ViewGene
Locus Name:
GeneID:
Location:

GeneID:1036
TrivialName: PA14_01620
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_01620
PAO1 Ortholog Locus:PA0132
Sequence Length:1347
Protein Length:448
Start: 148687
Stop: 147341
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03522.1|AE004451_3 (AE004451) beta-alanine--pyruvate transaminase [Pseudomonas aeruginosa PAO1]
Identities = 447/448 (99%), Positives = 447/448 (99%)
Sequence:
ATGAACCAGCCGCTCAACGTGGCCCCGCCGGTTTCCAGCGAACTCAACCTGCGCGCCCACTGGATGCCCTTC
TCCGCCAACCGCAACTTCCAGAAGGACCCGCGGATCATCGTCGCCGCCGAAGGCAGCTGGCTGACCGACGAC
AAGGGCCGCAAGGTCTACGACAGCCTGTCCGGCCTGTGGACCTGCGGCGCCGGCCACTCGCGCAAGGAAATC
CAGGAGGCGGTGGCTCGCCAGCTCGGCACCCTCGACTACTCGCCGGGCTTCCAGTACGGCCATCCGCTGTCC
TTCCAGTTGGCCGAGAAGATCGCCGGGTTGCTGCCAGGCGAACTGAACCACGTGTTCTTCACCGGTTCCGGC
TCCGAGTGCGCCGACACCTCGATCAAGATGGCCCGCGCCTACTGGCGCCTGAAAGGCCAGCCGCAGAAGACC
AAGCTGATCGGCCGCGCCCGCGGCTACCACGGGGTCAACGTCGCCGGCACCAGCCTCGGCGGGATCGGCGGC
AACCGCAAGATGTTCGGCCAGTTGATGGACGTCGACCACCTGCCGCACACCCTGCAACCAGGCATGGCGTTC
ACCCGCGGGATGGCCCAGACCGGCGGCGTCGAGCTGGCCAACGAACTGCTCAAGCTGATCGAACTGCACGAC
GCCTCGAACATCGCCGCGGTGATCGTCGAGCCGATGTCCGGCTCCGCCGGCGTACTGGTACCGCCGGTCGGC
TACCTGCAGCGCCTGCGCGAGATCTGCGACCAGCACAACATCCTGCTGATCTTCGACGAGGTGATCACCGCC
TTCGGCCGCCTGGGCACCTACAGCGGCGCCGAGTACTTCGGCGTCACCCCGGACCTGATGAACGTCGCCAAG
CAGGTCACCAACGGCGCCGTGCCGATGGGCGCGGTGATCGCCAGCAGCGAGATCTACGACACCTTCATGAAC
CAGGCGCTGCCCGAGCACGCGGTGGAGTTCAGCCACGGCTACACCTACTCCGCGCACCCGGTCGCCTGCGCC
GCCGGCCTCGCCGCGCTGGACATCCTGGCCAGGGACAACCTGGTGCAGCAGTCCGCCGAACTGGCGCCGCAC
TTCGAGAAGGTCCTGCACGGCCTGCAAGGCGCGAAGAACGTCATCGACATCCGCAACTGCGGCCTGGCCGGC
GCGATCCAGATCGCCCCGCGCGACGGCGATCCGACCGTGCGTCCGTTCGAGGCCGGCATGAAGCTCTGGCAA
CAGGGTTTCTACGTGCGCTTCGGCGGCGATACCCTGCAATTCGGCCCGACCTTCAACGCCAGGCCGGAAGAG
CTGGACCGCCTGTTCGACGCGGTCGGCGAAGCGCTCAACGGCATCGCCTGA
Translation:
MNQPLNVAPPVSSELNLRAHWMPFSANRNFQKDPRIIVAAEGSWLTDDKGRKVYDSLSGLWTCGAGHSRKEI
QEAVARQLGTLDYSPGFQYGHPLSFQLAEKIAGLLPGELNHVFFTGSGSECADTSIKMARAYWRLKGQPQKT
KLIGRARGYHGVNVAGTSLGGIGGNRKMFGQLMDVDHLPHTLQPGMAFTRGMAQTGGVELANELLKLIELHD
ASNIAAVIVEPMSGSAGVLVPPVGYLQRLREICDQHNILLIFDEVITAFGRLGTYSGAEYFGVTPDLMNVAK
QVTNGAVPMGAVIASSEIYDTFMNQALPEHAVEFSHGYTYSAHPVACAAGLAALDILARDNLVQQSAELAPH
FEKVLHGLQGAKNVIDIRNCGLAGAIQIAPRDGDPTVRPFEAGMKLWQQGFYVRFGGDTLQFGPTFNARPEE
LDRLFDAVGEALNGIA*
AnnotationID:1045GeneID:1036
AnnotatorUID: diggins
Modification Date/Time: 2005-03-23 05:05:05
Gene Name:aptA
Confidence Code:2
GeneProduct:beta-alanine--pyruvate transaminase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:omega-amino acid:pyruvate transaminase
Functional Category Confidence Code:5
COGs:COG0161,pfam00202
Secondary Functional Category(ies):
EC Number:2.6.1.18
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO54316.1| (AE016858) beta-alanine--pyruvate aminotransferase [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 448

