ViewGene

Locus Name:
GeneID:
Location:

GeneID: 12

TrivialName: PA14_66570

AnnotatorUID:

Modification Date/Time: 2006-04-06 13:01:23

LocusName: PA14_66570

PAO1 Ortholog Locus: PA5036

Sequence Length: 4446

Protein Length: 1481

Start: 5943543

Stop: 5939098

Strand: -

Type:

ChromosomeID: 2

Status: ACTIVE

Frame Discrepancy: FALSE

Missense Discrepancy: FALSE

Comments:
Homology: gb|AAG08421.1|AE004916_10 (AE004916) glutamate synthase large chain precursor [Pseudomonas aeruginosa PAO1] Identities = 1479/1481 (99%), Positives = 1480/1481 (99%)

Sequence:

ATGAAAGCAGGTCTGTACCATCCTGAAACGTTCAAGGATAACTGCGGATTTGGCCTGATCGCCCATATGCAG
GGCGAGCCGAGCCATCAACTTCTGCAAACCGCAATCGAAGCACTGACCTGCATGACCCACCGCGGTGGGATC
AATGCCGATGGCAAGACCGGGGACGGCTGCGGGCTGCTGATCCAGAAGCCCGACCTGTTCCTGCGTGCCGTC
GCCAGGGAAGCCTTTTCTGTCGACCTGCCCGAGCAATACGCGGTGGGCATGGTGTTCTTCAACCAGGACCCG
GTGAAAGCCGAGGCGGCACGCGAGAACATGAATCGCGAGATCGTCGCCGCCGGACTGGAGCTGGTCGGCTGG
CGCAAGGTGCCGATCGATACCAGCGTTCTCGGCCGCCTGGCGCTGGAGCGCCTGCCGCAGATCGAACAGGTG
TTCATCGGCGGCGCCGGCCTGAGCGACCAGGACTTCGCCATCAAGCTGTTCAGCGCACGGCGCCGCTCGTCG
GTGGCCAACGCCGCCGACAGCGACCACTACATCTGCAGCTTTTCGCACAAGACCATCATCTATAAGGGCCTG
ATGATGCCGGCCGACCTGGCCGCCTTCTATCCGGACCTTGGCGACGAGCGCCTGCAGACCGCGATCTGCGTA
TTCCACCAGCGCTTCTCCACCAACACCCTGCCGAAATGGCCCCTGGCGCAGCCTTTCCGCTTCCTCGCCCAC
AACGGCGAGATCAACACCATCACCGGCAACCGCAACTGGGCCCAGGCGCGGCGGACCAAGTTCACCAACGAA
CTGATCCCCGACCTGGAAGAGCTAGGCCCGCTGGTGAACCGCGTCGGCTCCGACTCGTCGAGCATGGACAAC
ATGCTCGAACTGATGGTCACCGGCGGCATGGACCTGTTCCGCGGCCTGCGCATGATCATCCCGCCGGCCTGG
CAGAACGTCGAGACCATGGACGCCGACCTGCGCGCGTTCTACGAGTATAACTCGCTGCACATGGAGCCCTGG
GATGGCCCCGCCGGCGTCGTGCTGACCGACGGTCGCTATGCCGTCTGCCTGCTCGACCGCAATGGCCTGCGC
CCGTCGCGCTGGGTCACCACGAAGAACGGCTACATCACCCTGGCCTCGGAGATCGGCGTCTGGGACTACAAG
CCCGAGGACGTCATCGCCAAGGGTCGCGTCGGTCCGGGGCAGATCCTCGCGGTGGACACCGAGACCGGCCAG
GTCCTGCACAGCGACGACATCGACAACCGCCTGAAGTCGCGCCATCCCTACAAGCAATGGCTGCGGCAGAAT
GCCCTGCGCATCCAGGCGACCCTGGACGACGACCATGGCGTGGCCAGCTACGACGCCGACCAGCTCAAGCAA
TTCATGAAGATGTTCCAGGTCACCTTCGAGGAGCGTGACCAGGTGCTGCGTCCGCTCGGCGAGCAGGGCCAG
GAAGCGGTGGGCTCCATGGGCGACGATACGCCGATGGCGATCCTCTCCCGGCGCATCCGCTCTCCGTACGAT
TACTTCCGCCAGCAATTCGCCCAGGTCACCAACCCGCCGATCGACCCGCTGCGCGAAGCCATCGTGATGTCC
CTGGAAACCTGCCTGGGCGTCGAGCAGAACATCTTCGAGGAAGCCCCGCACCATGCCAACCAGGCGATCCTC
ACCACTCCGGTGATCTCGCCGGCGAAGTGGCGGACCATCATGACCCTGGACCGCCCGGGCTTCGACCGCCAC
TTCATCGACCTCAACTACGACGAGTCGATGGGCCTGGAGGCCGCGGTGCGCAACATCGCCGACCAGGCCGAG
GAAGCCGTGCGCGGCGGCAAGGTGCTGCTGATCCTCAGCGACCGCCATATCGCTCCCGGCAAACTGCCGGTG
CATGCTGCCCTGGCCGTCGGCGCGGTGCATCACCGCCTGGTGCAGACCGGGCTGCGCTGCGACTCCAACATC
