ViewGene
Locus Name:
GeneID:
Location:

GeneID:1110
TrivialName: PA14_33480
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_33480
PAO1 Ortholog Locus:PA2414
Sequence Length:1320
Protein Length:439
Start: 2946044
Stop: 2947363
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05802.1|AE004668_12 (AE004668) L-sorbosone dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 435/439 (99%), Positives = 436/439 (99%)
Sequence:
ATGGCTAGCCTCCACTCGCTGCGCCCCGTCGCGCTTGCCCTCGGCCTGGTCCTGGCCGGCTGCAACCAGGGG
TCGCAGAACGATCCGCAGCAGCAGACCGGCGCCAATCCGGCGCTTCCGGAACCGAAGCACTTCCTTCTGCCG
CCGATGAAGGTACCGGAAGGCGTGGGCTGGTCGGGAGAGGCCAAGCCGACGGTGGCCCAGGGCCTGAAGATC
GAGAAGATCGCCGATGGCCTGCAACACCCTCGCCAGTTGCTGGTCTTGCCCAACGGCGATGTGCTGGTGGTG
GAGGCCAACTCGCCCGGCACCGCGCCGGTGACCACGCCGAAGCAACTCATCGCGGGGATGGTCAAGGGCCAG
TCCGGCAAGGCCGGCAAGGGCGGCAACCGCATCACCCTGCTGCGCCGAGCCGGCGCCGAAGGCCAGTGGCAG
AAGTACGTGTTCCtgGAAGGGCTGGACTCGCCGTTCGGCGTGCAGCGGGTGGGGGATTACCTGTATGTCGCC
AATACCGGGAATATCATGCGCTACCGCTACGTGCCGGGTGAAACCCGGGTCGCCGACCCGGGGATGGAGTTC
ACCGACCTGCCGGACACCATCAACCACCACTGGACCAAGGCGCTGCTCGCCAGTCCCGACGGGCGCAAGCTC
TACGTGGGCGTCGGTTCGAACAGCAACATCACCGAGAACGGCGCGGCGATCGAATACCGTCGCGCGGCGATT
CTCGAGGTGGACGTCGCCAGCCGCGGCAGCCGCATCTACGCCTCCGGCCTGCGCAACCCGACCGGCCTGCAG
TGGGAACCGGTCTCCGGCAAGCTGTGGACCATCGTCAACGAGCGCGACGAGATCGGCAACGACCTGGTCCCG
GACTACCTGACCTCGGTACGGGAGGGCGGCTTCTACGGCTGGCCCTACAGCTACTTCGGCCAGCACCTGGAT
TCGCGGGTGAAGCCGCAGCGTCCCGACCTGGTGGCGAAGGCCATCGTCCCCGACTACGCGCTGGGTTCCCAC
GTGGCCGCGCTGGGCCTGTTCTTCTACACCGGCAACGAGTTGCCCGAGCGCTATCGCAACGGCGCCTTCGTC
AGCGAGCACGGCAGTTGGAACCGCTCGCCGGTCAGCGGCTTCCAGGTGGTCCATGTGCCGTTCGCCGACGGC
AAGCCCGCCGGTGCGCCGCAGCCGGTGGTGACCGGGTTCGTCTCCGCGGACGAGAAGCAATTGTTCGGCGCG
CCGGTGGGGTTGGCGCAGGCGGCCGACGGCGCGTTGCTGATCGCCGACGACGTCGGCAACGCGATCTGGCGG
GTGAGCGCCGCGGGCACCGACTAG
Translation:
MASLHSLRPVALALGLVLAGCNQGSQNDPQQQTGANPALPEPKHFLLPPMKVPEGVGWSGEAKPTVAQGLKI
EKIADGLQHPRQLLVLPNGDVLVVEANSPGTAPVTTPKQLIAGMVKGQSGKAGKGGNRITLLRRAGAEGQWQ
KYVFLEGLDSPFGVQRVGDYLYVANTGNIMRYRYVPGETRVADPGMEFTDLPDTINHHWTKALLASPDGRKL
YVGVGSNSNITENGAAIEYRRAAILEVDVASRGSRIYASGLRNPTGLQWEPVSGKLWTIVNERDEIGNDLVP
DYLTSVREGGFYGWPYSYFGQHLDSRVKPQRPDLVAKAIVPDYALGSHVAALGLFFYTGNELPERYRNGAFV
SEHGSWNRSPVSGFQVVHVPFADGKPAGAPQPVVTGFVSADEKQLFGAPVGLAQAADGALLIADDVGNAIWR
VSAAGTD*
AnnotationID:1119GeneID:1110
AnnotatorUID: diggins
Modification Date/Time: 2005-04-29 08:08:30
Gene Name:sndH
Confidence Code:2
GeneProduct:L-sorbosone dehydrogenase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2133
Secondary Functional Category(ies):
EC Number:1.1.1.-
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAD86413.1| (BX321864) L-sorbosone dehydrogenase [Nitrosomonas europaea ATCC
           19718]
          Length = 444