 Score =  731 bits (1886), Expect = 0.0
 Identities = 346/436 (79%), Positives = 392/436 (89%)

>gb|AAN66223.1|AE016250_5 (AE016776) beta-alanine--pyruvate transaminase [Pseudomonas putida
           KT2440]
          Length = 448

 Score =  721 bits (1862), Expect = 0.0
 Identities = 341/445 (76%), Positives = 394/445 (88%)

>dbj|BAB48961.1| (AP002997) beta-alanine-pyruvate transaminase [Mesorhizobium loti]
          Length = 442

 Score =  457 bits (1175), Expect = e-128
 Identities = 234/449 (52%), Positives = 302/449 (67%), Gaps = 10/449 (2%)






 
Structural Features:
COG0161, BioA, Adenosylmethionine-8-amino-7-oxononanoate aminotransferase 
[Coenzyme metabolism] 
CD-Length = 449 residues,  98.2% aligned
Score =  479 bits (1235), Expect = 3e-136
Identities = 186/447 (41%), Positives = 250/447 (55%), Gaps = 16/447 (3%)

pfam00202, Aminotran_3, Aminotransferase class-III.
CD-Length = 402 residues,  99.3% aligned
Score =  371 bits (954), Expect = 1e-103
Identities = 162/422 (38%), Positives = 221/422 (52%), Gaps = 28/422 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2075




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19217




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33506




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1036_cdd.html


    		




 
URL:

















    ReferenceID:35500




  
Author/Investigator(s):
        McCarn DF, Whitaker RA, Alam J, Vrba JM, Curtis SE.





  
Title:
        Genes encoding the alpha, gamma, delta, and four F0 subunits of ATP synthase constitute an operon in the cyanobacterium Anabaena sp. strain PCC 7120.




  
PubMed:
        2900236


MedLine:
        




    
Source:
J Bacteriol. 1988 Aug;170(8):3448-58.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2900236

















    ReferenceID:35501




  
Author/Investigator(s):
        Yun H, Lim S, Cho BK, Kim BG.





  
Title:
        omega-Amino acid:pyruvate transaminase from Alcaligenes denitrificans Y2k-2: a new catalyst for kinetic resolution of beta-amino acids and amines.




  
PubMed:
        15066855


MedLine:
        




    
Source:
Appl Environ Microbiol. 2004 Apr;70(4):2529-34.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=15066855









           
Homologs By Global Alignment 

           

       



     
Gene ID:1036
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1860
                           PA0132_tr
                           translation of PA0132
                           448
                           99.77
                           99.77
                           0
                           2340.0
     

     
     
64422
                           gb|AAG03522.1|AE004451_3
                           (AE004451) beta-alanine--pyruvate transaminase [Pseudomonas aeruginosa PAO1]
                           448
                           99.77
                           99.77
                           0
                           2340.0
     

     
     