CTGGTGGAGACCGCCACCGCCCGCGATCCGCACCATTTCGCGGTGCTGATCGGCTTCGGCGCCTCGGCGGTC
TATCCGTTCCTCGCCTACGAGGTGCTGGCCGACCTGATCCGCACCGGTGAAGTGCTGGGCGACCTCTACGAG
GTGTTCAAGTACTACCGCAAGGGGATCTCCAAGGGCCTGATGAAGATTCTCTCGAAGATGGGCATCTCCACC
GTCGCGTCCTACCGTGGCGCGCAGCTGTTCGAGGCGGTGGGCCTGTCCGACGAGGTCACCGGCCTGTGCTTC
ACCGGAGTCGCCAGCCGCATCCAGGGTGCGCGCTTCGTCGATATCGAGAACGAGCAGAAGCTCCTGGCCGCC
GAGGCCTGGAGCAACCGCAAGCCGATCCAGCAGGGCGGCTTGCTGAAGTTCGTCTATGGCGGCGAATACCAC
GCCTACAACCCGGATGTGGTGAATACCCTGCAGGCTGCCGTGCAGCAGGGCGACTACGAGAAGTTCAAGGAA
TACACCGCGCTGGTGGACCAGCGCCCGGTGTCGATGATCCGCGACCTGTTGCAGGTGAAGACCGCCGCGCAG
CCGCTGGCGCTCGACGAGGTGGAGCCGCTGGAAGCCATCTTCAAGCGCTTCGATGCCGCCGGGATTTCCCTC
GGCGCGCTGTCGCCGGAGGCCCACGAGGCGCTGGCCGAGGCGATGAACACCCTGGGCGGTCGTTCCAACTCC
GGCGAGGGCGGCGAGGACCCGGCGCGCTACGGCACGCTGAAGAGTTCGAAGATCAAGCAGGTGGCTACCGGC
CGCTTCGGCGTGACCCCGGAATACCTGGTCAACGCCGAGGTCCTGCAGATCAAGgTGGCCCAGGGCGCCAAG
CCCGGCGAGGGCGGCCAGTTGCCGGGCGGCAAGGTCAACGGCCTGATCGCCCGCCTGCGCTATGCGGTGCCC
GGCGTGACCCTGATCTCGCCGCCGCCGCACCACGACATCTACTCCATCGAAGACCTGGCGCAGCTGATCTTC
GACCTCAAGCAGGTCAACCCGCAGGCGCTGGTATCGGTGAAGCTGGTATCCGAGCCTGGCGTCGGCACCATC
GCCGCCGGCGTGGCCAAGGCCTATGCGGACTTGATCACCATCTCCGGCTACGACGGCGGTACCGGCGCCTCG
CCGATCACCTCGATCAAGTACGCCGGCTCGCCCTGGGAACTGGGCCTGGCGGAAACCCACCAGACCCTGCGC
GGCAACGACCTGCGCGGCAAGGTCCGGGTGCAGACCGATGGCGGCCTGAAGACCGGCCTGGACGTGATCAAG
GCGGCCATCCTCGGCGCCGAGAGCTTCGGCTTCGGCACCGCACCGATGATCGCCCTGGGCTGCAAGTACCTG
CGCATCTGCCACCTGAACAACTGCGCCACCGGCGTGGCGACGCAGAACGACAAGCTGCGCAAGGACCACTTC
ATCGGCACCACCGCGATGGTGATCAACTTCTTCACCTTCATCGCCACGGAAACCCGCGAGTGGCTGGCGCGC
CTCGGCGTGCGCAGCCTGGGCGAACTGATCGGCCGCACCGACCTGCTGGAGATCCTCCCCGGCGAGACCGCC
AAGCAGCAGAACCTGGATCTCGCTCCGTTGCTGGGCAGTGACCTGATCCCGGCCGACAAGCCGCAGTTCTGC
GAGGTGGAGAAAAACCCGCCGTTCGACCAGGGCCTGCTGGCCGAGAAGATGGTCGAGCTGTCGAAGGCCGCC
ATCGAAGGCCTGAGCGGCGGCGAGTACGAGCTGGACATCTGCAACTGCGACCGATCCATCGGCGCGCGGGTA
TCCGGCGAGATCGCCCGCCTGCACGGCAACCAGGGCATGGCCAAGGCACCGGTGGTGTTCCGTTTCAAGGGC
ACCGCGGGACAGAGCTTCGGCGTGTGGAACGCCGGTGGCCTGCACCTGTACCTGGAAGGCGACGCCAACGAC
TACGTAGGCAAGGGCATGACCGGCGGCAAGCTGGTCATCACCCCGCCCAAGGGCAGTCCGTTCAAGACCCAG
GAATCGGCCATCGTCGGCAACACCTGCCTGTACGGCGCCACCGGCGGCAAGCTGTTCGCCGCCGGAACCGCC
GGCGAGCGCTTCGCCGTGCGCAACTCCGGTTCCCATGCGGTGGTGGAAGGTACCGGCGACCACTGCTGCGAA
TACATGACCGGCGGTTTCGTCTGCGTGCTGGGCAAGACCGGCTACAACTTCGGCTCCGGCATGACCGGGGGC
TTCGCCTACGTCCTCGACATGGACAACACTTTCGTCGACCGTGTGAACCACGAGCTGGTGGAAATCCAGCGG
ATCAGCGGCGAGGCGATGGAGGCCTATCGCAGCCACCTGCGCAAGGTCCTGGTCGAGTACGTGAACGAAACG
GCAAGCGAGTGGGGCGCGAACATCCTGGAAAACCTGGATGACTACCTGCGACGGTTCTGGCTGGTGAAACCG
AAGGCGGCCAGCCTCGGTTCGCTGCTGACCAGCACCCGTGCCAACCCGCAATAA

