ViewGene
Locus Name:
GeneID:
Location:

GeneID:1202
TrivialName: PA14_19230
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_19230
PAO1 Ortholog Locus:PA3468
Sequence Length:1329
Protein Length:442
Start: 1660546
Stop: 1659218
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06856.1|AE004768_1 (AE004768) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 440/441 (99%), Positives = 440/441 (99%)
Sequence:
ATGGACTCCACATCCCTTCTCGACCAGCTCTCCGGGTACTGGTCGGCGCTCGACCAACACCCTATCCTGCAC
GCCGGCCTGGCCTTCGGCCTGCTGCTGGTGGGCGCCCTGATTCTCGGCCGGGTGGCCCGCTACATCGTCCTC
TACGCCGCCAACCTGCTCGCCCGCCAGCCCGCGCTGCACTGGCTCGGCGACCTGATCCGCAACAAGGTGTTC
CAGCGCCTGGCACAGACCACGCCCTCGCTGCTGGTGCAATTCGGCCTGCCATGGATCCCGGAGATGCCGGAC
AAGGCCGCGCACTTCCTCGGCAACCTGGCACTGGCCTTCACCGTGCTGTTCCTGATCCTCGCCCTCACCGCG
CTGCTGAATGCCCTGCTGGACATCTACGCGCGTACCGAGCATGCCCGCACCCGTTCGATCAAGGGCTACGTG
CAACTGGCGAAGATCGCCGTGTTCGTGTTCGGCGCGATCATCATCGTCTCGATCATCATCGACCGCTCGCCG
CTCCTGCTGCTGTCCGGCCTCGGCGCGATGTCGGCGGTCCTCCTGCTGGTCTACAAGGACACCCTGATGTCC
TTCGTCGCCAGCGTGCAGCTCACCAGCAACGACATGCTGCGGGTCGGCGACTGGATCGAGATGCCGCAGGTC
GGCGCCGACGGCGACGTGGTGGACATCACCCTGTACACCGTGAAGGTGCAGAACTTCGACAAGACCATCGTC
TCGGTGCCGACCTGGCGCCTGATGTCGGAGTCGTTCAAGAACTGGCGCGGCATGCAGCAGTCCGGCGGACGG
CGGATCAAGCGGGCAATCTACGTCGACTCCAGCCAGGTGCGTTTCCTCGACGAGAAGGAAGAGCGGCGCCTG
AGCCAGGTGCGCCTGCTCACCGAATACATGGCCCGCAAGCAGACCGAGTTGCAGGAGTGGAATGCCGCCAAC
GGCGGGGTCGCCCCGCTGGCCGCCAACCGCCGCCGGCTGACCAACATCGGTACGTTCCGCGCCTACGCCCTC
GCCTACCTGCAGAACCACCCGGACATCCATCCGCACATGACCTGCATGGTCCGCCAGTTGCAGCCGACAGCC
CAGGGCATCCCGCTGGAGATATACTGCTTCACCCGCACCACCGCCTGGGTCGACTACGAGCGCATCCAGGGC
GACGTGTTCGACTACCTGCTGGCGGTGATGCCGGAGTTCGGCCTCGGCGCCTACCAGCAGCCCAGCGGCCAG
GACCTGCGCCAGGGGCTGCAAGGCATGCTCGAAGGGCGTGACGCCGGGGCCCGCTCGTTCGAGGCGCTGGCC
GCGCCGAGCCGCAGCGACGAAGCCACCGCCTGA
Translation:
MDSTSLLDQLSGYWSALDQHPILHAGLAFGLLLVGALILGRVARYIVLYAANLLARQPALHWLGDLIRNKVF
QRLAQTTPSLLVQFGLPWIPEMPDKAAHFLGNLALAFTVLFLILALTALLNALLDIYARTEHARTRSIKGYV
QLAKIAVFVFGAIIIVSIIIDRSPLLLLSGLGAMSAVLLLVYKDTLMSFVASVQLTSNDMLRVGDWIEMPQV
GADGDVVDITLYTVKVQNFDKTIVSVPTWRLMSESFKNWRGMQQSGGRRIKRAIYVDSSQVRFLDEKEERRL
SQVRLLTEYMARKQTELQEWNAANGGVAPLAANRRRLTNIGTFRAYALAYLQNHPDIHPHMTCMVRQLQPTA
QGIPLEIYCFTRTTAWVDYERIQGDVFDYLLAVMPEFGLGAYQQPSGQDLRQGLQGMLEGRDAGARSFEALA
APSRSDEATA*
AnnotationID:1211GeneID:1202
AnnotatorUID: diggins
Modification Date/Time: 2005-08-14 09:09:02
Gene Name:
Confidence Code:3
GeneProduct:putative mechanosensitive ion channel family protein
Cell Localization:()
Synonyms:ybdG
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(15) Membrane proteins
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00924,COG0668
Secondary Functional Category(ies):transport of small molecules
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55094.1| (AE016861) mechanosensitive ion channel family protein [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 436

 Score =  611 bits (1575), Expect = e-174
 Identities = 303/429 (70%), Positives = 357/429 (83%), Gaps = 1/429 (0%)

>emb|CAE33002.1| (BX640444) putative membrane transport protein [Bordetella
           bronchiseptica]
          Length = 448

 Score =  389 bits (999), Expect = e-107
 Identities = 199/394 (50%), Positives = 275/394 (69%), Gaps = 2/394 (0%)