 Score =  660 bits (1703), Expect = 0.0
 Identities = 313/430 (72%), Positives = 363/430 (84%), Gaps = 2/430 (0%)

>dbj|BAA05869.1| (D28511) L-sorbosone dehydrogenase [Gluconacetobacter liquefaciens]
          Length = 449

 Score =  457 bits (1177), Expect = e-128
 Identities = 228/408 (55%), Positives = 277/408 (67%), Gaps = 4/408 (0%)

>gb|AAK22762.1| (AE005753) L-sorbosone dehydrogenase [Caulobacter crescentus CB15]
          Length = 442

 Score =  457 bits (1175), Expect = e-128
 Identities = 238/431 (55%), Positives = 292/431 (67%), Gaps = 11/431 (2%)






 
Structural Features:
COG2133, COG2133, Glucose/sorbosone dehydrogenases 
[Carbohydrate transport and metabolism]
CD-Length = 399 residues,  99.5% aligned
Score =  308 bits (791), Expect = 7e-85
Identities = 157/443 (35%), Positives = 202/443 (45%), Gaps = 55/443 (12%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2223




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19365




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32960




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1110_cdd.html


    		




 
URL:

















    ReferenceID:34490




  
Author/Investigator(s):
        Shinjoh M, Tomiyama N, Asakura A, Hoshino T.





  
Title:
        Cloning and nucleotide sequencing of the membrane-bound L-sorbosone dehydrogenase gene of Acetobacter liquefaciens IFO 12258 and its expression in Gluconobacter oxydans.




  
PubMed:
        7574579


MedLine:
        




    
Source:
Appl Environ Microbiol. 1995 Feb;61(2):413-20.

Erratum in:

    * Appl Environ Microbiol 1995 May;61(5):2069.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7574579

















    ReferenceID:34491




  
Author/Investigator(s):
        Saito Y, Ishii Y, Hayashi H, Imao Y, Akashi T, Yoshikawa K, Noguchi Y, Soeda S, Yoshida M, Niwa M, Hosoda J, Shimomura K.





  
Title:
        Cloning of genes coding for L-sorbose and L-sorbosone dehydrogenases from Gluconobacter oxydans and microbial production of 2-keto-L-gulonate, a precursor of L-ascorbic acid, in a recombinant G. oxydans strain.




  
PubMed:
        9023923


MedLine:
        




    
Source:
Appl Environ Microbiol. 1997 Feb;63(2):454-60.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9023923









           
Homologs By Global Alignment 

           

       



     
Gene ID:1110
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1994
                           PA2414_tr
                           translation of PA2414
                           439
                           99.08
                           99.31
                           0
                           2292.0
     

     
     
68024
                           gb|AAG05802.1|AE004668_12
                           (AE004668) L-sorbosone dehydrogenase [Pseudomonas aeruginosa PAO1]
                           439
                           99.08
                           99.31
                           0
                           2292.0
     

     
     
68025
                           emb|CAD86413.1|
                           (BX321864) L-sorbosone dehydrogenase [Nitrosomonas europaea ATCC 19718]
                           445
                           70.56
                           82.24
                           7
                           1707.0
     

