ViewGene
Locus Name:
GeneID:
Location:

GeneID:108
TrivialName: PA14_20010
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_20010
PAO1 Ortholog Locus:PA3408
Sequence Length:2676
Protein Length:891
Start: 1721937
Stop: 1724612
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06796.1|AE004762_2 (AE004762) Haem uptake outer membrane receptor HasR precursor [Pseudomonas aeruginosa PAO1]
Identities = 890/891 (99%), Positives = 891/891 (100%)
Sequence:
ATGAAACATCGTGGATGGAGTGCCGTGCGAGGCGGTCGCAAGGGGGCGCAACTGGCCCTGGGGCTGGGCCTG
GTCCTGCTGGGAACGGCCGCGCTACCGCTGCATGCGCAAGACGGGGCGGACTCGGCGAGCCAGCAGCAGACC
GCGCTGCGCCGGGTCCGGCTGGACATCCCGGCACAACCGCTGAACCGCGCCCTGCTGCGATTCGCCGAGCAG
GCCGGGGTCCAGGTGTTCTTCGACAGCCAGCGTTTCGCCGGTCTCGGCAGCGCAGCGGTGCACGGCGAATAT
GTGCTGGCCGACGGCCTGAGCCAGATGCTCCAGGGCAGCCCGGTGGAATACCGCTTCTCCGGCAAGGACCAA
TTGAGCCTGATCCGCGTCAGCCAGGACGACCTGGTGCAGATGTCGCCCTCGGTGATCTCCGCCGCGCGTCCG
GACGACTGGGTCTACCAGACGCCGCATTCGGTCAGCGTGATCGGCCGCGAGCAGATCGAGCGCAACCCGCCG
CGGCATGCCGCCGACATGCTCGAGGAAACCCCCGGGGTGTACTCCTCGGTGAGCCAGCAGGACCCCGGCCTG
TCGGTGAACATCCGTGGCATCCAGGACTATGGGCGGGTCAACATGTCGGTCGACGGCATGCGCCAGAACTAC
CAGCAGAGCGGCCACCAGCAGCGCAACGGGACGCTCTACGTCGATCCCGAGCTGCTCAGCGAGGTGGTCATC
GACAAGGGCGCCAGCTCGGCCATGGGTGGCGCCGGGGTGATCGGCGGGATCGCCAACTTCCGCACCCTGGAG
GCCCGCGACCTGGTCAGGCCGGGCAAGCAGGTCGGTGGCCGGGTGCGCCTCACCAGCGGCCTGGGCGGCGAC
GCCAACGGCACCCACTTCATCGGCAGCGCGGCCTTCGCCATCGGCACCGAGGTCTGGGACATGCTGGTCGCC
GCCAGCGAGCGCCACCTGGGCGACTACGACCCGGGGACCAAGGGCAGCATTGGCGAGCTGCGCACCGGCGCC
TGGTTCAATCCCGAGGCCGGGCAGCGGGTCAAGCATTCGCCGGTGGCCTACTCAGGCTATGTGATGCGCTCG
CGACTGGCCAAGCTTGGCGTCGCCCTGCCGCAGGACCAGCGCCTGCAGTTCAGCTACCTGACCACCCAGGTG
TCCTACGACGACGCCAACATGCTCAACACCGAGAACCAGGCGCTCTGGGAAAAGCTCGGCAGCAGCGACGTG
CGTGCGCAGAACTTCGCCATCGACTACGGCTACGCGCCGGACAACCCGTTGGTGGACTTCAAGGCCAAGCTC
TACTACGTCGACAACCGCAACCGCCAGCAGACCCTGCAACGCGGTATCACCCCCGGCTACTCGATCACCTAC
CAGACCGACACCTACGGCGCGCAGGCGCAGAACACCTCGACCTTCGCTCTCGACGATCTCTCCACGCTGCGC
GCCAACTATGGCCTGGAGTTCTTCTACGACAAGGTGCGGCCGGACTCCAGCCAGCCGCGGGCGAGCACCTCG
GCGGTCGGCTTCCCTGCGGCCGAAGGCATGACCCCCAAGGGCGACCGCGCCCTCGGTAGCCTGTTCGCCCGT
CTCGACTACGACTACGACGACTGGCTCAACCTCAACGCCGGGCTGCGTTACGACCGCTACCGCCTGCGCGGC
GACACCGGCTTCAACGCGCGCACCTTCATCCTTGGCACCACCCGGCAGACCGACATGCCGCTGCAATACGCC
GTCGACCGCGAGGAGGGGCGCTTCTCGCCGACCTTCGGCCTGTCGGTCAAGCCCGGCGTCGACTGGTTGCAG
