ViewGene
Locus Name:
GeneID:
Location:

GeneID:1196
TrivialName: PA14_63270
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_63270
PAO1 Ortholog Locus:PA4786
Sequence Length:1356
Protein Length:451
Start: 5645892
Stop: 5647247
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08172.1|AE004892_3 (AE004892) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 446/451 (98%), Positives = 449/451 (99%)
Sequence:
ATGACCGATCGTTACATCGCCTTCGCCAACTCCCCCGTCGGCCGCCGCCTGGTTGGCGCCGTCGGCCTTCCC
TCCCCGCTGCGCCTGGAGCGCTGGCAGGCCGGCCGGGTGCGGCCGGTGGACGGCCCGCTGGTGATCGGCGGG
AGCGGCGCGCTGGCCGAGGCCATGCTGCCCTTCGCCGGCAAGCTGACCGACGCAGTGTTCGCCGCCGTCGAG
GGACAGTTCGAGCTGCCGCGCTGGACCGCCGAGCACAGCCCACGGGCCAAGGCGGTGCTGTTCGACGCCAGC
GGCCTGACCCGCTTCGAGCAGTTGGTGGCGCTGCGCGACTTCTTCCAGCCGGTGCTCAAGGGCCTCGACAAG
TGCCCGAAGGTGGTGGTCCTCGGACGCGCCCCCGAGTCTCTCAAGGACCCGGTCGCCGCCAGCGTGCAACGC
AGCCTGGAAGGTTTCACCCGCTCCCTGGGCAAGGAAATCCGTCGCGGCGGCAACGTGCAGTTGCTGTACGTC
GGCAAGGGCGCCGAGGACCAACTGGAAGGCGCACTGCGCTTCTTCCTCTCGCCGAAGAGCGCCTACGTCTCC
GGCCAGGTGATCCGTCTTGCCGCCAACAGCGCCCAGGTCCACGACTGGAGCAAGCCGCTGGCCGGCCAGCGC
GCGCTGGTCACCGGCGCCGCCCGCGGCATTGGCGCGGCCATCGCGGAAACCCTGGCGCGCGACGGCGCGGAA
GTGGTGCTGCTCGACGTGCCGCCGGCCAGGGAGGCCCTCGAAGGCCTCGCCGCGCGCCTCGGCGGCCGTGCC
GTGGCTCTCGACATCTGCGCCGCGGACGCCGGGCAGCAACTGGTGGACGCGCTGCCTGAGGGCGTCGATATC
GTCGTGCACAACGCCGGGATCACTCGCGACAAGACCCTGGCGAAGATGAGCAGCGACTTCTGGAACTCGGTG
ATCAACGTCAACCTCAATGCCCCGCAGGTGCTGACCCAGGCCCTGCTCGACGGCGGCAAGCTGCATGACAAC
GGCCGGGTGGTGTTGCTGGCGTCGATCAGCGGCATCGCCGGCAACCTCGGGCAGAGCAACTACGCGGTGAGC
AAGGCCGGCCTGATCGGCCTGGCCCAGGCCTGGGCGCCGGCGCTGGGCAAGCGCGGCATCACCATCAACGCG
GTGGCGCCGGGGTTCATCGAGACGCAGATGACCGCCGCCATTCCGCTGACCATCCGCGAGGCCGGCCGGCGG
ATGAACTCCATGAGCCAGGGCGGCCTGCCGCAGGACGTCGCCGAAACGGTGGCCTGGTTCGCCCAGCCGTCG
TCCGGCGCGGTCAGCGGCCAGGTGCTGAGGGTGTGCGGGCAGAGCCTGCTGGGGGCCTGA
Translation:
MTDRYIAFANSPVGRRLVGAVGLPSPLRLERWQAGRVRPVDGPLVIGGSGALAEAMLPFAGKLTDAVFAAVE
GQFELPRWTAEHSPRAKAVLFDASGLTRFEQLVALRDFFQPVLKGLDKCPKVVVLGRAPESLKDPVAASVQR
SLEGFTRSLGKEIRRGGNVQLLYVGKGAEDQLEGALRFFLSPKSAYVSGQVIRLAANSAQVHDWSKPLAGQR
ALVTGAARGIGAAIAETLARDGAEVVLLDVPPAREALEGLAARLGGRAVALDICAADAGQQLVDALPEGVDI
VVHNAGITRDKTLAKMSSDFWNSVINVNLNAPQVLTQALLDGGKLHDNGRVVLLASISGIAGNLGQSNYAVS
KAGLIGLAQAWAPALGKRGITINAVAPGFIETQMTAAIPLTIREAGRRMNSMSQGGLPQDVAETVAWFAQPS
SGAVSGQVLRVCGQSLLGA*
AnnotationID:1205GeneID:1196
AnnotatorUID: diggins
Modification Date/Time: 2005-03-10 08:08:18
Gene Name:
Confidence Code:3
GeneProduct:putative short-chain dehydrogenase
Cell Localization:()
Synonyms:PA4786
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00106,COG1028,COG0300,COG4221
Secondary Functional Category(ies):secondary metabolites, biosynthesis, transport, and catabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO54285.1| (AE016858) oxidoreductase, short chain dehydrogenase/reductase
           family [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 451

