ViewGene
Locus Name:
GeneID:
Location:

GeneID:1144
TrivialName: PA14_00170
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_00170
PAO1 Ortholog Locus:PA0016
Sequence Length:1374
Protein Length:457
Start: 18762
Stop: 17389
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03406.1|AE004441_7 (AE004441) potassium uptake protein TrkA [Pseudomonas aeruginosa PAO1]
Identities = 456/457 (99%), Positives = 456/457 (99%)
Sequence:
ATGAAAATCATCATCCTAGGTGCCGGCCAGGTCGGCGGCACACTGGCCGAGCACCTGGCCAGCGAAGCCAAC
GACATCACCGTGGTCGACACCGACGGCGACCGCCTGCGCGACCTCGGCGACCGCCTCGACATCCGCACCGTA
CAAGGCAAGGCCTCGTTCCCCACGGTGCTGCGCCAGGCCGGCGCCGACGACGCCGACATGCTGGTGGCGGTG
ACCAATAGCGACGAGACCAACATGGTCGCCTGCCAGGTCGCCTACACCCTGTTCAACACCCCGACCAAGATC
GCCCGCGTCCGCGAGCCGGCCTACCTGACCCGTACCGGCCTGTTCGACAACGAGGCGATCCCGGTGGACGTG
CTGATCAGTCCGGAGCAGGTGGTCACCAACTACATCAAGCGCCTGATCGAACATCCCGGCGCGTTGCAGGTG
ATCGACTTCGCCGAAGGCAAGGCGCAACTGGTCGGCATCAAGGCCTACTACGGCGGCCCTCTGGTAGGGCAG
GAGCTGCGCCAGTTGCGCGAGCACATGCCGAACGTGGATACCCGCGTCGCCGCGATCTACCGCCGCAACCGG
CCGATCATTCCCCAGGGCGACACGGTGATCGAGGCCGACGACGAAGTCTTCTTCATCGCCGCCAAGGCCCAT
ATCCGCGCGGTAATGGGCGAGATGCGCAGGCTCGACGACAGCTACAAGCGGATCATCATCGCCGGCGGCGGC
AATGTCGGCGAGCGCCTGGCCGAGGCCATCGAGAGCCGCTACCAGGTGAAGATCATCGAGCGCAGCCCGCTG
CGCTGCCGGCACCTCTCGGATACCCTGGACAGCACCATCGTGCTCAACGGCAGCGCTTCCGACCGCGACCTG
CTGCTGGAGGAGAACATCGGCGAGACCGACGTGTTCCTCGCCCTGACCAACGACGACGAGGCCAACATCATG
TCCTCGCTGCTGGCCAAGCGGCTCGGCGCGAGCAAGGTGATGACCCTGATCAACAACCCGGCCTATGTCGAC
CTGGTGCAAGGCGGCGAGATCGACATCGCCATCAGCCCGCAACTGGCGACCATCGGCACCCTGCTGGCCCAC
GTTCGCCGCGGCGACATCGAGAGCGTACACTCGCTGCGCCGCGGCGCAGCCGAAGCGATCGAGGTGGTGGCC
CATGGCGACGCCAAGTCGAGCAAGGTGATCGGTCGCTCGATCAACGAGATCAAGCTGCCGCCGGGCACCACC
ATCGGCGCGCTGGTGCGCGACGAGGAAGTGCTGATCGCCCACGGCGACACCCACATCGAGAGCGGCGACCAC
GTGCTGCTGTTCCTGGTCGACAAGAAATACATCCGCGACGTCGAACGACTGTTCCAGGCCGGCCTGACGTTC
TTCTGA
Translation:
MKIIILGAGQVGGTLAEHLASEANDITVVDTDGDRLRDLGDRLDIRTVQGKASFPTVLRQAGADDADMLVAV
TNSDETNMVACQVAYTLFNTPTKIARVREPAYLTRTGLFDNEAIPVDVLISPEQVVTNYIKRLIEHPGALQV
IDFAEGKAQLVGIKAYYGGPLVGQELRQLREHMPNVDTRVAAIYRRNRPIIPQGDTVIEADDEVFFIAAKAH
IRAVMGEMRRLDDSYKRIIIAGGGNVGERLAEAIESRYQVKIIERSPLRCRHLSDTLDSTIVLNGSASDRDL
LLEENIGETDVFLALTNDDEANIMSSLLAKRLGASKVMTLINNPAYVDLVQGGEIDIAISPQLATIGTLLAH
VRRGDIESVHSLRRGAAEAIEVVAHGDAKSSKVIGRSINEIKLPPGTTIGALVRDEEVLIAHGDTHIESGDH
VLLFLVDKKYIRDVERLFQAGLTFF*
AnnotationID:1153GeneID:1144
AnnotatorUID: diggins
Modification Date/Time: 2005-03-22 11:11:09
Gene Name:trkA
Confidence Code:2
GeneProduct:potassium uptake protein TrkA
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0569,pfam02080
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN65699.1|AE016196_10 (AE016774) potassium uptake protein TrkA [Pseudomonas putida
           KT2440]
          Length = 457