TTGTTCGCCACCTACGGCAAGGGCTGGCGCCCGCCGGCGGTGACCGAGAGCCTGATCACCGGCCGCCCCCAC
GGCGGCGGCGCGGAAAACATGTACCCCAACCCGTTCCTCAGTCCGGAGCGCTCGAAGGCCTGGGAAGTCGGC
TTCAACGTGCTGAAGGAGAACCTCTGGTTCAGCGACGACCGCCTGGGCCTGAAGGTCGCCTACTTCGACACC
CGGGTCGACGACTTCATCTTCATGGGCATGGGCATGCAGCCGCCGGGCTACGGCATGGCCGGGATCGGCAAC
AGCGCCTACGTCAACAACCTCGACAGCACGCGCTTCCGTGGCGTCGAGTACCAGCTCGACTACGATGCCGGG
CTGGCCTACGGGCAGCTCTCCTACACGCACATGATCGGCAGCAACGACTTCTGTTCGAAGACCGCCTGGCTC
GGTGGCGTCACCCAGACGGTGAAGGGCAGCGGTCGCCGCCCGCCGGTGATCGACATGCGGCCGGACGAGCAG
GCCAACGCCGCCACCCATTGCAGCGCGGTGCTCGGCTCCGCCGAACACATGCCGATGGACCGCGGCTCGCTG
ACCCTGGGCATGCGCTTCTTCGACCGCAGGCTGGACGTCGGCGCCCGTGCCCGCTACAGCGAAGGCTACTCG
GTGGCCGGCGGCGCCACGGTGTCGCAGGCCGGCGTGTACCCGGCGGACTGGAAGGAATACACCGTCTACGAC
CTGTACGGCAGCTACCGGGTGAGCGACGAGCTGACCCTGCGCCTGGCCATGGAGAACGTCACCGACCGCGCC
TACCTGGTGCCGCTGGGCGACGTGCTGGCCTTCACCCTCGGCCGCGGGCGGACCCTGCAAGGCACCCTCGAA
TACCAGTTCTGA
Translation:
MKHRGWSAVRGGRKGAQLALGLGLVLLGTAALPLHAQDGADSASQQQTALRRVRLDIPAQPLNRALLRFAEQ
AGVQVFFDSQRFAGLGSAAVHGEYVLADGLSQMLQGSPVEYRFSGKDQLSLIRVSQDDLVQMSPSVISAARP
DDWVYQTPHSVSVIGREQIERNPPRHAADMLEETPGVYSSVSQQDPGLSVNIRGIQDYGRVNMSVDGMRQNY
QQSGHQQRNGTLYVDPELLSEVVIDKGASSAMGGAGVIGGIANFRTLEARDLVRPGKQVGGRVRLTSGLGGD
ANGTHFIGSAAFAIGTEVWDMLVAASERHLGDYDPGTKGSIGELRTGAWFNPEAGQRVKHSPVAYSGYVMRS
RLAKLGVALPQDQRLQFSYLTTQVSYDDANMLNTENQALWEKLGSSDVRAQNFAIDYGYAPDNPLVDFKAKL
YYVDNRNRQQTLQRGITPGYSITYQTDTYGAQAQNTSTFALDDLSTLRANYGLEFFYDKVRPDSSQPRASTS
AVGFPAAEGMTPKGDRALGSLFARLDYDYDDWLNLNAGLRYDRYRLRGDTGFNARTFILGTTRQTDMPLQYA
VDREEGRFSPTFGLSVKPGVDWLQLFATYGKGWRPPAVTESLITGRPHGGGAENMYPNPFLSPERSKAWEVG
FNVLKENLWFSDDRLGLKVAYFDTRVDDFIFMGMGMQPPGYGMAGIGNSAYVNNLDSTRFRGVEYQLDYDAG
LAYGQLSYTHMIGSNDFCSKTAWLGGVTQTVKGSGRRPPVIDMRPDEQANAATHCSAVLGSAEHMPMDRGSL
TLGMRFFDRRLDVGARARYSEGYSVAGGATVSQAGVYPADWKEYTVYDLYGSYRVSDELTLRLAMENVTDRA
YLVPLGDVLAFTLGRGRTLQGTLEYQF*
AnnotationID:118GeneID:108
AnnotatorUID: diggins
Modification Date/Time: 2005-04-18 11:11:21
Gene Name:hasR
Confidence Code:1
GeneProduct:Haem uptake outer membrane receptor HasR precursor
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:putative outer membrane receptor
Functional Category Confidence Code:5
COGs:pfam00593,COG1629,COG4771
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>dbj|BAA88490.1| (AB023289) HasA receptor protein [Pseudomonas fluorescens]
          Length = 916

