ViewGene
Locus Name:
GeneID:
Location:

GeneID:1049
TrivialName: PA14_65130
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_65130
PAO1 Ortholog Locus:PA4931
Sequence Length:1395
Protein Length:464
Start: 5806932
Stop: 5805538
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08316.1|AE004906_4 (AE004906) replicative DNA helicase [Pseudomonas aeruginosa PAO1]
Identities = 462/464 (99%), Positives = 464/464 (100%)
Sequence:
ATGAACGAGATCACCAGCCCCGAGCAATACGACCTGGAAACCACAGCCCTGAAGGTGCCGCCGCACTCGATC
GAGGCCGAGCAGGCCGTGCTGGGAGGCCTGATGCTGGACAACAACGCCTGGGAGCGGGTGTCGGACTCGGTG
TCCGACGGCGATTTCTACCGCCATGACCATCGCCTGATCTTCCGCGCCATCTACAAGCTGGCCGAGGCCAAC
CAGCCGATCGACGTGGTGACCCTCTCCGAGCAGTTGGAGAAGGAAGGACAACTGGCCCAGGTCGGCGGCCTG
GCCTATCTCGGCGAACTGGCGAAGAACATTCCGTCGGTCGCCAACATCAAGGCCTACGCGCAGATCATCCGC
GAGCGCGCCACCCTGCGCCAACTGATCGGCATCAGCAACGAGATCGCCGACAGCGCCTTCCACCCGGAAGGG
CGCGGCGCCAACGAGATCCTCGACGAGGCCGAGCGGAAGATCTTCGAGATCGCCGAAGCGCGACCGAAGACC
GGCGGGCCGGTGGGGATCAGCGATATCCTGGTCAAGACCATCGACCGCATCGACTACCTGTTCAACACCAGC
GAGGCGCTGACCGGGGTGTCCACCGGTTTCACCGACCTCGACGAGAAGACCAGCGGCCTGCAGCCGGCCGAC
CTGATCATCGTCGCCGGGCGTCCGTCGATGGGTAAGACCACCTTCGCCATGAACCTGGTCGAGAATGCGGTG
ATGCGCACCGACAAGGCCGTGTTGGTGTACTCCCTGGAGATGCCCTCCGACTCGATCGTGATGCGTATGCTC
TCGTCGCTCGGTCGCATCGATCAGACCAAGGTCCGTTCCGGCAAGCTCGACGACGAGGATTGGCCGCGCCTG
ACCTCGGCGATCAACCTGCTCAACGACAAGAAGCTGTTCATCGACGACACCGCCGGCATCAGCCCCTCGGAA
ATGCGTGCGCGGACCCGTCGCCTGGTGCGCGAGCACGGCGACCTGGCGCTGATCATGATCGACTACCTGCAG
CTGATGCAGATACCCGGCTCCAGCGGCGACAACCGGACCAACGAGATTTCCGAGATCTCCCGCTCGCTGAAG
GCCCTGGCGAAGGAATTCAACTGCCCGGTGATCGCCCTGTCGCAGCTCAACCGCTCCCTGGAGCAGCGACCC
AACAAGCGCCCGGTGAACTCCGACTTGCGCGAATCCGGGGCGATCGAGCAGGACGCCGACGTGATCATGTTC
GTCTACCGCGACGAGGTGTACCACCCGGAGACCGAGTTCAAGGGCGTCGCCGAAATCATCATCGGCAAGCAG
CGTAACGGTCCGATCGGCACGGTGCGCCTGGCCTTCATCGGCAAATACACGCGCTTCGAGAACCTTGCCGCC
GGCATGTACAACTTCGAGGACGAATGA
Translation:
MNEITSPEQYDLETTALKVPPHSIEAEQAVLGGLMLDNNAWERVSDSVSDGDFYRHDHRLIFRAIYKLAEAN
QPIDVVTLSEQLEKEGQLAQVGGLAYLGELAKNIPSVANIKAYAQIIRERATLRQLIGISNEIADSAFHPEG
RGANEILDEAERKIFEIAEARPKTGGPVGISDILVKTIDRIDYLFNTSEALTGVSTGFTDLDEKTSGLQPAD
LIIVAGRPSMGKTTFAMNLVENAVMRTDKAVLVYSLEMPSDSIVMRMLSSLGRIDQTKVRSGKLDDEDWPRL
TSAINLLNDKKLFIDDTAGISPSEMRARTRRLVREHGDLALIMIDYLQLMQIPGSSGDNRTNEISEISRSLK
ALAKEFNCPVIALSQLNRSLEQRPNKRPVNSDLRESGAIEQDADVIMFVYRDEVYHPETEFKGVAEIIIGKQ
RNGPIGTVRLAFIGKYTRFENLAAGMYNFEDE*
AnnotationID:1058GeneID:1049
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 07:07:37
Gene Name:dnaB
Confidence Code:2
GeneProduct:replicative DNA helicase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(11) DNA replication, recombination, modification and repair
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0305,cd00984,pfam03796
Secondary Functional Category(ies):
EC Number:3.6.1.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58357.1| (AE016873) replicative DNA helicase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 464