Translation:

MKAGLYHPETFKDNCGFGLIAHMQGEPSHQLLQTAIEALTCMTHRGGINADGKTGDGCGLLIQKPDLFLRAV
AREAFSVDLPEQYAVGMVFFNQDPVKAEAARENMNREIVAAGLELVGWRKVPIDTSVLGRLALERLPQIEQV
FIGGAGLSDQDFAIKLFSARRRSSVANAADSDHYICSFSHKTIIYKGLMMPADLAAFYPDLGDERLQTAICV
FHQRFSTNTLPKWPLAQPFRFLAHNGEINTITGNRNWAQARRTKFTNELIPDLEELGPLVNRVGSDSSSMDN
MLELMVTGGMDLFRGLRMIIPPAWQNVETMDADLRAFYEYNSLHMEPWDGPAGVVLTDGRYAVCLLDRNGLR
PSRWVTTKNGYITLASEIGVWDYKPEDVIAKGRVGPGQILAVDTETGQVLHSDDIDNRLKSRHPYKQWLRQN
ALRIQATLDDDHGVASYDADQLKQFMKMFQVTFEERDQVLRPLGEQGQEAVGSMGDDTPMAILSRRIRSPYD
YFRQQFAQVTNPPIDPLREAIVMSLETCLGVEQNIFEEAPHHANQAILTTPVISPAKWRTIMTLDRPGFDRH
FIDLNYDESMGLEAAVRNIADQAEEAVRGGKVLLILSDRHIAPGKLPVHAALAVGAVHHRLVQTGLRCDSNI
LVETATARDPHHFAVLIGFGASAVYPFLAYEVLADLIRTGEVLGDLYEVFKYYRKGISKGLMKILSKMGIST
VASYRGAQLFEAVGLSDEVTGLCFTGVASRIQGARFVDIENEQKLLAAEAWSNRKPIQQGGLLKFVYGGEYH
AYNPDVVNTLQAAVQQGDYEKFKEYTALVDQRPVSMIRDLLQVKTAAQPLALDEVEPLEAIFKRFDAAGISL
GALSPEAHEALAEAMNTLGGRSNSGEGGEDPARYGTLKSSKIKQVATGRFGVTPEYLVNAEVLQIKVAQGAK
PGEGGQLPGGKVNGLIARLRYAVPGVTLISPPPHHDIYSIEDLAQLIFDLKQVNPQALVSVKLVSEPGVGTI
AAGVAKAYADLITISGYDGGTGASPITSIKYAGSPWELGLAETHQTLRGNDLRGKVRVQTDGGLKTGLDVIK
AAILGAESFGFGTAPMIALGCKYLRICHLNNCATGVATQNDKLRKDHFIGTTAMVINFFTFIATETREWLAR
LGVRSLGELIGRTDLLEILPGETAKQQNLDLAPLLGSDLIPADKPQFCEVEKNPPFDQGLLAEKMVELSKAA
IEGLSGGEYELDICNCDRSIGARVSGEIARLHGNQGMAKAPVVFRFKGTAGQSFGVWNAGGLHLYLEGDAND
YVGKGMTGGKLVITPPKGSPFKTQESAIVGNTCLYGATGGKLFAAGTAGERFAVRNSGSHAVVEGTGDHCCE
YMTGGFVCVLGKTGYNFGSGMTGGFAYVLDMDNTFVDRVNHELVEIQRISGEAMEAYRSHLRKVLVEYVNET
ASEWGANILENLDDYLRRFWLVKPKAASLGSLLTSTRANPQ*

