ViewGene
Locus Name:
GeneID:
Location:

GeneID:1070
TrivialName: PA14_59350
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_59350
PAO1 Ortholog Locus:
Sequence Length:1329
Protein Length:442
Start: 5286291
Stop: 5287619
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAP84203.1| (AY273869) PilV2 [Pseudomonas aeruginosa]
Identities = 442/442 (100%), Positives = 442/442 (100%)
Sequence:
ATGAGGAGTACGCGCAGCAGTGGATTCATCTCGATCGAACTGATGATCGCCCTCGTCGTGATCGCCATCGCG
ACCGCCGGTGGCATATCGGTCCTGATGAGCTACCTGGACGGCTTGGACGAGCAGCACGCGGCCCAGCAGCAA
CAGCAGGTGGCCAAGGCAGCGGAGAAGTACCTGAAGGACAACTTCAGCACGGTTCTGGCCAGCGCCGGCGCC
ACGGCCCCGGCGGTGATCACCGTCCCGATGCTGCGCAACACCCGTTACCTGCCCGCAGGCTTCCGCGACACC
AACATCTACGGCCAGCAATACCAGGTCCTGGCCCGCAAGCCGGCGGCCAACCAGCTCGAAACGCTGATCGTG
ACCACGGGTGGACAGGTAGCTTCCGAACTCTCGATCCGCCGGATCGCGCAGCTCATGGGAGCCACCGGGGGC
TACATCTCGAAAACCAACACCAGTATCGCCCAGGGCGCCGCCTGGCAGGTGGCCTTAAGCAATTTCGGTAGC
GCTCCCGGCGCTGGACATCTGGCGACGGCGCTGTTCTTCCAGGACGGCGCCATCGCCAACGAGTACCTCTAC
CGCAATGCCGTCCCGGGTCATCCTGAACTCAACCGGATGAATACCACGCTGGACATGGGAGGCAACAATATC
GCCGCAGCCGGGGCGATCACGGCCAGCGGCAACATCACCACCAGCGCGGACATCAGCGCGCGCAACGTGACA
GCCACTGGTACGGTGAAAGCCGGCACTGCTGACGTCGCCGGCGAGACGTACACCGGAGGCTGGTTCAGGACC
CGTGGTGACACGGGCTGGTACAACGAGAAATGGGGCGGCGGCTGGTACATGAGCGACAGCACCTGGGTGCGC
TCCTGGATGAACAAGAACGTCTACACCGGCGGCGAGATGAAAGCGGGCAAACTCACCGCCGAGGGCCGGACG
GAAGTCGGCGAGTACCTACAGCTCAAAGGCGTGGCCACCGAAGGAGCCAACTGCTCGCCGAACGGGCTGGCA
GGCATCACCAGCACCGGACTCTGGCTGTCCTGCCAAAACGGGAAATGGGGACGAACCGCCGCCTCCATGCGC
CTGAACACCACGGCCGGCGTGATCAAGGACTGGTGTACGTTGCATGGTCAGGATAGCGCCATGGTGAACTAC
GACTACGTCCGCTACGCGATCACCTGCGGCGGCCGATTCTGCGCAGTGGGCTTCAACCAGACATTTGGCACC
AACTACTCGTTCGGGCTAATCACTGAGATCGGCCCAGGCTTCAACTACCCGGAACCCTACAAGACCCCCGAC
TCGACCAACGTGACCGTTACCTGCGTGAACTAG
Translation:
MRSTRSSGFISIELMIALVVIAIATAGGISVLMSYLDGLDEQHAAQQQQQVAKAAEKYLKDNFSTVLASAGA
TAPAVITVPMLRNTRYLPAGFRDTNIYGQQYQVLARKPAANQLETLIVTTGGQVASELSIRRIAQLMGATGG
YISKTNTSIAQGAAWQVALSNFGSAPGAGHLATALFFQDGAIANEYLYRNAVPGHPELNRMNTTLDMGGNNI
AAAGAITASGNITTSADISARNVTATGTVKAGTADVAGETYTGGWFRTRGDTGWYNEKWGGGWYMSDSTWVR
SWMNKNVYTGGEMKAGKLTAEGRTEVGEYLQLKGVATEGANCSPNGLAGITSTGLWLSCQNGKWGRTAASMR
LNTTAGVIKDWCTLHGQDSAMVNYDYVRYAITCGGRFCAVGFNQTFGTNYSFGLITEIGPGFNYPEPYKTPD
STNVTVTCVN*
AnnotationID:1079GeneID:1070
AnnotatorUID: diggins
Modification Date/Time: 2006-01-30 22:10:54
Gene Name:pilV2
Confidence Code:2
GeneProduct:Type IV B pilus protein
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(16) Motility & Attachment
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam04917
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAA44099.1| (X62169) pilV [Escherichia coli]
          Length = 417

 Score =  249 bits (636), Expect = 2e-65
 Identities = 138/348 (39%), Positives = 199/348 (57%), Gaps = 10/348 (2%)

>dbj|BAB91683.1| (AP005147) typeIV prepilin [Salmonella typhimurium]
          Length = 474