 Score =  590 bits (1521), Expect = e-168
 Identities = 296/450 (65%), Positives = 366/450 (81%), Gaps = 1/450 (0%)

>gb|AAN66208.1|AE016248_11 (AE016776) 3-oxoacyl-(acyl-carrier-protein) reductase [Pseudomonas
           putida KT2440]
          Length = 457

 Score =  588 bits (1517), Expect = e-167
 Identities = 295/451 (65%), Positives = 364/451 (80%), Gaps = 1/451 (0%)

>emb|CAD14064.1| (AL646059) PROBABLE SHORT-CHAIN DEHYDROGENASE TRANSMEMBRANE PROTEIN
           [Ralstonia solanacearum]
          Length = 470

 Score =  439 bits (1130), Expect = e-122
 Identities = 248/471 (52%), Positives = 312/471 (66%), Gaps = 21/471 (4%)






 
Structural Features:
pfam00106, adh_short, short chain dehydrogenase. 
CD-Length = 247 residues,  99.2% aligned
Score =  159 bits (404), Expect = 6e-40
Identities = 89/245 (36%), Positives = 136/245 (55%), Gaps = 16/245 (6%)

COG1028, FabG, Dehydrogenases with different specificities 
(related to short-chain alcohol dehydrogenases) 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 251 residues,  98.0% aligned
Score =  149 bits (377), Expect = 6e-37
Identities = 104/249 (41%), Positives = 148/249 (59%), Gaps = 18/249 (7%)

COG0300, DltE, Short-chain dehydrogenases of various substrate specificities
CD-Length = 265 residues,  81.9% aligned
Score =  116 bits (291), Expect = 7e-27
Identities = 65/223 (29%), Positives = 109/223 (48%), Gaps = 15/223 (6%)

COG4221, COG4221, Short-chain alcohol dehydrogenase of unknown specificity
CD-Length = 246 residues,  91.1% aligned
Score =  110 bits (277), Expect = 3e-25
Identities = 76/230 (33%), Positives = 117/230 (50%), Gaps = 17/230 (7%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2395




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19537




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37699




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1196_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:1196
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2161
                           PA4786_tr
                           translation of PA4786
                           451
                           98.89
                           99.55
                           0
                           2240.0
     

     
     
72151
                           gb|AAG08172.1|AE004892_3
                           (AE004892) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
                           451
                           98.89
                           99.55
                           0
                           2240.0
     

     
     
72152
                           gb|AAO54285.1|
                           (AE016858) oxidoreductase, short chain dehydrogenase/reductase family [Pseudomonas syringae pv. tomato str. DC3000]
                           455
                           65.49
                           81.09
                           8
                           1523.0
     

     
     