 Score =  778 bits (2010), Expect = 0.0
 Identities = 391/457 (85%), Positives = 430/457 (94%)

>gb|AAO53734.1| (AE016856) Trk system potassium uptake protein TrkA [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 457

 Score =  772 bits (1993), Expect = 0.0
 Identities = 390/457 (85%), Positives = 427/457 (93%)

>emb|CAA36359.1| (X52114) TrkA protein of the constitutive K+ -transport system Trk
           [Escherichia coli]
          Length = 458

 Score =  586 bits (1510), Expect = e-166
 Identities = 296/452 (65%), Positives = 362/452 (80%), Gaps = 1/452 (0%)








 
Structural Features:
COG0569, TrkA, K+ transport systems, NAD-binding component 
[Inorganic ion transport and metabolism]
CD-Length = 225 residues, 100.0% aligned
Score =  185 bits (472), Expect = 7e-48
Identities = 90/231 (38%), Positives = 127/231 (54%), Gaps = 8/231 (3%)

pfam02080, TrkA_C, TrkA-C domain.
CD-Length = 71 residues, 100.0% aligned
Score = 54.4 bits (131), Expect = 2e-08
Identities = 21/72 (29%), Positives = 39/72 (54%), Gaps = 3/72 (4%)











   
Genomic Context:






   
Comment:


















    ReferenceID:2291




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19433




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33381




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1144_cdd.html


    		




 
URL:

















    ReferenceID:35525




  
Author/Investigator(s):
        Nakamura T, Matsuba Y, Yamamuro N, Booth IR, Unemoto T.





  
Title:
        Cloning and sequencing of a K+ transport gene (trk A) from the marine bacterium Vibrio alginolyticus.




  
PubMed:
        7948029


MedLine:
        




    
Source:
Biochim Biophys Acta. 1994 Nov 22;1219(3):701-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7948029

















    ReferenceID:35526




  
Author/Investigator(s):
        Schlosser A, Hamann A, Bossemeyer D, Schneider E, Bakker EP.





  
Title:
        NAD+ binding to the Escherichia coli K(+)-uptake protein TrkA and sequence similarity between TrkA and domains of a family of dehydrogenases suggest a role for NAD+ in bacterial transport.




  
PubMed:
        8412700


MedLine:
        




    
Source:
Mol Microbiol. 1993 Aug;9(3):533-43.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8412700









           
Homologs By Global Alignment 

           

       



     
Gene ID:1144
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2041
                           PA0016_tr
                           translation of PA0016
                           457
                           99.78
                           99.78
                           0
                           2275.0
     

     
     
68812
                           gb|AAG03406.1|AE004441_7
                           (AE004441) potassium uptake protein TrkA [Pseudomonas aeruginosa PAO1]
                           457
                           99.78
                           99.78
                           0
                           2275.0
     

     
     
68813
                           gb|AAN65699.1|AE016196_10
                           (AE016774) potassium uptake protein TrkA [Pseudomonas putida KT2440]
                           457
                           85.55
                           94.09
                           0
                           2010.0
     

