ViewGene
Locus Name:
GeneID:
Location:

GeneID:1088
TrivialName: PA14_21610
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_21610
PAO1 Ortholog Locus:PA3280
Sequence Length:1317
Protein Length:438
Start: 1872900
Stop: 1874216
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06668.1|AE004750_6 (AE004750) Pyrophosphate-specific outer membrane porin OprO precursor [Pseudomonas aeruginosa PAO1]
Identities = 438/438 (100%), Positives = 438/438 (100%)
Sequence:
ATGATCCGTAAGCACTCGCTCGGCTTCGTTGCCAGCGCTCTGGCTCTGGCCGTATCTGCCCAGGCGTTCGCC
GGCACCGTGACCACCGACGGCGCCGATATCGTGATCAAGACCAAGGGCGGCCTCGAAGTCGCCACCACCGAC
AAGGAATTCAGCTTCAAGCTGGGCGGTCGCCTGCAGGCCGACTACAGCCGTTTCGACGGTTTCTATACCAAG
AATGGCAACACCGCCGACGCCGCCTACTTCCGCCGCGCCTTCATCGAACTCGGCGGCACCGCGTACAAGGAC
TGGAAATACCAGATCAACTTCGACCTGTCGCACAACACCGGCAGCTCCGACAACGGCTATTTCGACGAAGCT
TCGGTCACTTACACCGGCTTCAACCCGGTCAACCTGAAGTTCGGTCGCTTCGACCCCGACTTCGGCCTGGAA
AAGGCCACCAGCTCCAAGTGGGTGACCGCTCCCGAGCGTAACGCCGCCTACGAACTGGCCGACTGGATCAAC
ACCCACCAGGACGGCATGGGCGCCCAGGTCAACTCGACCCTCGCCGACATGGCCTACCTGTCCGCCGGCGTA
TCCGCCAAGGACGCCGACGACAGCGACGGCGACAGCGTCAAGCAGTTCAACTTCCGCGGCGTGTTCGCGCCG
ATGCACGAAGCCGGCAATGTTCTGCATGTCGGCGTGAACTATGCCTACCGCGACCTCGACGACACCGCCTTC
GACTCGCGCATCCGTCCGCGCCTGGGCATGCGCGGCATCGCCACCAGCGGCGGCAACGACGCCGGTGACAAC
GGCAACCGCGCGACCTTCGGCGGTGTCTCCAACTCGCCGGCCGGTTCCTACAAGGACGACAGCGTCTGGGGC
CTGGAAGGCGCCTGGGCGATGGGCCCGTTCTCGGCCCAGGCCGAATACCTGGCACGCAAGCTGAAGGCCGAT
GACAACGCCTACAAGGACATCAAGGCCAAGGGTTACTACGCGCAACTGGCCTACACCCTGACCGGCGAGTCC
CGCCAGTACAAGCTGGAGGGCGCCAAGTTCGACTCCGTCAAGCCGGAAAACAAGGAAATCGGCGCCTGGGAA
GTGTTCTACCGCTACGACAACATCAAGGTGGAAGACGACAACGTCGTCGCCGATACCGCCACTCGCGAAGTC
GGCGACACCAAGGCCAAGGCCCACAACCTGGGCGTGAACTGGTACGTCAACGATGCGGTGAAGATCAGCGCG
GCCTACGTCAAGGCGAAGACCGACAAGATTACCAACAACAATGGCGACGATGACGGCGACGGCTTCGTGACC
CGTTTGCAGTACGTGTTCTAA
Translation:
MIRKHSLGFVASALALAVSAQAFAGTVTTDGADIVIKTKGGLEVATTDKEFSFKLGGRLQADYSRFDGFYTK
NGNTADAAYFRRAFIELGGTAYKDWKYQINFDLSHNTGSSDNGYFDEASVTYTGFNPVNLKFGRFDPDFGLE
KATSSKWVTAPERNAAYELADWINTHQDGMGAQVNSTLADMAYLSAGVSAKDADDSDGDSVKQFNFRGVFAP
MHEAGNVLHVGVNYAYRDLDDTAFDSRIRPRLGMRGIATSGGNDAGDNGNRATFGGVSNSPAGSYKDDSVWG
LEGAWAMGPFSAQAEYLARKLKADDNAYKDIKAKGYYAQLAYTLTGESRQYKLEGAKFDSVKPENKEIGAWE
VFYRYDNIKVEDDNVVADTATREVGDTKAKAHNLGVNWYVNDAVKISAAYVKAKTDKITNNNGDDDGDGFVT
RLQYVF*
AnnotationID:1097GeneID:1088
AnnotatorUID: diggins
Modification Date/Time: 2005-04-15 06:06:21
Gene Name:oprO
Confidence Code:1
GeneProduct:pyrophosphate-specific outer membrane porin OprO precursor
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam07396,COG3746
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN65671.1|AE016194_1 (AE016774) porin P [Pseudomonas putida KT2440]
          Length = 429

 Score =  602 bits (1551), Expect = e-171
 Identities = 291/429 (67%), Positives = 350/429 (81%), Gaps = 5/429 (1%)








 
Structural Features:
pfam07396, Porin_O_P, Phosphate-selective porin O and P.
CD-Length = 361 residues, 100.0% aligned
Score =  323 bits (830), Expect = 2e-89
Identities = 149/396 (37%), Positives = 188/396 (47%), Gaps = 47/396 (11%)

COG3746, OprP, Phosphate-selective porin 
[Inorganic ion transport and metabolism]
CD-Length = 426 residues, 100.0% aligned
Score =  532 bits (1372), Expect = 3e-152
Identities = 276/439 (62%), Positives = 301/439 (68%), Gaps = 14/439 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2179




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19321




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32268




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1088_cdd.html


    		




 
URL:

















    ReferenceID:34013




  
Author/Investigator(s):
        Siehnel RJ, Egli C, Hancock RE.





