ViewGene
Locus Name:
GeneID:
Location:

GeneID:1025
TrivialName: PA14_60820
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_60820
PAO1 Ortholog Locus:PA4597
Sequence Length:1440
Protein Length:479
Start: 5423541
Stop: 5422102
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07985.1|AE004873_7 (AE004873) Multidrug efflux outer membrane protein OprJ precursor [Pseudomonas aeruginosa PAO1]
Identities = 477/479 (99%), Positives = 478/479 (99%)
Sequence:
ATGCGCAAACCTGCATTCGGCGTATCGGCGTTGCTGATCGCCCTGACCCTCGGCGCCTGCTCCATGGCGCCG
ACCTACGAACGTCCCGCCGCGCCGGTGGCCGACAGCTGGAGCGGCGCCGCCGCCCAGCGCCAGGGCGCGGCG
ATCGACACGCTGGACTGGAAGAGTTTCATCGTCGATGCCGAACTACGCCGCCTGGTGGGCGTGGCCCTGGAT
AACAACCGCTCGCTGCGCCAGACCCTCCTGGATATCGAGGCGGCCCGCGCGCAGTACCGCATCCAGCGCGCC
GACCGGGTTCCGGGCCTGAATGCCGCTGCCACCGGCAACCGCCAGCGGCAGCCGGCCGACCTGTCCGCCGGC
AATCGCTCGGAAGTGGCCAGCAGCTACCAGGTCGGGCTGGCCCTGCCGGAGTACGAACTGGACCTCTTCGGT
CGGGTCAAGAGCCTGACCGACGCAGCCCTGCAACAGTACCTGGCCAGCGAGGAGGCAGCGCGCGCGGCACGG
ATCGCCCTGGTCGCCGAGGTCAGCCAGGCCTACCTGAGCTACGACGGCGCCCTGCGGCGCCTGGCGCTGACC
CGTCAGACCCTGGTCAGCCGCGAGTATTCCTTCGCCCTGATCGACCAGCGCCGCGCGGCCGGCGCCGCCACC
GCGCTGGACTACCAGGAAGCCCTTGGCCTGGTGGAGCAGGCGCGCGCCGAGCAGGAGCGCAACCTGCGGCAG
AAACAGCAGGCATTCAACGCGCTGGTGTTGCTGCTGGGCAGCGACGATGCCGCGCAGGCGATTCCGCGGAGT
CCGGGGCAGCGGCCGAAGCTGTTGCAGGACATCGCTCCCGGCACGCCGTCCGAGCTGATCGAGCGACGCCCC
GACATCCTTGCCGCCGAACATCGTTTGCGGGCGCGCAACGCGGATATCGGCGCGGCGCGCGCGGCGTTCTTC
CCACGCATCAGCCTGACCGGCAGCTTCGGCACCTCCAGCGCGGAAATGTCCGGCCTGTTCGATGGCGGCTCG
CGCTCCTGGAGCTTCCTGCCGACGCTGACGCTGCCGATCTTCGATGGCGGGCGCAACCGTGCCAACCTGAGC
CTGGCCGAGGCGCGCAAAGATTCGGCGGTGGCCGCCTACGAGGGGACCATCCAGACCGCTTTCCGCGAGGTC
GCCGACGCCCTGGCGGCCAGTGATACCCTGCGTCGCGAAGAGAAGGCCCTGCGCGCCCTGGCGAACAGCAGC
AACGAAGCCCTGAAGCTGGCCAAGGCACGCTACGAGAGTGGCGTCGACAACCACCTGCGCTACCTCGATGCG
CAGCGCAGCAGCTTCCTCAACGAGATCGCCTTCATCGACGGCAGCACCCAGCGGCAGATCGCCCTGGTCGAC
CTGTTCCGCGCGCTCGGCGGAGGCTGGGACGAGGGACGGAGCCTGGTGGTACATCGAGGCGGCAGGAGTTGA
Translation:
MRKPAFGVSALLIALTLGACSMAPTYERPAAPVADSWSGAAAQRQGAAIDTLDWKSFIVDAELRRLVGVALD
NNRSLRQTLLDIEAARAQYRIQRADRVPGLNAAATGNRQRQPADLSAGNRSEVASSYQVGLALPEYELDLFG
RVKSLTDAALQQYLASEEAARAARIALVAEVSQAYLSYDGALRRLALTRQTLVSREYSFALIDQRRAAGAAT
ALDYQEALGLVEQARAEQERNLRQKQQAFNALVLLLGSDDAAQAIPRSPGQRPKLLQDIAPGTPSELIERRP
DILAAEHRLRARNADIGAARAAFFPRISLTGSFGTSSAEMSGLFDGGSRSWSFLPTLTLPIFDGGRNRANLS
LAEARKDSAVAAYEGTIQTAFREVADALAASDTLRREEKALRALANSSNEALKLAKARYESGVDNHLRYLDA
QRSSFLNEIAFIDGSTQRQIALVDLFRALGGGWDEGRSLVVHRGGRS*
AnnotationID:1034GeneID:1025
AnnotatorUID: diggins
Modification Date/Time: 2005-05-06 06:06:26
Gene Name:oprJ
Confidence Code:1
GeneProduct:outer membrane protein OprJ
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(3) Antibiotic resistance and susceptibility
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1538,pfam02321
Secondary Functional Category(ies):transport of small molecules
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN68427.1|AE016475_6 (AE016784) outer membrane protein OprJ [Pseudomonas putida KT2440]
          Length = 468

