ViewGene
Locus Name:
GeneID:
Location:

GeneID:1064
TrivialName: PA14_32380
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_32380
PAO1 Ortholog Locus:PA2495
Sequence Length:1419
Protein Length:472
Start: 2814157
Stop: 2812739
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05883.1|AE004676_15 (AE004676) Multidrug efflux outer membrane protein OprN precursor [Pseudomonas aeruginosa PAO1]
Identities = 471/472 (99%), Positives = 471/472 (99%)
Sequence:
ATGATTCACGCGCAGTCGATCCGGAGCGGGCTCGCGCCCGCCCTGGGTCTGTTCAGTCTGCTGGCGCTCAGC
GCCtGCACGGTGGGTCCGGACTACCGGACCCCCGACACCGCGGCGGCGAAGATCGACGCCACGGCGAGCAAG
CCCTACGACCGCAGCCGCTTCGAAAGCCTGTGGTGGAAACAGTTCGACGATCCGACCCTGAACCAGTTGGTC
GAACAGTCGCTGAGCGGCAACCGCGACCTGCGCGTGGCCTTCGCCCGCCTGCGCGCCGCCCGCGCCCTGCGC
GACGACGTGGCCAACGATCGCTTCCCGGTGGTCACCAGCCGCGCCAGCGCCGACATCGGCAAGGGCCAGCAA
CCGGGAGTGACCGAGGACCGGGTCAACAGCGAGCGCTACGACCTTGGCCTGGATAGCGCCTGGGAGCTTGAT
CTGTTCGGGCGCATCCGCCGTCAGCTGGAGTCCAGCGACGCCCTCAGCGAAGCGGCCGAGGCCGACCTGCAG
CAACTGCAGGTCAGCCTGATCGCCGAGCTGGTGGACGCCTACGGCCAGCTGCGCGGCGCGCAACTGCGCGAG
AAGATTGCCCTGAGCAACCTGGAGAACCAGAAGGAGTCGCGCCAGCTCACCGAGCAACTGCGCGACGCCGGG
GTCGGTGCCGAACTCGACGTACTGCGCGCCGATGCGCGCCTGGCGGCCACCGCCGCCAGCGTGCCGCAACTG
CAGGCGGAAGCCGAGCGCGCCAGGCACCGTATCGCCACCCTCCTCGGCCAACGGCCGGAAGAGTTGACAGTG
GACCTTTCGCCGCGCGACCTGCCGGCGATCACCAAGGCCCTGCCGATCGGCGATCCCGGCGAGCTGCTGCGC
CGCCGACCGGACATCCGCGCCGCCGAACGGCGCCTGGCCGCCAGCACCGCCGACGTCGGCGTGGCCACCGCC
GACCTGTTCCCGCGGGTCAGCCTCAGCGGCTTCCTCGGCTTCACCGCCGGGCGGGGCTCGCAGATCGGCTCA
AGCGCCGCCCGCGCCTGGAGCGTCGGCCCGAGCATCAGTTGGGCCGCCTTCGACCTCGGCAGCGTGCGTGCC
CGCCTGCGCGGCGCCAAGGCCGACGCCGACGCCGCGCTGGCCAGCTACGAACAGCAGGTGCTGCTGGCCCTG
GAAGAATCGGCGAATGCCTTCAGCGACTATGGCAAGCGCCAGGAGCGCCTGGTCTCGCTGGTCCGCCAGTCG
GAAGCCAGCCGCGCCGCCGCGCAACAGGCGGCGATCCGCTACCGCGAAGGCACCACCGATTTCCTGGTGCTG
CTGGACGCCGAACGCGAGCAACTCTCCGCCGAAGATGCCCAGGCCCAGGCCGAGGTCGAGCTGTACCGCGGC
ATCGTGGCGATCTACCGCTCCCTCGGCGGTGGCTGGCAACCCAGCGCCTGA
Translation:
MIHAQSIRSGLAPALGLFSLLALSACTVGPDYRTPDTAAAKIDATASKPYDRSRFESLWWKQFDDPTLNQLV
EQSLSGNRDLRVAFARLRAARALRDDVANDRFPVVTSRASADIGKGQQPGVTEDRVNSERYDLGLDSAWELD
LFGRIRRQLESSDALSEAAEADLQQLQVSLIAELVDAYGQLRGAQLREKIALSNLENQKESRQLTEQLRDAG
VGAELDVLRADARLAATAASVPQLQAEAERARHRIATLLGQRPEELTVDLSPRDLPAITKALPIGDPGELLR
RRPDIRAAERRLAASTADVGVATADLFPRVSLSGFLGFTAGRGSQIGSSAARAWSVGPSISWAAFDLGSVRA
RLRGAKADADAALASYEQQVLLALEESANAFSDYGKRQERLVSLVRQSEASRAAAQQAAIRYREGTTDFLVL
LDAEREQLSAEDAQAQAEVELYRGIVAIYRSLGGGWQPSA*
AnnotationID:1073GeneID:1064
AnnotatorUID: diggins
Modification Date/Time: 2005-03-06 15:03:41
Gene Name:oprN
Confidence Code:1
GeneProduct:multidrug efflux outer membrane protein OprN precursor
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(3) Antibiotic resistance and susceptibility
Alternate Gene Product Name:outer membrane component of multidrug efflux system
Functional Category Confidence Code:5
COGs:COG1538
Secondary Functional Category(ies):transport of small molecules
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69029.1|AE016535_3 (AE016786) multidrug efflux RND outer membrane protein OprN
           [Pseudomonas putida KT2440]
          Length = 471

