCTCCGGCATGAGCAAGGGCAAGAAGAAGACCCGCAAGCTG
AAGGTCGCCGAGGCCCTGAAGCTGATCCGCGACGAAGAGGCGGTGCGCCTGGTCAACGAGGAAGAGCTCAAG
GCACGCGCCCTGGAGGCGGTCGAGCAGCACGGCATCGTCTTCATCGACGAGATCGACAAGATCGCCAAGCGC
GCCAACGCCGGCGGCGCCGACGTCTCCCGCGAGGGCGTACAGCGCGACCTGCTGCCACTGATCGAGGGCTGC
ACGGTGAACACCAAGCTGGGCATGGTCAAGACCGACCACATCCTGTTCATCGCCTCCGGCGCCTTCCACCTG
AGCAAGCCGAGCGACCTGGTTCCCGAGCTGCAGGGCCGCCTGCCGATCCGCGTGGAGCTCAAGGCCCTCAGC
CCGAACGATTTCGAGCGCATCCTCACCGAGCCGCATGCCTCGCTCACCGAGCAGTACCGCGAGCTGCTGAAG
ACCGAGGGACTGGCCATCGAGTTCGCCGAGGACGGCATCAAGCGCCTTGCCGAGATCGCCTGGCAGGTCAAC
GAGAAGACCGAGAACATCGGTGCCCGCCGCCTGCATACGCTGCTTGAGCGGCTGCTGGAGGAGGTCTCGTTC
AGCGCAGCCGACCTGGCCAGCGAGCATAGCGACAAGCCGATCCTGATCGATGCCGGCTACGTCAACAGCCAC
CTCGGCGAGCTGGCCGAGGACGAGGACCTGTCCCGCTACATCCTTTGA
Translation:
MSMTPREIVHELNRHIIGQDDAKRAVAIALRNRWRRMQLPAELRAEVTPKNILMIGPTGVGKTEIARRLARL
ANAPFIKVEATKFTEVGYVGRDVESIIRDLADAAVKMLREQEIQKVKYRAEDAAEERILDALLPAARPAMGF
GDEPAREDSNTRQLFRKRLREGQLDDKEIDIEVADNPAGVEIMAPPGMEEMTNQLQNLFSGMSKGKKKTRKL
KVAEALKLIRDEEAVRLVNEEELKARALEAVEQHGIVFIDEIDKIAKRANAGGADVSREGVQRDLLPLIEGC
TVNTKLGMVKTDHILFIASGAFHLSKPSDLVPELQGRLPIRVELKALSPNDFERILTEPHASLTEQYRELLK
TEGLAIEFAEDGIKRLAEIAWQVNEKTENIGARRLHTLLERLLEEVSFSAADLASEHSDKPILIDAGYVNSH
LGELAEDEDLSRYIL*
AnnotationID:1227GeneID:1218
AnnotatorUID: diggins
Modification Date/Time: 2005-03-14 18:06:36
Gene Name:hslU
Confidence Code:2
GeneProduct:heat shock protein HslU
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(9) Chaperones & heat shock proteins
Alternate Gene Product Name:heat shock protein HslVU, ATPase subunit HslU
Functional Category Confidence Code:5
COGs:COG1220
Secondary Functional Category(ies):posttranslational modification, protein turnover, chaperones
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70567.1|AE016699_8 (AE016792) heat shock protein HslVU, ATPase subunit HslU
           [Pseudomonas putida KT2440]
          Length = 447

 Score =  748 bits (1931), Expect = 0.0
 Identities = 392/449 (87%), Positives = 415/449 (92%), Gaps = 4/449 (0%)

>gb|AAO58568.1| (AE016874) heat shock protein HslVU, ATPase subunit HslU
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 445

 Score =  744 bits (1920), Expect = 0.0
 Identities = 388/448 (86%), Positives = 413/448 (92%), Gaps = 4/448 (0%)

>gb|AAM83886.1|AE013628_9 (AE013628) heat shock protein, ATPase subunit [Yersinia pestis KIM]
          Length = 443

 Score =  634 bits (1634), Expect = 0.0
 Identities = 326/445 (73%), Positives = 379/445 (85%), Gaps = 5/445 (1%)






 
Structural Features:
COG1220, HslU, ATP-dependent protease HslVU (ClpYQ), ATPase subunit 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 444 residues,  99.8% aligned
Score =  695 bits (1795), Expect = 0.0
Identities = 318/448 (70%), Positives = 375/448 (83%), Gaps = 6/448 (1%)







   
Genomic Context:






   
Comment:


















    ReferenceID:2439




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19582




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35016




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1218_cdd.html


    		




 
URL:

















    ReferenceID:35832




  
Author/Investigator(s):
        Chuang SE, Burland V, Plunkett G 3rd, Daniels DL, Blattner FR.





  
Title:
        Sequence analysis of four new heat-shock genes constituting the hslTS/ibpAB and hslVU operons in Escherichia coli.




