ViewGene
Locus Name:
GeneID:
Location:

GeneID:1172
TrivialName: PA14_44070
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_44070
PAO1 Ortholog Locus:PA1580
Sequence Length:1287
Protein Length:428
Start: 3923114
Stop: 3924400
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04969.1|AE004586_7 (AE004586) citrate synthase [Pseudomonas aeruginosa PAO1]
Identities = 428/428 (100%), Positives = 428/428 (100%)
Sequence:
ATGGCTGACAAAAAAGCGCAGTTGATCATCGAGGGCTCAGCCCCCGTCGAACTGCCCGTCCTATCCGGTACC
ATGGGTCCCGATGTAGTGGATGTACGGGGCCTCACCGCCACGGGCCACTTCACCTTCGATCCTGGCTTCATG
TCGACCGCCTCCTGCGAGTCGAAGATCACCTATATCGACGGCGACAAAGGCGTCCTCCTCCATCGCGGCTAC
CCCATCGAGCAACTGGCGGAGAAATCCGACTACCTGGAAACCTGCTACCTGCTGCTGAACGGCGAGCTGCCC
ACCGCCGCGCAGAAGGAACAGTTCGTCGGCACCATCAAGAACCACACCATGGTTCACGAGCAGCTGAAGACC
TTCTTCAACGGCTTCCGCCGCGACGCCCATCCGATGGCCGTGATGTGCGGCGTGATCGGCGCCCTCTCGGCC
TTCTACCACGACTCCCTGGACATCAATAACCCGAAGCATCGCGAAGTCTCCGCGCATCGCCTGATCGCCAAG
ATGCCGACCATCGCCGCCATGGTGTACAAGTACTCCAAGGGCGAGCCGATGATGTATCCGCGTAACGACCTG
AACTACGCGGAAAACTTCCTGCACATGATGTTCAACACCCCCTGCGAGACCAAGCCGATCAGCCCCGTGCTG
GCCAAGGCCATGGACCGCATCTTCATTCTCCACGCCGACCACGAGCAGAACGCCTCCACCTCCACGGTGCGT
CTGGCCGGCTCCTCCGGCGCCAATCCGTTCGCCTGCATCGCCTCCGGCATCGCCGCCCTGTGGGGACCGGCC
CATGGCGGCGCGAACGAAGCGGTGCTGCGCATGCTCGACGAGATCGGCGACGTGTCCAACATCGACAAGTTC
GTCGAGAAGGCCAAGGACAAGAACGATCCGTTCAAGCTGATGGGCTTCGGCCATCGTGTCTACAAGAACTTC
GACCCGCGCGCCAAGGTCATGAAGCAGACCTGCGACGAGGTCCTCCAGGAACTGGGCATCAACGACCCGCAA
CTGGAACTGGCGATGAAGCTGGAAGAAATCGCCCGCCACGACCCCTACTTCGTGGAACGCAACCTGTACCCG
AACGTCGACTTCTACTCGGGGATCATCCTCAAGGCGATCGGCATTCCGACCAGCATGTTCACCGTGATCTTC
GCCCTGGCGCGTACCGTCGGCTGGATCTCGCACTGGCAGGAAATGCTCTCCGGTCCCTACAAGATCGGCCGC
CCGCGCCAGCTCTATACCGGCCACACCCAGCGCGACTTCACCGCCCTCAAGGATCGCGGCTGA
Translation:
MADKKAQLIIEGSAPVELPVLSGTMGPDVVDVRGLTATGHFTFDPGFMSTASCESKITYIDGDKGVLLHRGY
PIEQLAEKSDYLETCYLLLNGELPTAAQKEQFVGTIKNHTMVHEQLKTFFNGFRRDAHPMAVMCGVIGALSA
FYHDSLDINNPKHREVSAHRLIAKMPTIAAMVYKYSKGEPMMYPRNDLNYAENFLHMMFNTPCETKPISPVL
AKAMDRIFILHADHEQNASTSTVRLAGSSGANPFACIASGIAALWGPAHGGANEAVLRMLDEIGDVSNIDKF
VEKAKDKNDPFKLMGFGHRVYKNFDPRAKVMKQTCDEVLQELGINDPQLELAMKLEEIARHDPYFVERNLYP
NVDFYSGIILKAIGIPTSMFTVIFALARTVGWISHWQEMLSGPYKIGRPRQLYTGHTQRDFTALKDRG*
AnnotationID:1181GeneID:1172
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 06:06:38
Gene Name:gltA
Confidence Code:1
GeneProduct:citrate synthase
Cell Localization:()
Synonyms:cisY
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00285,COG0372
Secondary Functional Category(ies):
EC Number:4.1.3.7
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69775.1|AE016614_1 (AE016789) citrate synthase [Pseudomonas putida KT2440]
          Length = 429

 Score =  792 bits (2046), Expect = 0.0
 Identities = 382/428 (89%), Positives = 408/428 (95%), Gaps = 1/428 (0%)

>gb|AAO55710.1| (AE016863) citrate synthase I [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 429

 Score =  791 bits (2042), Expect = 0.0
 Identities = 382/428 (89%), Positives = 407/428 (95%), Gaps = 1/428 (0%)

>gb|AAN79269.1|AE016757_173 (AE016757) Citrate synthase [Escherichia coli CFT073]
          Length = 427

 Score =  634 bits (1635), Expect = 0.0
 Identities = 296/421 (70%), Positives = 357/421 (84%)






