ViewGene
Locus Name:
GeneID:
Location:

GeneID:1177
TrivialName: PA14_02900
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_02900
PAO1 Ortholog Locus:PA0235
Sequence Length:1347
Protein Length:448
Start: 256110
Stop: 254764
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03624.1|AE004461_7 (AE004461) 4-hydroxybenzoate transporter PcaK [Pseudomonas aeruginosa PAO1]
Identities = 447/448 (99%), Positives = 447/448 (99%)
Sequence:
ATGAACAGCCCCAGCCTGCCCGCCGTCGAACGACTCGACGTGCAAGCCTTCATCAATGCCCAGCCGCTTTCG
CCGTACCAATGGCGGATCGTCCTGCTGTGCTTCCTGATCGTCTTCCTCGATGGCCTCGACACCGCCGCGATG
GGCTTCATCGCGCCGGCGCTGACCCAGGACTGGGGCATCGACCGCGCCAGCCTCGGCCCGGTGATGAGCGCC
GCGCTGATCGGCATGGTCTTCGGCGCCCTCGGCTCCGGACCGCTGGCCGACCGCTACGGACGCAAGCTGGTA
TTGGTCGCGGCGGTCTTCCTCTTCGGCCTGTTCAGCCTGGCCTCGGCCTACAGCACCAACGTCGAGCAACTG
CTGGCGCTGCGCTTCCTCACCGGCCTCGGCCTCGGCGCGGCGATGCCCAACGCCACCACCCTGCTTTCCGAA
TACACCCCCGAGCGCCTCAAGTCGCTGCTGGTGACCAGCATGTTCTGTGGCTTCAACCTGGGCATGGCCTGC
GGCGGATTCGTCTCGGCGAAGCTGATCCCGCTGTTCGGCTGGCACAGCCTGCTGCTGCTCGGCGGCCTGCTA
CCGCTGGTCCTGGCGGTAGTCCTGCTGTTCCGCCTGCCGGAATCGGCGCGCTACCTGGTGGTGCGCAACCGC
GGCAGCGAGCGGGTACGCCAGGTGCTGGCCCCCATCGCCCCGGCGCAGGTGGCGCTGGCACGGAGTTTCCAC
GTCCCCGAGCAGCAGACGGTGCAGGCCCGCAACGTGTTCGCGGTGATCTTTTCCGGTACCTACAGCGCCGGC
ACCCTGCTGCTCTGGCTGACCTACTTCATGGGCCTGGTGATCGTCTACCTGCTCACCAGCTGGCTGCCGACC
CTGATGCGCGATAGCGGCGCGAGCCTGGAGCACGCGGCGTTCATCGGCGCGCTGTTCCAGTTCGGCGGGGTG
CTCAGCGCCGTAGCGGTGGGCTGGGCGATGGATCGCTTCAACCCGCACAAGGTGATCGGCCTGTTCTACCTG
CTGGCGGGGGTGTTCGCCTGGTGCGTCGGCCAGAGCCTCGGCCAGGTCACCCTACTGGCAACCCTGGTGCTG
CTGGCCGGCATGTGCATCAACGGCGCGCAGTCGGCGATGCCGTCGCTGGCGGCGCGCTTCTATCCGACCCAG
GGGCGGGCCACAGGGGTCTCCTGGATGCTCGGTATCGGCCGCTTCGGCGCCATCCTCGGCGCCTGGATCGGC
GCCACCCTGCTGGGCCTGGGCTGGAACTTCGAACAGGTGCTGACCGCCCTGGTGCTGCCGGCTGCACTGGCC
ACCGCCGCGGTGCTGCTGAAGGGGTTGGTCAGTCACGCGGATGCGGGGTGA
Translation:
MNSPSLPAVERLDVQAFINAQPLSPYQWRIVLLCFLIVFLDGLDTAAMGFIAPALTQDWGIDRASLGPVMSA
ALIGMVFGALGSGPLADRYGRKLVLVAAVFLFGLFSLASAYSTNVEQLLALRFLTGLGLGAAMPNATTLLSE
YTPERLKSLLVTSMFCGFNLGMACGGFVSAKLIPLFGWHSLLLLGGLLPLVLAVVLLFRLPESARYLVVRNR
GSERVRQVLAPIAPAQVALARSFHVPEQQTVQARNVFAVIFSGTYSAGTLLLWLTYFMGLVIVYLLTSWLPT
LMRDSGASLEHAAFIGALFQFGGVLSAVAVGWAMDRFNPHKVIGLFYLLAGVFAWCVGQSLGQVTLLATLVL
LAGMCINGAQSAMPSLAARFYPTQGRATGVSWMLGIGRFGAILGAWIGATLLGLGWNFEQVLTALVLPAALA
TAAVLLKGLVSHADAG*
AnnotationID:1186GeneID:1177
AnnotatorUID: diggins
Modification Date/Time: 2005-03-23 18:06:14
Gene Name:pcaK
Confidence Code:2
GeneProduct:4-hydroxybenzoate transporter PcaK
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00083,COG2814
Secondary Functional Category(ies):transport of small molecules
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55852.1| (AE016864) 4-hydroxybenzoate transporter [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 449

 Score =  756 bits (1951), Expect = 0.0
 Identities = 381/441 (86%), Positives = 408/441 (92%)

>gb|AAN66999.1|AE016328_4 (AE016779) 4-hydroxybenzoate transporter [Pseudomonas putida
           KT2440]
          Length = 448

 Score =  744 bits (1921), Expect = 0.0
 Identities = 373/447 (83%), Positives = 410/447 (91%)








 
Structural Features:
pfam00083, Sugar_tr, Sugar (and other) transporter.
CD-Length = 448 residues,  90.0% aligned
Score = 97.6 bits (243), Expect = 3e-21
Identities = 87/409 (21%), Positives = 152/409 (37%), Gaps = 35/409 (8%)

COG2814, AraJ, Arabinose efflux permease 
[Carbohydrate transport and metabolism]
CD-Length = 394 residues,  97.7% aligned
Score = 78.3 bits (193), Expect = 2e-15
Identities = 90/422 (21%), Positives = 146/422 (34%), Gaps = 38/422 (9%)







   
Genomic Context:






   
Comment:


















    ReferenceID:2357




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19499




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33590




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1177_cdd.html


    		




 
URL:

















    ReferenceID:35453




  
Author/Investigator(s):
        Harwood CS, Nichols NN, Kim MK, Ditty JL, Parales RE.





  
Title:
        Identification of the pcaRKF gene cluster from Pseudomonas putida: involvement in chemotaxis, biodegradation, and transport of 4-hydroxybenzoate.




  
PubMed:
        7961399


MedLine:
        




    
Source:
J Bacteriol. 1994 Nov;176(21):6479-88.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7961399









           
Homologs By Global Alignment 

           

       



     
Gene ID:1177
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2107
                           PA0235_tr
                           translation of PA0235
                           448
                           99.77
                           99.77
                           0
                           2257.0
     

     
     
70580
                           gb|AAG03624.1|AE004461_7
                           (AE004461) 4-hydroxybenzoate transporter PcaK [Pseudomonas aeruginosa PAO1]
                           448
                           99.77
                           99.77
                           0
                           2257.0
     

     
     
70581
                           gb|AAO55852.1|
                           (AE016864) 4-hydroxybenzoate transporter [Pseudomonas syringae pv. tomato str. DC3000]
                           449
                           85.07
                           91.31
                           1
                           1945.0
     

     
     
70582
                           gb|AAN66999.1|AE016328_4
                           (AE016779) 4-hydroxybenzoate transporter [Pseudomonas putida KT2440]
                           448
                           83.25
                           91.51
                           0
                           1919.0
     

     
     
70583
                           gb|AAA85137.1|
                           (U10895) PcaK [Pseudomonas putida]
                           448
                           82.58
                           91.51
                           0
                           1913.0
     

     






      

          
GC ORFID: 44179How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0235Glimmer Score: 

          
Start: 6057451Stop: 6056105

          
Length: 1347


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0235 translation of PA0235Homolog Bit Score 874.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1096 (ORF: BLASTX 753.0), t_PA2472 (ORF: BLASTX 413.0), t_PA0235 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACAGCCCCAGCCTGCCCGCCGTCGAACGACTCGACGTGCAAGCCTTCATCAATGCCCAGCCGCTTTCG 
CCGTACCAATGGCGGATCGTCCTGCTGTGCTTCCTGATCGTCTTCCTCGATGGCCTCGACACCGCCGCGATG 
GGCTTCATCGCGCCGGCGCTGACCCAGGACTGGGGCATCGACCGCGCCAGCCTCGGCCCGGTGATGAGCGCC 
GCGCTGATCGGCATGGTCTTCGGCGCCCTCGGCTCCGGACCGCTGGCCGACCGCTACGGACGCAAGCTGGTA 
TTGGTCGCGGCGGTCTTCCTCTTCGGCCTGTTCAGCCTGGCCTCGGCCTACAGCACCAACGTCGAGCAACTG 
CTGGCGCTGCGCTTCCTCACCGGCCTCGGCCTCGGCGCGGCGATGCCCAACGCCACCACCCTGCTTTCCGAA 
TACACCCCCGAGCGCCTCAAGTCGCTGCTGGTGACCAGCATGTTCTGTGGCTTCAACCTGGGCATGGCCTGC 
GGCGGATTCGTCTCGGCGAAGCTGATCCCGCTGTTCGGCTGGCACAGCCTGCTGCTGCTCGGCGGCCTGCTA 
CCGCTGGTCCTGGCGGTAGTCCTGCTGTTCCGCCTGCCGGAATCGGCGCGCTACCTGGTGGTGCGCAACCGC 
GGCAGCGAGCGGGTACGCCAGGTGCTGGCCCCCATCGCCCCGGCGCAGGTGGCGCTGGCACGGAGTTTCCAC 
GTCCCCGAGCAGCAGACGGTGCAGGCCCGCAACGTGTTCGCGGTGATCTTTTCCGGTACCTACAGCGCCGGC 
ACCCTGCTGCTCTGGCTGACCTACTTCATGGGCCTGGTGATCGTCTACCTGCTCACCAGCTGGCTGCCGACC 
CTGATGCGCGATAGCGGCGCGAGCCTGGAGCACGCGGCGTTCATCGGCGCGCTGTTCCAGTTCGGCGGGGTG 
CTCAGCGCCGTAGCGGTGGGCTGGGCGATGGATCGCTTCAACCCGCACAAGGTGATCGGCCTGTTCTACCTG 
CTGGCGGGGGTGTTCGCCTGGTGCGTCGGCCAGAGCCTCGGCCAGGTCACCCTACTGGCAACCCTGGTGCTG 
CTGGCCGGCATGTGCATCAACGGCGCGCAGTCGGCGATGCCGTCGCTGGCGGCGCGCTTCTATCCGACCCAG 
GGGCGGGCCACAGGGGTCTCCTGGATGCTCGGTATCGGCCGCTTCGGCGCCATCCTCGGCGCCTGGATCGGC 
GCCACCCTGCTGGGCCTGGGCTGGAACTTCGAACAGGTGCTGACCGCCCTGGTGCTGCCGGCTGCACTGGCC 
ACCGCCGCGGTGCTGCTGAAGGGGTTGGTCAGTCACGCGGATGCGGGGTGA