ViewGene
Locus Name:
GeneID:
Location:

GeneID:115
TrivialName: PA14_23260
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_23260
PAO1 Ortholog Locus:PA3168
Sequence Length:2766
Protein Length:921
Start: 2014754
Stop: 2017519
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06556.1|AE004741_1 (AE004741) DNA gyrase subunit A [Pseudomonas aeruginosa PAO1]
Identities = 921/923 (99%), Positives = 921/923 (99%), Gaps = 2/923 (0%)
Sequence:
ATGGGCGAACTGGCCAAAGAAATTCTCCCGGTCAATATCGAAGACGAGCTGAAACAGTCCTATCTCGACTAC
GCGATGAGCGTGATCGTCGGGCGGGCCCTGCCGGATGCACGTGACGGCCTGAAGCCGGTGCACCGCCGTGTG
CTTTATGCCATGAGCGAGCTGGGCAACGACTGGAACAAGCCCTACAAGAAATCCGCCCGTGTGGTCGGCGAC
GTGATCGGTAAGTACCACCCGCACGGCGACACCGCGGTCTACGACACCATCGTGCGCATGGCGCAGCCGTTC
TCGCTGCGCTACATGCTGGTAGACGGCCAGGGCAACTTCGGTTCGGTGGACGGCGACAACGCCGCAGCCATG
CGATACACCGAAGTGCGCATGGCCAAGCTGGCCCACGAACTGCTGGCGGACCTGGAAAAGGAAACCGTCGAC
TGGGTGCCCAACTACGATGGCACCGAGCAGATCCCGGCGGTCATGCCGACCAAGATTCCCAACCTGCTGGTC
AACGGTTCCAGTGGTATCGCCGTGGGCATGGCGACCAACATCCCGCCGCACAACCTCGGCGAAGTGATCGAC
GGCTGCCTGGCGCTGATGGACAACCCCGACCTGACCGTCGATGAGCTGATGCAGTACATCCCCGGTCCGGAC
TTCCCCACCGCCGGCATCATCAACGGCCGCGCCGGGATCATCGAGGCCTACCGCACCGGTCGCGGGCGCATC
TACATCCGCGCCCGTGCCGTCGTCGAGGAGATGGAGAAGGGCGGCGGTCGCGAGCAGATCATCATCACCGAG
CTGCCGTACCAGTTGAACAAGGCGCGGTTGATCGAGAAGATCGCCGAGCTGGTGAAAGAGAAGAAGATCGAG
GGTATTTCCGAGCTGCGCGACGAGTCCGACAAGGACGGCATGCGCGTGGTCATCGAGCTGCGTCGCGGCGAG
GTGGGCGAGGTGGTCCTCAACAACCTCTATGCCCAGACCCAGCTGCAGAGCGTGTTCGGCATCAACGTGGTG
GCCCTGGTCGACGGCCAGCCGCGCACGCTGAACCTGAAGGACATGCTCGAAGTGTTCGTCCGCCACCGCCGC
GAAGTGGTGACCCGGCGTACCGTCTACGAGCTGCGCAAGGCCCGCGAGCGCGGGCACATCCTGGAAGGCCAG
GCGGTCGCCCTGTCGAACATCGATCCGGTGATCGAGCTGATCAAGAGTTCGCCGACCCCGGCCGAGGCCAAG
GAACGCCTGATCGCCACTGCCTGGGAGTCCAGCGCGGTGGAAGCGATGGTCGAGCGTGCCGGCGCCGACGCC
TGTCGCCCGGAAGACCTGGATCCGCAGTACGGCCTGCGCGACGGCAAGTACTACCTGTCGCCGGAACAGGCC
CAGGCGATCCTCGAGCTGCGCCTGCATCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTCTCCGAATACCAG
GAAATCCTCAACCTGATCGGCGAGCTGATCCGCATCCTGACCAACCCGGCGCGCCTGATGGAGGTGATCCGT
GAGGAACTGGAAGCGGTCAAGGCCGAATTCGGCGATGCTCGCCGCACCGAGATCGTGGCTTCCCAGGTCGAC
CTGACCATCGCCGACCTGATCACCGAGGAAGACCGCGTGGTGACCATTTCCCACGGCGGCTACGCCAAGTCC
CAGCCGCTGGCCGCCTACCAGGCGCAGCGTCGCGGCGGCAAAGGCAAGTCCGCCACCGGGATGAAGGACGAG
GACTACATCGAACACCTGCTGGTGGCCAACAGCCATGCGACCCTCCTGCTGTTCTCCAGCAAGGGCAAGGTC
TACTGGCTGCGTACCTTCGAGATTCCGGAGGCCTCGCGTACCGCGCGTGGCCGGCCGCTGGTGAACCTGCTG
CCGCTGGACGAGGGCGAGCGGATCACCGCGATGTTGCAGATCGACCTGGAGGCGCTGCAGCAGAACGGCGGC