 Score =  697 bits (1799), Expect = 0.0
 Identities = 356/461 (77%), Positives = 407/461 (88%), Gaps = 1/461 (0%)

>gb|AAO56588.1| (AE016867) outer membrane efflux protein [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 465

 Score =  684 bits (1766), Expect = 0.0
 Identities = 349/453 (77%), Positives = 404/453 (89%)

>emb|CAE35828.1| (BX640448) outer membrane component of multidrug efflux system
           [Bordetella bronchiseptica]
          Length = 487

 Score =  357 bits (916), Expect = 9e-98
 Identities = 195/456 (42%), Positives = 278/456 (60%)






 
Structural Features:
COG1538, TolC, Outer membrane protein 
[Cell envelope biogenesis, outer membrane / Intracellular trafficking and secretion]
CD-Length = 457 residues,  98.5% aligned
Score =  235 bits (600), Expect = 9e-63
Identities = 175/454 (38%), Positives = 252/454 (55%), Gaps = 5/454 (1%)








   
Genomic Context:






   
Comment:


















    ReferenceID:2131




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19273




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33046




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1064_cdd.html


    		




 
URL:

















    ReferenceID:34501




  
Author/Investigator(s):
        Kohler T, Michea-Hamzehpour M, Henze U, Gotoh N, Curty LK, Pechere JC.





  
Title:
        Characterization of MexE-MexF-OprN, a positively regulated multidrug efflux system of Pseudomonas aeruginosa.




  
PubMed:
        9044268


MedLine:
        




    
Source:
Mol Microbiol. 1997 Jan;23(2):345-54.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9044268









           
Homologs By Global Alignment 

           

       



     
Gene ID:1064
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1921
                           PA2495_tr
                           translation of PA2495
                           472
                           99.78
                           99.78
                           0
                           2317.0
     

     
     
65956
                           gb|AAG05883.1|AE004676_15
                           (AE004676) Multidrug efflux outer membrane protein OprN precursor [Pseudomonas aeruginosa PAO1]
                           472
                           99.78
                           99.78
                           0
                           2317.0
     

     
     
65957
                           emb|CAA67865.1|
                           (X99514) hypothetical protein [Pseudomonas aeruginosa]
                           483
                           94.61
                           95.23
                           22
                           2221.0
     

     
     
65958
                           gb|AAN69029.1|AE016535_3
                           (AE016786) multidrug efflux RND outer membrane protein OprN [Pseudomonas putida KT2440]
                           478
                           74.68
                           85.98
                           13
                           1801.0
     

