ViewGene
Locus Name:
GeneID:
Location:

GeneID:103
TrivialName: PA14_57220
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_57220
PAO1 Ortholog Locus:PA4403
Sequence Length:2751
Protein Length:916
Start: 5100174
Stop: 5097424
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07791.1|AE004856_2 (AE004856) secretion protein SecA [Pseudomonas aeruginosa PAO1]
Identities = 915/916 (99%), Positives = 915/916 (99%)
Sequence:
ATGTTTGCGCCTTTGTTGAAGAAACTCTTTGGAAGCAAGAACGAGCGTGATGTAAAGCGCATGGCCAAGGCT
GTACAGGCCATCAATGCGCTCGAGCCCCAGATGGTGGCGCTCTCCGACGAGCAGTTGAAGGCGAAAACGGCT
GAATTCCAGCAGCGCTACGCCAAGGGCGAAACGCTCGACCAATTGCTGCCCGAAGCCTTCGCCGTGGTTCGC
GAGGCTGGCAAGCGGGTGATGGGCATGCGCCACTTCGACGTCCAGCTGATCGGCGGCATGACCCTGCACGAC
GGCAAGATCGCCGAAATGCGTACCGGCGAGGGCAAGACCCTGGTGGGCACCCTGCCGGTCTACCTCAACGCG
CTGTCCGGCAAGGGCGTGCACGTGGTCACGGTGAACGACTACCTGGCGCGCCGCGACGCCAACTGGATGCGC
CCGCTGTACGAGTTCCTCGGCCTCAGCGTCGGCGTGGTCACTCCCTTCCAGCCGCCGGAAGACAAGCGCGCC
GCCTACGCCGCCGATATCACCTACGGCACCAACAACGAATTCGGCTTCGATTACCTGCGCGACAACATGGCC
TTCAGCCTGGACGACAAGTTCCAGCGCGAGCTGAACTTCGCCGTGGTCGACGAAGTGGACTCGATCCTCATC
GACGAGGCGCGTACCCCGCTGATCATCTCCGGCCAGGCCGAAGACAGCTCCGAGCTGTACATCAAGATCAAC
AAGCTGATCCCGCGTCTCAATCGCCAGGTCGAAGAGGTCGAGGGCAAGCCGACCGAGGAAGGCCACTACAGC
ATCGACGAGAAGACCCGCCAGGTCGAACTCAACGAGCAGGGCCACCAGTTCATCGAGGACCTGCTGAGCCAG
AACGGCCTGCTCGGCGAAGGCGAGAGCCTCTATTCGGCGCACAACCTGAGCCTGCTGACCCACGTCTACGCA
GCGCTGCGCGCGCACACCCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGGCGACCAGATCCTCCTGATC
GACGAGCACACCGGCCGCACCATGCCTGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAGGCGAAG
GAGGGCCTGCCGATCCAGGCCGAGAGCCAGACCCTGGCCTCCACCACCTTCCAGAACTATTTCCGCCTGTAC
AACAAGCTGGCCGGCATGACCGGCACCGCCGATACCGAGGCCTTCGAGTTCCGCCAGATCTACGGTCTCGAC
GTGGTGGTGATCCCGACCCACCGGCCGATCGCGCGCAAGGACTTCAACGACCTGGTCTACCTGACCCAGGAA
GAGAAGTACGCGGCGATCATCACCGACATCAAGCAGTGCCAGGCCCTCGGCCGGCCGATCCTGGTCGGCACC
GCGTCGATCGAGAGTTCCGAGTACGTTTCCAAGCTGTTGCAAGAGGCAGGCATCGAGCACAAGGTGCTCAAC
GCCAAGTACCATGAGAAGGAAGCCGAGATCATCGCCCAGGCTGGCGCGCCCGGTTCGGTGACCATCGCCACC
AACATGGCCGGCCGCGGTACCGACATCCTCCTCGGTGGCAACTGGGAAGTCGAAGTCGCGGCCCTGGAGAAC
CCCACCGAGGAGCAGATCGCCCAGATCAAGGCCGAATGGCAGAAGCGCCACCAGCAGGTGATCGAGGCGGGT
GGCCTGCACGTGATCGCCTCCGAGCGCCACGAATCCCGCCGGATCGACAACCAGTTGCGCGGCCGTGCCGGT
CGTCAGGGCGACCCGGGTTCCAGCCGCTTCTACCTGTCGCTGGAAGACAACCTGATGCGGATCTTCGCCTCC
GACCGGGTGAAGAACTTCATGAAGGCGCTGGGCATGCAGTCCGGCGAGGCGATCGAGCACCGCATGGTGACC