GCCGATGACGACCTCGACGAAGCCGAAGGCGCGGTGCTCGAGGGCGAGGTGGTCGAGGCCGCCGAGGTCGAG
GAAGTCGAGGGCGAGACCGCCGAGCTGGTGGCCGAGCCGACCGGCGCCTACATCTTCATGGCCACCGCCTTC
GGTACCGTGAAGAAGACCCCGCTGGTGCAGTTCAGCCGTCCGCGCAGCAGCGGCCTGATCGCGCTCAAGCTG
GAAGAGGGCGACACCCTGATCGCCGCCGCGATCACCGATGGCGCCAAGGAAGTCATGCTGTTCTCCAGCGCC
GGCAAGGTGATCCGCTTCGCCGAGAGCGTGGTGCGCATCATGGGCCGCAACGCCCGCGGCGTACGCGGCATG
CGCCTGGGCAAGGGGCAGCAACTGATCTCCATGCTGATTCCGGAGTCCGGGGCGCAGATCCTCACCGCCTCC
GAGCGCGGCTTCGGCAAGCGTACCCCGCTGAGCAAGTTCCCGCGTCGCGGCCGTGGCGGCCAGGGGGTGATC
GCCATGGTCACCAACGAGCGCAACGGCGCGCTGATCGCCGCGGTACAGGTCCAGGAAGGCGAGGAGATCATG
CTGATTTCCGACCAGGGCACCCTGGTGCGGACGCGTGTCGACGAAGTCTCCCTGTCCGGCCGCAATACCCAG
GGCGTAACCCTGATCAAGCTCGCCAGCGACGAGGTACTGGTCGGTCTGGAGCGTGTCCAGGAGCCGTCGGGC
GGAGATGACGAGGACCTGCCCGAGGGCGAGGAAGCTGCCGAATCTCTGGGCGAGTCGGCCGAGTCCGAGCCC
GCGGCGGAAGCGGAAGGCAACGAAGAGTAA
Translation:
MGELAKEILPVNIEDELKQSYLDYAMSVIVGRALPDARDGLKPVHRRVLYAMSELGNDWNKPYKKSARVVGD
VIGKYHPHGDTAVYDTIVRMAQPFSLRYMLVDGQGNFGSVDGDNAAAMRYTEVRMAKLAHELLADLEKETVD
WVPNYDGTEQIPAVMPTKIPNLLVNGSSGIAVGMATNIPPHNLGEVIDGCLALMDNPDLTVDELMQYIPGPD
FPTAGIINGRAGIIEAYRTGRGRIYIRARAVVEEMEKGGGREQIIITELPYQLNKARLIEKIAELVKEKKIE
GISELRDESDKDGMRVVIELRRGEVGEVVLNNLYAQTQLQSVFGINVVALVDGQPRTLNLKDMLEVFVRHRR
EVVTRRTVYELRKARERGHILEGQAVALSNIDPVIELIKSSPTPAEAKERLIATAWESSAVEAMVERAGADA
CRPEDLDPQYGLRDGKYYLSPEQAQAILELRLHRLTGLEHEKLLSEYQEILNLIGELIRILTNPARLMEVIR
EELEAVKAEFGDARRTEIVASQVDLTIADLITEEDRVVTISHGGYAKSQPLAAYQAQRRGGKGKSATGMKDE
DYIEHLLVANSHATLLLFSSKGKVYWLRTFEIPEASRTARGRPLVNLLPLDEGERITAMLQIDLEALQQNGG
ADDDLDEAEGAVLEGEVVEAAEVEEVEGETAELVAEPTGAYIFMATAFGTVKKTPLVQFSRPRSSGLIALKL
EEGDTLIAAAITDGAKEVMLFSSAGKVIRFAESVVRIMGRNARGVRGMRLGKGQQLISMLIPESGAQILTAS
ERGFGKRTPLSKFPRRGRGGQGVIAMVTNERNGALIAAVQVQEGEEIMLISDQGTLVRTRVDEVSLSGRNTQ
GVTLIKLASDEVLVGLERVQEPSGGDDEDLPEGEEAAESLGESAESEPAAEAEGNEE*
AnnotationID:125GeneID:115
AnnotatorUID: diggins
Modification Date/Time: 2005-04-13 13:01:30
Gene Name:gyrA
Confidence Code:1
GeneProduct: DNA gyrase subunit A
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(11) DNA replication, recombination, modification and repair
Alternate Gene Product Name: DNA topoisomerase
Functional Category Confidence Code:5
COGs:COG0188,cd00187,smart00434,pfam00521
Secondary Functional Category(ies):
EC Number:5.99.1.3
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67387.1|AE016365_2 (AE016780) DNA gyrase, subunit A [Pseudomonas putida KT2440]
          Length = 923