  
Title:
        Polyphosphate-selective porin OprO of Pseudomonas aeruginosa: expression, purification and sequence.




  
PubMed:
        1406271


MedLine:
        




    
Source:
Mol Microbiol. 1992 Aug;6(16):2319-26.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1406271

















    ReferenceID:34014




  
Author/Investigator(s):
        Liao X, Charlebois I, Ouellet C, Morency MJ, Dewar K, Lightfoot J, Foster J, Siehnel R, Schweizer H, Lam JS, Hancock RE, Levesque RC.





  
Title:
        Physical mapping of 32 genetic markers on the Pseudomonas aeruginosa PAO1 chromosome.




  
PubMed:
        8581173


MedLine:
        




    
Source:
Microbiology. 1996 Jan;142 ( Pt 1):79-86.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8581173









           
Homologs By Global Alignment 

           

       



     
Gene ID:1088
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1960
                           PA3280_tr
                           translation of PA3280
                           438
                           99.99
                           99.99
                           0
                           2303.0
     

     
     
67075
                           gb|AAG06668.1|AE004750_6
                           (AE004750) Pyrophosphate-specific outer membrane porin OprO precursor [Pseudomonas aeruginosa PAO1]
                           438
                           99.99
                           99.99
                           0
                           2303.0
     

     
     
67076
                           gb|AAA25912.1|
                           (M86648) oprO [Pseudomonas aeruginosa]
                           438
                           99.99
                           99.99
                           0
                           2303.0
     

     
     
1961
                           PA3279_tr
                           translation of PA3279
                           443
                           72.46
                           84.87
                           8
                           1721.0
     

     
     
67077
                           gb|AAG06667.1|AE004750_5
                           (AE004750) Phosphate-specific outer membrane porin OprP precursor [Pseudomonas aeruginosa PAO1]
                           443
                           72.46
                           84.87
                           8
                           1721.0
     

     






      

          
GC ORFID: 44090How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3280Glimmer Score: 

          
Start: 3081031Stop: 3079715

          
Length: 1317


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA3280 translation of PA3280Homolog Bit Score 891.0

          
Other Homologs:  t_PA3279 (ORF: BLASTX 663.0), t_PA3280 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATCCGTAAGCACTCGCTCGGCTTCGTTGCCAGCGCTCTGGCTCTGGCCGTATCTGCCCAGGCGTTCGCC 
GGCACCGTGACCACCGACGGCGCCGATATCGTGATCAAGACCAAGGGCGGCCTCGAAGTCGCCACCACCGAC 
AAGGAATTCAGCTTCAAGCTGGGCGGTCGCCTGCAGGCCGACTACAGCCGTTTCGACGGTTTCTATACCAAG 
AATGGCAACACCGCCGACGCCGCCTACTTCCGCCGCGCCTTCATCGAACTCGGCGGCACCGCGTACAAGGAC 
TGGAAATACCAGATCAACTTCGACCTGTCGCACAACACCGGCAGCTCCGACAACGGCTATTTCGACGAAGCT 
TCGGTCACTTACACCGGCTTCAACCCGGTCAACCTGAAGTTCGGTCGCTTCGACCCCGACTTCGGCCTGGAA 
AAGGCCACCAGCTCCAAGTGGGTGACCGCTCCCGAGCGTAACGCCGCCTACGAACTGGCCGACTGGATCAAC 
ACCCACCAGGACGGCATGGGCGCCCAGGTCAACTCGACCCTCGCCGACATGGCCTACCTGTCCGCCGGCGTA 
TCCGCCAAGGACGCCGACGACAGCGACGGCGACAGCGTCAAGCAGTTCAACTTCCGCGGCGTGTTCGCGCCG 
ATGCACGAAGCCGGCAATGTTCTGCATGTCGGCGTGAACTATGCCTACCGCGACCTCGACGACACCGCCTTC 
GACTCGCGCATCCGTCCGCGCCTGGGCATGCGCGGCATCGCCACCAGCGGCGGCAACGACGCCGGTGACAAC 
GGCAACCGCGCGACCTTCGGCGGTGTCTCCAACTCGCCGGCCGGTTCCTACAAGGACGACAGCGTCTGGGGC 
CTGGAAGGCGCCTGGGCGATGGGCCCGTTCTCGGCCCAGGCCGAATACCTGGCACGCAAGCTGAAGGCCGAT 
GACAACGCCTACAAGGACATCAAGGCCAAGGGTTACTACGCGCAACTGGCCTACACCCTGACCGGCGAGTCC 
CGCCAGTACAAGCTGGAGGGCGCCAAGTTCGACTCCGTCAAGCCGGAAAACAAGGAAATCGGCGCCTGGGAA 
GTGTTCTACCGCTACGACAACATCAAGGTGGAAGACGACAACGTCGTCGCCGATACCGCCACTCGCGAAGTC 
GGCGACACCAAGGCCAAGGCCCACAACCTGGGCGTGAACTGGTACGTCAACGATGCGGTGAAGATCAGCGCG 
GCCTACGTCAAGGCGAAGACCGACAAGATTACCAACAACAATGGCGACGATGACGGCGACGGCTTCGTGACC 
CGTTTGCAGTACGTGTTCTAA