ViewGene
Locus Name:
GeneID:
Location:

GeneID:1178
TrivialName: PA14_59270
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_59270
PAO1 Ortholog Locus:
Sequence Length:1326
Protein Length:441
Start: 5280280
Stop: 5281605
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAP84209.1| (AY273869) PilO2 [Pseudomonas aeruginosa]
Identities = 441/441 (100%), Positives = 441/441 (100%)
Sequence:
ATGGAGAAGCCTGACCTCGGCAGCCGTGGACCAGACGTCTCGATCCTGAGCTACCACGGCAACAAGTTCGTC
AGCGGCCTGTTCTGGCGGCCGCTGTCCAGCCAGCGGCAGTACATGAAGGAAGCGCGCAAGCTGGGCAAGGAA
GAGCATCTGGACATCGTTGCCATCCGCCATTCACCGACGGTGATCCAGGCCGGCTTCGTTTCGAAGTCGCAA
GGCGCAGTCAAGGGGATGTACTCCCTGGCCTCGGCGCTTTCAGGCCAGTTCGACGGCGACTTCCTGGCCTGC
TGGAAAGTCGACGAGGACCGCTACGCGCTGGTCGCCACGCTCGATGGCGCGATTGTCCCCGGGCAGGATCTG
GTCACCACCCTCGACGAGGCCCGGGACCGGGTCAGGAAGCTCTCTACGCGCGGCGTGCTGCGAAACGCACAG
GTCTTCGTTCCCGAAGGGTTCGATTTCCCCGTCAAGGACTTCGACATCGAGGAACTGCTCGCGCCGAAGCGC
CTGCGGCGCGACTACCGCCTCCGGCAACTCACCTTCGGCTTGTCCGCCAGGGAGTGGACGGCAGTGGCCCTG
CTCGGTTGCGTGGTAGGTGGGTCGCTAACCGCCTACTACCTATGGAATGCCCACCAGGAAGAGCTCGCCAGG
CAAGCCGCGCTCCTCGAGGAGCAGAGGCGCCTCGCCGAGCTGGCCGAGAAGAACGCCCAGGCCAAGCAGCCG
CTGGACCTGGCGTCATTGCAGAAGCCTTGGACGCTCATACCTGACCTCGAGGACATGCTACGCGCCTGTAGC
AAGGCAACGGGGGTACTGTCGCTGTCGATCCAGGGCTGGCTCTTCGAATCCAGCAAGTGCGACGGCAGGGTC
CTGGTCGCCACCTACCACCGTACCGGCAACAGCACAGCAGCCGACCTGACAGCGGCCAGCCAGCACCTGTTC
GCCGACCGCCCCGCCTTCGTCATCGACAACGGCAACACCGCGGCCCTGAAGGTCGATCTGAAGGTTGCCATC
GGCAGTGATGAGCCGCTACTGCCGGCGGACGACGTTCTGCAGGCGCTGACGAGCCACCTGTACCGTCAAGGG
GTCGAGCCCAAGCTGTCGATCAGCCaGGAGACAACTCCGCCCCTCCCTGGCGCGGAAGCTGCGACTGAACAG
CAAGTGGTGTTGCCTTCCTGGAAGAAATTCACCTTCAGCGCCCagACCCGGCTCCCGGCAGACCTGACCTTC
CAGGGGCTGCCCGCTGCCGGTGTCCGCATCACCAACCTCGAAACCACGCTcAAGGACAGCCAGTTGGACTGG
ACTGTCACAGGAGAAATCTATGCGAACTGA
Translation:
MEKPDLGSRGPDVSILSYHGNKFVSGLFWRPLSSQRQYMKEARKLGKEEHLDIVAIRHSPTVIQAGFVSKSQ
GAVKGMYSLASALSGQFDGDFLACWKVDEDRYALVATLDGAIVPGQDLVTTLDEARDRVRKLSTRGVLRNAQ
VFVPEGFDFPVKDFDIEELLAPKRLRRDYRLRQLTFGLSAREWTAVALLGCVVGGSLTAYYLWNAHQEELAR
QAALLEEQRRLAELAEKNAQAKQPLDLASLQKPWTLIPDLEDMLRACSKATGVLSLSIQGWLFESSKCDGRV
LVATYHRTGNSTAADLTAASQHLFADRPAFVIDNGNTAALKVDLKVAIGSDEPLLPADDVLQALTSHLYRQG
VEPKLSISQETTPPLPGAEAATEQQVVLPSWKKFTFSAQTRLPADLTFQGLPAAGVRITNLETTLKDSQLDW
TVTGEIYAN*
AnnotationID:1187GeneID:1178
AnnotatorUID: diggins
Modification Date/Time: 2006-01-31 06:06:19
Gene Name:pilO2
Confidence Code:2
GeneProduct:type IV b pilus protein
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(16) Motility & Attachment
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam06864
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAL18830.2| (AY258503) PilO [Escherichia coli]
          Length = 431

 Score = 84.3 bits (207), Expect = 1e-15
 Identities = 103/459 (22%), Positives = 189/459 (41%), Gaps = 54/459 (11%)

>gb|AAO71702.1| (AE016848) putative pilus assembly protein [Salmonella enterica
           subsp. enterica serovar Typhi Ty2]
          Length = 431

 Score = 84.0 bits (206), Expect = 2e-15
 Identities = 95/436 (21%), Positives = 175/436 (40%), Gaps = 27/436 (6%)






 
Structural Features:
pfam06864, PAP_PilO, Pilin accessory protein (PilO). 
CD-Length = 419 residues,  99.5% aligned
Score =  127 bits (320), Expect = 2e-30
Identities = 97/437 (22%), Positives = 171/437 (39%), Gaps = 39/437 (8%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2359




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19501




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:38295




  
Author/Investigator(s):
        Sakai D, Komano T.





  
Title:
        Genes required for plasmid R64 thin-pilus biogenesis: identification and localization of products of the pilK, pilM, pilO, pilP, pilR, and pilT genes.




  
PubMed:
        11751821


MedLine:
        




    
Source:
J Bacteriol. 2002 Jan;184(2):444-51.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=11751821

















    ReferenceID:38296




  
Author/Investigator(s):
        Castric P.





  
Title:
        pilO, a gene required for glycosylation of Pseudomonas aeruginosa 1244 pilin.




  
PubMed:
        7773418


MedLine:
        




    
Source:
Microbiology. 1995 May;141 ( Pt 5):1247-54.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7773418&query_hl=2&itool=pubmed_docsum

















    ReferenceID:38297




  
Author/Investigator(s):
        Martin PR, Watson AA, McCaul TF, Mattick JS.





  
Title:
        Characterization of a five-gene cluster required for the biogenesis of type 4 fimbriae in Pseudomonas aeruginosa.




  
PubMed:
        7565110


MedLine:
        




    
Source:
Mol Microbiol. 1995 May;16(3):497-508.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7565110&query_hl=2&itool=pubmed_docsum









           
Homologs By Global Alignment 

           

       



     
Gene ID:1178
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
70599
                           gb|AAP84209.1|
                           (AY273869) PilO2 [Pseudomonas aeruginosa]
                           441
                           99.99
                           99.99
                           0
                           2256.0
     

     






      

          
GC ORFID: 44180How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_RL084Glimmer Score: 

          
Start: 4543959Stop: 4545284

          
Length: 1326


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_RL084 pilO 441 Homolog: PilO / Type IVB pilus biosynthesis; Pathogenicity-related / S.enterica subsp. enterica serovar / Dublin (AAF81211) translation of RL084Homolog Bit Score 873.0

          
Other Homologs:  t_RL084 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGAGAAGCCTGACCTCGGCAGCCGTGGACCAGACGTCTCGATCCTGAGCTACCACGGCAACAAGTTCGTC 
AGCGGCCTGTTCTGGCGGCCGCTGTCCAGCCAGCGGCAGTACATGAAGGAAGCGCGCAAGCTGGGCAAGGAA 
GAGCATCTGGACATCGTTGCCATCCGCCATTCACCGACGGTGATCCAGGCCGGCTTCGTTTCGAAGTCGCAA 
GGCGCAGTCAAGGGGATGTACTCCCTGGCCTCGGCGCTTTCAGGCCAGTTCGACGGCGACTTCCTGGCCTGC 
TGGAAAGTCGACGAGGACCGCTACGCGCTGGTCGCCACGCTCGATGGCGCGATTGTCCCCGGGCAGGATCTG 
GTCACCACCCTCGACGAGGCCCGGGACCGGGTCAGGAAGCTCTCTACGCGCGGCGTGCTGCGAAACGCACAG 
GTCTTCGTTCCCGAAGGGTTCGATTTCCCCGTCAAGGACTTCGACATCGAGGAACTGCTCGCGCCGAAGCGC 
CTGCGGCGCGACTACCGCCTCCGGCAACTCACCTTCGGCTTGTCCGCCAGGGAGTGGACGGCAGTGGCCCTG 
CTCGGTTGCGTGGTAGGTGGGTCGCTAACCGCCTACTACCTATGGAATGCCCACCAGGAAGAGCTCGCCAGG 
CAAGCCGCGCTCCTCGAGGAGCAGAGGCGCCTCGCCGAGCTGGCCGAGAAGAACGCCCAGGCCAAGCAGCCG 
CTGGACCTGGCGTCATTGCAGAAGCCTTGGACGCTCATACCTGACCTCGAGGACATGCTACGCGCCTGTAGC 
AAGGCAACGGGGGTACTGTCGCTGTCGATCCAGGGCTGGCTCTTCGAATCCAGCAAGTGCGACGGCAGGGTC 
CTGGTCGCCACCTACCACCGTACCGGCAACAGCACAGCAGCCGACCTGACAGCGGCCAGCCAGCACCTGTTC 
GCCGACCGCCCCGCCTTCGTCATCGACAACGGCAACACCGCGGCCCTGAAGGTCGATCTGAAGGTTGCCATC 
GGCAGTGATGAGCCGCTACTGCCGGCGGACGACGTTCTGCAGGCGCTGACGAGCCACCTGTACCGTCAAGGG 
GTCGAGCCCAAGCTGTCGATCAGCCaGGAGACAACTCCGCCCCTCCCTGGCGCGGAAGCTGCGACTGAACAG 
CAAGTGGTGTTGCCTTCCTGGAAGAAATTCACCTTCAGCGCCCagACCCGGCTCCCGGCAGACCTGACCTTC 
CAGGGGCTGCCCGCTGCCGGTGTCCGCATCACCAACCTCGAAACCACGCTcAAGGACAGCCAGTTGGACTGG 
ACTGTCACAGGAGAAATCTATGCGAACTGA