ViewGene
Locus Name:
GeneID:
Location:

GeneID:1086
TrivialName: PA14_38330
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_38330
PAO1 Ortholog Locus:PA2025
Sequence Length:1356
Protein Length:451
Start: 3417510
Stop: 3416155
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05413.1|AE004629_2 (AE004629) glutathione reductase [Pseudomonas aeruginosa PAO1]
Identities = 449/451 (99%), Positives = 450/451 (99%)
Sequence:
ATGTCTTTCGATTTCGATCTGTTCGTCATCGGCGCCGGCTCCGGGGGCGTACGCGCCGCCCGCTTCGCCGCC
GGCTTCGGTGCCCGTGTCGCGGTGGCGGAGAGTCGCTACCTGGGTGGCACCTGCGTCAACGTCGGCTGCGTG
CCGAAGAAGCTGCTGGTCTATGGCGCGCATTTCAGCGAGGACTTCGAGCAGGCCCGGGCCTATGGTTGGAGC
GCCGGCGAGGCGCAGTTCGACTGGGCGACCCTGATCGGCAACAAGAACCGCGAGATCCAGCGGCTCAACGGC
ATCTACCGCAACCTGCTGGTGAACAGCGGGGTGACTTTGCTCGAAGGTCACGCCCGTCTGCTCGATGCGCAC
AGCGTCGAGGTGGACGGCCAGCGTTTCAGCGCCAAGCACATTCTCATCGCCACCGGTGGCTGGCCGCAGGTG
CCGGACATTCCCGGCAAGGAACACGCGATCACCTCCAACGAGGCGTTCTTCCTCGAGCGCCTGCCGCGCCGT
GTGCTGGTGGTCGGCGGCGGCTACATCGCCGTCGAGTTCGCCTCGATCTTCAACGGGCTCGGCGCCGAGACC
ACCTTGCTCTACCGTCGCGACCTGTTCCTGCGCGGCTTCGACCGCAGCGTCCGCGAACACCTGCGCGACGAA
TTGGGCAAGAAGGGCCTGGACCTGCAGTTCAACAGCGACATCGCCCGTATCGACAAACAGGCCGATGGCAGC
CTGGCCGCGACCCTCAAGGACGGCCGGGTGCTGGAGGCGGACTGCGTGTTCTATGCCACCGGGCGGCGGCCG
ATGCTGGATGACCTTGGCCTGGAAAACACCGGAGTGAAGCTGACCGACAAGGGCTTCATCGCGGTCGACGAG
CATTACCAGACCAGCGAGCCGTCGATCCTCGCCCTGGGCGATGTGATCGGTCGCGTCCAGTTGACCCCCGTG
GCGCTGGCCGAGGGCATGGCGGTGGCGCGGCGGCTGTTCAAGCCGGAGGAGTACCGTCCGGTGGACTACAAG
TTGATTCCCACCGCGGTGTTCAGCCTGCCCAACATCGGCACCGTCGGCCTGACCGAGGAGGAGGCGCTGAGC
GCCGGGCACAAGGTGAAGATCTTCGAGAGCCGCTTCCGGCCGATGAAGCTGACCCTTACCGACGACCAGGAA
AAGACCCTGATGAAGCTGGTCGTGGATGCCCACGACGACAGGGTGCTGGGCTGCCACATGGTCGGCGCCGAG
GCCGGCGAGATCCTCCAGGGTATCGCGGTGGCGATGAAGGCCGGGGCGACCAAGCAGGCGTTCGACGAGACC
ATCGGCATCCACCCGACCGCGGCGGAAGAGTTCGTCACCCTGCGCACGCCGACGCGCTGA
Translation:
MSFDFDLFVIGAGSGGVRAARFAAGFGARVAVAESRYLGGTCVNVGCVPKKLLVYGAHFSEDFEQARAYGWS
AGEAQFDWATLIGNKNREIQRLNGIYRNLLVNSGVTLLEGHARLLDAHSVEVDGQRFSAKHILIATGGWPQV
PDIPGKEHAITSNEAFFLERLPRRVLVVGGGYIAVEFASIFNGLGAETTLLYRRDLFLRGFDRSVREHLRDE
LGKKGLDLQFNSDIARIDKQADGSLAATLKDGRVLEADCVFYATGRRPMLDDLGLENTGVKLTDKGFIAVDE
HYQTSEPSILALGDVIGRVQLTPVALAEGMAVARRLFKPEEYRPVDYKLIPTAVFSLPNIGTVGLTEEEALS
AGHKVKIFESRFRPMKLTLTDDQEKTLMKLVVDAHDDRVLGCHMVGAEAGEILQGIAVAMKAGATKQAFDET
IGIHPTAAEEFVTLRTPTR*
AnnotationID:1095GeneID:1086
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 17:05:36
Gene Name:gor
Confidence Code:1
GeneProduct:glutathione reductase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1249
Secondary Functional Category(ies):amino acid biosynthesis and metabolims
EC Number:1.6.4.2
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69413.1|AE016577_8 (AE016788) glutathione reductase [Pseudomonas putida KT2440]
          Length = 451

 Score =  740 bits (1910), Expect = 0.0
 Identities = 367/451 (81%), Positives = 406/451 (90%)

>emb|CAE36660.1| (BX640427) glutathione reductase [Bordetella parapertussis]
          Length = 450

 Score =  736 bits (1901), Expect = 0.0
 Identities = 362/450 (80%), Positives = 403/450 (89%)

>gb|AAN82735.1|AE016768_153 (AE016768) Glutathione reductase [Escherichia coli CFT073]
          Length = 450

 Score =  376 bits (966), Expect = e-103
 Identities = 206/452 (45%), Positives = 284/452 (62%), Gaps = 7/452 (1%)








 
Structural Features:
COG1249, Lpd, Pyruvate/2-oxoglutarate dehydrogenase complex, 
dihydrolipoamide dehydrogenase (E3) component, 
and related enzymes 
[Energy production and conversion]
CD-Length = 454 residues, 100.0% aligned
Score =  450 bits (1158), Expect = 2e-127
Identities = 186/456 (40%), Positives = 269/456 (58%), Gaps = 11/456 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2175




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19317




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32681




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1086_cdd.html


    		




 
URL:

















    ReferenceID:32682




  
Author/Investigator(s):
        Perry AC, Ni Bhriain N, Brown NL, Rouch DA.





