ViewGene
Locus Name:
GeneID:
Location:

GeneID:1071
TrivialName: PA14_63540
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_63540
PAO1 Ortholog Locus:PA4808
Sequence Length:1407
Protein Length:468
Start: 5666491
Stop: 5665085
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08194.1|AE004893_12 (AE004893) L-seryl-tRNA(ser) selenium transferase [Pseudomonas aeruginosa PAO1]
Identities = 467/468 (99%), Positives = 468/468 (100%)
Sequence:
ATGAGTTCCGTTCGCTTGCCTTCGGTCGACCGCCTGCTGCGCAGCGCGGCGGCCGCTCCCCTGCACCAGCGC
TATGGCCGCGAGGCGTTGCTGGCGACCCTCCGCGACCTGCTCGACGAACTGCGCGAGCCGGCCCGACACGGT
GCCCTCGCCGAGATCGAACTGAGCGAGGCGGTGCTCGCCGGGCGCGCCGGCGAACGCCTCGCCGCGCAGCAT
GCCAGCCGCGTGCGGCGGGTGTTCAACCTGACCGGCACGGTGTTGCATACCAACCTCGGCCGCGCCCTGCTG
CCGGACGAAGCCATCGAGGCGATCACCCTGGCCGCGCGCTACCCGCTGAACCTGGAGTTCGACCTGGCCAGC
GGCAAGCGCGGCGACCGCGACGACCTGATCGCCGGGCTGATCCGCGAACTCACCGGCGCCGAGGCGGTCACC
GTGGTCAACAACAACGCGGCGGCGGTGCTGCTGGCGCTGAACAGCCTGGGGGCGCGCAAGGAAGGGATCATC
TCGCGCGGCGAGCTGATCGAGATCGGCGGCGCCTTCCGCATTCCCGACATCATGGCCCGCGCCGGCGTGCGC
TTGCATGAGGTGGGCACCACCAACCGCACCCACGCCAGGGACTACGAAGCCGCCATCGGCCCGCGCAGCGGC
CTGCTGATGCGCGTGCACACCAGCAACTACAGCGTCCAGGGCTTCACCGCCAGCGTGCCCACCGCGCAACTG
GCGGCCATCGCCCATGGCCACGGCCTGCCGCTGCTGGAAGACCTCGGCAGCGGCACCCTGGTGGACCTGACC
CGCTGGGGCTTGCCCAAGGAACCGACGGTGCAGGAAGCCCTGGCCGACGGCGCCGACATCGTTACCTTCAGC
GGCGACAAATTGCTCGGCGGGCCCCAGGCCGGGCTGATCCTCGGCAACCGCGAACTGATCGGGCGGATCAAG
AAGAACCCGCTCAAGCGCGCCCTGCGCGTCGACAAGCTGACCCTCGCCGCTCTCGAGGCGGTGCTCGGCCTG
TACCGCGATCCGGACCGCCTCGCCGAACGCCTGACCACCCTGCGCCTGCTCAGCCGCCCGGCGGCGGAGATC
CGCGCCCAGGCCGAGCGTCTGGCGCCGGCGCTGGGCGAGGCGCTCGGCGAGGGCTGGGAGGTCGCCGTGGTC
GACGCCCTGGGCATGATCGGCAGCGGCGCGCAGCCGGTGGCGCGGCTGGCCAGCGCCGCGCTGTGCCTGCGT
CCGCGGCAGCCCAGGCGCCTGCGCGGACGCGCCCTGCGCAACCTCGAGGAAGCCCTGCGCGGGCTGCCGCTG
CCGGTGATCGGTCGGCTCGACGACGACGCCCTCTGGCTCGACCTGCGCCAGCTCGACGACGAGCCGGCGTTC
CTCGCCCAGTTGCCGCGACTGCGGAGCGAGCTGTCGTGA
Translation:
MSSVRLPSVDRLLRSAAAAPLHQRYGREALLATLRDLLDELREPARHGALAEIELSEAVLAGRAGERLAAQH
ASRVRRVFNLTGTVLHTNLGRALLPDEAIEAITLAARYPLNLEFDLASGKRGDRDDLIAGLIRELTGAEAVT
VVNNNAAAVLLALNSLGARKEGIISRGELIEIGGAFRIPDIMARAGVRLHEVGTTNRTHARDYEAAIGPRSG
LLMRVHTSNYSVQGFTASVPTAQLAAIAHGHGLPLLEDLGSGTLVDLTRWGLPKEPTVQEALADGADIVTFS
GDKLLGGPQAGLILGNRELIGRIKKNPLKRALRVDKLTLAALEAVLGLYRDPDRLAERLTTLRLLSRPAAEI
RAQAERLAPALGEALGEGWEVAVVDALGMIGSGAQPVARLASAALCLRPRQPRRLRGRALRNLEEALRGLPL
PVIGRLDDDALWLDLRQLDDEPAFLAQLPRLRSELS*
AnnotationID:1080GeneID:1071
AnnotatorUID: diggins
Modification Date/Time: 2005-04-25 20:08:57
Gene Name:selA
Confidence Code:2
GeneProduct:L-seryl-tRNA(ser) selenium transferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:selenocysteine synthase
Functional Category Confidence Code:5
COGs:pfam03841,COG1921
Secondary Functional Category(ies):amino acid transport and metabolism
EC Number:2.9.1.1
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66122.1|AE016240_7 (AE016775) L-seryl-tRNA selenium transferase [Pseudomonas putida
           KT2440]
          Length = 475

