ViewGene
Locus Name:
GeneID:
Location:

GeneID:1211
TrivialName: PA14_34630
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_34630
PAO1 Ortholog Locus:PA2322
Sequence Length:1353
Protein Length:450
Start: 3076974
Stop: 3075622
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05710.1|AE004658_4 (AE004658) gluconate permease [Pseudomonas aeruginosa PAO1]
Identities = 450/450 (100%), Positives = 450/450 (100%)
Sequence:
ATGTTCGGCATGTCACACGATGCCTACCTGCTCCTCGACGCCCTGGTGACCATCATCGGCCTGATCGTGCTG
ATCACCCGCTTCAAGGTGCATCCCTTCATCGCCCTGATCATCGCCGCCGGCTTCCTCGGCCTGACCTCGGGG
ATGCCGGTGGAGAAGATCGTCAAATCCTTCCAGGACGGCTTCGGCGGCGTGCTCGGCTTCGTCGGCGTGATC
CTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGATTCCGGCGGCGCCGACCAGATCGCCCGCACCCTG
ATCCGCGCCTTCGGCAAGGAGCGGGTGCACTGGTCGATGATGCTCGCCGCGTTCCTGGTGGGCATCCCGCTG
TTCTTCGAGATCGGCTTCATCCTGCTGATCCCGCTGGTGTTCATCGTCGCCCGGCGCAGCGGCGTGTCCCTG
ATCAAGATCGGCATCCCGTTGCTCGCCGGCCTTTCCGCGGTGCACGGTCTGGTGCCGCCGCACCCCGGCCCG
CTGCTGGCGATCGGCGTGTTCGGCGCCGACATCGGCAAGACCATCCTCTACGGCCTGATCGTCGCCCTGCCC
ACCGCCGCCATCGCCGGCCCACTGTTCGGCGCGCTGGTCTCGCGCTACATCCCCGGCACGCCATCGGCGGAA
CTGGTCGAGCAGATCGCCCACGAGCCCGAGACCCAGGACCTGCCCAGCTTCGGCGTGACCCTGGCGACCGTG
CTGCTGCCGGTGTTCCTGATGCTGCTGAAGACCTTCGCCGACGTCGCCTTCCCCGATGGCCACGCGTTCCGC
GCCTGGATGGACATGATCGGCCATCCGATCAGCGCCCTGCTCCTGGCCCTGCTGGTGGCGCTCTATACCTTC
GGCTACGCCCGTGGCTTCGACTCGAAGAAGATCCTCAGGCTGCTCGACCAGAGCCTGGCACCGACCGCCGCC
ATCGTGATGATCATCGGCGCCGGCGGCGGCTTCAAGCAGATGCTGGTGGCCAGCGGGGTCGGCGACGTCATC
GGCCATCTCGCGGTGAACGCGCAGATCTCGCCGATCCTGCTGGCCTGGCTGGTGGCCGCGGTGATCCGCATC
GCCACCGGCTCGGCCACCGTCGCCACCATCACCGGGGCCGGCATCGTGGTGCCGGTGATCGACCTGATTCCC
GGCGTCAACCGCGAGTTGCTGGTACTCGCCACCGGCGCCGGCTCGCTGATCCTCTCCCATGTCAACGACGCC
GGGTTCTGGCTGGTCAAGCAGTACTTCAACATGAGCGTCAGCGAGACCTTCAAGACCTGGACCGCGATGGAG
ACCATCCTCTCCGTGGTCGGCCTGGTCTTCATCCTGTTGCTGTCGCTGGTGCTCTGA
Translation:
MFGMSHDAYLLLDALVTIIGLIVLITRFKVHPFIALIIAAGFLGLTSGMPVEKIVKSFQDGFGGVLGFVGVI
LALGTMLGKLMADSGGADQIARTLIRAFGKERVHWSMMLAAFLVGIPLFFEIGFILLIPLVFIVARRSGVSL
IKIGIPLLAGLSAVHGLVPPHPGPLLAIGVFGADIGKTILYGLIVALPTAAIAGPLFGALVSRYIPGTPSAE
LVEQIAHEPETQDLPSFGVTLATVLLPVFLMLLKTFADVAFPDGHAFRAWMDMIGHPISALLLALLVALYTF
GYARGFDSKKILRLLDQSLAPTAAIVMIIGAGGGFKQMLVASGVGDVIGHLAVNAQISPILLAWLVAAVIRI
ATGSATVATITGAGIVVPVIDLIPGVNRELLVLATGAGSLILSHVNDAGFWLVKQYFNMSVSETFKTWTAME
TILSVVGLVFILLLSLVL*
AnnotationID:1220GeneID:1211
AnnotatorUID: diggins
Modification Date/Time: 2005-04-29 07:07:07
Gene Name:gnuT
Confidence Code:2
GeneProduct:gluconate permease
Cell Localization:()
Synonyms:gntP
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:high-affinity gluconate transporter
Functional Category Confidence Code:5
COGs:pfam02447,COG2610
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57039.1| (AE016868) gluconate permease [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 450

