ViewGene
Locus Name:
GeneID:
Location:

GeneID:105
TrivialName: PA14_66290
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66290
PAO1 Ortholog Locus:PA5015
Sequence Length:2649
Protein Length:882
Start: 5907553
Stop: 5910201
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08400.1|AE004914_1 (AE004914) pyruvate dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 882/882 (100%), Positives = 882/882 (100%)
Sequence:
ATGCAAGACCTCGATCCCGTCGAAACCCAGGAATGGCTGGACGCCCTGGAGTCCGTACTCGACCGCGAAGGT
GAAGACCGCGCTCATTACCTGATGACCCGCATGGGCGAGCTGGCCAGCCGTAGCGGCACCCAACTGCCCTAC
GCGATCACCACGCCCTACCGCAACACCATTCCGGTAACCCACGAAGCACGCATGCCCGGCGACCTGTTCATG
GAGCGCCGGATCCGCTCCCTGGTGCGCTGGAACGCCCTGGCGATGGTGATGCGGGCGAACAAGCACGACCCG
GACCTGGGCGGCCACATCTCCACCTTCGCCTCCTCGGCGACCCTCTACGACATCGGCTTCAACTATTTCTTC
CAGGCTCCCACCGACGAACATGGCGGCGACCTGGTGTTCTTCCAGGGCCACGCGTCCCCCGGCGTCTACGCC
CGCGCCTTCCTCGAAGGCCGCATCAGCGAGGAACAACTGGAGAACTTCCGCCAGGAAGTGGACGGCAACGGC
CTGTCCTCCTATCCGCACCCCTGGCTGATGCCGGACTTCTGGCAGTTCCCCACCGTATCCATGGGCCTTGGC
CCGATCCAGGCGATCTACCAGGCACGCTTCATGAAGTACCTGGAAAGCCGCGGCTTCATCCCCGCCGGCAAG
CAGAAGGTCTGGTGCTTCATGGGCGACGGCGAGTGCGACGAGCCGGAATCCCTCGGCGCGATCTCCCTGGCC
GGCCGCGAGAAACTCGACAACCTGATCTTCGTCATCAACTGCAACCTGCAGCGCCTCGACGGCCCGGTCCGC
GGCAACGCCAAGATCATCCAGGAACTGGAAGGCGTGTTCCGCGGCGCCGAGTGGAACGTCAACAAGGTCATC
TGGGGTCGCTTCTGGGACCCGCTGTTCGCCAAGGACACCGCCGGCCTGCTGCAGCAGCGCATGGACGAGGTC
ATCGACGGCGAATACCAGAACTACAAGGCGAAAGACGGCGCCTACGTCCGCGAGCACTTCTTCGGCGCGCGT
CCGGAACTGCTGGAAATGGTCAAGGACCTTTCCGACGAGGAAATCTGGAAGCTCAACCGCGGCGGCCACGAC
CCCTACAAGGTCTATGCGGCCTACCACCAGGCGGTCAACCACAAGGGCCAGCCGACCGTCATCCTGGCCAAG
ACCATCAAGGGCTACGGCACCGGCAGCGGCGAAGCGAAGAACATCGCGCACAACGTGAAGAAGGTCGACGTC
GACAGCCTGCGCGCGTTCCGCGACAAGTTCGACATCCCGGTCAAGGACGCCGACCTGGAGAAGCTGCCGTTC
TACAAGCCTGAAGAAGGCAGCGCCGAGGCCAAGTACCTGGCCGAGCGCCGCGCCGCGCTGGGCGGCTTCATG
CCGGTGCGCCGGCAGAAGAGCATGAGCGTGCCGGTTCCGCCGCTGGAAACCCTGAAGGCCATGCTCGACGGC
TCCGGCGATCGCGAGATCTCCACCACCATGGCCTTCGTGCGGATCATCTCGCAGCTGGTCAAGGACAAGGAA
CTCGGCCCGCGCATCGTCCCGATCGTCCCGGACGAGGCCCGTACCTTCGGCATGGAAGGCATGTTCCGCCAG
CTCGGCATCTACTCCTCGGTCGGCCAGCTCTACGAGCCGGTGGACAAGGACCAGGTGATGTTCTACCGCGAG
GACAAGAAGGGCCAGAtccTCGAGGAAGGCATCAACGAGGCCGGCGCCATGTCCAGCTGGATTGCCGCCGGT
ACCTCCTACAGCACCCACAACCAGCCGATGCTGCCGTTCTACATCTTCTATTCGATGTTCGGCTTCCAGCGC
