ViewGene
Locus Name:
GeneID:
Location:

GeneID:1198
TrivialName: PA14_72380
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_72380
PAO1 Ortholog Locus:PA5483
Sequence Length:1350
Protein Length:449
Start: 6446259
Stop: 6447608
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08868.1|AE004961_3 (AE004961) two-component response regulator AlgB [Pseudomonas aeruginosa PAO1]
Identities = 448/449 (99%), Positives = 448/449 (99%)
Sequence:
ATGGAAACCACTTCCGAAAAACAGGGGCGCATCCTGCTGGTCGATGACGAGTCGGCGATCCTGCGCACTTTC
CGTTATTGCCTCGAAGACGAAGGCTACAGCGTGGCCACCGCCAGCAGCGCGCCGCAGGCGGAGGCCCTGTTG
CAGCGCCAGGTATTCGACCTGTGCTTCCTCGACCTGCGCCTGGGCGAAGACAACGGGCTCGACGTTCTCGCC
CAGATGCGCGTCCAGGCGCCATGGATGCGCGTGGTGATCGTCACCGCGCATTCGGCGGTGGATACCGCGGTC
GACGCCATGCAGGCCGGCGCGGTGGATTACCTGGTCAAGCCCTGCAGCCCGGACCAACTGCGCCTAGCCGCC
GCCAAGCAACTGGAGGTGCGCCAACTGACCGCGCGCCTGGAGGCCCTGGAGGACGAAGTGCGCCGCCAGGGC
GACGGCCTGGAATCGCACAGCCCGGCCATGGCCGCGGTACTGGAGACCGCGCGCCAGGTCGCGGCGACCGAC
GCCAACATCCTCATCCTCGGCGAATCCGGCTCCGGCAAGGGCGAACTGGCACGCGCCATCCACACCTGGAGC
AAACGCGCGAAGAAGCCCCAGGTCACCATCAACTGCCCGTCGCTGACCGCCGAACTGATGGAAAGCGAACTG
TTCGGGCACAGTCGCGGCGCCTTCACCGGTGCCACCGAAAGCACCCTGGGCAGGGTCAGCCAGGCTGACGGC
GGCACCCTGTTCCTCGACGAGATCGGCGACTTCCCGCTGACCTTGCAACCCAAGCTGCTGCGCTTCATCCAG
GACAAGGAATACGAACGCGTCGGCGATCCGGTGACCCGCCGCGCCGATGTGCGCATCCTTGCCGCGACCAAC
CGCGACCTGGGCGCGATGGTCGCCCAGGGCCAGTTCCGCGAGGACCTGCTCTACCGCCTCAACGTGATCGTG
CTCAACCTGCCTCCCCTGCGCGAACGCGCCGAGGATATCCTCGGCCTGGCCGAACGTTTCCTCGCCCGCTTC
GTCAAGGACTACGGCCGCCCCGCCCGCGGCTTCAGCGAAGCCGCCCGCGAGGCCATGCGGCAATACCCCTGG
CCGGGCAACGTACGCGAGCTACGCAACGTGATCGAGCGCGCCAGCATCATCTGCAACCAGGAACGGGTGGAT
GTCGACCACCTCGGTTTCAGCGCTGCGCAATCTGCCAGCAGCGCGCCGCGGATCGGCGAATCGCTGAGCCTG
GAAGACCTGGAGAAAGCCCATATCACGGCGGTGATGGCCTCCAGCGCGACCCTCGACCAGGCCGCCAAGACC
CTCGGTATCGATGCCTCGACCCTGTACCGGAAGCGCAAGCAGTACGGCCTATGA
Translation:
METTSEKQGRILLVDDESAILRTFRYCLEDEGYSVATASSAPQAEALLQRQVFDLCFLDLRLGEDNGLDVLA
QMRVQAPWMRVVIVTAHSAVDTAVDAMQAGAVDYLVKPCSPDQLRLAAAKQLEVRQLTARLEALEDEVRRQG
DGLESHSPAMAAVLETARQVAATDANILILGESGSGKGELARAIHTWSKRAKKPQVTINCPSLTAELMESEL
FGHSRGAFTGATESTLGRVSQADGGTLFLDEIGDFPLTLQPKLLRFIQDKEYERVGDPVTRRADVRILAATN
RDLGAMVAQGQFREDLLYRLNVIVLNLPPLRERAEDILGLAERFLARFVKDYGRPARGFSEAAREAMRQYPW
PGNVRELRNVIERASIICNQERVDVDHLGFSAAQSASSAPRIGESLSLEDLEKAHITAVMASSATLDQAAKT
LGIDASTLYRKRKQYGL*
AnnotationID:1207GeneID:1198
AnnotatorUID: diggins
Modification Date/Time: 2005-03-21 07:07:15
Gene Name:algB
Confidence Code:1
GeneProduct:two-component response regulator AlgB
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(28) Two-component regulatory systems
Alternate Gene Product Name:positive regulator of alginate biosynthetic gene
Functional Category Confidence Code:5
COGs:COG2204,pfam00158
Secondary Functional Category(ies):transcriptional regulator
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO53879.1| (AE016857) alginate biosynthesis transcriptional regulatory protein
           AlgB [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 448

