ViewGene
Locus Name:
GeneID:
Location:

GeneID:1183
TrivialName: PA14_68680
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_68680
PAO1 Ortholog Locus:PA5199
Sequence Length:1320
Protein Length:439
Start: 6124768
Stop: 6123449
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08584.1|AE004932_11 (AE004932) two-component sensor EnvZ [Pseudomonas aeruginosa PAO1]
Identities = 438/439 (99%), Positives = 439/439 (100%)
Sequence:
ATGAAAACGCCGCTCTGGTTCCCGCAAAGCTTCTTCGCCCGCACCCTCTGGCTGGTGCTGATCGTCGTGCTG
TTCTCCAAGGCGCTGACCCTGGTCTACCTGCTGATGAACGAGGACGTGATCGTCGACCGCCAGTACAGCCAC
GGCGCGGCGCTGACCATCCGCGCGTTCTGGGCTGCCGACGAGGAGTCGCGCGCGGCCATCGCCAAGGCTTCC
GGCCTGCGCTGGGTGCCGAGCAGCGCCGACCAGCCCGGCGAGCAGCACTGGCCGTACACGGAAATCTTCCAG
CGGCAGATGCAGATGGAGCTGGGGCCGGACACCGAGACCCGCCTGCGTATCCACCAGCCGTCGCAGCTCTGG
GTGCGCGCACCGAGCCTCGGCGAAGGCTGGCTGGCGGTGCCGCTGTATCCGCATCCCTTGCGTGGCCAACGG
ATCTGGAGCGTGCTCGGCTGGTTCCTCGGCATCGGCCTGCTCTCCACCGCCGCCGCCTGGATCTTCGTCCGC
CAGCTCAGCCAGCCGCTCAAGCGCCTGGTGGTCGCCGCCCGCCAGTTCGGCCAGGGGCGCAGCGTGCGCCTG
CCGCTGGGGCCGGAGACGCCAAGCGAAATGGCCGAGGTGTATCGCGCCTTCAACCAGATGGCCGAGGACATC
GAGCAGGGCGGGCGCGAACGCGAATTGATGTTGGCCGGTGTCTCCCACGACCTGCGCACCCCGCTGACACGC
CTGCGCCTGTCGCTGGAGTTGCTGCCGGAGAGCGAACGGGAGATGGTCGAGGACATGGTCCGCGACATCGAG
GACATGGATGCGATCCTCGACCAGTTCCTCGCCTTCATCCGCGACGGCCGCGACGAGCCGGTGGAGGAGGGC
GACCTGACCGACCTGGTGCGCGAGGTGGTGGCACCGTTCAACCAGACTCGCGAGCAGGTGCGCATGGCCTTG
CAGCCGGTACCGGCGATGCCGTTGCGGCGGGTGTCGATGAAGCGTCTGCTGGGCAACCTGATCGACAATGCC
CTGAACCACGGTGGCGGCTCGGTTGAGGTGGCCAGCTACGTGGCCGGCGAAAGCGCGGCGCCCTACGTGGTG
CTGAGTGTGCTCGATCGCGGCCAGGGCATCGATCCCGCGGAAGTGGACAGCATCTTCAATCCCTTCATCCGC
GGCGACAAGGCGCGTGGCGGCAAGGGCACCGGCCTCGGCCTGGCGATCGTCAAGCGCATCGCCGCCCAGCAC
GGCGGCAGCGTCGAACTGCGCAACCGCGATGGCGGCGGCCTGGAAGCGCGGGTCTGCCTGCCGCTGGGGTTG
CTGCTACCGCGCGGCGCGGCCTGA
Translation:
MKTPLWFPQSFFARTLWLVLIVVLFSKALTLVYLLMNEDVIVDRQYSHGAALTIRAFWAADEESRAAIAKAS
GLRWVPSSADQPGEQHWPYTEIFQRQMQMELGPDTETRLRIHQPSQLWVRAPSLGEGWLAVPLYPHPLRGQR
IWSVLGWFLGIGLLSTAAAWIFVRQLSQPLKRLVVAARQFGQGRSVRLPLGPETPSEMAEVYRAFNQMAEDI
EQGGRERELMLAGVSHDLRTPLTRLRLSLELLPESEREMVEDMVRDIEDMDAILDQFLAFIRDGRDEPVEEG
DLTDLVREVVAPFNQTREQVRMALQPVPAMPLRRVSMKRLLGNLIDNALNHGGGSVEVASYVAGESAAPYVV
LSVLDRGQGIDPAEVDSIFNPFIRGDKARGGKGTGLGLAIVKRIAAQHGGSVELRNRDGGGLEARVCLPLGL
LLPRGAA*
AnnotationID:1192GeneID:1183
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 13:01:14
Gene Name:envZ
Confidence Code:2
GeneProduct:two-component sensor EnvZ
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(28) Two-component regulatory systems
Alternate Gene Product Name:signal transduction histidine kinase
Functional Category Confidence Code:5
COGs:COG0642,smart00387,cd00075
Secondary Functional Category(ies):signal transduction mechanism
EC Number:2.7.3.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN65879.1|AE016215_14 (AE016774) osmolarity sensor protein EnvZ [Pseudomonas putida
           KT2440]
          Length = 437

 Score =  703 bits (1814), Expect = 0.0
 Identities = 351/438 (80%), Positives = 389/438 (88%), Gaps = 2/438 (0%

>gb|AAO53874.1| (AE016857) osmolarity sensor protein EnvZ [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 440

 Score =  686 bits (1771), Expect = 0.0
 Identities = 343/438 (78%), Positives = 380/438 (86%), Gaps = 3/438 (0%)








 
Structural Features:
COG0642, BaeS, Signal transduction histidine kinase [Signal transduction mechanisms]
CD-Length = 336 residues,  97.3% aligned
Score =  145 bits (366), Expect = 8e-36
Identities = 97/335 (28%), Positives = 154/335 (45%), Gaps = 22/335 (6%)

smart00387, HATPase_c, Histidine kinase-like ATPases
CD-Length = 112 residues,  93.8% aligned
Score = 93.4 bits (232), Expect = 5e-20
Identities = 43/109 (39%), Positives = 66/109 (60%), Gaps = 7/109 (6%)

cd00075, HATPase_c, Histidine kinase-like ATPases
CD-Length = 103 residues, 100.0% aligned
Score = 90.4 bits (224), Expect = 4e-19
Identities = 37/107 (34%), Positives = 62/107 (57%), Gaps = 7/107 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2369




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19511




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34875




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1183_cdd.html


    		




 
URL:

















    ReferenceID:35762




  
Author/Investigator(s):
        Tow LA, Coyne VE.





  
Title:
        Cloning and characterisation of a novel ompB operon from Vibrio cholerae 569B.




  
PubMed:
        10023081


MedLine:
        




    
Source:
Biochim Biophys Acta. 1999 Feb 16;1444(2):269-75.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10023081

















    ReferenceID:35763




  
Author/Investigator(s):
        Tabatabai N, Forst S.





  
Title:
        Molecular analysis of the two-component genes, ompR and envZ, in the symbiotic bacterium Xenorhabdus nematophilus.




  
PubMed:
        8801419


MedLine:
        




    
Source:
Mol Microbiol. 1995 Aug;17(4):643-52.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8801419

















    ReferenceID:35764




  
Author/Investigator(s):
        Mizuno T, Wurtzel ET, Inouye M.





  
Title:
        Osmoregulation of gene expression. II. DNA sequence of the envZ gene of the ompB operon of Escherichia coli and characterization of its gene product.




  
PubMed:
        6292200


MedLine:
        




    
Source:
J Biol Chem. 1982 Nov 25;257(22):13692-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6292200









           
Homologs By Global Alignment 

           

       



     
Gene ID:1183
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2114
                           PA5199_tr
                           translation of PA5199
                           439
                           99.77
                           99.99
                           0
                           2254.0
     

     
     
70748
                           gb|AAG08584.1|AE004932_11
                           (AE004932) two-component sensor EnvZ [Pseudomonas aeruginosa PAO1]
                           439
                           99.77
                           99.99
                           0
                           2254.0
     

     
     
70749
                           gb|AAN65879.1|AE016215_14
                           (AE016774) osmolarity sensor protein EnvZ [Pseudomonas putida KT2440]
                           439
                           79.95
                           88.61
                           2
                           1818.0
     

     
     
70750
                           gb|AAO53874.1|
                           (AE016857) osmolarity sensor protein EnvZ [Pseudomonas syringae pv. tomato str. DC3000]
                           443
                           77.42
                           85.77
                           7
                           1774.0
     

     






      

          
GC ORFID: 44185How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5199Glimmer Score: 

          
Start: 5388454Stop: 5387135

          
Length: 1320


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5199 translation of PA5199Homolog Bit Score 872.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3176 (ORF: BLASTX 681.0), t_PA5199 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAAACGCCGCTCTGGTTCCCGCAAAGCTTCTTCGCCCGCACCCTCTGGCTGGTGCTGATCGTCGTGCTG 
TTCTCCAAGGCGCTGACCCTGGTCTACCTGCTGATGAACGAGGACGTGATCGTCGACCGCCAGTACAGCCAC 
GGCGCGGCGCTGACCATCCGCGCGTTCTGGGCTGCCGACGAGGAGTCGCGCGCGGCCATCGCCAAGGCTTCC 
GGCCTGCGCTGGGTGCCGAGCAGCGCCGACCAGCCCGGCGAGCAGCACTGGCCGTACACGGAAATCTTCCAG 
CGGCAGATGCAGATGGAGCTGGGGCCGGACACCGAGACCCGCCTGCGTATCCACCAGCCGTCGCAGCTCTGG 
GTGCGCGCACCGAGCCTCGGCGAAGGCTGGCTGGCGGTGCCGCTGTATCCGCATCCCTTGCGTGGCCAACGG 
ATCTGGAGCGTGCTCGGCTGGTTCCTCGGCATCGGCCTGCTCTCCACCGCCGCCGCCTGGATCTTCGTCCGC 
CAGCTCAGCCAGCCGCTCAAGCGCCTGGTGGTCGCCGCCCGCCAGTTCGGCCAGGGGCGCAGCGTGCGCCTG 
CCGCTGGGGCCGGAGACGCCAAGCGAAATGGCCGAGGTGTATCGCGCCTTCAACCAGATGGCCGAGGACATC 
GAGCAGGGCGGGCGCGAACGCGAATTGATGTTGGCCGGTGTCTCCCACGACCTGCGCACCCCGCTGACACGC 
CTGCGCCTGTCGCTGGAGTTGCTGCCGGAGAGCGAACGGGAGATGGTCGAGGACATGGTCCGCGACATCGAG 
GACATGGATGCGATCCTCGACCAGTTCCTCGCCTTCATCCGCGACGGCCGCGACGAGCCGGTGGAGGAGGGC 
GACCTGACCGACCTGGTGCGCGAGGTGGTGGCACCGTTCAACCAGACTCGCGAGCAGGTGCGCATGGCCTTG 
CAGCCGGTACCGGCGATGCCGTTGCGGCGGGTGTCGATGAAGCGTCTGCTGGGCAACCTGATCGACAATGCC 
CTGAACCACGGTGGCGGCTCGGTTGAGGTGGCCAGCTACGTGGCCGGCGAAAGCGCGGCGCCCTACGTGGTG 
CTGAGTGTGCTCGATCGCGGCCAGGGCATCGATCCCGCGGAAGTGGACAGCATCTTCAATCCCTTCATCCGC 
GGCGACAAGGCGCGTGGCGGCAAGGGCACCGGCCTCGGCCTGGCGATCGTCAAGCGCATCGCCGCCCAGCAC 
GGCGGCAGCGTCGAACTGCGCAACCGCGATGGCGGCGGCCTGGAAGCGCGGGTCTGCCTGCCGCTGGGGTTG 
CTGCTACCGCGCGGCGCGGCCTGA