 
Structural Features:
pfam00285, Citrate_synt, Citrate synthase.
CD-Length = 359 residues, 100.0% aligned
Score =  540 bits (1393), Expect = 1e-154
Identities = 193/365 (52%), Positives = 238/365 (65%), Gaps = 7/365 (1%)

COG0372, GltA, Citrate synthase [Energy production and conversion]
CD-Length = 390 residues, 100.0% aligned
Score =  506 bits (1304), Expect = 2e-144
Identities = 216/402 (53%), Positives = 279/402 (69%), Gaps = 13/402 (3%)








   
Genomic Context:






   
Comment:


















    ReferenceID:2347




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19489




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37244




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1172_cdd.html


    		




 
URL:

















    ReferenceID:37912




  
Author/Investigator(s):
        Donald LJ, Molgat GF, Duckworth HW.





  
Title:
        Cloning, sequencing, and expression of the gene for NADH-sensitive citrate synthase of Pseudomonas aeruginosa.




  
PubMed:
        2507528


MedLine:
        




    
Source:
J Bacteriol. 1989 Oct;171(10):5542-50.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2507528









           
Homologs By Global Alignment 

           

       



     
Gene ID:1172
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2100
                           PA1580_tr
                           translation of PA1580
                           428
                           99.99
                           99.99
                           0
                           2259.0
     

     
     
70102
                           gb|AAG04969.1|AE004586_7
                           (AE004586) citrate synthase [Pseudomonas aeruginosa PAO1]
                           428
                           99.99
                           99.99
                           0
                           2259.0
     

     
     
70103
                           gb|AAA25769.1|
                           (M29728) citrate synthase [Pseudomonas aeruginosa]
                           428
                           99.53
                           99.76
                           0
                           2250.0
     

     
     
70104
                           gb|AAN69775.1|AE016614_1
                           (AE016789) citrate synthase [Pseudomonas putida KT2440]
                           429
                           89.04
                           95.10
                           1
                           2046.0
     

     
     
70105
                           gb|AAO55710.1|
                           (AE016863) citrate synthase I [Pseudomonas syringae pv. tomato str. DC3000]
                           429
                           89.04
                           94.87
                           1
                           2042.0
     

     






      

          
GC ORFID: 44174How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1580Glimmer Score: 

          
Start: 1030795Stop: 1029509

          
Length: 1287


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1580 translation of PA1580Homolog Bit Score 874.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene438 (ORF: BLASTX 788.0), t_PA1580 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCTGACAAAAAAGCGCAGTTGATCATCGAGGGCTCAGCCCCCGTCGAACTGCCCGTCCTATCCGGTACC 
ATGGGTCCCGATGTAGTGGATGTACGGGGCCTCACCGCCACGGGCCACTTCACCTTCGATCCTGGCTTCATG 
TCGACCGCCTCCTGCGAGTCGAAGATCACCTATATCGACGGCGACAAAGGCGTCCTCCTCCATCGCGGCTAC 
CCCATCGAGCAACTGGCGGAGAAATCCGACTACCTGGAAACCTGCTACCTGCTGCTGAACGGCGAGCTGCCC 
ACCGCCGCGCAGAAGGAACAGTTCGTCGGCACCATCAAGAACCACACCATGGTTCACGAGCAGCTGAAGACC 
TTCTTCAACGGCTTCCGCCGCGACGCCCATCCGATGGCCGTGATGTGCGGCGTGATCGGCGCCCTCTCGGCC 
TTCTACCACGACTCCCTGGACATCAATAACCCGAAGCATCGCGAAGTCTCCGCGCATCGCCTGATCGCCAAG 
ATGCCGACCATCGCCGCCATGGTGTACAAGTACTCCAAGGGCGAGCCGATGATGTATCCGCGTAACGACCTG 
AACTACGCGGAAAACTTCCTGCACATGATGTTCAACACCCCCTGCGAGACCAAGCCGATCAGCCCCGTGCTG 
GCCAAGGCCATGGACCGCATCTTCATTCTCCACGCCGACCACGAGCAGAACGCCTCCACCTCCACGGTGCGT 
CTGGCCGGCTCCTCCGGCGCCAATCCGTTCGCCTGCATCGCCTCCGGCATCGCCGCCCTGTGGGGACCGGCC 
CATGGCGGCGCGAACGAAGCGGTGCTGCGCATGCTCGACGAGATCGGCGACGTGTCCAACATCGACAAGTTC 
GTCGAGAAGGCCAAGGACAAGAACGATCCGTTCAAGCTGATGGGCTTCGGCCATCGTGTCTACAAGAACTTC 
GACCCGCGCGCCAAGGTCATGAAGCAGACCTGCGACGAGGTCCTCCAGGAACTGGGCATCAACGACCCGCAA 
CTGGAACTGGCGATGAAGCTGGAAGAAATCGCCCGCCACGACCCCTACTTCGTGGAACGCAACCTGTACCCG 
AACGTCGACTTCTACTCGGGGATCATCCTCAAGGCGATCGGCATTCCGACCAGCATGTTCACCGTGATCTTC 
GCCCTGGCGCGTACCGTCGGCTGGATCTCGCACTGGCAGGAAATGCTCTCCGGTCCCTACAAGATCGGCCGC 
CCGCGCCAGCTCTATACCGGCCACACCCAGCGCGACTTCACCGCCCTCAAGGATCGCGGCTGA