 Score =  737 bits (1902), Expect = 0.0
 Identities = 379/449 (84%), Positives = 410/449 (91%), Gaps = 1/449 (0%)

>gb|AAN65767.1|AE016202_10 (AE016774) alginate biosynthesis transcriptional regulatory protein
           AlgB [Pseudomonas putida KT2440]
          Length = 448

 Score =  732 bits (1889), Expect = 0.0
 Identities = 374/449 (83%), Positives = 408/449 (90%), Gaps = 1/449 (0%)






 
Structural Features:
COG2204, AtoC, Response regulator containing CheY-like receiver, AAA-type ATPase, 
and DNA-binding domains 
[Signal transduction mechanisms]
CD-Length = 464 residues,  98.3% aligned
Score =  485 bits (1249), Expect = 6e-138
Identities = 207/456 (45%), Positives = 277/456 (60%), Gaps = 11/456 (2%)

pfam00158, Sigma54_activat, Sigma-54 interaction domain.
CD-Length = 222 residues, 100.0% aligned
Score =  311 bits (798), Expect = 1e-85
Identities = 131/222 (59%), Positives = 156/222 (70%)







   
Genomic Context:






   
Comment:


















    ReferenceID:2399




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19541




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33233




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1198_cdd.html


    		




 
URL:

















    ReferenceID:35613




  
Author/Investigator(s):
        Wozniak DJ, Ohman DE.





  
Title:
        Pseudomonas aeruginosa AlgB, a two-component response regulator of the NtrC family, is required for algD transcription.




  
PubMed:
        1899859


MedLine:
        




    
Source:
J Bacteriol. 1991 Feb;173(4):1406-13.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1899859









           
Homologs By Global Alignment 

           

       



     
Gene ID:1198
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2162
                           PA5483_tr
                           translation of PA5483
                           449
                           99.77
                           99.77
                           0
                           2237.0
     

     
     
72203
                           gb|AAG08868.1|AE004961_3
                           (AE004961) two-component response regulator AlgB [Pseudomonas aeruginosa PAO1]
                           449
                           99.77
                           99.77
                           0
                           2237.0
     

     
     
72204
                           gb|AAA25700.1|
                           (M62902) algB [Pseudomonas aeruginosa]
                           449
                           99.77
                           99.77
                           0
                           2237.0
     

     
     
72205
                           gb|AAO53879.1|
                           (AE016857) alginate biosynthesis transcriptional regulatory protein AlgB [Pseudomonas syringae pv. tomato str. DC3000]
                           449
                           84.40
                           91.31
                           1
                           1904.0
     

     
     