AACGCCATCGAGAAGGCCCAGCGCAAGGTCGAAGGGCGCAACTTCGACATCCGCAAGCAACTGCTGGAATTC
GATGACGTAGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACACCCTGCTGTCGGCAGAGGACGTG
GGCGAGACCATCAAGGAGTTCCGCGAGGAAACCCTCAGCGCCACCATCAACCAACACATTCCGCCGCAGTCG
CTGCCCGAGCAGTGGGACATCGAAGGCCTGGAGGCCGCGCTGTACAGCGACTTCGCCGTGCGCCTGCCGATC
CAGCAGTGGCTGGACGAAGACGACAAGCTGTACGAGGAAACCCTGCGTAGCAAGATCCTCGAGCAGATCGTC
GCCGCCTACTACGAGAAGGAAGAGCTGGCCGGCGCCGAAGCCCTGCGCGCCTTCGAGAAGCAGATGCTGCTG
CGGGTACTGGACGACCTGTGGAAGGACCACCTGTCGACCATGGACCACCTGCGGCACGGCATCCACCTGCGT
GGCTACGCGCAGAAGAATCCGAAGCAGGAATACAAGCGCGAGTCCTTCACCCTGTTCCAGGAGTTGCTGGAC
TCGATCAAGCGCGACACCATCCGCGTGCTGTCCCACGTCCAGGTCCGTCGCGAAGACCCGGCCGAAGAGGAG
GCCCGCCTGCGTCGCGAGGCGGAGGAACTGGCCAAGCGCATGCAGTTCCAGCACGCCGAGGCTCCCTCGATG
GAGCAGGCCGTGGCCGGCGAGGAAGAAGAGCTTCCCGAGGGCCCGGCTCCGGTCGTGCCGCTCGAGCCTGTG
CGCAACGAGCAGAAGATCGGCCGCAACGAGCCTTGCCCGTGCGGTTCCGGCAAGAAATACAAGCACTGCCAC
GGGCAGCTGGACTAA
Translation:
MFAPLLKKLFGSKNERDVKRMAKAVQAINALEPQMVALSDEQLKAKTAEFQQRYAKGETLDQLLPEAFAVVR
EAGKRVMGMRHFDVQLIGGMTLHDGKIAEMRTGEGKTLVGTLPVYLNALSGKGVHVVTVNDYLARRDANWMR
PLYEFLGLSVGVVTPFQPPEDKRAAYAADITYGTNNEFGFDYLRDNMAFSLDDKFQRELNFAVVDEVDSILI
DEARTPLIISGQAEDSSELYIKINKLIPRLNRQVEEVEGKPTEEGHYSIDEKTRQVELNEQGHQFIEDLLSQ
NGLLGEGESLYSAHNLSLLTHVYAALRAHTLFHRNVEYIVQGDQILLIDEHTGRTMPGRRLSEGLHQAIEAK
EGLPIQAESQTLASTTFQNYFRLYNKLAGMTGTADTEAFEFRQIYGLDVVVIPTHRPIARKDFNDLVYLTQE
EKYAAIITDIKQCQALGRPILVGTASIESSEYVSKLLQEAGIEHKVLNAKYHEKEAEIIAQAGAPGSVTIAT
NMAGRGTDILLGGNWEVEVAALENPTEEQIAQIKAEWQKRHQQVIEAGGLHVIASERHESRRIDNQLRGRAG
RQGDPGSSRFYLSLEDNLMRIFASDRVKNFMKALGMQSGEAIEHRMVTNAIEKAQRKVEGRNFDIRKQLLEF
DDVANEQRKVIYHMRNTLLSAEDVGETIKEFREETLSATINQHIPPQSLPEQWDIEGLEAALYSDFAVRLPI
QQWLDEDDKLYEETLRSKILEQIVAAYYEKEELAGAEALRAFEKQMLLRVLDDLWKDHLSTMDHLRHGIHLR
GYAQKNPKQEYKRESFTLFQELLDSIKRDTIRVLSHVQVRREDPAEEEARLRREAEELAKRMQFQHAEAPSM
EQAVAGEEEELPEGPAPVVPLEPVRNEQKIGRNEPCPCGSGKKYKHCHGQLD*
AnnotationID:113GeneID:103
AnnotatorUID: diggins
Modification Date/Time: 2005-05-02 14:02:19
Gene Name:secA
Confidence Code:2
GeneProduct:preprotein translocase, SecA subunit
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(19) Protein secretion/export apparatus
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0653,pfam01043
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66968.1|AE016325_4 (AE016779) preprotein translocase, SecA subunit [Pseudomonas putida
           KT2440]
          Length = 939