 Score = 1539 bits (3985), Expect = 0.0
 Identities = 783/918 (85%), Positives = 851/918 (92%), Gaps = 1/918 (0%)

>gb|AAO55265.1| (AE016862) DNA gyrase, subunit A [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 928

 Score = 1447 bits (3746), Expect = 0.0
 Identities = 745/919 (81%), Positives = 817/919 (88%), Gaps = 11/919 (1%)

>gb|AAM86523.1|AE013898_6 (AE013898) DNA gyrase, subunit A, type II topoisomerase [Yersinia
           pestis KIM]
          Length = 891

 Score = 1178 bits (3048), Expect = 0.0
 Identities = 604/914 (66%), Positives = 728/914 (79%), Gaps = 49/914 (5%)








 
Structural Features:
COG0188, GyrA, Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), 
A subunit 
[DNA replication, recombination, and repair]
CD-Length = 804 residues, 100.0% aligned
Score = 1031 bits (2667), Expect = 0.0
Identities = 482/891 (54%), Positives = 630/891 (70%), Gaps = 91/891 (10%)

cd00187, TOP4c, DNA Topoisomerase, subtype IIA
CD-Length = 445 residues, 100.0% aligned
Score =  642 bits (1659), Expect = 0.0
Identities = 277/482 (57%), Positives = 335/482 (69%), Gaps = 38/482 (7%)

smart00434, TOP4c, DNA Topoisomerase IV
CD-Length = 457 residues, 100.0% aligned
Score =  612 bits (1579), Expect = 7e-176
Identities = 270/494 (54%), Positives = 336/494 (68%), Gaps = 41/494 (8%)

pfam00521, DNA_topoisoIV, DNA gyrase/topoisomerase IV, subunit A.
CD-Length = 446 residues, 100.0% aligned
Score =  661 bits (1706), Expect = 0.0
Identities = 290/484 (59%), Positives = 360/484 (74%), Gaps = 38/484 (7%)






   
Genomic Context:






   
Comment:


















    ReferenceID:233




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17375




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32374




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:115_cdd.html


    		




 
URL:

















    ReferenceID:33952




  
Author/Investigator(s):
        Kureishi A, Diver JM, Beckthold B, Schollaardt T, Bryan LE.





  
Title:
        Cloning and nucleotide sequence of Pseudomonas aeruginosa DNA gyrase gyrA gene from strain PAO1 and quinolone-resistant clinical isolates.