AnnotationID:22 GeneID:12

AnnotatorUID: diggins

Modification Date/Time: 2005-04-26 10:10:06

Gene Name: gltB

Confidence Code: 2

GeneProduct: glutamate synthase large chain precursor

Cell Localization: ()

Synonyms:

Cell Localization Confidence Code: 5

MolecularFunction:

Functional Category: (2) Amino acid biosynthesis and metabolism

Alternate Gene Product Name:

Functional Category Confidence Code: 5

COGs: pfam04897,COG0069,cd00713,pfam01645,COG0067

Secondary Functional Category(ies):

EC Number: 1.4.1.13

Status: ACTIVE

Pathway:

Homology:

>gb|AAN70641.1|AE016708_2 (AE016793) glutamate synthase, large subunit [Pseudomonas putida
            KT2440]
          Length = 1481

 Score = 2667 bits (6914), Expect = 0.0
 Identities = 1300/1481 (87%), Positives = 1406/1481 (94%)

>gb|AAO58550.1| (AE016874) glutamate synthase, large subunit [Pseudomonas syringae
            pv. tomato str. DC3000]
          Length = 1481

 Score = 2662 bits (6899), Expect = 0.0
 Identities = 1304/1482 (87%), Positives = 1400/1482 (94%), Gaps = 2/1482 (0%)

>gb|AAO09072.1|AE016798_232 (AE016798) Glutamate synthase, large subunit [Vibrio vulnificus
            CMCP6]
          Length = 1487

 Score = 1842 bits (4771), Expect = 0.0
 Identities = 919/1474 (62%), Positives = 1134/1474 (76%), Gaps = 9/1474 (0%)

Structural Features:

pfam04897, Glu_synth_NTN, Glutamate synthase amidotransferase domain.
CD-Length = 431 residues, 100.0% aligned
Score =  637 bits (1646), Expect = 0.0
Identities = 231/431 (53%), Positives = 297/431 (68%), Gaps = 5/431 (1%)

COG0069, GltB, Glutamate synthase domain 2 [Amino acid transport and metabolism]
CD-Length = 485 residues,  99.8% aligned
Score =  641 bits (1654), Expect = 0.0
Identities = 286/485 (58%), Positives = 341/485 (70%), Gaps = 5/485 (1%)

cd00713, Glut_synth, Glutamine amidotransferases class-II (Gn-AT) 
glutamate synthase (Glts)-type.
CD-Length = 410 residues, 100.0% aligned
Score =  515 bits (1328), Expect = 1e-146
Identities = 194/411 (47%), Positives = 266/411 (64%), Gaps = 6/411 (1%)

pfam01645, Glu_synthase, Conserved region in glutamate synthase.
CD-Length = 368 residues, 100.0% aligned
Score =  500 bits (1288), Expect = 6e-142
Identities = 222/368 (60%), Positives = 266/368 (72%), Gaps = 3/368 (0%)

