ViewGene
Locus Name:
GeneID:
Location:

GeneID:1142
TrivialName: PA14_17140
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_17140
PAO1 Ortholog Locus:PA3649
Sequence Length:1353
Protein Length:450
Start: 1469141
Stop: 1470493
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07037.1|AE004785_1 (AE004785) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 448/450 (99%), Positives = 450/450 (100%)
Sequence:
ATGAGTGCGCTTTACATGATCGTCGGTACCCTGGTGGCCCTGGGCGTGCTGGTGACGTTCCACGAATTCGGC
CACTTCTGGGTCGCGCGACGCTGCGGGGTGAAGGTCCTGCGCTTCTCCGTCGGCTTCGGCACGCCGCTGGTG
CGCTGGCACGACCGGCACGGCACCGAGTTCGTGGTGGCGGCCATTCCGCTGGGCGGCTACGTCAAGATGCTC
GACGAGCGCGAGGCGGAAGTACCGGCCCATCTGCTCGAACAGTCCTTCAATCGCAAGACGGTCCGTCAGCGG
ATCGCCATCGTCGCGGCTGGTCCCATCGCCAATTTCCTACTGGCGATTCTGTTCTTCTGGGTCGTCGCCCTG
CTCGGTAGCCAGCAGGTGCGCCCGGTCATCGGTTCCGTGGCGCCGGAGAGCCTGGCCGCCCAGGCCGGCCTG
GAGGCCGGGCAGGAACTGCTGGCGGTGGACGGCGAGCCCGTCACCGGCTGGAGCGGGGTCAACCTGCAGCTG
GTTCGCCGTCTCGGCGAGAGCGGTACGCTGGAAGTGCGGGTGCAGGAAAAGGGCTCGAATGTTGATTCCACC
CACCAGGTGCGTCTGGATGGCTGGCTGAAGGGCGAGGACAATCCCGATCCCATCGCCTCGCTGGGCATCCGG
CCCTGGCGGCCGGCCTTGCCGCCGGTGCTGGCCGAACTCGACCCCAAGGGGCCGGCGCAGGCCGCCGGGCTG
AAGCTCGGCGACCGCTTGCAGAGCATCGATGGCATCGCCGTCGACGACTGGCAGCAGGTGGTGGAAAGCGTC
CGTGCCCGTCCCGGGCAGCGCGTCCAGCTCAAGGTACTGCGCGACGGCGAAGTGCTGGATGTTGCCCTGGAG
CTGGCGGTGCGCGGCGAGGGCAAGGCCCGGAGCGGCTACATGGGGGCGGGGGTCGCCGGTACCGAATGGCCG
GCGGAAATGCTCCGCGAAGTGAGCTACGGACCGCTGGAGGCGGTGGGTCAGGCGCTTTCCCGAACCTGGACC
ATGAGCCTGCTAACGCTCGACTCCATAAAGAAAATGTTGCTCGGCGAGCTCTCGGTAAAAAACTTGAGCGGG
CCGATAACCATTGCTAAAGTGGCGGGCGCTTCAGCCCAGTCTGGCGTGGGGGATTTTCTCAATTTCCTGGCC
TATCTGAGCATTAGCTTGGGGGTTCTCAACCTGCTGCCGATCCCTGTCCTCGATGGCGGGCATCTGCTGTTC
TATCTGGTTGAGTGGGTGCGCGGGCGCCCATTATCCGAACGGGTACAGGCTTGGGGGATGCAGATCGGCATC
AGTCTGGTCGTTGGCGTGATGTTGCTCGCTCTGGTCAACGATCTGAGTCGTCTGTAG
Translation:
MSALYMIVGTLVALGVLVTFHEFGHFWVARRCGVKVLRFSVGFGTPLVRWHDRHGTEFVVAAIPLGGYVKML
DEREAEVPAHLLEQSFNRKTVRQRIAIVAAGPIANFLLAILFFWVVALLGSQQVRPVIGSVAPESLAAQAGL
EAGQELLAVDGEPVTGWSGVNLQLVRRLGESGTLEVRVQEKGSNVDSTHQVRLDGWLKGEDNPDPIASLGIR
PWRPALPPVLAELDPKGPAQAAGLKLGDRLQSIDGIAVDDWQQVVESVRARPGQRVQLKVLRDGEVLDVALE
LAVRGEGKARSGYMGAGVAGTEWPAEMLREVSYGPLEAVGQALSRTWTMSLLTLDSIKKMLLGELSVKNLSG
PITIAKVAGASAQSGVGDFLNFLAYLSISLGVLNLLPIPVLDGGHLLFYLVEWVRGRPLSERVQAWGMQIGI
SLVVGVMLLALVNDLSRL*
AnnotationID:1151GeneID:1142
AnnotatorUID: diggins
Modification Date/Time: 2005-04-21 05:05:29
Gene Name:
Confidence Code:3
GeneProduct:putative membrane-associated zinc metalloprotease
Cell Localization:()
Synonyms:yaeL
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(15) Membrane proteins
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0750
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67219.1|AE016348_11 (AE016779) membrane-associated zinc metalloprotease, putative
           [Pseudomonas putida KT2440]
          Length = 452

 Score =  718 bits (1853), Expect = 0.0
 Identities = 351/450 (78%), Positives = 402/450 (89%)