ATCGGCGACCTGGCCTGGGCCGCCGGCGACAGCCGCGCGCACGGCTTCCTGATCGGCGGCACCGCCGGTCGC
ACCACGCTGAACGGCGAAGGCCTGCAGCACGAGGACGGCCACAGCCACCTGCTGGCCTCGACCATCCCGAAC
TGCCGCACCTACGATCCGACCTACGCCTACGAACTGGCGGTGATCATCCGCGAAGGCTCGCGGCAGATGATC
GAAGAGCAGCAGGACATCTTCTACTACATCACCGTGATGAACGAGAACTACGTCCAGCCGGCCATGCCGAAG
GGCGCAGAGGAAGGCATCATCAAGGGCATGTACCTCCTCGAGGAGGACAAGAAGGAAGCCGCTCACCACGTG
CAACTGCTGGGCTCCGGCACCATCCTGCGCGAAGTCGAGGAAGCGGCCAAGCTGCTGCGCAACGACTTCGGT
ATCGGCGCCGACGTCTGGAGCGTGCCGAGCTTCAACGAGCTGCGTCGCGACGGCCTTGCCGTCGAGCGCTGG
AACCGCCTGCATCCGGGCCAGAAGCCGAAGCAGAGCTACGTCGAGGAGTGCCTGGGCGGCCGTCGCGGCCCG
GTGATCGCCTCCACCGACTACATGAAGCTCTACGCCGAGCAGATCCGCCAGTGGGTTCCCTCCAAGGAGTAC
AAGGTGCTCGGCACCGACGGCTTCGGCCGCAGCGACAGCCGCAAGAAGCTGCGCAACTTCTTCGAAGTGGAT
CGTCACTGGGTGGTGCTGGCCGCGCTCGAAGCGCTGGCCGATCGCGGCGATATCGAGCCGAAAGTGGTGGCC
GAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAA
Translation:
MQDLDPVETQEWLDALESVLDREGEDRAHYLMTRMGELASRSGTQLPYAITTPYRNTIPVTHEARMPGDLFM
ERRIRSLVRWNALAMVMRANKHDPDLGGHISTFASSATLYDIGFNYFFQAPTDEHGGDLVFFQGHASPGVYA
RAFLEGRISEEQLENFRQEVDGNGLSSYPHPWLMPDFWQFPTVSMGLGPIQAIYQARFMKYLESRGFIPAGK
QKVWCFMGDGECDEPESLGAISLAGREKLDNLIFVINCNLQRLDGPVRGNAKIIQELEGVFRGAEWNVNKVI
WGRFWDPLFAKDTAGLLQQRMDEVIDGEYQNYKAKDGAYVREHFFGARPELLEMVKDLSDEEIWKLNRGGHD
PYKVYAAYHQAVNHKGQPTVILAKTIKGYGTGSGEAKNIAHNVKKVDVDSLRAFRDKFDIPVKDADLEKLPF
YKPEEGSAEAKYLAERRAALGGFMPVRRQKSMSVPVPPLETLKAMLDGSGDREISTTMAFVRIISQLVKDKE
LGPRIVPIVPDEARTFGMEGMFRQLGIYSSVGQLYEPVDKDQVMFYREDKKGQILEEGINEAGAMSSWIAAG
TSYSTHNQPMLPFYIFYSMFGFQRIGDLAWAAGDSRAHGFLIGGTAGRTTLNGEGLQHEDGHSHLLASTIPN
CRTYDPTYAYELAVIIREGSRQMIEEQQDIFYYITVMNENYVQPAMPKGAEEGIIKGMYLLEEDKKEAAHHV
QLLGSGTILREVEEAAKLLRNDFGIGADVWSVPSFNELRRDGLAVERWNRLHPGQKPKQSYVEECLGGRRGP
VIASTDYMKLYAEQIRQWVPSKEYKVLGTDGFGRSDSRKKLRNFFEVDRHWVVLAALEALADRGDIEPKVVA
EAIAKYGIDPEKRNPLDC*
AnnotationID:115GeneID:105
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 09:09:00
Gene Name:aceA
Confidence Code:1
GeneProduct:pyruvate dehydrogenase, E1 component
Cell Localization:()
Synonyms:pdhE
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2609
Secondary Functional Category(ies):energy metabolism
EC Number:1.2.4.1
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN65970.1|AE016225_3 (AE016775) pyruvate dehydrogenase, E1 component [Pseudomonas putida
           KT2440]
          Length = 881