  
Title:
        Molecular characterization of the gor gene encoding glutathione reductase from Pseudomonas aeruginosa: determinants of substrate specificity among pyridine nucleotide-disulphide oxidoreductases.




  
PubMed:
        1849605


MedLine:
        




    
Source:
Mol Microbiol. 1991 Jan;5(1):163-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1849605









           
Homologs By Global Alignment 

           

       



     
Gene ID:1086
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1958
                           PA2025_tr
                           translation of PA2025
                           451
                           99.55
                           99.77
                           0
                           2302.0
     

     
     
67005
                           gb|AAG05413.1|AE004629_2
                           (AE004629) glutathione reductase [Pseudomonas aeruginosa PAO1]
                           451
                           99.55
                           99.77
                           0
                           2302.0
     

     
     
67006
                           emb|CAA38122.1|
                           (X54201) glutathione reductase [Pseudomonas aeruginosa]
                           451
                           99.55
                           99.77
                           0
                           2302.0
     

     
     
67007
                           gb|AAN69413.1|AE016577_8
                           (AE016788) glutathione reductase [Pseudomonas putida KT2440]
                           451
                           81.37
                           90.02
                           0
                           1910.0
     

     
     
67008
                           emb|CAE32918.1|
                           (BX640444) putative glutathione reductase [Bordetella bronchiseptica] [Bordetella bronchiseptica RB50]
                           451
                           80.48
                           89.35
                           1
                           1906.0
     

     






      

          
GC ORFID: 44088How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2025Glimmer Score: 

          
Start: 1536400Stop: 1537755

          
Length: 1356


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2025 translation of PA2025Homolog Bit Score 891.0

          
Other Homologs:  psyr_15may02_Scaffold7_revised_gene7270 (ORF: BLASTX 736.0), t_PA2025 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCTTTCGATTTCGATCTGTTCGTCATCGGCGCCGGCTCCGGGGGCGTACGCGCCGCCCGCTTCGCCGCC 
GGCTTCGGTGCCCGTGTCGCGGTGGCGGAGAGTCGCTACCTGGGTGGCACCTGCGTCAACGTCGGCTGCGTG 
CCGAAGAAGCTGCTGGTCTATGGCGCGCATTTCAGCGAGGACTTCGAGCAGGCCCGGGCCTATGGTTGGAGC 
GCCGGCGAGGCGCAGTTCGACTGGGCGACCCTGATCGGCAACAAGAACCGCGAGATCCAGCGGCTCAACGGC 
ATCTACCGCAACCTGCTGGTGAACAGCGGGGTGACTTTGCTCGAAGGTCACGCCCGTCTGCTCGATGCGCAC 
AGCGTCGAGGTGGACGGCCAGCGTTTCAGCGCCAAGCACATTCTCATCGCCACCGGTGGCTGGCCGCAGGTG 
CCGGACATTCCCGGCAAGGAACACGCGATCACCTCCAACGAGGCGTTCTTCCTCGAGCGCCTGCCGCGCCGT 
GTGCTGGTGGTCGGCGGCGGCTACATCGCCGTCGAGTTCGCCTCGATCTTCAACGGGCTCGGCGCCGAGACC 
ACCTTGCTCTACCGTCGCGACCTGTTCCTGCGCGGCTTCGACCGCAGCGTCCGCGAACACCTGCGCGACGAA 
TTGGGCAAGAAGGGCCTGGACCTGCAGTTCAACAGCGACATCGCCCGTATCGACAAACAGGCCGATGGCAGC 
CTGGCCGCGACCCTCAAGGACGGCCGGGTGCTGGAGGCGGACTGCGTGTTCTATGCCACCGGGCGGCGGCCG 
ATGCTGGATGACCTTGGCCTGGAAAACACCGGAGTGAAGCTGACCGACAAGGGCTTCATCGCGGTCGACGAG 
CATTACCAGACCAGCGAGCCGTCGATCCTCGCCCTGGGCGATGTGATCGGTCGCGTCCAGTTGACCCCCGTG 
GCGCTGGCCGAGGGCATGGCGGTGGCGCGGCGGCTGTTCAAGCCGGAGGAGTACCGTCCGGTGGACTACAAG 
TTGATTCCCACCGCGGTGTTCAGCCTGCCCAACATCGGCACCGTCGGCCTGACCGAGGAGGAGGCGCTGAGC 
GCCGGGCACAAGGTGAAGATCTTCGAGAGCCGCTTCCGGCCGATGAAGCTGACCCTTACCGACGACCAGGAA 
AAGACCCTGATGAAGCTGGTCGTGGATGCCCACGACGACAGGGTGCTGGGCTGCCACATGGTCGGCGCCGAG 
GCCGGCGAGATCCTCCAGGGTATCGCGGTGGCGATGAAGGCCGGGGCGACCAAGCAGGCGTTCGACGAGACC 
ATCGGCATCCACCCGACCGCGGCGGAAGAGTTCGTCACCCTGCGCACGCCGACGCGCTGA