 Score =  711 bits (1836), Expect = 0.0
 Identities = 366/460 (79%), Positives = 408/460 (88%)

>gb|AAK64663.1| (AE007195) SelA selenocysteine synthase [Sinorhizobium meliloti]
          Length = 466

 Score =  474 bits (1219), Expect = e-133
 Identities = 269/457 (58%), Positives = 324/457 (70%), Gaps = 11/457 (2%)

>gb|AAN82848.1|AE016768_266 (AE016768) L-seryl-tRNA(Sec) selenium transferase [Escherichia coli
           CFT073]
          Length = 463

 Score =  440 bits (1132), Expect = e-123
 Identities = 240/456 (52%), Positives = 305/456 (66%), Gaps = 5/456 (1%)






 
Structural Features:
pfam03841, SelA, L-seryl-tRNA selenium transferase.
CD-Length = 367 residues, 100.0% aligned
Score =  470 bits (1210), Expect = 2e-133
Identities = 225/373 (60%), Positives = 268/373 (71%), Gaps = 6/373 (1%)

COG1921, SelA, Selenocysteine synthase [seryl-tRNASer selenium transferase] 
[Amino acid transport and metabolism]
CD-Length = 395 residues,  95.7% aligned
Score =  381 bits (979), Expect = 1e-106
Identities = 212/391 (54%), Positives = 263/391 (67%), Gaps = 15/391 (3%)







   
Genomic Context:






   
Comment:


















    ReferenceID:2145




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19287




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35242




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1071_cdd.html


    		




 
URL:

















    ReferenceID:35995




  
Author/Investigator(s):
        Forchhammer K, Leinfelder W, Boesmiller K, Veprek B, Bock A.





  
Title:
        Selenocysteine synthase from Escherichia coli. Nucleotide sequence of the gene (selA) and purification of the protein.




  
PubMed:
        2007584


MedLine:
        




    
Source:
J Biol Chem. 1991 Apr 5;266(10):6318-23.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2007584

















    ReferenceID:35996




  
Author/Investigator(s):
        Sawers G, Heider J, Zehelein E, Bock A.





  
Title:
        Expression and operon structure of the sel genes of Escherichia coli and identification of a third selenium-containing formate dehydrogenase isoenzyme.




  
PubMed:
        1650339


MedLine:
        




    
Source:
J Bacteriol. 1991 Aug;173(16):4983-93.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1650339

















    ReferenceID:35999




  
Author/Investigator(s):
        Gursinsky T, Jager J, Andreesen JR, Sohling B.





  
Title:
        A selDABC cluster for selenocysteine incorporation in Eubacterium acidaminophilum.




  
PubMed:
        11041351


MedLine:
        




    
Source:
Arch Microbiol. 2000 Sep;174(3):200-12.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11041351









           
Homologs By Global Alignment 

           

       



     
Gene ID:1071
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1927
                           PA4808_tr
                           translation of PA4808
                           468
                           99.78
                           99.99
                           0
                           2312.0
     

     
     
66463
                           gb|AAG08194.1|AE004893_12
                           (AE004893) L-seryl-tRNA(ser) selenium transferase [Pseudomonas aeruginosa PAO1]
                           468
                           99.78
                           99.99
                           0
                           2312.0
     

     
     
66464
                           gb|AAN66122.1|AE016240_7
                           (AE016775) L-seryl-tRNA selenium transferase [Pseudomonas putida KT2440]
                           477
                           76.93
                           85.95
                           11
                           1828.0
     

     
     
66465
                           gb|AAK64663.1|
                           (AE007195) SelA selenocysteine synthase [Sinorhizobium meliloti]
                           487
                           55.85
                           67.35
                           40
                           1226.0
     

     
     
66478
                           emb|CAC93509.1|
                           (AJ414160) L-seryl-tRNA selenium transferase [Yersinia pestis CO92]
                           476
                           51.26
                           63.86
                           22
                           1086.0
     

     






      

          
GC ORFID: 44073How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4808Glimmer Score: 

          
Start: 4930171Stop: 4928765

          
Length: 1407


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4808 translation of PA4808Homolog Bit Score 895.0

          
Other Homologs:  t_PA4808 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGTTCCGTTCGCTTGCCTTCGGTCGACCGCCTGCTGCGCAGCGCGGCGGCCGCTCCCCTGCACCAGCGC 
TATGGCCGCGAGGCGTTGCTGGCGACCCTCCGCGACCTGCTCGACGAACTGCGCGAGCCGGCCCGACACGGT 
GCCCTCGCCGAGATCGAACTGAGCGAGGCGGTGCTCGCCGGGCGCGCCGGCGAACGCCTCGCCGCGCAGCAT 
GCCAGCCGCGTGCGGCGGGTGTTCAACCTGACCGGCACGGTGTTGCATACCAACCTCGGCCGCGCCCTGCTG 
CCGGACGAAGCCATCGAGGCGATCACCCTGGCCGCGCGCTACCCGCTGAACCTGGAGTTCGACCTGGCCAGC 
GGCAAGCGCGGCGACCGCGACGACCTGATCGCCGGGCTGATCCGCGAACTCACCGGCGCCGAGGCGGTCACC 
GTGGTCAACAACAACGCGGCGGCGGTGCTGCTGGCGCTGAACAGCCTGGGGGCGCGCAAGGAAGGGATCATC 
TCGCGCGGCGAGCTGATCGAGATCGGCGGCGCCTTCCGCATTCCCGACATCATGGCCCGCGCCGGCGTGCGC 
TTGCATGAGGTGGGCACCACCAACCGCACCCACGCCAGGGACTACGAAGCCGCCATCGGCCCGCGCAGCGGC 
CTGCTGATGCGCGTGCACACCAGCAACTACAGCGTCCAGGGCTTCACCGCCAGCGTGCCCACCGCGCAACTG 
GCGGCCATCGCCCATGGCCACGGCCTGCCGCTGCTGGAAGACCTCGGCAGCGGCACCCTGGTGGACCTGACC 
CGCTGGGGCTTGCCCAAGGAACCGACGGTGCAGGAAGCCCTGGCCGACGGCGCCGACATCGTTACCTTCAGC 
GGCGACAAATTGCTCGGCGGGCCCCAGGCCGGGCTGATCCTCGGCAACCGCGAACTGATCGGGCGGATCAAG 
AAGAACCCGCTCAAGCGCGCCCTGCGCGTCGACAAGCTGACCCTCGCCGCTCTCGAGGCGGTGCTCGGCCTG 
TACCGCGATCCGGACCGCCTCGCCGAACGCCTGACCACCCTGCGCCTGCTCAGCCGCCCGGCGGCGGAGATC 
CGCGCCCAGGCCGAGCGTCTGGCGCCGGCGCTGGGCGAGGCGCTCGGCGAGGGCTGGGAGGTCGCCGTGGTC 
GACGCCCTGGGCATGATCGGCAGCGGCGCGCAGCCGGTGGCGCGGCTGGCCAGCGCCGCGCTGTGCCTGCGT 
CCGCGGCAGCCCAGGCGCCTGCGCGGACGCGCCCTGCGCAACCTCGAGGAAGCCCTGCGCGGGCTGCCGCTG 
CCGGTGATCGGTCGGCTCGACGACGACGCCCTCTGGCTCGACCTGCGCCAGCTCGACGACGAGCCGGCGTTC 
CTCGCCCAGTTGCCGCGACTGCGGAGCGAGCTGTCGTGA