ViewGene
Locus Name:
GeneID:
Location:

GeneID:1040
TrivialName: PA14_69000
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_69000
PAO1 Ortholog Locus:PA5224
Sequence Length:1335
Protein Length:444
Start: 6155226
Stop: 6153892
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08609.1|AE004935_6 (AE004935) aminopeptidase P [Pseudomonas aeruginosa PAO1]
Identities = 443/444 (99%), Positives = 444/444 (100%)
Sequence:
ATGATCCGTATCCCCAAGTCGGAGTACGCCCGTCGGCGCAAGGCGCTGATGGCGCAGATGGAACCCAACAGC
ATCGCTATCCTGCCGGCGGCGCCGATGTACATCCGCAATCGCGACGTCGAGCATGTCTACCGCCAGGACAGC
GACTTCCAGTACCTCACCGGCTTCCCCGAGCCGGAGGCGGTGATGGCGCTGATTCCCGGCCGTGCCCACGGC
GAATACGTGCTGTTCTGCCGCGAGCGGGATCCGGAGCGTGAACTGTGGGACGGCCTGCGCGCCGGCCAGGAC
GGTGCGATCGGCCAGTACGGCGCCGACGACGCCTTTCCCATCGGCGATATCGACGACATCCTGCCCGGCCTG
ATCGAAGGCCGCGACCGGGTCTACTACGCCCTTGGCGCCAATCCCGACTTCGACCGCCGGCTGATGGACTGG
ATCAACGTGATCCGCTCCAAGGCCCGCCAGGGCGCGCAGCCGCCGAACGAGTTCGTTGCCCTCGACCACCTG
CTGCACGACCAGCGGCTGTACAAGAGCGCCAACGAAGTGAAGGTCATGCGCTACGCCGCCGAGGTCTCCGCC
CGGGCGCACATCCGGGCGATGGAGGTGTGCCGGCCGGGGCTGTTCGAGTACCACCTGGAAGCCGAGCTGGAA
TACGAGTTCCGCAAGGGCGGGGCGAAGATGCCGGCCTACGGCTCGATCGTCGCCGCCGGACGCAACGCCTGC
ATCCTGCACTACCGCGAGAACGATGCGGCGATCAAGGACGGCGACCTGATCCTGATCGATGCCGGCTGCGAG
ATCGACTGCTACGCCAGCGACATCACCCGTACCTTCCCCGCCAATGGCCGCTTCAGCCCCGAGCAGAAGGCG
ATCTACGAACTGGTGCTGGAAGCCAACATGGCGGCGTTCGACTACATCGCCCCCGGTCGCCACTGGAACGAG
GCCCATGAGGCCACCGTACGGGTCATCACCGCCGGGCTGGTGCGCCTGGGCCTGCTGGAGGGCGACGTCGAC
GAGCTGATCGCCCACGAGGCCTACAAGGCTTTCTACATGCACCGCGCCGGCCACTGGCTGGGGATGGACGTG
CACGACGTCGGCGAGTACCGCGTCGGCGGCGAATGGCGGGTGCTCGAACCGGGCATGGCGATGACCGTCGAG
CCGGGCATCTACATCGCCCCGGACAATACCTCGGTGGCGAAGAAGTGGCGCGGTATCGGCGTACGCATCGAG
GACGACGTGGTGGTCACCCGCAATGGCTGCGAAGTGCTGACCAACGGCGTGCCGAAGACCGTCGCCGAGATC
GAGGCGCTGATGGCCGCGGCCAAGAGCGAGGCAGCCTGA
Translation:
MIRIPKSEYARRRKALMAQMEPNSIAILPAAPMYIRNRDVEHVYRQDSDFQYLTGFPEPEAVMALIPGRAHG
EYVLFCRERDPERELWDGLRAGQDGAIGQYGADDAFPIGDIDDILPGLIEGRDRVYYALGANPDFDRRLMDW
INVIRSKARQGAQPPNEFVALDHLLHDQRLYKSANEVKVMRYAAEVSARAHIRAMEVCRPGLFEYHLEAELE
YEFRKGGAKMPAYGSIVAAGRNACILHYRENDAAIKDGDLILIDAGCEIDCYASDITRTFPANGRFSPEQKA
IYELVLEANMAAFDYIAPGRHWNEAHEATVRVITAGLVRLGLLEGDVDELIAHEAYKAFYMHRAGHWLGMDV
HDVGEYRVGGEWRVLEPGMAMTVEPGIYIAPDNTSVAKKWRGIGVRIEDDVVVTRNGCEVLTNGVPKTVAEI
EALMAAAKSEAA*
AnnotationID:1049GeneID:1040
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 13:01:01
Gene Name:pepP
Confidence Code:2
GeneProduct:aminopeptidase P
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:Xaa-Pro aminopeptidase
Functional Category Confidence Code:5
COGs:cd01087,COG0006,pfam05195
Secondary Functional Category(ies):amino acid transport and metabolism
EC Number:3.4.11.9
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70765.1|AE016720_8 (AE016793) aminopeptidase P II [Pseudomonas putida KT2440]
          Length = 444

