ViewGene
Locus Name:
GeneID:
Location:

GeneID:1204
TrivialName: PA14_65230
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_65230
PAO1 Ortholog Locus:PA4938
Sequence Length:1293
Protein Length:430
Start: 5814755
Stop: 5813463
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08323.1|AE004907_1 (AE004907) adenylosuccinate synthetase [Pseudomonas aeruginosa PAO1]
Identities = 430/430 (100%), Positives = 430/430 (100%)
Sequence:
ATGGGTAAGAATGTCGTAGTCCTGGGCACCCAATGGGGTGATGAGGGCAAGGGCAAGATCGTCGACCTGCTC
ACCGAGCAGGCGGCTGCCGTAGTTCGCTACCAGGGTGGTCACAACGCTGGCCACACCCTGGTGATCGATGGC
GAGAAAACCGTCCTGCACCTGATTCCATCGGGCATCCTGCGCGAAGGCGTGCAGTGCCTGATCGGCAATGGC
GTGGTGCTGGCGCCGGATGCGCTGCTGCGCGAAATCACCAAGCTGGAAGAGAAGGGCGTGCCGGTGCGCGAG
CGCCTGCGCATCAGTCCTTCCTGTCCGCTGATCCTGTCCTATCACGTCGCCCTCGACCAGGCGCGCGAGAAG
GCCCGCGGCGAAGCCAAGATCGGCACCACCGGTCGCGGTATCGGCCCGGCATACGAGGACAAGGTGGCACGT
CGCGGCCTGCGCGTTGGCGACCTGTTCCACCGCGAGCGCTTCGCCGCCAAGCTGGGCGAGCTGCTGGACTAC
CATAACTTCGTCCTGCAGCACTACTACAAGGAGCCGGCCATCGACTTCCAGAAGACTCTGGACGAGGCGATG
GAGTATGCCGAGCTGCTCAAGCCGATGATGGCCGATGTCGCTGCGACCCTGCACGACCTGCGCAAGCACGGC
AAGGACATCATGTTCGAAGGCGCCCAGGGATCGCTGCTGGACATCGACCATGGCACCTATCCCTACGTGACC
AGTTCCAACACCACCGCTGGCGGTACCGCCACCGGTTCCGGCTTCGGCCCGCTGTACCTGGACTACGTACTG
GGTATCACCAAGGCCTATACCACTCGCGTCGGCTCCGGTCCGTTCCCGACCGAGCTGTTCGACGACGTCGGC
GCCTACCTGGCCAAGCGCGGCCACGAGTTCGGCGCCACCACCGGTCGCGCTCGTCGCTGCGGCTGGTTCGAT
GCCGTGATCCTGCGCCGCGCCATCGAGATCAACAGCATCTCCGGCCTGTGCCTGACCAAGCTCGACGTGCTC
GATGGTCTGGACGTGGTGCGCCTGTGCGTCGGCTACAAGAATGCCGATGGCGATGTGCTGGAAGCGCCGACC
GATGCCGACAGCTACATCGGCCTGCAGCCGGTCTACGAGGAAATGCCCGGCTGGAGCGAGTCGACCGTGGGT
GCGAAGACCCTGGAAGAGTTGCCGGCCAACGCCCGCGCCTACATCAAGCGCGTGGAAGAACTGGTGGGTGCG
CCGATCGATATCATCTCCACCGGTCCGGATCGCAACGAAACCATCATCCTGCGCCATCCATTCGCCTGA
Translation:
MGKNVVVLGTQWGDEGKGKIVDLLTEQAAAVVRYQGGHNAGHTLVIDGEKTVLHLIPSGILREGVQCLIGNG
VVLAPDALLREITKLEEKGVPVRERLRISPSCPLILSYHVALDQAREKARGEAKIGTTGRGIGPAYEDKVAR
RGLRVGDLFHRERFAAKLGELLDYHNFVLQHYYKEPAIDFQKTLDEAMEYAELLKPMMADVAATLHDLRKHG
KDIMFEGAQGSLLDIDHGTYPYVTSSNTTAGGTATGSGFGPLYLDYVLGITKAYTTRVGSGPFPTELFDDVG
AYLAKRGHEFGATTGRARRCGWFDAVILRRAIEINSISGLCLTKLDVLDGLDVVRLCVGYKNADGDVLEAPT
DADSYIGLQPVYEEMPGWSESTVGAKTLEELPANARAYIKRVEELVGAPIDIISTGPDRNETIILRHPFA*
AnnotationID:1213GeneID:1204
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 07:07:33
Gene Name:purA
Confidence Code:2
GeneProduct:adenylosuccinate synthetase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(18) Nucleotide biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:cd00410
Secondary Functional Category(ies):central intermediary metabolism
EC Number:6.3.4.4
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58365.1| (AE016873) adenylosuccinate synthetase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 430

 Score =  802 bits (2071), Expect = 0.0
 Identities = 387/429 (90%), Positives = 413/429 (96%)

>gb|AAN70456.1|AE016687_3 (AE016792) adenylosuccinate synthetase [Pseudomonas putida KT2440]
          Length = 430

 Score =  780 bits (2015), Expect = 0.0
 Identities = 380/430 (88%), Positives = 407/430 (94%)

>gb|AAF95743.1| (AE004327) adenylosuccinate synthetase [Vibrio cholerae]
          Length = 432

 Score =  627 bits (1617), Expect = e-179
 Identities = 304/431 (70%), Positives = 354/431 (82%), Gaps = 1/431 (0%)






 
Structural Features:
cd00410, Adenylsucc_synt, Adenylosuccinate synthetase
CD-Length = 418 residues, 100.0% aligned
Score =  595 bits (1535), Expect = 4e-171
Identities = 262/422 (62%), Positives = 310/422 (73%), Gaps = 4/422 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2411




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19554




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35119




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1204_cdd.html


    		




 
URL:

















    ReferenceID:35908




  
Author/Investigator(s):
        Wolfe SA, Smith JM.