  
PubMed:
        7811002


MedLine:
        




    
Source:
Antimicrob Agents Chemother. 1994 Sep;38(9):1944-52.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7811002









           
Homologs By Global Alignment 

           

       



     
Gene ID:115
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
220
                           PA3168_tr
                           translation of PA3168
                           923
                           99.78
                           99.78
                           2
                           4600.0
     

     
     
15771
                           gb|AAG06556.1|AE004741_1
                           (AE004741) DNA gyrase subunit A [Pseudomonas aeruginosa PAO1]
                           923
                           99.78
                           99.78
                           2
                           4600.0
     

     
     
15772
                           gb|AAA68625.1|
                           (L29417) gyrase A subunit [Pseudomonas aeruginosa]
                           923
                           99.78
                           99.78
                           2
                           4600.0
     

     
     
15773
                           gb|AAN67387.1|AE016365_2
                           (AE016780) DNA gyrase, subunit A [Pseudomonas putida KT2440]
                           923
                           85.04
                           92.41
                           2
                           3985.0
     

     
     
15774
                           gb|AAO55265.1|
                           (AE016862) DNA gyrase, subunit A [Pseudomonas syringae pv. tomato str. DC3000]
                           936
                           80.12
                           88.14
                           23
                           3756.0
     

     






      

          
GC ORFID: 43117How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3168Glimmer Score: 

          
Start: 2939177Stop: 2936412

          
Length: 2766


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA3168 translation of PA3168Homolog Bit Score 1775.0