 Score = 1628 bits (4216), Expect = 0.0
 Identities = 785/882 (89%), Positives = 837/882 (94%), Gaps = 1/882 (0%)

>gb|AAO58433.1| (AE016874) pyruvate dehydrogenase, E1 component [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 881

 Score = 1612 bits (4174), Expect = 0.0
 Identities = 774/882 (87%), Positives = 837/882 (94%), Gaps = 1/882 (0%)

>gb|AAN53506.1|AE015490_7 (AE015490) pyruvate dehydrogenase complex, E1 component, pyruvate
           dehydrogenase [Shewanella oneidensis MR-1]
          Length = 888

 Score = 1210 bits (3131), Expect = 0.0
 Identities = 578/882 (65%), Positives = 713/882 (80%), Gaps = 5/882 (0%)








 
Structural Features:
COG2609, AceE, Pyruvate dehydrogenase complex, dehydrogenase (E1) component 
[Energy production and conversion]
CD-Length = 887 residues,  99.4% aligned
Score = 1447 bits (3746), Expect = 0.0
Identities = 606/885 (68%), Positives = 718/885 (81%), Gaps = 6/885 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:213




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17355




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35082




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:105_cdd.html


    		




 
URL:

















    ReferenceID:35864




  
Author/Investigator(s):
        Rae JL, Cutfield JF, Lamont IL.





  
Title:
        Sequences and expression of pyruvate dehydrogenase genes from Pseudomonas aeruginosa.




  
PubMed:
        9171401


MedLine:
        




    
Source:
J Bacteriol. 1997 Jun;179(11):3561-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9171401









           
Homologs By Global Alignment 

           

       



     
Gene ID:105
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
203
                           PA5015_tr
                           translation of PA5015
                           882
                           99.99
                           99.99
                           0
                           4664.0
     

     
     
15059
                           gb|AAG08400.1|AE004914_1
                           (AE004914) pyruvate dehydrogenase [Pseudomonas aeruginosa PAO1]
                           882
                           99.99
                           99.99
                           0
                           4664.0
     

     
     
15060
                           gb|AAC45353.1|
                           (U47920) pyruvate dehydrogenase [Pseudomonas aeruginosa]
                           885
                           95.59
                           95.93
                           8
                           4390.0
     

     
     
15061
                           gb|AAN65970.1|AE016225_3
                           (AE016775) pyruvate dehydrogenase, E1 component [Pseudomonas putida KT2440]
                           882
                           89.00
                           94.89
                           1
                           4218.0
     

     
     
15062
                           gb|AAO58433.1|
                           (AE016874) pyruvate dehydrogenase, E1 component [Pseudomonas syringae pv. tomato str. DC3000]
                           882
                           87.75
                           94.89
                           1
                           4176.0
     

     






      

          
GC ORFID: 43107How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5015Glimmer Score: 