 Score = 1546 bits (4002), Expect = 0.0
 Identities = 768/916 (83%), Positives = 843/916 (92%), Gaps = 5/916 (0%)


>gb|AAO57849.1| (AE016871) preprotein translocase, SecA subunit [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 913

 Score = 1535 bits (3974), Expect = 0.0
 Identities = 763/917 (83%), Positives = 846/917 (92%), Gaps = 5/917 (0%)








 
Structural Features:
COG0653, SecA, Preprotein translocase subunit SecA (ATPase, RNA helicase) 
[Intracellular trafficking and secretion]
CD-Length = 822 residues,  99.6% aligned
Score = 1107 bits (2865), Expect = 0.0
Identities = 538/905 (59%), Positives = 635/905 (70%), Gaps = 86/905 (9%)

pfam01043, SecA_protein, SecA protein, amino terminal region.
CD-Length = 415 residues, 100.0% aligned
Score =  655 bits (1692), Expect = 0.0
Identities = 267/433 (61%), Positives = 327/433 (75%), Gaps = 18/433 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:209




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17351




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:27979




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:103_cdd.html


    		




 
URL:

















    ReferenceID:32785




  
Author/Investigator(s):
        Guzzo J, Pages JM, Duong F, Lazdunski A, Murgier M.





  
Title:
        Pseudomonas aeruginosa alkaline protease: evidence for secretion genes and study of secretion mechanism.




  
PubMed:
        1832151


MedLine:
        




    
Source:
J Bacteriol. 1991 Sep;173(17):5290-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1832151

















    ReferenceID:32786




  
Author/Investigator(s):
        Douglas CM, Guidi-Rontani C, Collier RJ.





  
Title:
        Exotoxin A of Pseudomonas aeruginosa: active, cloned toxin is secreted into the periplasmic space of Escherichia coli.




  
PubMed:
        3117768


MedLine:
        




    
Source:
J Bacteriol. 1987 Nov;169(11):4962-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3117768









           
Homologs By Global Alignment 

           

       



     
Gene ID:103
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
201
                           PA4403_tr
                           translation of PA4403
                           916
                           99.89
                           99.89
                           0
                           4682.0
     

     
     
14879
                           gb|AAG07791.1|AE004856_2
                           (AE004856) secretion protein SecA [Pseudomonas aeruginosa PAO1]
                           916
                           99.89
                           99.89
                           0
                           4682.0
     

     
     
14880
                           gb|AAF26459.1|
                           (U19797) preprotein translocase [Pseudomonas aeruginosa]
                           916
                           99.89
                           99.89
                           0
                           4682.0
     

     
     
14882
                           gb|AAO57849.1|
                           (AE016871) preprotein translocase, SecA subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           917
                           83.20
                           92.25
                           5
                           3979.0
     

     
     
14881
                           gb|AAN66968.1|AE016325_4
                           (AE016779) preprotein translocase, SecA subunit [Pseudomonas putida KT2440]
                           944
                           81.35
                           89.30
                           33
                           4006.0
     

     






      

          
GC ORFID: 43105How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4403Glimmer Score: 