64423
                           gb|AAO54316.1|
                           (AE016858) beta-alanine--pyruvate aminotransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           448
                           77.90
                           88.16
                           0
                           1879.0
     

     
     
64424
                           emb|CAE14553.1|
                           (BX571866) unnamed protein product [Photorhabdus luminescens subsp. laumondii TTO1]
                           448
                           76.56
                           86.83
                           5
                           1871.0
     

     
     
64425
                           gb|AAN66223.1|AE016250_5
                           (AE016776) beta-alanine--pyruvate transaminase [Pseudomonas putida KT2440]
                           448
                           76.33
                           88.16
                           0
                           1860.0
     

     






      

          
GC ORFID: 44038How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0132Glimmer Score: 

          
Start: 5950026Stop: 5948680

          
Length: 1347


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0132 translation of PA0132Homolog Bit Score 905.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3504 (ORF: BLASTX 733.0), t_PA5313 (ORF: BLASTX 420.0), t_PA0132 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACCAGCCGCTCAACGTGGCCCCGCCGGTTTCCAGCGAACTCAACCTGCGCGCCCACTGGATGCCCTTC 
TCCGCCAACCGCAACTTCCAGAAGGACCCGCGGATCATCGTCGCCGCCGAAGGCAGCTGGCTGACCGACGAC 
AAGGGCCGCAAGGTCTACGACAGCCTGTCCGGCCTGTGGACCTGCGGCGCCGGCCACTCGCGCAAGGAAATC 
CAGGAGGCGGTGGCTCGCCAGCTCGGCACCCTCGACTACTCGCCGGGCTTCCAGTACGGCCATCCGCTGTCC 
TTCCAGTTGGCCGAGAAGATCGCCGGGTTGCTGCCAGGCGAACTGAACCACGTGTTCTTCACCGGTTCCGGC 
TCCGAGTGCGCCGACACCTCGATCAAGATGGCCCGCGCCTACTGGCGCCTGAAAGGCCAGCCGCAGAAGACC 
AAGCTGATCGGCCGCGCCCGCGGCTACCACGGGGTCAACGTCGCCGGCACCAGCCTCGGCGGGATCGGCGGC 
AACCGCAAGATGTTCGGCCAGTTGATGGACGTCGACCACCTGCCGCACACCCTGCAACCAGGCATGGCGTTC 
ACCCGCGGGATGGCCCAGACCGGCGGCGTCGAGCTGGCCAACGAACTGCTCAAGCTGATCGAACTGCACGAC 
GCCTCGAACATCGCCGCGGTGATCGTCGAGCCGATGTCCGGCTCCGCCGGCGTACTGGTACCGCCGGTCGGC 
TACCTGCAGCGCCTGCGCGAGATCTGCGACCAGCACAACATCCTGCTGATCTTCGACGAGGTGATCACCGCC 
TTCGGCCGCCTGGGCACCTACAGCGGCGCCGAGTACTTCGGCGTCACCCCGGACCTGATGAACGTCGCCAAG 
CAGGTCACCAACGGCGCCGTGCCGATGGGCGCGGTGATCGCCAGCAGCGAGATCTACGACACCTTCATGAAC 
CAGGCGCTGCCCGAGCACGCGGTGGAGTTCAGCCACGGCTACACCTACTCCGCGCACCCGGTCGCCTGCGCC 
GCCGGCCTCGCCGCGCTGGACATCCTGGCCAGGGACAACCTGGTGCAGCAGTCCGCCGAACTGGCGCCGCAC 
TTCGAGAAGGTCCTGCACGGCCTGCAAGGCGCGAAGAACGTCATCGACATCCGCAACTGCGGCCTGGCCGGC 
GCGATCCAGATCGCCCCGCGCGACGGCGATCCGACCGTGCGTCCGTTCGAGGCCGGCATGAAGCTCTGGCAA 
CAGGGTTTCTACGTGCGCTTCGGCGGCGATACCCTGCAATTCGGCCCGACCTTCAACGCCAGGCCGGAAGAG 
CTGGACCGCCTGTTCGACGCGGTCGGCGAAGCGCTCAACGGCATCGCCTGA