ViewGene
Locus Name:
GeneID:
Location:

GeneID:1160
TrivialName: PA14_58050
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_58050
PAO1 Ortholog Locus:PA4472
Sequence Length:1350
Protein Length:449
Start: 5165392
Stop: 5164043
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07860.1|AE004861_1 (AE004861) PmbA protein [Pseudomonas aeruginosa PAO1]
Identities = 448/449 (99%), Positives = 448/449 (99%)
Sequence:
ATGAGTCTACATGCCGCGGCAGTCGGTCCCTCCGTGCTTCCGGACCTTCGCGAGCAGGTCGAGCAGATCATC
GCCGAGGCCCGCCGCCAGGGCGCCAGTGCCTGCGAAGTGGCGGTGTCGCTGGAGCAGGGCCTGTCCACCAGC
GTGCGCCAGGGCGAGGTGGAAACCGTCGAGTTCAACCGTGACCAAGGTTTCGGCATCACCCTCTACGCAGGC
CAGCGCAAGGGCTCGGCGAGCACCTCGGCGACCGGCGAGGCGGCCATCCGCGAGACGGTGGCGGCGGCGCTG
GCGATCGCCCGCCATACCTCCGAGGACGAATGCGCCGGTCTCGCCGATGCCGCGTTGATGGCCCGCGAGTTG
CCCGAGCTGGACCTCTATCATCCATGGAGCCTGAGTCCCGAGCAGGCCGTGGAACGAGCCCTGGCTTGCGAG
GCAGCGGCGTTCGCCGCCGACAAGCGGGTGACCAAGGCCGACGGCACCACCCTGAATACCCACCAGGGCTGC
CGGGTATACGGTAACAGCCACGGTTTCATCGGTGGTTACGCCAGCACCCGGCACAGCCTCAGCTGCGTGATG
ATCGCCGAGGGCGAAGGACAGATGCAGCGTGACTATTGGTATGACGTCAACCGCCGTGGCGAGGCCTTGGCC
AGCGCCGAGTCGATCGGTCGGCGCGCCGCCGAGCGCGCCGCCAGCCGACTGGGCGCGCGGCCGGTGCAGACC
GCCGAAGTGCCGGTGCTGTTCGCTCCGGAAATCGCTGTTGGCCTGTTCGGCCATTTCCTCGGGGCGATCTCA
GGCGGCAGCCTGTATCGCAAGTCATCCTTCCTCGAAGGCGCGCTGGGCCAGCGCCTGTTTCCCGAATGGTTG
AGCATCGACGAGCGGCCGCACCTGGTCGGCGCGCTCGGCAGCGCCAGCTTCGATAGCGACGGCCTGGCGACC
TATGCCAAGCCATTCGTCGAGAACGGCGAGCTGGTGTCCTATGTCCTGGGCACCTATTCCGGGCGCAAGCTC
GGCTTGCCGAGCACGGCCAACGCCGGCGGCGTGCATAACCTGTTCGTCAGCCATGGCGACGAGGACCAGGCC
GCTCTGATACGCCGGATGGGGCGTGGCCTGCTGGTCACCGAGTTGATGGGGCAGGGCGTCAACCTGGTGACC
GGCGATTATTCCCGCGGCGCCGCCGGCTACTGGGTCGAGAACGGCGAGATCCAGTTCCCGGTCCAGGAAGTC
ACCATCGCCGCCAACCTGCGCGACCTGTTCCGCCGTATCGTCGCGGTCGGCAAGGACATCGAACGGCGCGGC
AACCTGCATACCGGTTCTGTACTGGTCGAGTCGATGATGGTAGCCGGGCGCTGA
Translation:
MSLHAAAVGPSVLPDLREQVEQIIAEARRQGASACEVAVSLEQGLSTSVRQGEVETVEFNRDQGFGITLYAG
QRKGSASTSATGEAAIRETVAAALAIARHTSEDECAGLADAALMARELPELDLYHPWSLSPEQAVERALACE
AAAFAADKRVTKADGTTLNTHQGCRVYGNSHGFIGGYASTRHSLSCVMIAEGEGQMQRDYWYDVNRRGEALA
SAESIGRRAAERAASRLGARPVQTAEVPVLFAPEIAVGLFGHFLGAISGGSLYRKSSFLEGALGQRLFPEWL
SIDERPHLVGALGSASFDSDGLATYAKPFVENGELVSYVLGTYSGRKLGLPSTANAGGVHNLFVSHGDEDQA
ALIRRMGRGLLVTELMGQGVNLVTGDYSRGAAGYWVENGEIQFPVQEVTIAANLRDLFRRIVAVGKDIERRG
NLHTGSVLVESMMVAGR*
AnnotationID:1169GeneID:1160
AnnotatorUID: diggins
Modification Date/Time: 2005-05-03 09:09:08
Gene Name:pmbA
Confidence Code:2
GeneProduct:PmbA protein
Cell Localization:()
Synonyms:tldE
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0312,pfam01523
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57912.1| (AE016872) pmbA protein [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 448