COG0067, GltB, Glutamate synthase domain 1 [Amino acid transport and metabolism]
CD-Length = 371 residues,  99.7% aligned
Score =  479 bits (1233), Expect = 1e-135
Identities = 193/378 (51%), Positives = 244/378 (64%), Gaps = 9/378 (2%)

Genomic Context:

Comment:

ReferenceID:27

Author/Investigator(s): GenNotator

Title:

PubMed:

MedLine:

Source:

Reference Type: BLASTP

Data:

URL:

ReferenceID:17169

Author/Investigator(s): GenNotator

Title: CDD Search

PubMed:

MedLine:

Source:

Reference Type: RPSBLAST

Data:

URL:

ReferenceID:35038

Author/Investigator(s):

Title: CDD Search

PubMed:

MedLine:

Source:

Reference Type: Misc (text/plain)

Data: 12_cdd.html

URL:

ReferenceID:35855

Author/Investigator(s): Pelanda R, Vanoni MA, Perego M, Piubelli L, Galizzi A, Curti B, Zanetti G.

Title: Glutamate synthase genes of the diazotroph Azospirillum brasilense. Cloning, sequencing, and analysis of functional domains.

PubMed: 8428988

MedLine:

Source:

J Biol Chem. 1993 Feb 15;268(5):3099-106.

Reference Type: Journal

Data:

URL:	`http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8428988`

Homologs By Global Alignment

Gene ID:12

Identity:

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

HomologID Accession Description Length PctIdentity PctSimilarity Gaps Score

23 PA5036_tr translation of PA5036 1481 99.86 99.93 0 7682.0

8437 gb|AAG08421.1|AE004916_10 (AE004916) glutamate synthase large chain precursor [Pseudomonas aeruginosa PAO1] 1481 99.86 99.93 0 7682.0

8438 gb|AAB39259.1| (U81261) glutamate synthase large subunit [Pseudomonas aeruginosa] 1483 99.66 99.73 3 7656.0

8440 gb|AAO58550.1| (AE016874) glutamate synthase, large subunit [Pseudomonas syringae pv. tomato str. DC3000] 1482 87.98 94.46 2 6903.0

8439 gb|AAN70641.1|AE016708_2 (AE016793) glutamate synthase, large subunit [Pseudomonas putida KT2440] 1481 87.77 94.93 0 6914.0

