ViewGene
Locus Name:
GeneID:
Location:

GeneID:1171
TrivialName: PA14_25280
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_25280
PAO1 Ortholog Locus:PA2999
Sequence Length:1338
Protein Length:445
Start: 2211845
Stop: 2213182
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06387.1|AE004724_16 (AE004724) Na+-translocating NADH:ubiquinone oxidoreductase subunit Nrq1 [Pseudomonas aeruginosa PAO1]
Identities = 442/445 (99%), Positives = 444/445 (99%)
Sequence:
ATGATCAAGATAAAACGTGGCCTGGACCTGCCCATCAGCGGCGCCCCCGAACAGCGCATCGAGGCAGCACGC
CCGGTACGCAGCGTGGCGTTGATCGGTTTCGACTACCACGGCATGAAGCCGACCATGGCGGTGCAGGTAGGC
GACCGGGTGAAGCTGGGGCAGGCGCTGTTCACCGACAAGAAGAATCCCTCCGTCAGCTATACCGCACCCGGC
GCCGGTGTGGTCAGTGCCATCCATCGTGGCGAGAAGCGCGTCCTGCAATCGGTGGTCATCGATCTCGACGGC
GACGAGCAACTGGAGTTCGCCCGTTATCCCGCGGACCAACTCGCCACGCTGAGCGCCGAGCAGGTCCGCGAC
AACCTGTTGCAGTCGGGCCTGTGGACCGCGCTGCGGACCCGGCCGTTCAGCAAGGTGCCCGATCCCGAGTCC
AGCCCCAGTTCGATTTTCGTCACTGCCATCGATACTCAGCCGCTGGCCGCCGATCCGCAGGTGGTTATCGCG
GAGCAGGGCGAGGCCTTCCAGGCCGGGCTGACGGTGCTCGGGCGCCTGGCCCGGGTGTTCCTGTGCAAGGCG
GAAGGCGTTTCGCTACCCGGCGAGGCGCTCTCCGGCGTGACCGCGCAAGCCTTCGCCGGTCCGCATCCGGCG
GGCCTGCCCGGCACCCATATCCACTTCCTCGACCCGGTGGGTGCCGGCAAGAGCGTGTGGAACCTGAACTAC
CAGGACGTGATCGCCATCGGCAAGCTGTTCACGACCGGGCAATTGTGGACCGAGCGGGTGATCGCCCTGGCC
GGTCCGGTGGTGGAAAAGCCGCGTCTGGTGCGCACCCGGTTGGGCGCCAACCTCGACGAACTGGCCGCCGGC
CAGCTGCAGCCGGGCAACAACCGGCTGATCTCCGGCTCCGTGCTGGGCGGTCGCACCGCCCATGGCGCCTAC
GCCTACCTGGGGCGCTACCACCTGCAGTTGTCCTGTCTCAAGGAGGGCGACCAGCGCGAGTTCCTCCACTAC
CTGCGCGCCGGGGTGGAAAAGCATTCGCTGCTCAACGTGTTCGTCTCCAGGCTGCTCGGCGGCAAGCGTTTC
GCCTTCACCACCAGCACCAACGGCAGCCCGCGTGCGATGGTGCCGGTAGGCAATTACGAGGCGGTGATGCCG
CTGGATATCCTGCCGACCCAACTGCTGCGCTACCTGATCGTCGGCGACACGGAAATGGCCCAGAAGCTCGGC
GCCCTGGAACTCGACGAAGAGGACCTGGCGCTGTGCAGCTACGTCTGCGCCGGCAAGTACGAATACGGCCCG
ATTCTGCGGGACAACCTGGCACGCATCGAGCAGGAGGGCTGA
Translation:
MIKIKRGLDLPISGAPEQRIEAARPVRSVALIGFDYHGMKPTMAVQVGDRVKLGQALFTDKKNPSVSYTAPG
AGVVSAIHRGEKRVLQSVVIDLDGDEQLEFARYPADQLATLSAEQVRDNLLQSGLWTALRTRPFSKVPDPES
SPSSIFVTAIDTQPLAADPQVVIAEQGEAFQAGLTVLGRLARVFLCKAEGVSLPGEALSGVTAQAFAGPHPA
GLPGTHIHFLDPVGAGKSVWNLNYQDVIAIGKLFTTGQLWTERVIALAGPVVEKPRLVRTRLGANLDELAAG
QLQPGNNRLISGSVLGGRTAHGAYAYLGRYHLQLSCLKEGDQREFLHYLRAGVEKHSLLNVFVSRLLGGKRF
AFTTSTNGSPRAMVPVGNYEAVMPLDILPTQLLRYLIVGDTEMAQKLGALELDEEDLALCSYVCAGKYEYGP
ILRDNLARIEQEG*
AnnotationID:1180GeneID:1171
AnnotatorUID: diggins
Modification Date/Time: 2005-04-11 14:02:26
Gene Name:nqrA
Confidence Code:2
GeneProduct:Na+-translocating NADH:ubiquinone oxidoreductase subunit Nrq1
Cell Localization:()
Synonyms:aopA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:Na(+)-translocating NADH-quinone reductase subunit A
Functional Category Confidence Code:5
COGs:pfam05896,COG1726
Secondary Functional Category(ies):
EC Number:1.6.5.-
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAC92475.1| (AJ414156) NADH-ubiquinone oxidoreductase subunit A [Yersinia
           pestis CO92]
          Length = 447