 Score =  807 bits (2084), Expect = 0.0
 Identities = 400/464 (86%), Positives = 437/464 (94%)

>gb|AAN70442.1|AE016685_7 (AE016792) replicative DNA helicase [Pseudomonas putida KT2440]
          Length = 465

 Score =  795 bits (2054), Expect = 0.0
 Identities = 397/464 (85%), Positives = 435/464 (93%)

>gb|AAF93544.1| (AE004125) replicative DNA helicase [Vibrio cholerae]
          Length = 468

 Score =  572 bits (1474), Expect = e-162
 Identities = 283/455 (62%), Positives = 367/455 (80%), Gaps = 4/455 (0%)






 
Structural Features:
COG0305, DnaB, Replicative DNA helicase [DNA replication, recombination, and repair]
CD-Length = 435 residues,  99.8% aligned
Score =  529 bits (1364), Expect = 3e-151
Identities = 229/438 (52%), Positives = 309/438 (70%), Gaps = 4/438 (0%)

cd00984, DnaB_C, DnaB helicase C terminal domain.
CD-Length = 242 residues,  99.2% aligned
Score =  349 bits (896), Expect = 6e-97

pfam03796, DnaB_C, DnaB-like helicase C terminal domain. 
CD-Length = 198 residues,  99.5% aligned
Score =  294 bits (754), Expect = 2e-80
Identities = 128/199 (64%), Positives = 154/199 (77%), Gaps = 2/199 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2101




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19243




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35121




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1049_cdd.html


    		




 
URL:

















    ReferenceID:35920




  
Author/Investigator(s):
        Dreiseikelmann B, Riedel HD, Schuster H.





  
Title:
        A dnaB-like protein of Pseudomonas aeruginosa.




  
PubMed:
        3029680


MedLine:
        




    
Source:
Nucleic Acids Res. 1987 Jan 26;15(2):385-95.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3029680

















    ReferenceID:35921




  
Author/Investigator(s):
        Wong A, Kean L, Maurer R.





  
Title:
        Sequence of the dnaB gene of Salmonella typhimurium.




  
PubMed:
        2836367


MedLine:
        




    
Source:
J Bacteriol. 1988 Jun;170(6):2668-75.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2836367

















    ReferenceID:35922




  
Author/Investigator(s):
        Bruand C, Ehrlich SD.





  
Title:
        The Bacillus subtilis dnaI gene is part of the dnaB operon.




  
PubMed:
        7773414


MedLine:
        




    
Source:
Microbiology. 1995 May;141 ( Pt 5):1199-200.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7773414

















    ReferenceID:35923




  
Author/Investigator(s):
        Leipe DD, Aravind L, Grishin NV, Koonin EV.





  
Title:
        The bacterial replicative helicase DnaB evolved from a RecA duplication.