          
Start: 5171236Stop: 5173884

          
Length: 2649


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA5015 translation of PA5015Homolog Bit Score 1801.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3684 (ORF: BLASTX 1620.0), psyr_15may02_Scaffold16_revised_gene2649 (ORF: BLASTX 1134.0), t_PA5015 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCAAGACCTCGATCCCGTCGAAACCCAGGAATGGCTGGACGCCCTGGAGTCCGTACTCGACCGCGAAGGT 
GAAGACCGCGCTCATTACCTGATGACCCGCATGGGCGAGCTGGCCAGCCGTAGCGGCACCCAACTGCCCTAC 
GCGATCACCACGCCCTACCGCAACACCATTCCGGTAACCCACGAAGCACGCATGCCCGGCGACCTGTTCATG 
GAGCGCCGGATCCGCTCCCTGGTGCGCTGGAACGCCCTGGCGATGGTGATGCGGGCGAACAAGCACGACCCG 
GACCTGGGCGGCCACATCTCCACCTTCGCCTCCTCGGCGACCCTCTACGACATCGGCTTCAACTATTTCTTC 
CAGGCTCCCACCGACGAACATGGCGGCGACCTGGTGTTCTTCCAGGGCCACGCGTCCCCCGGCGTCTACGCC 
CGCGCCTTCCTCGAAGGCCGCATCAGCGAGGAACAACTGGAGAACTTCCGCCAGGAAGTGGACGGCAACGGC 
CTGTCCTCCTATCCGCACCCCTGGCTGATGCCGGACTTCTGGCAGTTCCCCACCGTATCCATGGGCCTTGGC 
CCGATCCAGGCGATCTACCAGGCACGCTTCATGAAGTACCTGGAAAGCCGCGGCTTCATCCCCGCCGGCAAG 
CAGAAGGTCTGGTGCTTCATGGGCGACGGCGAGTGCGACGAGCCGGAATCCCTCGGCGCGATCTCCCTGGCC 
GGCCGCGAGAAACTCGACAACCTGATCTTCGTCATCAACTGCAACCTGCAGCGCCTCGACGGCCCGGTCCGC 
GGCAACGCCAAGATCATCCAGGAACTGGAAGGCGTGTTCCGCGGCGCCGAGTGGAACGTCAACAAGGTCATC 
TGGGGTCGCTTCTGGGACCCGCTGTTCGCCAAGGACACCGCCGGCCTGCTGCAGCAGCGCATGGACGAGGTC 
ATCGACGGCGAATACCAGAACTACAAGGCGAAAGACGGCGCCTACGTCCGCGAGCACTTCTTCGGCGCGCGT 
CCGGAACTGCTGGAAATGGTCAAGGACCTTTCCGACGAGGAAATCTGGAAGCTCAACCGCGGCGGCCACGAC 
CCCTACAAGGTCTATGCGGCCTACCACCAGGCGGTCAACCACAAGGGCCAGCCGACCGTCATCCTGGCCAAG 
ACCATCAAGGGCTACGGCACCGGCAGCGGCGAAGCGAAGAACATCGCGCACAACGTGAAGAAGGTCGACGTC 
GACAGCCTGCGCGCGTTCCGCGACAAGTTCGACATCCCGGTCAAGGACGCCGACCTGGAGAAGCTGCCGTTC 
TACAAGCCTGAAGAAGGCAGCGCCGAGGCCAAGTACCTGGCCGAGCGCCGCGCCGCGCTGGGCGGCTTCATG 
CCGGTGCGCCGGCAGAAGAGCATGAGCGTGCCGGTTCCGCCGCTGGAAACCCTGAAGGCCATGCTCGACGGC 
TCCGGCGATCGCGAGATCTCCACCACCATGGCCTTCGTGCGGATCATCTCGCAGCTGGTCAAGGACAAGGAA 
CTCGGCCCGCGCATCGTCCCGATCGTCCCGGACGAGGCCCGTACCTTCGGCATGGAAGGCATGTTCCGCCAG 
CTCGGCATCTACTCCTCGGTCGGCCAGCTCTACGAGCCGGTGGACAAGGACCAGGTGATGTTCTACCGCGAG 
GACAAGAAGGGCCAGAtccTCGAGGAAGGCATCAACGAGGCCGGCGCCATGTCCAGCTGGATTGCCGCCGGT 
ACCTCCTACAGCACCCACAACCAGCCGATGCTGCCGTTCTACATCTTCTATTCGATGTTCGGCTTCCAGCGC 
ATCGGCGACCTGGCCTGGGCCGCCGGCGACAGCCGCGCGCACGGCTTCCTGATCGGCGGCACCGCCGGTCGC 
ACCACGCTGAACGGCGAAGGCCTGCAGCACGAGGACGGCCACAGCCACCTGCTGGCCTCGACCATCCCGAAC 
TGCCGCACCTACGATCCGACCTACGCCTACGAACTGGCGGTGATCATCCGCGAAGGCTCGCGGCAGATGATC 
GAAGAGCAGCAGGACATCTTCTACTACATCACCGTGATGAACGAGAACTACGTCCAGCCGGCCATGCCGAAG 
GGCGCAGAGGAAGGCATCATCAAGGGCATGTACCTCCTCGAGGAGGACAAGAAGGAAGCCGCTCACCACGTG 
CAACTGCTGGGCTCCGGCACCATCCTGCGCGAAGTCGAGGAAGCGGCCAAGCTGCTGCGCAACGACTTCGGT 
ATCGGCGCCGACGTCTGGAGCGTGCCGAGCTTCAACGAGCTGCGTCGCGACGGCCTTGCCGTCGAGCGCTGG 
AACCGCCTGCATCCGGGCCAGAAGCCGAAGCAGAGCTACGTCGAGGAGTGCCTGGGCGGCCGTCGCGGCCCG 
GTGATCGCCTCCACCGACTACATGAAGCTCTACGCCGAGCAGATCCGCCAGTGGGTTCCCTCCAAGGAGTAC 
AAGGTGCTCGGCACCGACGGCTTCGGCCGCAGCGACAGCCGCAAGAAGCTGCGCAACTTCTTCGAAGTGGAT 
CGTCACTGGGTGGTGCTGGCCGCGCTCGAAGCGCTGGCCGATCGCGGCGATATCGAGCCGAAAGTGGTGGCC 
GAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAA