ViewGene
Locus Name:
GeneID:
Location:

GeneID:1182
TrivialName: PA14_57370
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_57370
PAO1 Ortholog Locus:PA4414
Sequence Length:1347
Protein Length:448
Start: 5112234
Stop: 5110888
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07802.1|AE004856_13 (AE004856) UDP-N-acetylmuramoylalanine--D-glutamate ligase [Pseudomonas aeruginosa PAO1]
Identities = 447/448 (99%), Positives = 447/448 (99%)
Sequence:
ATGAGCCTGATCGCCTCCGACCACTTCCGCATCGTTGTCGGCCTCGGCAAGAGCGGCATGTCCCTGGTGCGC
TACCTGGCGCGCCGCGGCTTGCCTTTCGCCGTGGTCGATACCCGAGAGAACCCGCCGGAGCTGGCCACCCTG
CGTGCCCAGTATCCGCAGGTGGAAGTGCGTTGCGGCGAACTCGACGCCGAGTTCCTCTGCTCCGCCCGCGAA
CTCTACGTCAGCCCCGGCTTGTCGCTGCGCACCCCTGCGCTGGTACAGGCCGCCGCGAAAGGCGTGCGCATC
TCCGGTGACATCGATCTCTTCGCCCGCGAGGCGAAGGCACCGATCGTCGCCATCACCGGTTCCAACGCGAAG
AGCACCGTGACCACCCTGGTGGGCGAAATGGCGGTGGCCGCGGACAAGCGTGTCGCCGTCGGCGGCAACCTC
GGCACCCCGGCGCTCGACCTGCTGGCCGACGACATCGAGCTGTACGTGCTGGAGCTGTCGAGCTTCCAGCTG
GAAACCTGCGATCGCCTCAACGCCGAGGTGGCGACCGTGCTGAACGTCAGCGAAGACCATATGGATCGCTAC
GACGGCATGGCTGACTACCACCTGGCCAAGCACCGGATCTTCCGCGGTGCCCGCCAGGTCGTGGTGAATCGC
GCCGATGCCCTGACCCGACCGCTGATCGCCGATACCGTGCCGTGCTGGTCGTTCGACCTGAACAAGCCGGAC
TTCAAGGCTTTCGGCCTGATCGAGGAAGACGGCCAGAAGTGGCTGGCGTTCCAGTTCGACAAGCTGCTGCCG
GTCGGCGAACTGAAGATCCGTGGCGCCCACAACTATTCCAACGCGCTCGCCGCGCTGGCGCTGGGCCATGCG
GTCGGCCTGCCGTTCGACGCCATGCTCGGCGCGCTGAAGGCGTTTTCCGGCCTGGCTCATCGCTGCCAGTGG
GTACGCGAGCGGCAGGGCGTGAGCTACTACGACGATTCCAAGGCCACCAACGTCGGCGCCGCCCTGGCGGCG
ATCGAGGGGCTGGGTGCCGACATCGACGGCAAGCTGGTGCTGCTCGCCGGCGGAGACGGCAAGGGCGCCGAT
TTCCATGACCTGCGCGAGCCGGTCGCGCGCTTCTGCCGGGCGGTGGTACTGCTTGGCCGTGACGCCGGGCTG
ATTGCCCAGGCACTGGGCAACGCGGTACCGCTGGTGCGCGTCGCAACGCTGGACGAAGCAGTCCGGCAGGCC
GCCGAGCTGGCCCGCGAAGGCGATGCGGTGCTGTTGTCGCCGGCCTGCGCGAGCCTGGACATGTTCAAGAAC
TTCGAAGAACGCGGACGCCTGTTCGCCAAAGCCGTAGAGGAGCTAGCGTGA
Translation:
MSLIASDHFRIVVGLGKSGMSLVRYLARRGLPFAVVDTRENPPELATLRAQYPQVEVRCGELDAEFLCSARE
LYVSPGLSLRTPALVQAAAKGVRISGDIDLFAREAKAPIVAITGSNAKSTVTTLVGEMAVAADKRVAVGGNL
GTPALDLLADDIELYVLELSSFQLETCDRLNAEVATVLNVSEDHMDRYDGMADYHLAKHRIFRGARQVVVNR
ADALTRPLIADTVPCWSFDLNKPDFKAFGLIEEDGQKWLAFQFDKLLPVGELKIRGAHNYSNALAALALGHA
VGLPFDAMLGALKAFSGLAHRCQWVRERQGVSYYDDSKATNVGAALAAIEGLGADIDGKLVLLAGGDGKGAD
FHDLREPVARFCRAVVLLGRDAGLIAQALGNAVPLVRVATLDEAVRQAAELAREGDAVLLSPACASLDMFKN
FEERGRLFAKAVEELA*
AnnotationID:1191GeneID:1182
AnnotatorUID: diggins
Modification Date/Time: 2005-05-02 18:06:06
Gene Name:murD
Confidence Code:2
GeneProduct:UDP-N-acetylmuramoylalanine-D-glutamate ligase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0771,pfam01225
Secondary Functional Category(ies):
EC Number:6.3.2.9
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66958.1|AE016324_8 (AE016779) UDP-N-acetylmuramoylalanine--D-glutamate ligase
           [Pseudomonas putida KT2440]
          Length = 450

 Score =  708 bits (1828), Expect = 0.0
 Identities = 361/448 (80%), Positives = 396/448 (88%)


>gb|AAO57859.1| (AE016871) UDP-N-acetylmuramoylalanine--D-glutamate ligase
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 448

 Score =  707 bits (1826), Expect = 0.0
 Identities = 353/448 (78%), Positives = 399/448 (89%)






 
Structural Features:
COG0771, MurD, UDP-N-acetylmuramoylalanine-D-glutamate ligase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 448 residues,  97.3% aligned
Score =  451 bits (1162), Expect = 2e-127
Identities = 215/446 (48%), Positives = 275/446 (61%), Gaps = 19/446 (4%)

pfam01225, Mur_ligase, Mur ligase family, catalytic domain.
CD-Length = 267 residues,  88.4% aligned
Score =  117 bits (293), Expect = 7e-27
Identities = 61/239 (25%), Positives = 102/239 (42%), Gaps = 19/239 (7%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2367




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19509




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:29625




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1182_cdd.html


    		




 
URL:

















    ReferenceID:32800




  
Author/Investigator(s):
        Azzolina BA, Yuan X, Anderson MS, El-Sherbeini M.





  
Title:
        The cell wall and cell division gene cluster in the Mra operon of Pseudomonas aeruginosa: cloning, production, and purification of active enzymes.




  
PubMed:
        11281713


MedLine:
        




    
Source:
Protein Expr Purif. 2001 Apr;21(3):393-400.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11281713









           
Homologs By Global Alignment 

           

       



     
Gene ID:1182
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2113
                           PA4414_tr
                           translation of PA4414
                           448
                           99.77
                           99.77
                           0
                           2253.0
     

     
     
70712
                           gb|AAG07802.1|AE004856_13
                           (AE004856) UDP-N-acetylmuramoylalanine--D-glutamate ligase [Pseudomonas aeruginosa PAO1]
                           448
                           99.77
                           99.77
                           0
                           2253.0
     

     
     
70713
                           gb|AAG45237.1|
                           (AY008276) MurD [Pseudomonas aeruginosa]
                           448
                           99.33
                           99.33
                           0
                           2242.0
     

     
     
70714
                           gb|AAN66958.1|AE016324_8
                           (AE016779) UDP-N-acetylmuramoylalanine--D-glutamate ligase [Pseudomonas putida KT2440]
                           450
                           80.22
                           88.00
                           2
                           1828.0
     

     
     
70715
                           gb|AAO57859.1|
                           (AE016871) UDP-N-acetylmuramoylalanine--D-glutamate ligase [Pseudomonas syringae pv. tomato str. DC3000]
                           448
                           78.79
                           89.06
                           0
                           1826.0
     

     






      

          
GC ORFID: 44184How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4414Glimmer Score: 

          
Start: 4375912Stop: 4374566

          
Length: 1347


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4414 translation of PA4414Homolog Bit Score 872.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1126 (ORF: BLASTX 699.0), t_PA4414 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCTGATCGCCTCCGACCACTTCCGCATCGTTGTCGGCCTCGGCAAGAGCGGCATGTCCCTGGTGCGC 
TACCTGGCGCGCCGCGGCTTGCCTTTCGCCGTGGTCGATACCCGAGAGAACCCGCCGGAGCTGGCCACCCTG 
CGTGCCCAGTATCCGCAGGTGGAAGTGCGTTGCGGCGAACTCGACGCCGAGTTCCTCTGCTCCGCCCGCGAA 
CTCTACGTCAGCCCCGGCTTGTCGCTGCGCACCCCTGCGCTGGTACAGGCCGCCGCGAAAGGCGTGCGCATC 
TCCGGTGACATCGATCTCTTCGCCCGCGAGGCGAAGGCACCGATCGTCGCCATCACCGGTTCCAACGCGAAG 
AGCACCGTGACCACCCTGGTGGGCGAAATGGCGGTGGCCGCGGACAAGCGTGTCGCCGTCGGCGGCAACCTC 
GGCACCCCGGCGCTCGACCTGCTGGCCGACGACATCGAGCTGTACGTGCTGGAGCTGTCGAGCTTCCAGCTG 
GAAACCTGCGATCGCCTCAACGCCGAGGTGGCGACCGTGCTGAACGTCAGCGAAGACCATATGGATCGCTAC 
GACGGCATGGCTGACTACCACCTGGCCAAGCACCGGATCTTCCGCGGTGCCCGCCAGGTCGTGGTGAATCGC 
GCCGATGCCCTGACCCGACCGCTGATCGCCGATACCGTGCCGTGCTGGTCGTTCGACCTGAACAAGCCGGAC 
TTCAAGGCTTTCGGCCTGATCGAGGAAGACGGCCAGAAGTGGCTGGCGTTCCAGTTCGACAAGCTGCTGCCG 
GTCGGCGAACTGAAGATCCGTGGCGCCCACAACTATTCCAACGCGCTCGCCGCGCTGGCGCTGGGCCATGCG 
GTCGGCCTGCCGTTCGACGCCATGCTCGGCGCGCTGAAGGCGTTTTCCGGCCTGGCTCATCGCTGCCAGTGG 
GTACGCGAGCGGCAGGGCGTGAGCTACTACGACGATTCCAAGGCCACCAACGTCGGCGCCGCCCTGGCGGCG 
ATCGAGGGGCTGGGTGCCGACATCGACGGCAAGCTGGTGCTGCTCGCCGGCGGAGACGGCAAGGGCGCCGAT 
TTCCATGACCTGCGCGAGCCGGTCGCGCGCTTCTGCCGGGCGGTGGTACTGCTTGGCCGTGACGCCGGGCTG 
ATTGCCCAGGCACTGGGCAACGCGGTACCGCTGGTGCGCGTCGCAACGCTGGACGAAGCAGTCCGGCAGGCC 
GCCGAGCTGGCCCGCGAAGGCGATGCGGTGCTGTTGTCGCCGGCCTGCGCGAGCCTGGACATGTTCAAGAAC 
TTCGAAGAACGCGGACGCCTGTTCGCCAAAGCCGTAGAGGAGCTAGCGTGA