72153
                           gb|AAN66208.1|AE016248_11
                           (AE016776) 3-oxoacyl-(acyl-carrier-protein) reductase [Pseudomonas putida KT2440]
                           458
                           64.41
                           79.47
                           8
                           1519.0
     

     
     
72155
                           emb|CAB77292.1|
                           (AL939120) putative bifunctional protein (ketoreductase/3-oxoacyl-[acyl-carrier protein] reductase) [Streptomyces coelicolor A3(2)]
                           454
                           52.86
                           65.85
                           27
                           1084.0
     

     






      

          
GC ORFID: 44198How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4786Glimmer Score: 

          
Start: 4909572Stop: 4910927

          
Length: 1356


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4786 translation of PA4786Homolog Bit Score 867.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3612 (ORF: BLASTX 584.0), t_PA4786 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCGATCGTTACATCGCCTTCGCCAACTCCCCCGTCGGCCGCCGCCTGGTTGGCGCCGTCGGCCTTCCC 
TCCCCGCTGCGCCTGGAGCGCTGGCAGGCCGGCCGGGTGCGGCCGGTGGACGGCCCGCTGGTGATCGGCGGG 
AGCGGCGCGCTGGCCGAGGCCATGCTGCCCTTCGCCGGCAAGCTGACCGACGCAGTGTTCGCCGCCGTCGAG 
GGACAGTTCGAGCTGCCGCGCTGGACCGCCGAGCACAGCCCACGGGCCAAGGCGGTGCTGTTCGACGCCAGC 
GGCCTGACCCGCTTCGAGCAGTTGGTGGCGCTGCGCGACTTCTTCCAGCCGGTGCTCAAGGGCCTCGACAAG 
TGCCCGAAGGTGGTGGTCCTCGGACGCGCCCCCGAGTCTCTCAAGGACCCGGTCGCCGCCAGCGTGCAACGC 
AGCCTGGAAGGTTTCACCCGCTCCCTGGGCAAGGAAATCCGTCGCGGCGGCAACGTGCAGTTGCTGTACGTC 
GGCAAGGGCGCCGAGGACCAACTGGAAGGCGCACTGCGCTTCTTCCTCTCGCCGAAGAGCGCCTACGTCTCC 
GGCCAGGTGATCCGTCTTGCCGCCAACAGCGCCCAGGTCCACGACTGGAGCAAGCCGCTGGCCGGCCAGCGC 
GCGCTGGTCACCGGCGCCGCCCGCGGCATTGGCGCGGCCATCGCGGAAACCCTGGCGCGCGACGGCGCGGAA 
GTGGTGCTGCTCGACGTGCCGCCGGCCAGGGAGGCCCTCGAAGGCCTCGCCGCGCGCCTCGGCGGCCGTGCC 
GTGGCTCTCGACATCTGCGCCGCGGACGCCGGGCAGCAACTGGTGGACGCGCTGCCTGAGGGCGTCGATATC 
GTCGTGCACAACGCCGGGATCACTCGCGACAAGACCCTGGCGAAGATGAGCAGCGACTTCTGGAACTCGGTG 
ATCAACGTCAACCTCAATGCCCCGCAGGTGCTGACCCAGGCCCTGCTCGACGGCGGCAAGCTGCATGACAAC 
GGCCGGGTGGTGTTGCTGGCGTCGATCAGCGGCATCGCCGGCAACCTCGGGCAGAGCAACTACGCGGTGAGC 
AAGGCCGGCCTGATCGGCCTGGCCCAGGCCTGGGCGCCGGCGCTGGGCAAGCGCGGCATCACCATCAACGCG 
GTGGCGCCGGGGTTCATCGAGACGCAGATGACCGCCGCCATTCCGCTGACCATCCGCGAGGCCGGCCGGCGG 
ATGAACTCCATGAGCCAGGGCGGCCTGCCGCAGGACGTCGCCGAAACGGTGGCCTGGTTCGCCCAGCCGTCG 
TCCGGCGCGGTCAGCGGCCAGGTGCTGAGGGTGTGCGGGCAGAGCCTGCTGGGGGCCTGA