          
Other Homologs:  psyr_15may02_Scaffold8_revised_gene7729 (ORF: BLASTX 1449.0), t_PA3168 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGGCGAACTGGCCAAAGAAATTCTCCCGGTCAATATCGAAGACGAGCTGAAACAGTCCTATCTCGACTAC 
GCGATGAGCGTGATCGTCGGGCGGGCCCTGCCGGATGCACGTGACGGCCTGAAGCCGGTGCACCGCCGTGTG 
CTTTATGCCATGAGCGAGCTGGGCAACGACTGGAACAAGCCCTACAAGAAATCCGCCCGTGTGGTCGGCGAC 
GTGATCGGTAAGTACCACCCGCACGGCGACACCGCGGTCTACGACACCATCGTGCGCATGGCGCAGCCGTTC 
TCGCTGCGCTACATGCTGGTAGACGGCCAGGGCAACTTCGGTTCGGTGGACGGCGACAACGCCGCAGCCATG 
CGATACACCGAAGTGCGCATGGCCAAGCTGGCCCACGAACTGCTGGCGGACCTGGAAAAGGAAACCGTCGAC 
TGGGTGCCCAACTACGATGGCACCGAGCAGATCCCGGCGGTCATGCCGACCAAGATTCCCAACCTGCTGGTC 
AACGGTTCCAGTGGTATCGCCGTGGGCATGGCGACCAACATCCCGCCGCACAACCTCGGCGAAGTGATCGAC 
GGCTGCCTGGCGCTGATGGACAACCCCGACCTGACCGTCGATGAGCTGATGCAGTACATCCCCGGTCCGGAC 
TTCCCCACCGCCGGCATCATCAACGGCCGCGCCGGGATCATCGAGGCCTACCGCACCGGTCGCGGGCGCATC 
TACATCCGCGCCCGTGCCGTCGTCGAGGAGATGGAGAAGGGCGGCGGTCGCGAGCAGATCATCATCACCGAG 
CTGCCGTACCAGTTGAACAAGGCGCGGTTGATCGAGAAGATCGCCGAGCTGGTGAAAGAGAAGAAGATCGAG 
GGTATTTCCGAGCTGCGCGACGAGTCCGACAAGGACGGCATGCGCGTGGTCATCGAGCTGCGTCGCGGCGAG 
GTGGGCGAGGTGGTCCTCAACAACCTCTATGCCCAGACCCAGCTGCAGAGCGTGTTCGGCATCAACGTGGTG 
GCCCTGGTCGACGGCCAGCCGCGCACGCTGAACCTGAAGGACATGCTCGAAGTGTTCGTCCGCCACCGCCGC 
GAAGTGGTGACCCGGCGTACCGTCTACGAGCTGCGCAAGGCCCGCGAGCGCGGGCACATCCTGGAAGGCCAG 
GCGGTCGCCCTGTCGAACATCGATCCGGTGATCGAGCTGATCAAGAGTTCGCCGACCCCGGCCGAGGCCAAG 
GAACGCCTGATCGCCACTGCCTGGGAGTCCAGCGCGGTGGAAGCGATGGTCGAGCGTGCCGGCGCCGACGCC 
TGTCGCCCGGAAGACCTGGATCCGCAGTACGGCCTGCGCGACGGCAAGTACTACCTGTCGCCGGAACAGGCC 
CAGGCGATCCTCGAGCTGCGCCTGCATCGCCTGACCGGCCTGGAGCACGAGAAGCTGCTCTCCGAATACCAG 
GAAATCCTCAACCTGATCGGCGAGCTGATCCGCATCCTGACCAACCCGGCGCGCCTGATGGAGGTGATCCGT 
GAGGAACTGGAAGCGGTCAAGGCCGAATTCGGCGATGCTCGCCGCACCGAGATCGTGGCTTCCCAGGTCGAC 
CTGACCATCGCCGACCTGATCACCGAGGAAGACCGCGTGGTGACCATTTCCCACGGCGGCTACGCCAAGTCC 
CAGCCGCTGGCCGCCTACCAGGCGCAGCGTCGCGGCGGCAAAGGCAAGTCCGCCACCGGGATGAAGGACGAG 
GACTACATCGAACACCTGCTGGTGGCCAACAGCCATGCGACCCTCCTGCTGTTCTCCAGCAAGGGCAAGGTC 
TACTGGCTGCGTACCTTCGAGATTCCGGAGGCCTCGCGTACCGCGCGTGGCCGGCCGCTGGTGAACCTGCTG 
CCGCTGGACGAGGGCGAGCGGATCACCGCGATGTTGCAGATCGACCTGGAGGCGCTGCAGCAGAACGGCGGC 
GCCGATGACGACCTCGACGAAGCCGAAGGCGCGGTGCTCGAGGGCGAGGTGGTCGAGGCCGCCGAGGTCGAG 
GAAGTCGAGGGCGAGACCGCCGAGCTGGTGGCCGAGCCGACCGGCGCCTACATCTTCATGGCCACCGCCTTC 
GGTACCGTGAAGAAGACCCCGCTGGTGCAGTTCAGCCGTCCGCGCAGCAGCGGCCTGATCGCGCTCAAGCTG 
GAAGAGGGCGACACCCTGATCGCCGCCGCGATCACCGATGGCGCCAAGGAAGTCATGCTGTTCTCCAGCGCC 
GGCAAGGTGATCCGCTTCGCCGAGAGCGTGGTGCGCATCATGGGCCGCAACGCCCGCGGCGTACGCGGCATG 
CGCCTGGGCAAGGGGCAGCAACTGATCTCCATGCTGATTCCGGAGTCCGGGGCGCAGATCCTCACCGCCTCC 
GAGCGCGGCTTCGGCAAGCGTACCCCGCTGAGCAAGTTCCCGCGTCGCGGCCGTGGCGGCCAGGGGGTGATC 
GCCATGGTCACCAACGAGCGCAACGGCGCGCTGATCGCCGCGGTACAGGTCCAGGAAGGCGAGGAGATCATG 
CTGATTTCCGACCAGGGCACCCTGGTGCGGACGCGTGTCGACGAAGTCTCCCTGTCCGGCCGCAATACCCAG 
GGCGTAACCCTGATCAAGCTCGCCAGCGACGAGGTACTGGTCGGTCTGGAGCGTGTCCAGGAGCCGTCGGGC 
GGAGATGACGAGGACCTGCCCGAGGGCGAGGAAGCTGCCGAATCTCTGGGCGAGTCGGCCGAGTCCGAGCCC 
GCGGCGGAAGCGGAAGGCAACGAAGAGTAA