  
PubMed:
        10645945


MedLine:
        




    
Source:
Genome Res. 2000 Jan;10(1):5-16.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10645945









           
Homologs By Global Alignment 

           

       



     
Gene ID:1049
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1899
                           PA4931_tr
                           translation of PA4931
                           464
                           99.56
                           99.99
                           0
                           2330.0
     

     
     
65493
                           gb|AAG08316.1|AE004906_4
                           (AE004906) replicative DNA helicase [Pseudomonas aeruginosa PAO1]
                           464
                           99.56
                           99.99
                           0
                           2330.0
     

     
     
65494
                           gb|AAK00230.1|AF229443_1
                           (AF229443) replicative DNA helicase DnaB [Pseudomonas aeruginosa]
                           464
                           99.56
                           99.99
                           0
                           2330.0
     

     
     
65495
                           gb|AAO58357.1|
                           (AE016873) replicative DNA helicase [Pseudomonas syringae pv. tomato str. DC3000]
                           464
                           86.20
                           94.18
                           0
                           2084.0
     

     
     
65496
                           emb|CAD13464.1|
                           (AJ421512) replicative helicase dnaB2 [Pseudomonas sp. SLT2001]
                           464
                           85.77
                           93.53
                           0
                           2070.0
     

     






      

          
GC ORFID: 44051How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4931Glimmer Score: 

          
Start: 5070613Stop: 5069219

          
Length: 1395


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4931 translation of PA4931Homolog Bit Score 902.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3443 (ORF: BLASTX 807.0), psyr_15may02_Scaffold10_revised_gene1568 (ORF: BLASTX 483.0), t_PA4931 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACGAGATCACCAGCCCCGAGCAATACGACCTGGAAACCACAGCCCTGAAGGTGCCGCCGCACTCGATC 
GAGGCCGAGCAGGCCGTGCTGGGAGGCCTGATGCTGGACAACAACGCCTGGGAGCGGGTGTCGGACTCGGTG 
TCCGACGGCGATTTCTACCGCCATGACCATCGCCTGATCTTCCGCGCCATCTACAAGCTGGCCGAGGCCAAC 
CAGCCGATCGACGTGGTGACCCTCTCCGAGCAGTTGGAGAAGGAAGGACAACTGGCCCAGGTCGGCGGCCTG 
GCCTATCTCGGCGAACTGGCGAAGAACATTCCGTCGGTCGCCAACATCAAGGCCTACGCGCAGATCATCCGC 
GAGCGCGCCACCCTGCGCCAACTGATCGGCATCAGCAACGAGATCGCCGACAGCGCCTTCCACCCGGAAGGG 
CGCGGCGCCAACGAGATCCTCGACGAGGCCGAGCGGAAGATCTTCGAGATCGCCGAAGCGCGACCGAAGACC 
GGCGGGCCGGTGGGGATCAGCGATATCCTGGTCAAGACCATCGACCGCATCGACTACCTGTTCAACACCAGC 
GAGGCGCTGACCGGGGTGTCCACCGGTTTCACCGACCTCGACGAGAAGACCAGCGGCCTGCAGCCGGCCGAC 
CTGATCATCGTCGCCGGGCGTCCGTCGATGGGTAAGACCACCTTCGCCATGAACCTGGTCGAGAATGCGGTG 
ATGCGCACCGACAAGGCCGTGTTGGTGTACTCCCTGGAGATGCCCTCCGACTCGATCGTGATGCGTATGCTC 
TCGTCGCTCGGTCGCATCGATCAGACCAAGGTCCGTTCCGGCAAGCTCGACGACGAGGATTGGCCGCGCCTG 
ACCTCGGCGATCAACCTGCTCAACGACAAGAAGCTGTTCATCGACGACACCGCCGGCATCAGCCCCTCGGAA 
ATGCGTGCGCGGACCCGTCGCCTGGTGCGCGAGCACGGCGACCTGGCGCTGATCATGATCGACTACCTGCAG 
CTGATGCAGATACCCGGCTCCAGCGGCGACAACCGGACCAACGAGATTTCCGAGATCTCCCGCTCGCTGAAG 
GCCCTGGCGAAGGAATTCAACTGCCCGGTGATCGCCCTGTCGCAGCTCAACCGCTCCCTGGAGCAGCGACCC 
AACAAGCGCCCGGTGAACTCCGACTTGCGCGAATCCGGGGCGATCGAGCAGGACGCCGACGTGATCATGTTC 
GTCTACCGCGACGAGGTGTACCACCCGGAGACCGAGTTCAAGGGCGTCGCCGAAATCATCATCGGCAAGCAG 
CGTAACGGTCCGATCGGCACGGTGCGCCTGGCCTTCATCGGCAAATACACGCGCTTCGAGAACCTTGCCGCC 
GGCATGTACAACTTCGAGGACGAATGA