 Score =  739 bits (1907), Expect = 0.0
 Identities = 365/442 (82%), Positives = 403/442 (91%)

>gb|AAN66567.1|AE016285_5 (AE016777) pmbA protein [Pseudomonas putida KT2440]
          Length = 452

 Score =  730 bits (1884), Expect = 0.0
 Identities = 361/442 (81%), Positives = 398/442 (90%)







 
Structural Features:
COG0312, TldD, Predicted Zn-dependent proteases and their inactivated homologs
CD-Length = 454 residues,  99.6% aligned
Score =  331 bits (851), Expect = 2e-91
Identities = 178/455 (39%), Positives = 234/455 (51%), Gaps = 26/455 (5%)

pfam01523, PmbA_TldD, Putative modulator of DNA gyrase. 
CD-Length = 297 residues,  99.0% aligned
Score =  182 bits (463), Expect = 1e-46
Identities = 98/296 (33%), Positives = 139/296 (46%), Gaps = 7/296 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2323




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19465




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31510




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1160_cdd.html


    		




 
URL:

















    ReferenceID:32862




  
Author/Investigator(s):
        Allali N, Afif H, Couturier M, Van Melderen L.





  
Title:
        The highly conserved TldD and TldE proteins of Escherichia coli are involved in microcin B17 processing and in CcdA degradation.




  
PubMed:
        12029038


MedLine:
        




    
Source:
J Bacteriol. 2002 Jun;184(12):3224-31.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12029038

















    ReferenceID:32863




  
Author/Investigator(s):
        Murayama N, Shimizu H, Takiguchi S, Baba Y, Amino H, Horiuchi T, Sekimizu K, Miki T.





  
Title:
        Evidence for involvement of Escherichia coli genes pmbA, csrA and a previously unrecognized gene tldD, in the control of DNA gyrase by letD (ccdB) of sex factor F.




  
PubMed:
        


MedLine:
        




    
Source:
J Mol Biol. 1996 Mar 1;256(3):483-502.
Erratum in:
J Mol Biol 1996 May 31;259(1):203.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8604133

















    ReferenceID:32864




  
Author/Investigator(s):
        Rodriguez-Sainz MC, Hernandez-Chico C, Moreno F.





  
Title:
        Molecular characterization of pmbA, an Escherichia coli chromosomal gene required for the production of the antibiotic peptide MccB17.




  
PubMed:
        2082149


MedLine:
        




    
Source:
Mol Microbiol. 1990 Nov;4(11):1921-32.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2082149









           
Homologs By Global Alignment 

           

       



     
Gene ID:1160
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2087
                           PA4472_tr
                           translation of PA4472
                           449
                           99.77
                           99.77
                           0
                           2267.0
     

     
     
69766
                           gb|AAG07860.1|AE004861_1
                           (AE004861) PmbA protein [Pseudomonas aeruginosa PAO1]
                           449
                           99.77
                           99.77
                           0
                           2267.0
     

     
     
