ViewGene
Locus Name:
GeneID:
Location:

GeneID:1213
TrivialName: PA14_47760
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_47760
PAO1 Ortholog Locus:PA1273
Sequence Length:1308
Protein Length:435
Start: 4250235
Stop: 4248928
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04662.1|AE004557_3 (AE004557) cobyrinic acid a,c-diamide synthase [Pseudomonas aeruginosa PAO1]
Identities = 434/435 (99%), Positives = 434/435 (99%)
Sequence:
ATGACCCAGGCACGCTGTCCGGCCCTGCTGATCGCCGCCCCGGCCTCCGGCCAGGGCAAGACCACCGTCACC
GCCGCCCTGGCCCGCCTGCATGCGCGCCAGGGGCGACGGGTGCGGGTGTTCAAGTGCGGGCCGGACTTCCTC
GACCCGATGATTCTCGCCCGCGCCTCCGGCGCGCCGGTCTACCAGCTCGACCTGTGGATGGTCGGCGAGGCC
GAAGCGCGACGCCTGCTGGCGCGGGCCGCCGGCGAGGCCGACCTGATCCTCATCGAAGGGGTGATGGGCCTG
TTCGATGGCAACCCCTCGGCGGCAGACCTGGCGCGGCGTTTCGGCGTGCCGGTGCTGGGGGTGATCAACGGC
GCGGCGATGGCCCAGACCTTCGGCGCCCTGGCCTACGGCCTGGCGCATTTCCAGCCGGACCTGCCGTTTTCC
GGAGTGCTCGGCAACCGCGTCGGTAGCCAGCGCCACAGCGACATCCTGCGCGATTGCCTGCCGCCTGGCATG
CGCTGGTTCGGCGGCCTGCCGCGCTCGGCCGAGTTCGAACTGCCGAGCCGCCACCTGGGCCTGGTCCAGGCC
GAGGAACTGGCCGATCTCGATGTGCGCCTGGACGCCGCCGCCGACGCCTTGCGGGCCAGCGCCGAGACCGAC
CTGCCGGAGCCGGTGACCTTCGAGGTGCCCGCTCCGGCGCCGCTGCAACGCAGCCTGGAAGGCGTGCGCATC
GGCGTGGCTCGCGATGCCTCGTTCGCCTTCCTCTACCAGGCCAATCTCGACCTGCTGCGCGAACTCGGCGCC
GAACTGGCGTTCTTCTCGCCGCTGCAGGACCAGGCGTTGCCCGCCGTGGACAGCCTGTATCTGCCGGGCGGC
TACCCCGAACTGCACCTGGGGCGCCTGCAGGGCAATCGCGCCATGGCCGAGGCGATCCGAGCCCACCATGCC
GCCGGCAAGCCGCTGCTGGCCGAGTGCGGCGGCATGCTCTACCTGCTCGACTGCCTGGAAGACGCCGACGGC
GAGCGCGGCGAACTGCTCGGCCTGCTGCCGGGCCGGGCGCGCCTGCAGAAGCGCCTGACCGCCCTGGCCTTG
CAGGAGGTCGAGCTGCCGGAAGGGCGGCTGCGCGGGCATACCTTCCATCACTCGACCCTCGACTGCGCCGTC
GAGCCTCTGGCCCGTGGCGTCTGCCCGAACGGCCGGAACACGGCGGAGGCGGTGTTCCGTCTTGGCCGGCTG
ACGGCTTCCTATATCCATTTCTACCTGCCTTCCAACCCGCAGGCCGCCGCCGCGTTGCTGGCGCCCGCGCGA
GACGCCGACTGA
Translation:
MTQARCPALLIAAPASGQGKTTVTAALARLHARQGRRVRVFKCGPDFLDPMILARASGAPVYQLDLWMVGEA
EARRLLARAAGEADLILIEGVMGLFDGNPSAADLARRFGVPVLGVINGAAMAQTFGALAYGLAHFQPDLPFS
GVLGNRVGSQRHSDILRDCLPPGMRWFGGLPRSAEFELPSRHLGLVQAEELADLDVRLDAAADALRASAETD
LPEPVTFEVPAPAPLQRSLEGVRIGVARDASFAFLYQANLDLLRELGAELAFFSPLQDQALPAVDSLYLPGG
YPELHLGRLQGNRAMAEAIRAHHAAGKPLLAECGGMLYLLDCLEDADGERGELLGLLPGRARLQKRLTALAL
QEVELPEGRLRGHTFHHSTLDCAVEPLARGVCPNGRNTAEAVFRLGRLTASYIHFYLPSNPQAAAALLAPAR
DAD*
AnnotationID:1222GeneID:1213
AnnotatorUID: diggins
Modification Date/Time: 2005-02-22 18:06:50
Gene Name:cobB
Confidence Code:2
GeneProduct:cobyrinic acid a,c-diamide synthase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1797
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67294.1|AE016356_2 (AE016780) cobyrinic acid a,c-diamide synthase [Pseudomonas putida
           KT2440]
          Length = 431

 Score =  620 bits (1599), Expect = e-177
 Identities = 318/431 (73%), Positives = 346/431 (80%), Gaps = 1/431 (0%)

>gb|AAO55229.1| (AE016861) cobyrinic acid a,c-diamide synthase family protein
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 431

 Score =  598 bits (1543), Expect = e-170
 Identities = 301/425 (70%), Positives = 338/425 (79%)

>gb|AAM42334.1| (AE012421) cobyrinic acid a,c-diamide synthase [Xanthomonas
           campestris pv. campestris str. ATCC 33913]
          Length = 432

 Score =  520 bits (1338), Expect = e-147
 Identities = 267/428 (62%), Positives = 316/428 (73%), Gaps = 1/428 (0%)






 
Structural Features:
COG1797, CobB, Cobyrinic acid a,c-diamide synthase [Coenzyme metabolism]
CD-Length = 451 residues, 100.0% aligned
Score =  489 bits (1261), Expect = 2e-139
Identities = 205/451 (45%), Positives = 275/451 (60%), Gaps = 25/451 (5%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2429




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19572




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37509




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1213_cdd.html


    		




 
URL:

















    ReferenceID:38025




  
Author/Investigator(s):
        Crouzet J, Cauchois L, Blanche F, Debussche L, Thibaut D, Rouyez MC, Rigault S, Mayaux JF, Cameron B.