 Score =  530 bits (1366), Expect = e-150
 Identities = 267/447 (59%), Positives = 337/447 (75%), Gaps = 2/447 (0%)

>emb|CAB84035.1| (AL162754) putative Na(+)-translocating NADH-ubiquinone reductase
           subunit A [Neisseria meningitidis Z2491]
          Length = 447

 Score =  525 bits (1353), Expect = e-148
 Identities = 265/447 (59%), Positives = 329/447 (73%), Gaps = 2/447 (0%)

>gb|AAC21836.1| (U32702) NADH:ubiquinone oxidoreducatase subunit A, Na
           translocating [Haemophilus influenzae Rd]
          Length = 447

 Score =  522 bits (1344), Expect = e-147
 Identities = 268/448 (59%), Positives = 335/448 (74%), Gaps = 4/448 (0%)






 
Structural Features:
pfam05896, NQRA, Na(+)-translocating NADH-quinone reductase subunit A (NQRA).
CD-Length = 447 residues, 100.0% aligned
Score =  694 bits (1793), Expect = 0.0
Identities = 292/447 (65%), Positives = 348/447 (77%), Gaps = 2/447 (0%)

COG1726, NqrA, Na+-transporting NADH:ubiquinone oxidoreductase, 
subunit NqrA 
[Energy production and conversion]
CD-Length = 447 residues, 100.0% aligned
Score =  703 bits (1816), Expect = 0.0
Identities = 290/447 (64%), Positives = 343/447 (76%), Gaps = 2/447 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2345




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19487




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36048




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1171_cdd.html


    		




 
URL:

















    ReferenceID:36460




  
Author/Investigator(s):
        Hayashi M, Hirai K, Unemoto T.





  
Title:
        Sequencing and the alignment of structural genes in the nqr operon encoding the Na(+)-translocating NADH-quinone reductase from Vibrio alginolyticus.




  
PubMed:
        7729558


MedLine:
        




    
Source:
FEBS Lett. 1995 Apr 17;363(1-2):75-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=95246889&dopt=Abstract

















    ReferenceID:36467




  
Author/Investigator(s):
        Beattie P, Tan K, Bourne RM, Leach D, Rich PR, Ward FB.





  
Title:
        Cloning and sequencing of four structural genes for the Na(+)-translocating NADH-ubiquinone oxidoreductase of Vibrio alginolyticus.




  
PubMed:
        7805867


MedLine:
        




    
Source:
FEBS Lett. 1994 Dec 19;356(2-3):333-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=95104445&dopt=Abstract

















    ReferenceID:36468




  
Author/Investigator(s):
        Zhou W, Bertsova YV, Feng B, Tsatsos P, Verkhovskaya ML, Gennis RB, Bogachev AV, Barquera B.





  
Title:
        Sequencing and preliminary characterization of the Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio harveyi.




  
PubMed:
        10587447


MedLine:
        




    
Source:
Biochemistry. 1999 Dec 7;38(49):16246-52.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10587447

















    ReferenceID:36472




  
Author/Investigator(s):
        Hayashi M, Hirai K, Unemoto T.





  
Title:
        Cloning of the Na(+)-translocating NADH-quinone reductase gene from the marine bacterium Vibrio alginolyticus and the expression of the beta-subunit in Escherichia coli.