     
     
68027
                           gb|AAK22762.1|
                           (AE005753) L-sorbosone dehydrogenase [Caulobacter crescentus CB15]
                           453
                           53.20
                           65.56
                           25
                           1188.0
     

     
     
68031
                           emb|CAC46628.1|
                           (AL591789) PUTATIVE L-SORBOSONE DEHYDROGENASE (SNDH) PROTEIN [Sinorhizobium meliloti]
                           447
                           52.57
                           65.77
                           13
                           1157.0
     

     






      

          
GC ORFID: 44112How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2414Glimmer Score: 

          
Start: 2007872Stop: 2006553

          
Length: 1320


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA2414 translation of PA2414Homolog Bit Score 887.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene5921 (ORF: BLASTX 446.0), psyr_15may02_Scaffold1_revised_gene808 (ORF: BLASTX 425.0), t_PA2414 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCTAGCCTCCACTCGCTGCGCCCCGTCGCGCTTGCCCTCGGCCTGGTCCTGGCCGGCTGCAACCAGGGG 
TCGCAGAACGATCCGCAGCAGCAGACCGGCGCCAATCCGGCGCTTCCGGAACCGAAGCACTTCCTTCTGCCG 
CCGATGAAGGTACCGGAAGGCGTGGGCTGGTCGGGAGAGGCCAAGCCGACGGTGGCCCAGGGCCTGAAGATC 
GAGAAGATCGCCGATGGCCTGCAACACCCTCGCCAGTTGCTGGTCTTGCCCAACGGCGATGTGCTGGTGGTG 
GAGGCCAACTCGCCCGGCACCGCGCCGGTGACCACGCCGAAGCAACTCATCGCGGGGATGGTCAAGGGCCAG 
TCCGGCAAGGCCGGCAAGGGCGGCAACCGCATCACCCTGCTGCGCCGAGCCGGCGCCGAAGGCCAGTGGCAG 
AAGTACGTGTTCCtgGAAGGGCTGGACTCGCCGTTCGGCGTGCAGCGGGTGGGGGATTACCTGTATGTCGCC 
AATACCGGGAATATCATGCGCTACCGCTACGTGCCGGGTGAAACCCGGGTCGCCGACCCGGGGATGGAGTTC 
ACCGACCTGCCGGACACCATCAACCACCACTGGACCAAGGCGCTGCTCGCCAGTCCCGACGGGCGCAAGCTC 
TACGTGGGCGTCGGTTCGAACAGCAACATCACCGAGAACGGCGCGGCGATCGAATACCGTCGCGCGGCGATT 
CTCGAGGTGGACGTCGCCAGCCGCGGCAGCCGCATCTACGCCTCCGGCCTGCGCAACCCGACCGGCCTGCAG 
TGGGAACCGGTCTCCGGCAAGCTGTGGACCATCGTCAACGAGCGCGACGAGATCGGCAACGACCTGGTCCCG 
GACTACCTGACCTCGGTACGGGAGGGCGGCTTCTACGGCTGGCCCTACAGCTACTTCGGCCAGCACCTGGAT 
TCGCGGGTGAAGCCGCAGCGTCCCGACCTGGTGGCGAAGGCCATCGTCCCCGACTACGCGCTGGGTTCCCAC 
GTGGCCGCGCTGGGCCTGTTCTTCTACACCGGCAACGAGTTGCCCGAGCGCTATCGCAACGGCGCCTTCGTC 
AGCGAGCACGGCAGTTGGAACCGCTCGCCGGTCAGCGGCTTCCAGGTGGTCCATGTGCCGTTCGCCGACGGC 
AAGCCCGCCGGTGCGCCGCAGCCGGTGGTGACCGGGTTCGTCTCCGCGGACGAGAAGCAATTGTTCGGCGCG 
CCGGTGGGGTTGGCGCAGGCGGCCGACGGCGCGTTGCTGATCGCCGACGACGTCGGCAACGCGATCTGGCGG 
GTGAGCGCCGCGGGCACCGACTAG