  
Title:
        Nucleotide sequence of a Pseudomonas denitrificans 5.4-kilobase DNA fragment containing five cob genes and identification of structural genes encoding S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase and cobyrinic acid a,c-diamide synthase.




  
PubMed:
        2211520


MedLine:
        




    
Source:
J Bacteriol. 1990 Oct;172(10):5968-79.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2211520









           
Homologs By Global Alignment 

           

       



     
Gene ID:1213
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2193
                           PA1273_tr
                           translation of PA1273
                           435
                           99.77
                           99.77
                           0
                           2226.0
     

     
     
73069
                           gb|AAG04662.1|AE004557_3
                           (AE004557) cobyrinic acid a,c-diamide synthase [Pseudomonas aeruginosa PAO1]
                           435
                           99.77
                           99.77
                           0
                           2226.0
     

     
     
73070
                           gb|AAN67294.1|AE016356_2
                           (AE016780) cobyrinic acid a,c-diamide synthase [Pseudomonas putida KT2440]
                           436
                           72.93
                           79.35
                           6
                           1601.0
     

     
     
73071
                           gb|AAO55229.1|
                           (AE016861) cobyrinic acid a,c-diamide synthase family protein [Pseudomonas syringae pv. tomato str. DC3000]
                           436
                           69.03
                           77.52
                           6
                           1540.0
     

     
     
73072
                           gb|AAM42334.1|
                           (AE012421) cobyrinic acid a,c-diamide synthase [Xanthomonas campestris pv. campestris str. ATCC 33913]
                           437
                           61.55
                           72.76
                           7
                           1339.0
     

     






      

          
GC ORFID: 44215How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1273Glimmer Score: 

          
Start: 703674Stop: 704981

          
Length: 1308


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1273 translation of PA1273Homolog Bit Score 862.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6469 (ORF: BLASTX 595.0), t_PA1273 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCCAGGCACGCTGTCCGGCCCTGCTGATCGCCGCCCCGGCCTCCGGCCAGGGCAAGACCACCGTCACC 
GCCGCCCTGGCCCGCCTGCATGCGCGCCAGGGGCGACGGGTGCGGGTGTTCAAGTGCGGGCCGGACTTCCTC 
GACCCGATGATTCTCGCCCGCGCCTCCGGCGCGCCGGTCTACCAGCTCGACCTGTGGATGGTCGGCGAGGCC 
GAAGCGCGACGCCTGCTGGCGCGGGCCGCCGGCGAGGCCGACCTGATCCTCATCGAAGGGGTGATGGGCCTG 
TTCGATGGCAACCCCTCGGCGGCAGACCTGGCGCGGCGTTTCGGCGTGCCGGTGCTGGGGGTGATCAACGGC 
GCGGCGATGGCCCAGACCTTCGGCGCCCTGGCCTACGGCCTGGCGCATTTCCAGCCGGACCTGCCGTTTTCC 
GGAGTGCTCGGCAACCGCGTCGGTAGCCAGCGCCACAGCGACATCCTGCGCGATTGCCTGCCGCCTGGCATG 
CGCTGGTTCGGCGGCCTGCCGCGCTCGGCCGAGTTCGAACTGCCGAGCCGCCACCTGGGCCTGGTCCAGGCC 
GAGGAACTGGCCGATCTCGATGTGCGCCTGGACGCCGCCGCCGACGCCTTGCGGGCCAGCGCCGAGACCGAC 
CTGCCGGAGCCGGTGACCTTCGAGGTGCCCGCTCCGGCGCCGCTGCAACGCAGCCTGGAAGGCGTGCGCATC 
GGCGTGGCTCGCGATGCCTCGTTCGCCTTCCTCTACCAGGCCAATCTCGACCTGCTGCGCGAACTCGGCGCC 
GAACTGGCGTTCTTCTCGCCGCTGCAGGACCAGGCGTTGCCCGCCGTGGACAGCCTGTATCTGCCGGGCGGC 
TACCCCGAACTGCACCTGGGGCGCCTGCAGGGCAATCGCGCCATGGCCGAGGCGATCCGAGCCCACCATGCC 
GCCGGCAAGCCGCTGCTGGCCGAGTGCGGCGGCATGCTCTACCTGCTCGACTGCCTGGAAGACGCCGACGGC 
GAGCGCGGCGAACTGCTCGGCCTGCTGCCGGGCCGGGCGCGCCTGCAGAAGCGCCTGACCGCCCTGGCCTTG 
CAGGAGGTCGAGCTGCCGGAAGGGCGGCTGCGCGGGCATACCTTCCATCACTCGACCCTCGACTGCGCCGTC 
GAGCCTCTGGCCCGTGGCGTCTGCCCGAACGGCCGGAACACGGCGGAGGCGGTGTTCCGTCTTGGCCGGCTG 
ACGGCTTCCTATATCCATTTCTACCTGCCTTCCAACCCGCAGGCCGCCGCCGCGTTGCTGGCGCCCGCGCGA 
GACGCCGACTGA