  
PubMed:
        8244018


MedLine:
        




    
Source:
Gene. 1993 Nov 30;134(1):1-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=94063501&dopt=Abstract

















    ReferenceID:35834




  
Author/Investigator(s):
        Lin M, Li Y.





  
Title:
        PCR genome walking identifies a genetic locus comprising two heat shock genes (hslV and hslU) from Leptospira borgpetersenii serovar hardjobovis.




  
PubMed:
        11685516


MedLine:
        




    
Source:
Curr Microbiol. 2001 Dec;43(6):452-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11685516









           
Homologs By Global Alignment 

           

       



     
Gene ID:1218
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2201
                           PA5054_tr
                           translation of PA5054
                           447
                           99.99
                           99.99
                           0
                           2228.0
     

     
     
73124
                           gb|AAG08439.1|AE004918_9
                           (AE004918) heat shock protein HslU [Pseudomonas aeruginosa PAO1]
                           447
                           99.99
                           99.99
                           0
                           2228.0
     

     
     
73125
                           gb|AAN70567.1|AE016699_8
                           (AE016792) heat shock protein HslVU, ATPase subunit HslU [Pseudomonas putida KT2440]
                           449
                           87.30
                           92.42
                           4
                           1937.0
     

     
     
73126
                           gb|AAO58568.1|
                           (AE016874) heat shock protein HslVU, ATPase subunit HslU [Pseudomonas syringae pv. tomato str. DC3000]
                           448
                           86.60
                           92.18
                           4
                           1926.0
     

     
     
73127
                           gb|AAM83886.1|AE013628_9
                           (AE013628) heat shock protein, ATPase subunit [Yersinia pestis KIM]
                           448
                           72.76
                           84.59
                           6
                           1638.0
     

     






      

          
GC ORFID: 44220How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5054Glimmer Score: 

          
Start: 5228205Stop: 5229548

          
Length: 1344


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5054 translation of PA5054Homolog Bit Score 862.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3790 (ORF: BLASTX 744.0), t_PA5054 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCATGACGCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGC 
GCCGTCGCCATCGCCCTGCGCAACCGCTGGCGACGCATGCAACTGCCGGCCGAGCTGCGCGCCGAGGTCACG 
CCGAAGAACATCCTCATGATCGGCCCCACCGGCGTCGGCAAGACCGAGATCGCCCGCCGCCTGGCGCGCCTG 
GCGAACGCGCCGTTCATCAAGGTCGAGGCGACCAAGTTCACCGAGGTCGGCTATGTCGGACGCGACGTCGAG 
TCGATCATCCGCGATCTCGCCGACGCCGCGGTGAAGATGCTCCGCGAACAGGAGATCCAGAAGGTCAAGTAT 
CGCGCCGAGGACGCCGCCGAAGAGCGCATCCTCGATGCCCTGCTGCCGGCCGCGCGTCCCGCCATGGGCTTC 
GGCGACGAGCCGGCACGCGAGGACAGCAACACCCGCCAGCTGTTCCGCAAGCGCCTGCGCGAAGGCCAGCTG 
GACGACAAGGAAATCGACATCGAGGTGGCCGACAACCCGGCCGGCGTGGAGATCATGGCCCCGCCCGGCATG 
GAGGAGATGACCAACCAGTTGCAGAACCTGTTCTCCGGCATGAGCAAGGGCAAGAAGAAGACCCGCAAGCTG 
AAGGTCGCCGAGGCCCTGAAGCTGATCCGCGACGAAGAGGCGGTGCGCCTGGTCAACGAGGAAGAGCTCAAG 
GCACGCGCCCTGGAGGCGGTCGAGCAGCACGGCATCGTCTTCATCGACGAGATCGACAAGATCGCCAAGCGC 
GCCAACGCCGGCGGCGCCGACGTCTCCCGCGAGGGCGTACAGCGCGACCTGCTGCCACTGATCGAGGGCTGC 
ACGGTGAACACCAAGCTGGGCATGGTCAAGACCGACCACATCCTGTTCATCGCCTCCGGCGCCTTCCACCTG 
AGCAAGCCGAGCGACCTGGTTCCCGAGCTGCAGGGCCGCCTGCCGATCCGCGTGGAGCTCAAGGCCCTCAGC 
CCGAACGATTTCGAGCGCATCCTCACCGAGCCGCATGCCTCGCTCACCGAGCAGTACCGCGAGCTGCTGAAG 
ACCGAGGGACTGGCCATCGAGTTCGCCGAGGACGGCATCAAGCGCCTTGCCGAGATCGCCTGGCAGGTCAAC 
GAGAAGACCGAGAACATCGGTGCCCGCCGCCTGCATACGCTGCTTGAGCGGCTGCTGGAGGAGGTCTCGTTC 
AGCGCAGCCGACCTGGCCAGCGAGCATAGCGACAAGCCGATCCTGATCGATGCCGGCTACGTCAACAGCCAC 
CTCGGCGAGCTGGCCGAGGACGAGGACCTGTCCCGCTACATCCTTTGA