 Score =  836 bits (2160), Expect = 0.0
 Identities = 450/924 (48%), Positives = 594/924 (64%), Gaps = 48/924 (5%)

>gb|AAM83904.1|AE013630_7 (AE013630) putative outer membrane receptor [Yersinia pestis KIM]
          Length = 830

 Score =  661 bits (1705), Expect = 0.0
 Identities = 362/791 (45%), Positives = 490/791 (61%), Gaps = 62/791 (7%)

>emb|CAC93388.1| (AJ414159) putative TonB dependent receptor protein [Yersinia
           pestis CO92]
          Length = 830

 Score =  661 bits (1705), Expect = 0.0
 Identities = 362/791 (45%), Positives = 490/791 (61%), Gaps = 62/791 (7%)









 
Structural Features:
pfam00593, TonB_dep_Rec, TonB dependent receptor.
CD-Length = 712 residues,  99.9% aligned
Score =  172 bits (435), Expect = 2e-43
Identities = 162/794 (20%), Positives = 239/794 (30%), Gaps = 135/794 (17%)
 
COG1629, CirA, Outer membrane receptor proteins, mostly Fe transport 
[Inorganic ion transport and metabolism]
CD-Length = 768 residues,  93.4% aligned
Score =  158 bits (400), Expect = 2e-39
Identities = 153/781 (19%), Positives = 229/781 (29%), Gaps = 86/781 (11%)

COG4771, FepA, Outer membrane receptor for ferrienterochelin and colicins 
[Inorganic ion transport and metabolism]
CD-Length = 699 residues,  96.4% aligned
Score = 97.7 bits (243), Expect = 5e-21
Identities = 166/790 (21%), Positives = 277/790 (35%), Gaps = 141/790 (17%)






   
Genomic Context:






   
Comment:


















    ReferenceID:219




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17361




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32155




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:108_cdd.html


    		




 
URL:

















    ReferenceID:34072




  
Author/Investigator(s):
        Ochsner UA, Johnson Z, Vasil ML.





  
Title:
        Genetics and regulation of two distinct haem-uptake systems, phu and has, in Pseudomonas aeruginosa.




  
PubMed:
        10658665


MedLine:
        




    
Source:
Microbiology. 2000 Jan;146 ( Pt 1):185-98.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10658665

















    ReferenceID:34073




  
Author/Investigator(s):
        Mey AR, Payne SM.