69767
                           gb|AAO57912.1|
                           (AE016872) pmbA protein [Pseudomonas syringae pv. tomato str. DC3000]
                           449
                           81.73
                           90.20
                           1
                           1903.0
     

     
     
69768
                           gb|AAN66567.1|AE016285_5
                           (AE016777) pmbA protein [Pseudomonas putida KT2440]
                           453
                           80.13
                           88.30
                           5
                           1880.0
     

     
     
69773
                           gb|AAM41876.1|
                           (AE012372) PmbA protein [Xanthomonas campestris pv. campestris str. ATCC 33913]
                           458
                           51.96
                           67.90
                           12
                           1168.0
     

     






      

          
GC ORFID: 44162How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4472Glimmer Score: 

          
Start: 4429071Stop: 4427722

          
Length: 1350


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4472 translation of PA4472Homolog Bit Score 877.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5514 (ORF: BLASTX 736.0), t_PA4472 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGTCTACATGCCGCGGCAGTCGGTCCCTCCGTGCTTCCGGACCTTCGCGAGCAGGTCGAGCAGATCATC 
GCCGAGGCCCGCCGCCAGGGCGCCAGTGCCTGCGAAGTGGCGGTGTCGCTGGAGCAGGGCCTGTCCACCAGC 
GTGCGCCAGGGCGAGGTGGAAACCGTCGAGTTCAACCGTGACCAAGGTTTCGGCATCACCCTCTACGCAGGC 
CAGCGCAAGGGCTCGGCGAGCACCTCGGCGACCGGCGAGGCGGCCATCCGCGAGACGGTGGCGGCGGCGCTG 
GCGATCGCCCGCCATACCTCCGAGGACGAATGCGCCGGTCTCGCCGATGCCGCGTTGATGGCCCGCGAGTTG 
CCCGAGCTGGACCTCTATCATCCATGGAGCCTGAGTCCCGAGCAGGCCGTGGAACGAGCCCTGGCTTGCGAG 
GCAGCGGCGTTCGCCGCCGACAAGCGGGTGACCAAGGCCGACGGCACCACCCTGAATACCCACCAGGGCTGC 
CGGGTATACGGTAACAGCCACGGTTTCATCGGTGGTTACGCCAGCACCCGGCACAGCCTCAGCTGCGTGATG 
ATCGCCGAGGGCGAAGGACAGATGCAGCGTGACTATTGGTATGACGTCAACCGCCGTGGCGAGGCCTTGGCC 
AGCGCCGAGTCGATCGGTCGGCGCGCCGCCGAGCGCGCCGCCAGCCGACTGGGCGCGCGGCCGGTGCAGACC 
GCCGAAGTGCCGGTGCTGTTCGCTCCGGAAATCGCTGTTGGCCTGTTCGGCCATTTCCTCGGGGCGATCTCA 
GGCGGCAGCCTGTATCGCAAGTCATCCTTCCTCGAAGGCGCGCTGGGCCAGCGCCTGTTTCCCGAATGGTTG 
AGCATCGACGAGCGGCCGCACCTGGTCGGCGCGCTCGGCAGCGCCAGCTTCGATAGCGACGGCCTGGCGACC 
TATGCCAAGCCATTCGTCGAGAACGGCGAGCTGGTGTCCTATGTCCTGGGCACCTATTCCGGGCGCAAGCTC 
GGCTTGCCGAGCACGGCCAACGCCGGCGGCGTGCATAACCTGTTCGTCAGCCATGGCGACGAGGACCAGGCC 
GCTCTGATACGCCGGATGGGGCGTGGCCTGCTGGTCACCGAGTTGATGGGGCAGGGCGTCAACCTGGTGACC 
GGCGATTATTCCCGCGGCGCCGCCGGCTACTGGGTCGAGAACGGCGAGATCCAGTTCCCGGTCCAGGAAGTC 
ACCATCGCCGCCAACCTGCGCGACCTGTTCCGCCGTATCGTCGCGGTCGGCAAGGACATCGAACGGCGCGGC 
AACCTGCATACCGGTTCTGTACTGGTCGAGTCGATGATGGTAGCCGGGCGCTGA