 Score =  745 bits (1923), Expect = 0.0
 Identities = 366/450 (81%), Positives = 417/450 (92%)

>gb|AAP09190.1| (AE017005) Gluconate permease [Bacillus cereus ATCC 14579]
          Length = 452

 Score =  476 bits (1225), Expect = e-133
 Identities = 243/441 (55%), Positives = 316/441 (71%), Gaps = 7/441 (1%)

>dbj|BAC74339.1| (AP005047) putative gluconate permease [Streptomyces avermitilis
           MA-4680]
          Length = 465

 Score =  427 bits (1099), Expect = e-119
 Identities = 221/444 (49%), Positives = 307/444 (69%), Gaps = 2/444 (0%)






 
Structural Features:
pfam02447, GntP_permease, GntP family permease.
CD-Length = 441 residues, 100.0% aligned
Score =  493 bits (1272), Expect = 1e-140
Identities = 255/441 (57%), Positives = 332/441 (75%)

COG2610, GntT, H+/gluconate symporter and related permeases 
[Carbohydrate transport and metabolism / Amino acid transport and metabolism]
CD-Length = 442 residues, 100.0% aligned
Score =  351 bits (902), Expect = 1e-97
Identities = 238/445 (53%), Positives = 315/445 (70%), Gaps = 5/445 (1%)







   
Genomic Context:






   
Comment:


















    ReferenceID:2425




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19568




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32473




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1211_cdd.html


    		




 
URL:

















    ReferenceID:34463




  
Author/Investigator(s):
        Klemm P, Tong S, Nielsen H, Conway T.





  
Title:
        The gntP gene of Escherichia coli involved in gluconate uptake.




  
PubMed:
        8550444


MedLine:
        




    
Source:
J Bacteriol. 1996 Jan;178(1):61-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8550444

















    ReferenceID:34464




  
Author/Investigator(s):
        Peekhaus N, Tong S, Reizer J, Saier MH Jr, Murray E, Conway T.





  
Title:
        Characterization of a novel transporter family that includes multiple Escherichia coli gluconate transporters and their homologues.




  
PubMed:
        9119199


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1997 Feb 15;147(2):233-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9119199

















    ReferenceID:34465




  
Author/Investigator(s):
        Reizer A, Deutscher J, Saier MH Jr, Reizer J.





  
Title:
        Analysis of the gluconate (gnt) operon of Bacillus subtilis.




  
PubMed:
        1659648


MedLine:
        




    
Source:
Mol Microbiol. 1991 May;5(5):1081-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1659648

















    ReferenceID:34466




  
Author/Investigator(s):
        Fujita Y, Fujita T, Miwa Y, Nihashi J, Aratani Y.





  
Title:
        Organization and transcription of the gluconate operon, gnt, of Bacillus subtilis.




  
PubMed:
        3020045


MedLine:
        




    
Source:
J Biol Chem. 1986 Oct 15;261(29):13744-53.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3020045









           
Homologs By Global Alignment 

           

       



     
Gene ID:1211
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2191
                           PA2322_tr
                           translation of PA2322
                           450
                           99.99
                           99.99
                           0
                           2229.0
     

     
     
73009
                           gb|AAG05710.1|AE004658_4
                           (AE004658) gluconate permease [Pseudomonas aeruginosa PAO1]
                           450
                           99.99
                           99.99
                           0
                           2229.0
     

     
     
73010
                           gb|AAD01803.1|
                           (AF026470) gluconate permease [Pseudomonas aeruginosa]
                           450
                           99.99
                           99.99
                           0
                           2229.0
     