          
Start: 4363850Stop: 4361100

          
Length: 2751


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4403 translation of PA4403Homolog Bit Score 1808.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1136 (ORF: BLASTX 1538.0), t_PA4403 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTTTGCGCCTTTGTTGAAGAAACTCTTTGGAAGCAAGAACGAGCGTGATGTAAAGCGCATGGCCAAGGCT 
GTACAGGCCATCAATGCGCTCGAGCCCCAGATGGTGGCGCTCTCCGACGAGCAGTTGAAGGCGAAAACGGCT 
GAATTCCAGCAGCGCTACGCCAAGGGCGAAACGCTCGACCAATTGCTGCCCGAAGCCTTCGCCGTGGTTCGC 
GAGGCTGGCAAGCGGGTGATGGGCATGCGCCACTTCGACGTCCAGCTGATCGGCGGCATGACCCTGCACGAC 
GGCAAGATCGCCGAAATGCGTACCGGCGAGGGCAAGACCCTGGTGGGCACCCTGCCGGTCTACCTCAACGCG 
CTGTCCGGCAAGGGCGTGCACGTGGTCACGGTGAACGACTACCTGGCGCGCCGCGACGCCAACTGGATGCGC 
CCGCTGTACGAGTTCCTCGGCCTCAGCGTCGGCGTGGTCACTCCCTTCCAGCCGCCGGAAGACAAGCGCGCC 
GCCTACGCCGCCGATATCACCTACGGCACCAACAACGAATTCGGCTTCGATTACCTGCGCGACAACATGGCC 
TTCAGCCTGGACGACAAGTTCCAGCGCGAGCTGAACTTCGCCGTGGTCGACGAAGTGGACTCGATCCTCATC 
GACGAGGCGCGTACCCCGCTGATCATCTCCGGCCAGGCCGAAGACAGCTCCGAGCTGTACATCAAGATCAAC 
AAGCTGATCCCGCGTCTCAATCGCCAGGTCGAAGAGGTCGAGGGCAAGCCGACCGAGGAAGGCCACTACAGC 
ATCGACGAGAAGACCCGCCAGGTCGAACTCAACGAGCAGGGCCACCAGTTCATCGAGGACCTGCTGAGCCAG 
AACGGCCTGCTCGGCGAAGGCGAGAGCCTCTATTCGGCGCACAACCTGAGCCTGCTGACCCACGTCTACGCA 
GCGCTGCGCGCGCACACCCTGTTCCACCGCAACGTCGAGTACATCGTCCAGGGCGACCAGATCCTCCTGATC 
GACGAGCACACCGGCCGCACCATGCCTGGCCGTCGCCTGTCCGAGGGCCTGCACCAGGCCATCGAGGCGAAG 
GAGGGCCTGCCGATCCAGGCCGAGAGCCAGACCCTGGCCTCCACCACCTTCCAGAACTATTTCCGCCTGTAC 
AACAAGCTGGCCGGCATGACCGGCACCGCCGATACCGAGGCCTTCGAGTTCCGCCAGATCTACGGTCTCGAC 
GTGGTGGTGATCCCGACCCACCGGCCGATCGCGCGCAAGGACTTCAACGACCTGGTCTACCTGACCCAGGAA 
GAGAAGTACGCGGCGATCATCACCGACATCAAGCAGTGCCAGGCCCTCGGCCGGCCGATCCTGGTCGGCACC 
GCGTCGATCGAGAGTTCCGAGTACGTTTCCAAGCTGTTGCAAGAGGCAGGCATCGAGCACAAGGTGCTCAAC 
GCCAAGTACCATGAGAAGGAAGCCGAGATCATCGCCCAGGCTGGCGCGCCCGGTTCGGTGACCATCGCCACC 
AACATGGCCGGCCGCGGTACCGACATCCTCCTCGGTGGCAACTGGGAAGTCGAAGTCGCGGCCCTGGAGAAC 
CCCACCGAGGAGCAGATCGCCCAGATCAAGGCCGAATGGCAGAAGCGCCACCAGCAGGTGATCGAGGCGGGT 
GGCCTGCACGTGATCGCCTCCGAGCGCCACGAATCCCGCCGGATCGACAACCAGTTGCGCGGCCGTGCCGGT 
CGTCAGGGCGACCCGGGTTCCAGCCGCTTCTACCTGTCGCTGGAAGACAACCTGATGCGGATCTTCGCCTCC 
GACCGGGTGAAGAACTTCATGAAGGCGCTGGGCATGCAGTCCGGCGAGGCGATCGAGCACCGCATGGTGACC 
AACGCCATCGAGAAGGCCCAGCGCAAGGTCGAAGGGCGCAACTTCGACATCCGCAAGCAACTGCTGGAATTC 
GATGACGTAGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACACCCTGCTGTCGGCAGAGGACGTG 
GGCGAGACCATCAAGGAGTTCCGCGAGGAAACCCTCAGCGCCACCATCAACCAACACATTCCGCCGCAGTCG 
CTGCCCGAGCAGTGGGACATCGAAGGCCTGGAGGCCGCGCTGTACAGCGACTTCGCCGTGCGCCTGCCGATC 
CAGCAGTGGCTGGACGAAGACGACAAGCTGTACGAGGAAACCCTGCGTAGCAAGATCCTCGAGCAGATCGTC 
GCCGCCTACTACGAGAAGGAAGAGCTGGCCGGCGCCGAAGCCCTGCGCGCCTTCGAGAAGCAGATGCTGCTG 
CGGGTACTGGACGACCTGTGGAAGGACCACCTGTCGACCATGGACCACCTGCGGCACGGCATCCACCTGCGT 
GGCTACGCGCAGAAGAATCCGAAGCAGGAATACAAGCGCGAGTCCTTCACCCTGTTCCAGGAGTTGCTGGAC 
TCGATCAAGCGCGACACCATCCGCGTGCTGTCCCACGTCCAGGTCCGTCGCGAAGACCCGGCCGAAGAGGAG 
GCCCGCCTGCGTCGCGAGGCGGAGGAACTGGCCAAGCGCATGCAGTTCCAGCACGCCGAGGCTCCCTCGATG 
GAGCAGGCCGTGGCCGGCGAGGAAGAAGAGCTTCCCGAGGGCCCGGCTCCGGTCGTGCCGCTCGAGCCTGTG 
CGCAACGAGCAGAAGATCGGCCGCAACGAGCCTTGCCCGTGCGGTTCCGGCAAGAAATACAAGCACTGCCAC 
GGGCAGCTGGACTAA