>gb|AAN42134.1|AE015080_2 (AE015080) putative transport [Shigella flexneri 2a str. 301]
          Length = 421

 Score =  344 bits (882), Expect = 7e-94
 Identities = 169/385 (43%), Positives = 255/385 (66%), Gaps = 1/385 (0%)






 
Structural Features:
pfam00924, MS_channel, Mechanosensitive ion channel. 
CD-Length = 205 residues,  98.0% aligned
Score = 94.1 bits (234), Expect = 3e-20
Identities = 44/255 (17%), Positives = 96/255 (37%), Gaps = 54/255 (21%)

COG0668, MscS, Small-conductance mechanosensitive channel 
[Cell envelope biogenesis, outer membrane]
CD-Length = 316 residues,  89.9% aligned
Score =  107 bits (268), Expect = 3e-24
Identities = 61/317 (19%), Positives = 105/317 (33%), Gaps = 40/317 (12%)







   
Genomic Context:






   
Comment:


















    ReferenceID:2407




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19550




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32090




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1202_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:1202
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2180
                           PA3468_tr
                           translation of PA3468
                           442
                           99.54
                           99.54
                           0
                           2234.0
     

     
     
72577
                           gb|AAG06856.1|AE004768_1
                           (AE004768) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           442
                           99.54
                           99.54
                           0
                           2234.0
     

     
     
72578
                           gb|AAN70107.1|AE016650_6
                           (AE016791) conserved hypothetical protein [Pseudomonas putida KT2440]
                           443
                           68.84
                           83.52
                           11
                           1595.0
     

     
     
72579
                           gb|AAO55094.1|
                           (AE016861) mechanosensitive ion channel family protein [Pseudomonas syringae pv. tomato str. DC3000]
                           442
                           68.55
                           80.99
                           6
                           1575.0
     

     
     
72582
                           emb|CAE36736.1|
                           (BX640427) putative membrane transport protein [Bordetella parapertussis]
                           459
                           46.40
                           63.61
                           28
                           1002.0
     

     






      

          
GC ORFID: 44204How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3468Glimmer Score: 

          
Start: 3293386Stop: 3294714

          
Length: 1329


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3468 translation of PA3468Homolog Bit Score 865.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6028 (ORF: BLASTX 613.0), psyr_15may02_Scaffold8_revised_gene7631 (ORF: BLASTX 196.0), t_PA3468 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGACTCCACATCCCTTCTCGACCAGCTCTCCGGGTACTGGTCGGCGCTCGACCAACACCCTATCCTGCAC 
GCCGGCCTGGCCTTCGGCCTGCTGCTGGTGGGCGCCCTGATTCTCGGCCGGGTGGCCCGCTACATCGTCCTC 
TACGCCGCCAACCTGCTCGCCCGCCAGCCCGCGCTGCACTGGCTCGGCGACCTGATCCGCAACAAGGTGTTC 
CAGCGCCTGGCACAGACCACGCCCTCGCTGCTGGTGCAATTCGGCCTGCCATGGATCCCGGAGATGCCGGAC 
AAGGCCGCGCACTTCCTCGGCAACCTGGCACTGGCCTTCACCGTGCTGTTCCTGATCCTCGCCCTCACCGCG 
CTGCTGAATGCCCTGCTGGACATCTACGCGCGTACCGAGCATGCCCGCACCCGTTCGATCAAGGGCTACGTG 
CAACTGGCGAAGATCGCCGTGTTCGTGTTCGGCGCGATCATCATCGTCTCGATCATCATCGACCGCTCGCCG 
CTCCTGCTGCTGTCCGGCCTCGGCGCGATGTCGGCGGTCCTCCTGCTGGTCTACAAGGACACCCTGATGTCC 
TTCGTCGCCAGCGTGCAGCTCACCAGCAACGACATGCTGCGGGTCGGCGACTGGATCGAGATGCCGCAGGTC 
GGCGCCGACGGCGACGTGGTGGACATCACCCTGTACACCGTGAAGGTGCAGAACTTCGACAAGACCATCGTC 
TCGGTGCCGACCTGGCGCCTGATGTCGGAGTCGTTCAAGAACTGGCGCGGCATGCAGCAGTCCGGCGGACGG 
CGGATCAAGCGGGCAATCTACGTCGACTCCAGCCAGGTGCGTTTCCTCGACGAGAAGGAAGAGCGGCGCCTG 
AGCCAGGTGCGCCTGCTCACCGAATACATGGCCCGCAAGCAGACCGAGTTGCAGGAGTGGAATGCCGCCAAC 
GGCGGGGTCGCCCCGCTGGCCGCCAACCGCCGCCGGCTGACCAACATCGGTACGTTCCGCGCCTACGCCCTC 
GCCTACCTGCAGAACCACCCGGACATCCATCCGCACATGACCTGCATGGTCCGCCAGTTGCAGCCGACAGCC 
CAGGGCATCCCGCTGGAGATATACTGCTTCACCCGCACCACCGCCTGGGTCGACTACGAGCGCATCCAGGGC 
GACGTGTTCGACTACCTGCTGGCGGTGATGCCGGAGTTCGGCCTCGGCGCCTACCAGCAGCCCAGCGGCCAG 
GACCTGCGCCAGGGGCTGCAAGGCATGCTCGAAGGGCGTGACGCCGGGGCCCGCTCGTTCGAGGCGCTGGCC 
GCGCCGAGCCGCAGCGACGAAGCCACCGCCTGA