>gb|AAL19187.1| (AE008705) putative membrane-associated Zn-dependent protease
           [Salmonella typhimurium LT2]
          Length = 450

 Score =  399 bits (1024), Expect = e-110
 Identities = 211/450 (46%), Positives = 284/450 (63%), Gaps = 1/450 (0%)








 
Structural Features:
COG0750, Predicted membrane-associated Zn-dependent proteases 1 
[Cell envelope biogenesis, outer membrane]
CD-Length = 375 residues, only  68.5% aligned
Score =  154 bits (390), Expect = 4e-38
Identities = 107/257 (41%), Positives = 145/257 (56%), Gaps = 16/257 (6%)






   
Genomic Context:






   
Comment:
other possible gene name: ecfE


















    ReferenceID:2287




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19429




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31894




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1142_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:1142
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2039
                           PA3649_tr
                           translation of PA3649
                           450
                           99.55
                           99.99
                           0
                           2275.0
     

     
     
68764
                           gb|AAG07037.1|AE004785_1
                           (AE004785) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           450
                           99.55
                           99.99
                           0
                           2275.0
     

     
     
68765
                           gb|AAN67219.1|AE016348_11
                           (AE016779) membrane-associated zinc metalloprotease, putative [Pseudomonas putida KT2440]
                           452
                           77.65
                           88.93
                           2
                           1853.0
     

     
     
68766
                           gb|AAO55061.1|
                           (AE016861) membrane-associated zinc metalloprotease, putative [Pseudomonas syringae pv. tomato str. DC3000]
                           450
                           76.22
                           87.11
                           0
                           1800.0
     

     
     
68767
                           gb|AAM86678.1|AE013913_8
                           (AE013913) hypothetical protein [Yersinia pestis KIM]
                           454
                           48.45
                           64.75
                           7
                           1035.0
     

     






      

          
GC ORFID: 44144How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3649Glimmer Score: 

          
Start: 3484796Stop: 3483444

          
Length: 1353


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA3649 translation of PA3649Homolog Bit Score 880.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4626 (ORF: BLASTX 702.0), t_PA3649 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGTGCGCTTTACATGATCGTCGGTACCCTGGTGGCCCTGGGCGTGCTGGTGACGTTCCACGAATTCGGC 
CACTTCTGGGTCGCGCGACGCTGCGGGGTGAAGGTCCTGCGCTTCTCCGTCGGCTTCGGCACGCCGCTGGTG 
CGCTGGCACGACCGGCACGGCACCGAGTTCGTGGTGGCGGCCATTCCGCTGGGCGGCTACGTCAAGATGCTC 
GACGAGCGCGAGGCGGAAGTACCGGCCCATCTGCTCGAACAGTCCTTCAATCGCAAGACGGTCCGTCAGCGG 
ATCGCCATCGTCGCGGCTGGTCCCATCGCCAATTTCCTACTGGCGATTCTGTTCTTCTGGGTCGTCGCCCTG 
CTCGGTAGCCAGCAGGTGCGCCCGGTCATCGGTTCCGTGGCGCCGGAGAGCCTGGCCGCCCAGGCCGGCCTG 
GAGGCCGGGCAGGAACTGCTGGCGGTGGACGGCGAGCCCGTCACCGGCTGGAGCGGGGTCAACCTGCAGCTG 
GTTCGCCGTCTCGGCGAGAGCGGTACGCTGGAAGTGCGGGTGCAGGAAAAGGGCTCGAATGTTGATTCCACC 
CACCAGGTGCGTCTGGATGGCTGGCTGAAGGGCGAGGACAATCCCGATCCCATCGCCTCGCTGGGCATCCGG 
CCCTGGCGGCCGGCCTTGCCGCCGGTGCTGGCCGAACTCGACCCCAAGGGGCCGGCGCAGGCCGCCGGGCTG 
AAGCTCGGCGACCGCTTGCAGAGCATCGATGGCATCGCCGTCGACGACTGGCAGCAGGTGGTGGAAAGCGTC 
CGTGCCCGTCCCGGGCAGCGCGTCCAGCTCAAGGTACTGCGCGACGGCGAAGTGCTGGATGTTGCCCTGGAG 
CTGGCGGTGCGCGGCGAGGGCAAGGCCCGGAGCGGCTACATGGGGGCGGGGGTCGCCGGTACCGAATGGCCG 
GCGGAAATGCTCCGCGAAGTGAGCTACGGACCGCTGGAGGCGGTGGGTCAGGCGCTTTCCCGAACCTGGACC 
ATGAGCCTGCTAACGCTCGACTCCATAAAGAAAATGTTGCTCGGCGAGCTCTCGGTAAAAAACTTGAGCGGG 
CCGATAACCATTGCTAAAGTGGCGGGCGCTTCAGCCCAGTCTGGCGTGGGGGATTTTCTCAATTTCCTGGCC 
TATCTGAGCATTAGCTTGGGGGTTCTCAACCTGCTGCCGATCCCTGTCCTCGATGGCGGGCATCTGCTGTTC 
TATCTGGTTGAGTGGGTGCGCGGGCGCCCATTATCCGAACGGGTACAGGCTTGGGGGATGCAGATCGGCATC 
AGTCTGGTCGTTGGCGTGATGTTGCTCGCTCTGGTCAACGATCTGAGTCGTCTGTAG