  
Title:
        Nucleotide sequence and analysis of the purA gene encoding adenylosuccinate synthetase of Escherichia coli K12.




  
PubMed:
        3058695


MedLine:
        




    
Source:
J Biol Chem. 1988 Dec 15;263(35):19147-53.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3058695

















    ReferenceID:35909




  
Author/Investigator(s):
        Mantsala P, Zalkin H.





  
Title:
        Cloning and sequence of Bacillus subtilis purA and guaA, involved in the conversion of IMP to AMP and GMP.




  
PubMed:
        1312531


MedLine:
        




    
Source:
J Bacteriol. 1992 Mar;174(6):1883-90.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1312531

















    ReferenceID:35910




  
Author/Investigator(s):
        Kusano T, Takeshima T, Inoue C, Sugawara K.





  
Title:
        Identification of the purA gene encoding adenylosuccinate synthetase in Thiobacillus ferrooxidans.




  
PubMed:
        7763499


MedLine:
        




    
Source:
Curr Microbiol. 1993 Apr;26(4):197-204.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7763499









           
Homologs By Global Alignment 

           

       



     
Gene ID:1204
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2182
                           PA4938_tr
                           translation of PA4938
                           430
                           99.99
                           99.99
                           0
                           2234.0
     

     
     
72642
                           gb|AAG08323.1|AE004907_1
                           (AE004907) adenylosuccinate synthetase [Pseudomonas aeruginosa PAO1]
                           430
                           99.99
                           99.99
                           0
                           2234.0
     

     
     
72643
                           gb|AAO58365.1|
                           (AE016873) adenylosuccinate synthetase [Pseudomonas syringae pv. tomato str. DC3000]
                           430
                           90.00
                           96.04
                           0
                           2071.0
     

     
     
72644
                           gb|AAN70456.1|AE016687_3
                           (AE016792) adenylosuccinate synthetase [Pseudomonas putida KT2440]
                           430
                           88.37
                           94.65
                           0
                           2015.0
     

     
     
72645
                           emb|CAE16949.1|
                           (BX571874) adenylosuccinate synthetase (IMP--aspartate ligase) (ADSS) (AMPSASE) [Photorhabdus luminescens subsp. laumondii TTO1]
                           432
                           71.29
                           82.63
                           2
                           1633.0
     

     






      

          
GC ORFID: 44206How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4938Glimmer Score: 

          
Start: 5078436Stop: 5077144

          
Length: 1293


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4938 translation of PA4938Homolog Bit Score 865.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3435 (ORF: BLASTX 801.0), t_PA4938 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGGTAAGAATGTCGTAGTCCTGGGCACCCAATGGGGTGATGAGGGCAAGGGCAAGATCGTCGACCTGCTC 
ACCGAGCAGGCGGCTGCCGTAGTTCGCTACCAGGGTGGTCACAACGCTGGCCACACCCTGGTGATCGATGGC 
GAGAAAACCGTCCTGCACCTGATTCCATCGGGCATCCTGCGCGAAGGCGTGCAGTGCCTGATCGGCAATGGC 
GTGGTGCTGGCGCCGGATGCGCTGCTGCGCGAAATCACCAAGCTGGAAGAGAAGGGCGTGCCGGTGCGCGAG 
CGCCTGCGCATCAGTCCTTCCTGTCCGCTGATCCTGTCCTATCACGTCGCCCTCGACCAGGCGCGCGAGAAG 
GCCCGCGGCGAAGCCAAGATCGGCACCACCGGTCGCGGTATCGGCCCGGCATACGAGGACAAGGTGGCACGT 
CGCGGCCTGCGCGTTGGCGACCTGTTCCACCGCGAGCGCTTCGCCGCCAAGCTGGGCGAGCTGCTGGACTAC 
CATAACTTCGTCCTGCAGCACTACTACAAGGAGCCGGCCATCGACTTCCAGAAGACTCTGGACGAGGCGATG 
GAGTATGCCGAGCTGCTCAAGCCGATGATGGCCGATGTCGCTGCGACCCTGCACGACCTGCGCAAGCACGGC 
AAGGACATCATGTTCGAAGGCGCCCAGGGATCGCTGCTGGACATCGACCATGGCACCTATCCCTACGTGACC 
AGTTCCAACACCACCGCTGGCGGTACCGCCACCGGTTCCGGCTTCGGCCCGCTGTACCTGGACTACGTACTG 
GGTATCACCAAGGCCTATACCACTCGCGTCGGCTCCGGTCCGTTCCCGACCGAGCTGTTCGACGACGTCGGC 
GCCTACCTGGCCAAGCGCGGCCACGAGTTCGGCGCCACCACCGGTCGCGCTCGTCGCTGCGGCTGGTTCGAT 
GCCGTGATCCTGCGCCGCGCCATCGAGATCAACAGCATCTCCGGCCTGTGCCTGACCAAGCTCGACGTGCTC 
GATGGTCTGGACGTGGTGCGCCTGTGCGTCGGCTACAAGAATGCCGATGGCGATGTGCTGGAAGCGCCGACC 
GATGCCGACAGCTACATCGGCCTGCAGCCGGTCTACGAGGAAATGCCCGGCTGGAGCGAGTCGACCGTGGGT 
GCGAAGACCCTGGAAGAGTTGCCGGCCAACGCCCGCGCCTACATCAAGCGCGTGGAAGAACTGGTGGGTGCG 
CCGATCGATATCATCTCCACCGGTCCGGATCGCAACGAAACCATCATCCTGCGCCATCCATTCGCCTGA