 Score =  768 bits (1984), Expect = 0.0
 Identities = 371/444 (83%), Positives = 405/444 (91%)

>gb|AAO58649.1| (AE016875) Xaa-Pro aminopeptidase [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 444

 Score =  755 bits (1950), Expect = 0.0
 Identities = 361/444 (81%), Positives = 403/444 (90%)

>gb|AAM42529.1| (AE012443) aminopeptidase P [Xanthomonas campestris pv. campestris
           str. ATCC 33913]
          Length = 444

 Score =  526 bits (1355), Expect = e-148
 Identities = 249/439 (56%), Positives = 323/439 (73%), Gaps = 1/439 (0%)






 
Structural Features:
cd01087, Prolidase, Prolidase. E.C. 3.4.13.9. Also known as Xaa-Pro dipeptidase
CD-Length = 243 residues, 100.0% aligned
Score =  339 bits (872), Expect = 3e-94

COG0006, PepP, Xaa-Pro aminopeptidase [Amino acid transport and metabolism]
CD-Length = 384 residues,  99.7% aligned
Score =  272 bits (696), Expect = 6e-74
Identities = 147/424 (34%), Positives = 198/424 (46%), Gaps = 46/424 (10%)

pfam05195, AMP_N, Aminopeptidase P, N-terminal domain. 
CD-Length = 135 residues, 100.0% aligned
Score =  187 bits (476), Expect = 2e-48
Identities = 71/135 (52%), Positives = 94/135 (69%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2083




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19225




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34873




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1040_cdd.html


    		




 
URL:

















    ReferenceID:35754




  
Author/Investigator(s):
        Matos J, Nardi M, Kumura H, Monnet V.





  
Title:
        Genetic characterization of pepP, which encodes an aminopeptidase P whose deficiency does not affect Lactococcus lactis growth in milk, unlike deficiency of the X-prolyl dipeptidyl aminopeptidase.




  
PubMed:
        9797327


MedLine:
        




    
Source:
Appl Environ Microbiol. 1998 Nov;64(11):4591-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9797327

















    ReferenceID:35755




  
Author/Investigator(s):
        Butler MJ, Bergeron A, Soostmeyer G, Zimny T, Malek LT.





  
Title:
        Cloning and characterisation of an aminopeptidase P-encoding gene from Streptomyces lividans.




  
PubMed:
        8422994


MedLine:
        




    
Source:
Gene. 1993 Jan 15;123(1):115-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8422994









           
Homologs By Global Alignment 

           

       



     
Gene ID:1040
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1873
                           PA5224_tr
                           translation of PA5224
                           444
                           99.77
                           99.99
                           0
                           2335.0
     

     
     
64816
                           gb|AAG08609.1|AE004935_6
                           (AE004935) aminopeptidase P [Pseudomonas aeruginosa PAO1]
                           444
                           99.77
                           99.99
                           0
                           2335.0
     

     
     