 Score =  247 bits (630), Expect = 1e-64
 Identities = 142/366 (38%), Positives = 207/366 (56%), Gaps = 17/366 (4%)

>gb|AAL05425.1| (AY047316) type IV prepilin [Yersinia pseudotuberculosis]
          Length = 425

 Score =  234 bits (597), Expect = 8e-61
 Identities = 135/353 (38%), Positives = 197/353 (55%), Gaps = 20/353 (5%)







 
Structural Features:
pfam04917, Shufflon_N, Bacterial shufflon protein, 
N-terminal constant region.
CD-Length = 356 residues,  98.3% aligned
Score =  256 bits (656), Expect = 3e-69
Identities = 149/351 (42%), Positives = 206/351 (58%), Gaps = 9/351 (2%)







   
Genomic Context:






   
Comment:


















    ReferenceID:2143




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19285




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:38293




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1070_cdd.html


    		




 
URL:

















    ReferenceID:38294




  
Author/Investigator(s):
        Alm RA, Mattick JS.





  
Title:
        Identification of a gene, pilV, required for type 4 fimbrial biogenesis in Pseudomonas aeruginosa, whose product possesses a pre-pilin-like leader sequence.




  
PubMed:
        7565109


MedLine:
        




    
Source:
Mol Microbiol. 1995 May;16(3):485-96.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7565109&query_hl=8&itool=pubmed_docsum









           
Homologs By Global Alignment 

           

       



     
Gene ID:1070
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
66462
                           gb|AAP84203.1|
                           (AY273869) PilV2 [Pseudomonas aeruginosa]
                           442
                           99.99
                           99.99
                           0
                           2315.0
     

     






      

          
GC ORFID: 44072How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_RL078Glimmer Score: 

          
Start: 4549970Stop: 4551298

          
Length: 1329


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_RL078 PilV 442 Homolog: Shufflon B / type IVB pilus biosynthesis; Pathogenicity-related / E.coli / plasmid R64 (C26421) translation of RL078Homolog Bit Score 896.0

          
Other Homologs:  t_RL078 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGGAGTACGCGCAGCAGTGGATTCATCTCGATCGAACTGATGATCGCCCTCGTCGTGATCGCCATCGCG 
ACCGCCGGTGGCATATCGGTCCTGATGAGCTACCTGGACGGCTTGGACGAGCAGCACGCGGCCCAGCAGCAA 
CAGCAGGTGGCCAAGGCAGCGGAGAAGTACCTGAAGGACAACTTCAGCACGGTTCTGGCCAGCGCCGGCGCC 
ACGGCCCCGGCGGTGATCACCGTCCCGATGCTGCGCAACACCCGTTACCTGCCCGCAGGCTTCCGCGACACC 
AACATCTACGGCCAGCAATACCAGGTCCTGGCCCGCAAGCCGGCGGCCAACCAGCTCGAAACGCTGATCGTG 
ACCACGGGTGGACAGGTAGCTTCCGAACTCTCGATCCGCCGGATCGCGCAGCTCATGGGAGCCACCGGGGGC 
TACATCTCGAAAACCAACACCAGTATCGCCCAGGGCGCCGCCTGGCAGGTGGCCTTAAGCAATTTCGGTAGC 
GCTCCCGGCGCTGGACATCTGGCGACGGCGCTGTTCTTCCAGGACGGCGCCATCGCCAACGAGTACCTCTAC 
CGCAATGCCGTCCCGGGTCATCCTGAACTCAACCGGATGAATACCACGCTGGACATGGGAGGCAACAATATC 
GCCGCAGCCGGGGCGATCACGGCCAGCGGCAACATCACCACCAGCGCGGACATCAGCGCGCGCAACGTGACA 
GCCACTGGTACGGTGAAAGCCGGCACTGCTGACGTCGCCGGCGAGACGTACACCGGAGGCTGGTTCAGGACC 
CGTGGTGACACGGGCTGGTACAACGAGAAATGGGGCGGCGGCTGGTACATGAGCGACAGCACCTGGGTGCGC 
TCCTGGATGAACAAGAACGTCTACACCGGCGGCGAGATGAAAGCGGGCAAACTCACCGCCGAGGGCCGGACG 
GAAGTCGGCGAGTACCTACAGCTCAAAGGCGTGGCCACCGAAGGAGCCAACTGCTCGCCGAACGGGCTGGCA 
GGCATCACCAGCACCGGACTCTGGCTGTCCTGCCAAAACGGGAAATGGGGACGAACCGCCGCCTCCATGCGC 
CTGAACACCACGGCCGGCGTGATCAAGGACTGGTGTACGTTGCATGGTCAGGATAGCGCCATGGTGAACTAC 
GACTACGTCCGCTACGCGATCACCTGCGGCGGCCGATTCTGCGCAGTGGGCTTCAACCAGACATTTGGCACC 
AACTACTCGTTCGGGCTAATCACTGAGATCGGCCCAGGCTTCAACTACCCGGAACCCTACAAGACCCCCGAC 
TCGACCAACGTGACCGTTACCTGCGTGAACTAG