  
Title:
        Haem utilization in Vibrio cholerae involves multiple TonB-dependent haem receptors.




  
PubMed:
        11722746


MedLine:
        




    
Source:
Mol Microbiol. 2001 Nov;42(3):835-49.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11722746









           
Homologs By Global Alignment 

           

       



     
Gene ID:108
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
206
                           PA3408_tr
                           translation of PA3408
                           891
                           99.88
                           99.99
                           0
                           4657.0
     

     
     
15311
                           gb|AAG06796.1|AE004762_2
                           (AE004762) Haem uptake outer membrane receptor HasR precursor [Pseudomonas aeruginosa PAO1]
                           891
                           99.88
                           99.99
                           0
                           4657.0
     

     
     
15312
                           gb|AAD31013.1|AF127223_1
                           (AF127223) heme receptor HasR [Pseudomonas aeruginosa]
                           891
                           98.87
                           99.10
                           8
                           4606.0
     

     
     
15314
                           emb|CAE46936.1|
                           (X81195) hasR [Serratia marcescens]
                           930
                           48.38
                           63.01
                           70
                           2169.0
     

     
     
15313
                           dbj|BAA88490.1|
                           (AB023289) HasA receptor protein [Pseudomonas fluorescens]
                           939
                           48.13
                           63.36
                           71
                           2214.0
     

     






      

          
GC ORFID: 43110How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3408Glimmer Score: 

          
Start: 3231995Stop: 3229320

          
Length: 2676


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3408 translation of PA3408Homolog Bit Score 1798.0