72206
                           gb|AAN65767.1|AE016202_10
                           (AE016774) alginate biosynthesis transcriptional regulatory protein AlgB [Pseudomonas putida KT2440]
                           449
                           83.29
                           90.86
                           1
                           1891.0
     

     






      

          
GC ORFID: 44200How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5483Glimmer Score: 

          
Start: 5709948Stop: 5711297

          
Length: 1350


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5483 translation of PA5483Homolog Bit Score 866.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3179 (ORF: BLASTX 737.0), t_PA4726 (ORF: BLASTX 311.0), t_PA1335 (ORF: BLASTX 281.0), t_PA1945 (ORF: BLASTX 234.0), t_PA3932 (ORF: BLASTX 165.0), psyr_15may02_Scaffold2_revised_gene3237 (ORF: BLASTX 162.0), t_PA5483 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGAAACCACTTCCGAAAAACAGGGGCGCATCCTGCTGGTCGATGACGAGTCGGCGATCCTGCGCACTTTC 
CGTTATTGCCTCGAAGACGAAGGCTACAGCGTGGCCACCGCCAGCAGCGCGCCGCAGGCGGAGGCCCTGTTG 
CAGCGCCAGGTATTCGACCTGTGCTTCCTCGACCTGCGCCTGGGCGAAGACAACGGGCTCGACGTTCTCGCC 
CAGATGCGCGTCCAGGCGCCATGGATGCGCGTGGTGATCGTCACCGCGCATTCGGCGGTGGATACCGCGGTC 
GACGCCATGCAGGCCGGCGCGGTGGATTACCTGGTCAAGCCCTGCAGCCCGGACCAACTGCGCCTAGCCGCC 
GCCAAGCAACTGGAGGTGCGCCAACTGACCGCGCGCCTGGAGGCCCTGGAGGACGAAGTGCGCCGCCAGGGC 
GACGGCCTGGAATCGCACAGCCCGGCCATGGCCGCGGTACTGGAGACCGCGCGCCAGGTCGCGGCGACCGAC 
GCCAACATCCTCATCCTCGGCGAATCCGGCTCCGGCAAGGGCGAACTGGCACGCGCCATCCACACCTGGAGC 
AAACGCGCGAAGAAGCCCCAGGTCACCATCAACTGCCCGTCGCTGACCGCCGAACTGATGGAAAGCGAACTG 
TTCGGGCACAGTCGCGGCGCCTTCACCGGTGCCACCGAAAGCACCCTGGGCAGGGTCAGCCAGGCTGACGGC 
GGCACCCTGTTCCTCGACGAGATCGGCGACTTCCCGCTGACCTTGCAACCCAAGCTGCTGCGCTTCATCCAG 
GACAAGGAATACGAACGCGTCGGCGATCCGGTGACCCGCCGCGCCGATGTGCGCATCCTTGCCGCGACCAAC 
CGCGACCTGGGCGCGATGGTCGCCCAGGGCCAGTTCCGCGAGGACCTGCTCTACCGCCTCAACGTGATCGTG 
CTCAACCTGCCTCCCCTGCGCGAACGCGCCGAGGATATCCTCGGCCTGGCCGAACGTTTCCTCGCCCGCTTC 
GTCAAGGACTACGGCCGCCCCGCCCGCGGCTTCAGCGAAGCCGCCCGCGAGGCCATGCGGCAATACCCCTGG 
CCGGGCAACGTACGCGAGCTACGCAACGTGATCGAGCGCGCCAGCATCATCTGCAACCAGGAACGGGTGGAT 
GTCGACCACCTCGGTTTCAGCGCTGCGCAATCTGCCAGCAGCGCGCCGCGGATCGGCGAATCGCTGAGCCTG 
GAAGACCTGGAGAAAGCCCATATCACGGCGGTGATGGCCTCCAGCGCGACCCTCGACCAGGCCGCCAAGACC 
CTCGGTATCGATGCCTCGACCCTGTACCGGAAGCGCAAGCAGTACGGCCTATGA