GC ORFID: `43014`	How Found: `BLASTX`
GC_TrimmedSeqID: `69`	Blast Result ID
Subject Sequence Name: `t_PA5036`	Glimmer Score:
Start: `5207226`	Stop: `5202781`
Length: `4446`
Start Codon: `ATG`	Truncated Start:
Stop Codon: `TAA`	Truncated Stop:
Homolog: `t_PA5036 translation of PA5036`	Homolog Bit Score `2963.0`
Other Homologs: `psyr_15may02_Scaffold2_revised_gene3298 (ORF: BLASTX 2665.0), t_PA5036 (ORF: GLIMMER)`
GC ORF Sequence
ATGAAAGCAGGTCTGTACCATCCTGAAACGTTCAAGGATAACTGCGGATTTGGCCTGATCGCCCATATGCAG GGCGAGCCGAGCCATCAACTTCTGCAAACCGCAATCGAAGCACTGACCTGCATGACCCACCGCGGTGGGATC AATGCCGATGGCAAGACCGGGGACGGCTGCGGGCTGCTGATCCAGAAGCCCGACCTGTTCCTGCGTGCCGTC GCCAGGGAAGCCTTTTCTGTCGACCTGCCCGAGCAATACGCGGTGGGCATGGTGTTCTTCAACCAGGACCCG GTGAAAGCCGAGGCGGCACGCGAGAACATGAATCGCGAGATCGTCGCCGCCGGACTGGAGCTGGTCGGCTGG CGCAAGGTGCCGATCGATACCAGCGTTCTCGGCCGCCTGGCGCTGGAGCGCCTGCCGCAGATCGAACAGGTG TTCATCGGCGGCGCCGGCCTGAGCGACCAGGACTTCGCCATCAAGCTGTTCAGCGCACGGCGCCGCTCGTCG GTGGCCAACGCCGCCGACAGCGACCACTACATCTGCAGCTTTTCGCACAAGACCATCATCTATAAGGGCCTG ATGATGCCGGCCGACCTGGCCGCCTTCTATCCGGACCTTGGCGACGAGCGCCTGCAGACCGCGATCTGCGTA TTCCACCAGCGCTTCTCCACCAACACCCTGCCGAAATGGCCCCTGGCGCAGCCTTTCCGCTTCCTCGCCCAC AACGGCGAGATCAACACCATCACCGGCAACCGCAACTGGGCCCAGGCGCGGCGGACCAAGTTCACCAACGAA CTGATCCCCGACCTGGAAGAGCTAGGCCCGCTGGTGAACCGCGTCGGCTCCGACTCGTCGAGCATGGACAAC ATGCTCGAACTGATGGTCACCGGCGGCATGGACCTGTTCCGCGGCCTGCGCATGATCATCCCGCCGGCCTGG CAGAACGTCGAGACCATGGACGCCGACCTGCGCGCGTTCTACGAGTATAACTCGCTGCACATGGAGCCCTGG GATGGCCCCGCCGGCGTCGTGCTGACCGACGGTCGCTATGCCGTCTGCCTGCTCGACCGCAATGGCCTGCGC CCGTCGCGCTGGGTCACCACGAAGAACGGCTACATCACCCTGGCCTCGGAGATCGGCGTCTGGGACTACAAG CCCGAGGACGTCATCGCCAAGGGTCGCGTCGGTCCGGGGCAGATCCTCGCGGTGGACACCGAGACCGGCCAG GTCCTGCACAGCGACGACATCGACAACCGCCTGAAGTCGCGCCATCCCTACAAGCAATGGCTGCGGCAGAAT GCCCTGCGCATCCAGGCGACCCTGGACGACGACCATGGCGTGGCCAGCTACGACGCCGACCAGCTCAAGCAA TTCATGAAGATGTTCCAGGTCACCTTCGAGGAGCGTGACCAGGTGCTGCGTCCGCTCGGCGAGCAGGGCCAG GAAGCGGTGGGCTCCATGGGCGACGATACGCCGATGGCGATCCTCTCCCGGCGCATCCGCTCTCCGTACGAT TACTTCCGCCAGCAATTCGCCCAGGTCACCAACCCGCCGATCGACCCGCTGCGCGAAGCCATCGTGATGTCC CTGGAAACCTGCCTGGGCGTCGAGCAGAACATCTTCGAGGAAGCCCCGCACCATGCCAACCAGGCGATCCTC ACCACTCCGGTGATCTCGCCGGCGAAGTGGCGGACCATCATGACCCTGGACCGCCCGGGCTTCGACCGCCAC TTCATCGACCTCAACTACGACGAGTCGATGGGCCTGGAGGCCGCGGTGCGCAACATCGCCGACCAGGCCGAG GAAGCCGTGCGCGGCGGCAAGGTGCTGCTGATCCTCAGCGACCGCCATATCGCTCCCGGCAAACTGCCGGTG CATGCTGCCCTGGCCGTCGGCGCGGTGCATCACCGCCTGGTGCAGACCGGGCTGCGCTGCGACTCCAACATC CTGGTGGAGACCGCCACCGCCCGCGATCCGCACCATTTCGCGGTGCTGATCGGCTTCGGCGCCTCGGCGGTC