     
     
65959
                           gb|AAO56588.1|
                           (AE016867) outer membrane efflux protein [Pseudomonas syringae pv. tomato str. DC3000]
                           472
                           74.57
                           86.44
                           7
                           1762.0
     

     






      

          
GC ORFID: 44066How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2495Glimmer Score: 

          
Start: 2139767Stop: 2141185

          
Length: 1419


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2495 translation of PA2495Homolog Bit Score 897.0

          
Other Homologs:  psyr_15may02_Scaffold7_revised_gene7200 (ORF: BLASTX 688.0), t_PA2495 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATTCACGCGCAGTCGATCCGGAGCGGGCTCGCGCCCGCCCTGGGTCTGTTCAGTCTGCTGGCGCTCAGC 
GCCtGCACGGTGGGTCCGGACTACCGGACCCCCGACACCGCGGCGGCGAAGATCGACGCCACGGCGAGCAAG 
CCCTACGACCGCAGCCGCTTCGAAAGCCTGTGGTGGAAACAGTTCGACGATCCGACCCTGAACCAGTTGGTC 
GAACAGTCGCTGAGCGGCAACCGCGACCTGCGCGTGGCCTTCGCCCGCCTGCGCGCCGCCCGCGCCCTGCGC 
GACGACGTGGCCAACGATCGCTTCCCGGTGGTCACCAGCCGCGCCAGCGCCGACATCGGCAAGGGCCAGCAA 
CCGGGAGTGACCGAGGACCGGGTCAACAGCGAGCGCTACGACCTTGGCCTGGATAGCGCCTGGGAGCTTGAT 
CTGTTCGGGCGCATCCGCCGTCAGCTGGAGTCCAGCGACGCCCTCAGCGAAGCGGCCGAGGCCGACCTGCAG 
CAACTGCAGGTCAGCCTGATCGCCGAGCTGGTGGACGCCTACGGCCAGCTGCGCGGCGCGCAACTGCGCGAG 
AAGATTGCCCTGAGCAACCTGGAGAACCAGAAGGAGTCGCGCCAGCTCACCGAGCAACTGCGCGACGCCGGG 
GTCGGTGCCGAACTCGACGTACTGCGCGCCGATGCGCGCCTGGCGGCCACCGCCGCCAGCGTGCCGCAACTG 
CAGGCGGAAGCCGAGCGCGCCAGGCACCGTATCGCCACCCTCCTCGGCCAACGGCCGGAAGAGTTGACAGTG 
GACCTTTCGCCGCGCGACCTGCCGGCGATCACCAAGGCCCTGCCGATCGGCGATCCCGGCGAGCTGCTGCGC 
CGCCGACCGGACATCCGCGCCGCCGAACGGCGCCTGGCCGCCAGCACCGCCGACGTCGGCGTGGCCACCGCC 
GACCTGTTCCCGCGGGTCAGCCTCAGCGGCTTCCTCGGCTTCACCGCCGGGCGGGGCTCGCAGATCGGCTCA 
AGCGCCGCCCGCGCCTGGAGCGTCGGCCCGAGCATCAGTTGGGCCGCCTTCGACCTCGGCAGCGTGCGTGCC 
CGCCTGCGCGGCGCCAAGGCCGACGCCGACGCCGCGCTGGCCAGCTACGAACAGCAGGTGCTGCTGGCCCTG 
GAAGAATCGGCGAATGCCTTCAGCGACTATGGCAAGCGCCAGGAGCGCCTGGTCTCGCTGGTCCGCCAGTCG 
GAAGCCAGCCGCGCCGCCGCGCAACAGGCGGCGATCCGCTACCGCGAAGGCACCACCGATTTCCTGGTGCTG 
CTGGACGCCGAACGCGAGCAACTCTCCGCCGAAGATGCCCAGGCCCAGGCCGAGGTCGAGCTGTACCGCGGC 
ATCGTGGCGATCTACCGCTCCCTCGGCGGTGGCTGGCAACCCAGCGCCTGA