     
     
73011
                           gb|AAO57039.1|
                           (AE016868) gluconate permease [Pseudomonas syringae pv. tomato str. DC3000]
                           450
                           81.33
                           92.66
                           0
                           1923.0
     

     
     
73012
                           gb|AAN69019.1|AE016534_2
                           (AE016786) gluconate transporter [Pseudomonas putida KT2440]
                           450
                           80.66
                           91.55
                           0
                           1882.0
     

     






      

          
GC ORFID: 44213How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2322Glimmer Score: 

          
Start: 1876942Stop: 1878294

          
Length: 1353


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2322 translation of PA2322Homolog Bit Score 863.0

          
Other Homologs:  psyr_15may02_Scaffold9_revised_gene8171 (ORF: BLASTX 745.0), psyr_15may02_Scaffold2_revised_gene3770 (ORF: BLASTX 451.0), psyr_15may02_Scaffold3_revised_gene4457 (ORF: BLASTX 394.0), t_PA2322 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTTCGGCATGTCACACGATGCCTACCTGCTCCTCGACGCCCTGGTGACCATCATCGGCCTGATCGTGCTG 
ATCACCCGCTTCAAGGTGCATCCCTTCATCGCCCTGATCATCGCCGCCGGCTTCCTCGGCCTGACCTCGGGG 
ATGCCGGTGGAGAAGATCGTCAAATCCTTCCAGGACGGCTTCGGCGGCGTGCTCGGCTTCGTCGGCGTGATC 
CTCGCCCTGGGCACCATGCTCGGCAAGCTGATGGCCGATTCCGGCGGCGCCGACCAGATCGCCCGCACCCTG 
ATCCGCGCCTTCGGCAAGGAGCGGGTGCACTGGTCGATGATGCTCGCCGCGTTCCTGGTGGGCATCCCGCTG 
TTCTTCGAGATCGGCTTCATCCTGCTGATCCCGCTGGTGTTCATCGTCGCCCGGCGCAGCGGCGTGTCCCTG 
ATCAAGATCGGCATCCCGTTGCTCGCCGGCCTTTCCGCGGTGCACGGTCTGGTGCCGCCGCACCCCGGCCCG 
CTGCTGGCGATCGGCGTGTTCGGCGCCGACATCGGCAAGACCATCCTCTACGGCCTGATCGTCGCCCTGCCC 
ACCGCCGCCATCGCCGGCCCACTGTTCGGCGCGCTGGTCTCGCGCTACATCCCCGGCACGCCATCGGCGGAA 
CTGGTCGAGCAGATCGCCCACGAGCCCGAGACCCAGGACCTGCCCAGCTTCGGCGTGACCCTGGCGACCGTG 
CTGCTGCCGGTGTTCCTGATGCTGCTGAAGACCTTCGCCGACGTCGCCTTCCCCGATGGCCACGCGTTCCGC 
GCCTGGATGGACATGATCGGCCATCCGATCAGCGCCCTGCTCCTGGCCCTGCTGGTGGCGCTCTATACCTTC 
GGCTACGCCCGTGGCTTCGACTCGAAGAAGATCCTCAGGCTGCTCGACCAGAGCCTGGCACCGACCGCCGCC 
ATCGTGATGATCATCGGCGCCGGCGGCGGCTTCAAGCAGATGCTGGTGGCCAGCGGGGTCGGCGACGTCATC 
GGCCATCTCGCGGTGAACGCGCAGATCTCGCCGATCCTGCTGGCCTGGCTGGTGGCCGCGGTGATCCGCATC 
GCCACCGGCTCGGCCACCGTCGCCACCATCACCGGGGCCGGCATCGTGGTGCCGGTGATCGACCTGATTCCC 
GGCGTCAACCGCGAGTTGCTGGTACTCGCCACCGGCGCCGGCTCGCTGATCCTCTCCCATGTCAACGACGCC 
GGGTTCTGGCTGGTCAAGCAGTACTTCAACATGAGCGTCAGCGAGACCTTCAAGACCTGGACCGCGATGGAG 
ACCATCCTCTCCGTGGTCGGCCTGGTCTTCATCCTGTTGCTGTCGCTGGTGCTCTGA