     
     
68814
                           gb|AAO53734.1|
                           (AE016856) Trk system potassium uptake protein TrkA [Pseudomonas syringae pv. tomato str. DC3000]
                           457
                           85.33
                           93.43
                           0
                           1993.0
     

     
     
68815
                           gb|AAO09532.1|AE016800_137
                           (AE016800) Potassium uptake protein TrkA [Vibrio vulnificus CMCP6]
                           458
                           66.81
                           80.13
                           1
                           1558.0
     

     






      

          
GC ORFID: 44146How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0016Glimmer Score: 

          
Start: 5820099Stop: 5818726

          
Length: 1374


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0016 translation of PA0016Homolog Bit Score 880.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene4101 (ORF: BLASTX 765.0), t_PA0016 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAAATCATCATCCTAGGTGCCGGCCAGGTCGGCGGCACACTGGCCGAGCACCTGGCCAGCGAAGCCAAC 
GACATCACCGTGGTCGACACCGACGGCGACCGCCTGCGCGACCTCGGCGACCGCCTCGACATCCGCACCGTA 
CAAGGCAAGGCCTCGTTCCCCACGGTGCTGCGCCAGGCCGGCGCCGACGACGCCGACATGCTGGTGGCGGTG 
ACCAATAGCGACGAGACCAACATGGTCGCCTGCCAGGTCGCCTACACCCTGTTCAACACCCCGACCAAGATC 
GCCCGCGTCCGCGAGCCGGCCTACCTGACCCGTACCGGCCTGTTCGACAACGAGGCGATCCCGGTGGACGTG 
CTGATCAGTCCGGAGCAGGTGGTCACCAACTACATCAAGCGCCTGATCGAACATCCCGGCGCGTTGCAGGTG 
ATCGACTTCGCCGAAGGCAAGGCGCAACTGGTCGGCATCAAGGCCTACTACGGCGGCCCTCTGGTAGGGCAG 
GAGCTGCGCCAGTTGCGCGAGCACATGCCGAACGTGGATACCCGCGTCGCCGCGATCTACCGCCGCAACCGG 
CCGATCATTCCCCAGGGCGACACGGTGATCGAGGCCGACGACGAAGTCTTCTTCATCGCCGCCAAGGCCCAT 
ATCCGCGCGGTAATGGGCGAGATGCGCAGGCTCGACGACAGCTACAAGCGGATCATCATCGCCGGCGGCGGC 
AATGTCGGCGAGCGCCTGGCCGAGGCCATCGAGAGCCGCTACCAGGTGAAGATCATCGAGCGCAGCCCGCTG 
CGCTGCCGGCACCTCTCGGATACCCTGGACAGCACCATCGTGCTCAACGGCAGCGCTTCCGACCGCGACCTG 
CTGCTGGAGGAGAACATCGGCGAGACCGACGTGTTCCTCGCCCTGACCAACGACGACGAGGCCAACATCATG 
TCCTCGCTGCTGGCCAAGCGGCTCGGCGCGAGCAAGGTGATGACCCTGATCAACAACCCGGCCTATGTCGAC 
CTGGTGCAAGGCGGCGAGATCGACATCGCCATCAGCCCGCAACTGGCGACCATCGGCACCCTGCTGGCCCAC 
GTTCGCCGCGGCGACATCGAGAGCGTACACTCGCTGCGCCGCGGCGCAGCCGAAGCGATCGAGGTGGTGGCC 
CATGGCGACGCCAAGTCGAGCAAGGTGATCGGTCGCTCGATCAACGAGATCAAGCTGCCGCCGGGCACCACC 
ATCGGCGCGCTGGTGCGCGACGAGGAAGTGCTGATCGCCCACGGCGACACCCACATCGAGAGCGGCGACCAC 
GTGCTGCTGTTCCTGGTCGACAAGAAATACATCCGCGACGTCGAACGACTGTTCCAGGCCGGCCTGACGTTC 
TTCTGA