64817
                           gb|AAN70765.1|AE016720_8
                           (AE016793) aminopeptidase P II [Pseudomonas putida KT2440]
                           444
                           83.55
                           91.21
                           0
                           1984.0
     

     
     
64818
                           gb|AAO58649.1|
                           (AE016875) Xaa-Pro aminopeptidase [Pseudomonas syringae pv. tomato str. DC3000]
                           444
                           81.30
                           90.76
                           0
                           1950.0
     

     
     
64819
                           gb|AAM42529.1|
                           (AE012443) aminopeptidase P [Xanthomonas campestris pv. campestris str. ATCC 33913]
                           447
                           55.70
                           72.48
                           6
                           1356.0
     

     






      

          
GC ORFID: 44042How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5224Glimmer Score: 

          
Start: 5418913Stop: 5417579

          
Length: 1335


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5224 translation of PA5224Homolog Bit Score 904.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3229 (ORF: BLASTX 756.0), t_PA5224 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATCCGTATCCCCAAGTCGGAGTACGCCCGTCGGCGCAAGGCGCTGATGGCGCAGATGGAACCCAACAGC 
ATCGCTATCCTGCCGGCGGCGCCGATGTACATCCGCAATCGCGACGTCGAGCATGTCTACCGCCAGGACAGC 
GACTTCCAGTACCTCACCGGCTTCCCCGAGCCGGAGGCGGTGATGGCGCTGATTCCCGGCCGTGCCCACGGC 
GAATACGTGCTGTTCTGCCGCGAGCGGGATCCGGAGCGTGAACTGTGGGACGGCCTGCGCGCCGGCCAGGAC 
GGTGCGATCGGCCAGTACGGCGCCGACGACGCCTTTCCCATCGGCGATATCGACGACATCCTGCCCGGCCTG 
ATCGAAGGCCGCGACCGGGTCTACTACGCCCTTGGCGCCAATCCCGACTTCGACCGCCGGCTGATGGACTGG 
ATCAACGTGATCCGCTCCAAGGCCCGCCAGGGCGCGCAGCCGCCGAACGAGTTCGTTGCCCTCGACCACCTG 
CTGCACGACCAGCGGCTGTACAAGAGCGCCAACGAAGTGAAGGTCATGCGCTACGCCGCCGAGGTCTCCGCC 
CGGGCGCACATCCGGGCGATGGAGGTGTGCCGGCCGGGGCTGTTCGAGTACCACCTGGAAGCCGAGCTGGAA 
TACGAGTTCCGCAAGGGCGGGGCGAAGATGCCGGCCTACGGCTCGATCGTCGCCGCCGGACGCAACGCCTGC 
ATCCTGCACTACCGCGAGAACGATGCGGCGATCAAGGACGGCGACCTGATCCTGATCGATGCCGGCTGCGAG 
ATCGACTGCTACGCCAGCGACATCACCCGTACCTTCCCCGCCAATGGCCGCTTCAGCCCCGAGCAGAAGGCG 
ATCTACGAACTGGTGCTGGAAGCCAACATGGCGGCGTTCGACTACATCGCCCCCGGTCGCCACTGGAACGAG 
GCCCATGAGGCCACCGTACGGGTCATCACCGCCGGGCTGGTGCGCCTGGGCCTGCTGGAGGGCGACGTCGAC 
GAGCTGATCGCCCACGAGGCCTACAAGGCTTTCTACATGCACCGCGCCGGCCACTGGCTGGGGATGGACGTG 
CACGACGTCGGCGAGTACCGCGTCGGCGGCGAATGGCGGGTGCTCGAACCGGGCATGGCGATGACCGTCGAG 
CCGGGCATCTACATCGCCCCGGACAATACCTCGGTGGCGAAGAAGTGGCGCGGTATCGGCGTACGCATCGAG 
GACGACGTGGTGGTCACCCGCAATGGCTGCGAAGTGCTGACCAACGGCGTGCCGAAGACCGTCGCCGAGATC 
GAGGCGCTGATGGCCGCGGCCAAGAGCGAGGCAGCCTGA