  
PubMed:
        7805866


MedLine:
        




    
Source:
FEBS Lett. 1994 Dec 19;356(2-3):330-2.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=95104444&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:1171
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2099
                           PA2999_tr
                           translation of PA2999
                           445
                           99.32
                           99.77
                           0
                           2262.0
     

     
     
70080
                           gb|AAG06387.1|AE004724_16
                           (AE004724) Na+-translocating NADH:ubiquinone oxidoreductase subunit Nrq1 [Pseudomonas aeruginosa PAO1]
                           445
                           99.32
                           99.77
                           0
                           2262.0
     

     
     
70085
                           gb|AAC43631.1|
                           (U24492) 48 kDa outer membrane protein [Actinobacillus pleuropneumoniae]
                           450
                           60.66
                           74.00
                           6
                           1357.0
     

     
     
70089
                           gb|AAP95349.1|
                           (AE017151) Na+-translocating NADH-ubiquinone oxidoreductase, subunit A [Haemophilus ducreyi 35000HP]
                           449
                           60.13
                           73.71
                           10
                           1325.0
     

     
     
70088
                           gb|AAC21836.1|
                           (U32702) NADH:ubiquinone oxidoreducatase subunit A, Na translocating [Haemophilus influenzae Rd] [Haemophilus influenzae Rd KW20]
                           452
                           59.95
                           74.77
                           12
                           1349.0
     

     






      

          
GC ORFID: 44173How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2999Glimmer Score: 

          
Start: 2742081Stop: 2740744

          
Length: 1338


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2999 translation of PA2999Homolog Bit Score 875.0

          
Other Homologs:  t_PA2999 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATCAAGATAAAACGTGGCCTGGACCTGCCCATCAGCGGCGCCCCCGAACAGCGCATCGAGGCAGCACGC 
CCGGTACGCAGCGTGGCGTTGATCGGTTTCGACTACCACGGCATGAAGCCGACCATGGCGGTGCAGGTAGGC 
GACCGGGTGAAGCTGGGGCAGGCGCTGTTCACCGACAAGAAGAATCCCTCCGTCAGCTATACCGCACCCGGC 
GCCGGTGTGGTCAGTGCCATCCATCGTGGCGAGAAGCGCGTCCTGCAATCGGTGGTCATCGATCTCGACGGC 
GACGAGCAACTGGAGTTCGCCCGTTATCCCGCGGACCAACTCGCCACGCTGAGCGCCGAGCAGGTCCGCGAC 
AACCTGTTGCAGTCGGGCCTGTGGACCGCGCTGCGGACCCGGCCGTTCAGCAAGGTGCCCGATCCCGAGTCC 
AGCCCCAGTTCGATTTTCGTCACTGCCATCGATACTCAGCCGCTGGCCGCCGATCCGCAGGTGGTTATCGCG 
GAGCAGGGCGAGGCCTTCCAGGCCGGGCTGACGGTGCTCGGGCGCCTGGCCCGGGTGTTCCTGTGCAAGGCG 
GAAGGCGTTTCGCTACCCGGCGAGGCGCTCTCCGGCGTGACCGCGCAAGCCTTCGCCGGTCCGCATCCGGCG 
GGCCTGCCCGGCACCCATATCCACTTCCTCGACCCGGTGGGTGCCGGCAAGAGCGTGTGGAACCTGAACTAC 
CAGGACGTGATCGCCATCGGCAAGCTGTTCACGACCGGGCAATTGTGGACCGAGCGGGTGATCGCCCTGGCC 
GGTCCGGTGGTGGAAAAGCCGCGTCTGGTGCGCACCCGGTTGGGCGCCAACCTCGACGAACTGGCCGCCGGC 
CAGCTGCAGCCGGGCAACAACCGGCTGATCTCCGGCTCCGTGCTGGGCGGTCGCACCGCCCATGGCGCCTAC 
GCCTACCTGGGGCGCTACCACCTGCAGTTGTCCTGTCTCAAGGAGGGCGACCAGCGCGAGTTCCTCCACTAC 
CTGCGCGCCGGGGTGGAAAAGCATTCGCTGCTCAACGTGTTCGTCTCCAGGCTGCTCGGCGGCAAGCGTTTC 
GCCTTCACCACCAGCACCAACGGCAGCCCGCGTGCGATGGTGCCGGTAGGCAATTACGAGGCGGTGATGCCG 
CTGGATATCCTGCCGACCCAACTGCTGCGCTACCTGATCGTCGGCGACACGGAAATGGCCCAGAAGCTCGGC 
GCCCTGGAACTCGACGAAGAGGACCTGGCGCTGTGCAGCTACGTCTGCGCCGGCAAGTACGAATACGGCCCG 
ATTCTGCGGGACAACCTGGCACGCATCGAGCAGGAGGGCTGA