          
Other Homologs:  t_PA3408 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAACATCGTGGATGGAGTGCCGTGCGAGGCGGTCGCAAGGGGGCGCAACTGGCCCTGGGGCTGGGCCTG 
GTCCTGCTGGGAACGGCCGCGCTACCGCTGCATGCGCAAGACGGGGCGGACTCGGCGAGCCAGCAGCAGACC 
GCGCTGCGCCGGGTCCGGCTGGACATCCCGGCACAACCGCTGAACCGCGCCCTGCTGCGATTCGCCGAGCAG 
GCCGGGGTCCAGGTGTTCTTCGACAGCCAGCGTTTCGCCGGTCTCGGCAGCGCAGCGGTGCACGGCGAATAT 
GTGCTGGCCGACGGCCTGAGCCAGATGCTCCAGGGCAGCCCGGTGGAATACCGCTTCTCCGGCAAGGACCAA 
TTGAGCCTGATCCGCGTCAGCCAGGACGACCTGGTGCAGATGTCGCCCTCGGTGATCTCCGCCGCGCGTCCG 
GACGACTGGGTCTACCAGACGCCGCATTCGGTCAGCGTGATCGGCCGCGAGCAGATCGAGCGCAACCCGCCG 
CGGCATGCCGCCGACATGCTCGAGGAAACCCCCGGGGTGTACTCCTCGGTGAGCCAGCAGGACCCCGGCCTG 
TCGGTGAACATCCGTGGCATCCAGGACTATGGGCGGGTCAACATGTCGGTCGACGGCATGCGCCAGAACTAC 
CAGCAGAGCGGCCACCAGCAGCGCAACGGGACGCTCTACGTCGATCCCGAGCTGCTCAGCGAGGTGGTCATC 
GACAAGGGCGCCAGCTCGGCCATGGGTGGCGCCGGGGTGATCGGCGGGATCGCCAACTTCCGCACCCTGGAG 
GCCCGCGACCTGGTCAGGCCGGGCAAGCAGGTCGGTGGCCGGGTGCGCCTCACCAGCGGCCTGGGCGGCGAC 
GCCAACGGCACCCACTTCATCGGCAGCGCGGCCTTCGCCATCGGCACCGAGGTCTGGGACATGCTGGTCGCC 
GCCAGCGAGCGCCACCTGGGCGACTACGACCCGGGGACCAAGGGCAGCATTGGCGAGCTGCGCACCGGCGCC 
TGGTTCAATCCCGAGGCCGGGCAGCGGGTCAAGCATTCGCCGGTGGCCTACTCAGGCTATGTGATGCGCTCG 
CGACTGGCCAAGCTTGGCGTCGCCCTGCCGCAGGACCAGCGCCTGCAGTTCAGCTACCTGACCACCCAGGTG 
TCCTACGACGACGCCAACATGCTCAACACCGAGAACCAGGCGCTCTGGGAAAAGCTCGGCAGCAGCGACGTG 
CGTGCGCAGAACTTCGCCATCGACTACGGCTACGCGCCGGACAACCCGTTGGTGGACTTCAAGGCCAAGCTC 
TACTACGTCGACAACCGCAACCGCCAGCAGACCCTGCAACGCGGTATCACCCCCGGCTACTCGATCACCTAC 
CAGACCGACACCTACGGCGCGCAGGCGCAGAACACCTCGACCTTCGCTCTCGACGATCTCTCCACGCTGCGC 
GCCAACTATGGCCTGGAGTTCTTCTACGACAAGGTGCGGCCGGACTCCAGCCAGCCGCGGGCGAGCACCTCG 
GCGGTCGGCTTCCCTGCGGCCGAAGGCATGACCCCCAAGGGCGACCGCGCCCTCGGTAGCCTGTTCGCCCGT 
CTCGACTACGACTACGACGACTGGCTCAACCTCAACGCCGGGCTGCGTTACGACCGCTACCGCCTGCGCGGC 
GACACCGGCTTCAACGCGCGCACCTTCATCCTTGGCACCACCCGGCAGACCGACATGCCGCTGCAATACGCC 
GTCGACCGCGAGGAGGGGCGCTTCTCGCCGACCTTCGGCCTGTCGGTCAAGCCCGGCGTCGACTGGTTGCAG 
TTGTTCGCCACCTACGGCAAGGGCTGGCGCCCGCCGGCGGTGACCGAGAGCCTGATCACCGGCCGCCCCCAC 
GGCGGCGGCGCGGAAAACATGTACCCCAACCCGTTCCTCAGTCCGGAGCGCTCGAAGGCCTGGGAAGTCGGC 
TTCAACGTGCTGAAGGAGAACCTCTGGTTCAGCGACGACCGCCTGGGCCTGAAGGTCGCCTACTTCGACACC 
CGGGTCGACGACTTCATCTTCATGGGCATGGGCATGCAGCCGCCGGGCTACGGCATGGCCGGGATCGGCAAC 
AGCGCCTACGTCAACAACCTCGACAGCACGCGCTTCCGTGGCGTCGAGTACCAGCTCGACTACGATGCCGGG 
CTGGCCTACGGGCAGCTCTCCTACACGCACATGATCGGCAGCAACGACTTCTGTTCGAAGACCGCCTGGCTC 
GGTGGCGTCACCCAGACGGTGAAGGGCAGCGGTCGCCGCCCGCCGGTGATCGACATGCGGCCGGACGAGCAG 
GCCAACGCCGCCACCCATTGCAGCGCGGTGCTCGGCTCCGCCGAACACATGCCGATGGACCGCGGCTCGCTG 
ACCCTGGGCATGCGCTTCTTCGACCGCAGGCTGGACGTCGGCGCCCGTGCCCGCTACAGCGAAGGCTACTCG 
GTGGCCGGCGGCGCCACGGTGTCGCAGGCCGGCGTGTACCCGGCGGACTGGAAGGAATACACCGTCTACGAC 
CTGTACGGCAGCTACCGGGTGAGCGACGAGCTGACCCTGCGCCTGGCCATGGAGAACGTCACCGACCGCGCC 
TACCTGGTGCCGCTGGGCGACGTGCTGGCCTTCACCCTCGGCCGCGGGCGGACCCTGCAAGGCACCCTCGAA 
TACCAGTTCTGA