TATCCGTTCCTCGCCTACGAGGTGCTGGCCGACCTGATCCGCACCGGTGAAGTGCTGGGCGACCTCTACGAG GTGTTCAAGTACTACCGCAAGGGGATCTCCAAGGGCCTGATGAAGATTCTCTCGAAGATGGGCATCTCCACC GTCGCGTCCTACCGTGGCGCGCAGCTGTTCGAGGCGGTGGGCCTGTCCGACGAGGTCACCGGCCTGTGCTTC ACCGGAGTCGCCAGCCGCATCCAGGGTGCGCGCTTCGTCGATATCGAGAACGAGCAGAAGCTCCTGGCCGCC GAGGCCTGGAGCAACCGCAAGCCGATCCAGCAGGGCGGCTTGCTGAAGTTCGTCTATGGCGGCGAATACCAC GCCTACAACCCGGATGTGGTGAATACCCTGCAGGCTGCCGTGCAGCAGGGCGACTACGAGAAGTTCAAGGAA TACACCGCGCTGGTGGACCAGCGCCCGGTGTCGATGATCCGCGACCTGTTGCAGGTGAAGACCGCCGCGCAG CCGCTGGCGCTCGACGAGGTGGAGCCGCTGGAAGCCATCTTCAAGCGCTTCGATGCCGCCGGGATTTCCCTC GGCGCGCTGTCGCCGGAGGCCCACGAGGCGCTGGCCGAGGCGATGAACACCCTGGGCGGTCGTTCCAACTCC GGCGAGGGCGGCGAGGACCCGGCGCGCTACGGCACGCTGAAGAGTTCGAAGATCAAGCAGGTGGCTACCGGC CGCTTCGGCGTGACCCCGGAATACCTGGTCAACGCCGAGGTCCTGCAGATCAAGgTGGCCCAGGGCGCCAAG CCCGGCGAGGGCGGCCAGTTGCCGGGCGGCAAGGTCAACGGCCTGATCGCCCGCCTGCGCTATGCGGTGCCC GGCGTGACCCTGATCTCGCCGCCGCCGCACCACGACATCTACTCCATCGAAGACCTGGCGCAGCTGATCTTC GACCTCAAGCAGGTCAACCCGCAGGCGCTGGTATCGGTGAAGCTGGTATCCGAGCCTGGCGTCGGCACCATC GCCGCCGGCGTGGCCAAGGCCTATGCGGACTTGATCACCATCTCCGGCTACGACGGCGGTACCGGCGCCTCG CCGATCACCTCGATCAAGTACGCCGGCTCGCCCTGGGAACTGGGCCTGGCGGAAACCCACCAGACCCTGCGC GGCAACGACCTGCGCGGCAAGGTCCGGGTGCAGACCGATGGCGGCCTGAAGACCGGCCTGGACGTGATCAAG GCGGCCATCCTCGGCGCCGAGAGCTTCGGCTTCGGCACCGCACCGATGATCGCCCTGGGCTGCAAGTACCTG CGCATCTGCCACCTGAACAACTGCGCCACCGGCGTGGCGACGCAGAACGACAAGCTGCGCAAGGACCACTTC ATCGGCACCACCGCGATGGTGATCAACTTCTTCACCTTCATCGCCACGGAAACCCGCGAGTGGCTGGCGCGC CTCGGCGTGCGCAGCCTGGGCGAACTGATCGGCCGCACCGACCTGCTGGAGATCCTCCCCGGCGAGACCGCC AAGCAGCAGAACCTGGATCTCGCTCCGTTGCTGGGCAGTGACCTGATCCCGGCCGACAAGCCGCAGTTCTGC GAGGTGGAGAAAAACCCGCCGTTCGACCAGGGCCTGCTGGCCGAGAAGATGGTCGAGCTGTCGAAGGCCGCC ATCGAAGGCCTGAGCGGCGGCGAGTACGAGCTGGACATCTGCAACTGCGACCGATCCATCGGCGCGCGGGTA TCCGGCGAGATCGCCCGCCTGCACGGCAACCAGGGCATGGCCAAGGCACCGGTGGTGTTCCGTTTCAAGGGC ACCGCGGGACAGAGCTTCGGCGTGTGGAACGCCGGTGGCCTGCACCTGTACCTGGAAGGCGACGCCAACGAC TACGTAGGCAAGGGCATGACCGGCGGCAAGCTGGTCATCACCCCGCCCAAGGGCAGTCCGTTCAAGACCCAG GAATCGGCCATCGTCGGCAACACCTGCCTGTACGGCGCCACCGGCGGCAAGCTGTTCGCCGCCGGAACCGCC GGCGAGCGCTTCGCCGTGCGCAACTCCGGTTCCCATGCGGTGGTGGAAGGTACCGGCGACCACTGCTGCGAA TACATGACCGGCGGTTTCGTCTGCGTGCTGGGCAAGACCGGCTACAACTTCGGCTCCGGCATGACCGGGGGC TTCGCCTACGTCCTCGACATGGACAACACTTTCGTCGACCGTGTGAACCACGAGCTGGTGGAAATCCAGCGG ATCAGCGGCGAGGCGATGGAGGCCTATCGCAGCCACCTGCGCAAGGTCCTGGTCGAGTACGTGAACGAAACG GCAAGCGAGTGGGGCGCGAACATCCTGGAAAACCTGGATGACTACCTGCGACGGTTCTGGCTGGTGAAACCG AAGGCGGCCAGCCTCGGTTCGCTGCTGACCAGCACCCGTGCCAACCCGCAATAA