 Score =  663 bits (1711), Expect = 0.0
 Identities = 344/458 (75%), Positives = 394/458 (86%), Gaps = 1/458 (0%)

>emb|CAD13537.1| (AL646057) PROBABLE DRUG EFFLUX LIPOPROTEIN [Ralstonia
           solanacearum]
          Length = 514

 Score =  353 bits (906), Expect = 1e-96
 Identities = 214/489 (43%), Positives = 287/489 (58%), Gaps = 14/489 (2%)






 
Structural Features:
COG1538, TolC, Outer membrane protein 
[Cell envelope biogenesis, outer membrane / 
Intracellular trafficking and secretion]
CD-Length = 457 residues,  98.0% aligned
Score =  236 bits (602), Expect = 1e-62
Identities = 200/454 (44%), Positives = 259/454 (57%), Gaps = 6/454 (1%)

pfam02321, OEP, Outer membrane efflux protein.
CD-Length = 188 residues,  97.9% aligned
Score =  114 bits (286), Expect = 5e-26
Identities = 70/184 (38%), Positives = 99/184 (53%), Gaps = 1/184 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2053




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19195




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31625




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1025_cdd.html


    		




 
URL:

















    ReferenceID:33289




  
Author/Investigator(s):
        Morita Y, Murata T, Mima T, Shiota S, Kuroda T, Mizushima T, Gotoh N, Nishino T, Tsuchiya T.





  
Title:
        Induction of mexCD-oprJ operon for a multidrug efflux pump by disinfectants in wild-type Pseudomonas aeruginosa PAO1.




  
PubMed:
        12654738


MedLine:
        




    
Source:
J Antimicrob Chemother. 2003 Apr;51(4):991-4. Epub 2003 Mar 13.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12654738

















    ReferenceID:33290




  
Author/Investigator(s):
        Okamoto K, Gotoh N, Nishino T.





  
Title:
        Extrusion of penem antibiotics by multicomponent efflux systems MexAB-OprM, MexCD-OprJ, and MexXY-OprM of Pseudomonas aeruginosa.




  
PubMed:
        12121960


MedLine:
        




    
Source:
Antimicrob Agents Chemother. 2002 Aug;46(8):2696-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12121960

















    ReferenceID:33291




  
Author/Investigator(s):
        Poole K.





  
Title:
        Multidrug efflux pumps and antimicrobial resistance in Pseudomonas aeruginosa and related organisms.




  
PubMed:
        11321581


MedLine:
        




    
Source:
J Mol Microbiol Biotechnol. 2001 Apr;3(2):255-64.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11321581

















    ReferenceID:33292




  
Author/Investigator(s):
        Gotoh N, Tsujimoto H, Tsuda M, Okamoto K, Nomura A, Wada T, Nakahashi M, Nishino T.





  
Title:
        Characterization of the MexC-MexD-OprJ multidrug efflux system in DeltamexA-mexB-oprM mutants of Pseudomonas aeruginosa.




  
PubMed:
        9687387


MedLine:
        




    
Source:
Antimicrob Agents Chemother. 1998 Aug;42(8):1938-43.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9687387

















    ReferenceID:33293




  
Author/Investigator(s):
        Srikumar R, Kon T, Gotoh N, Poole K.





  
Title:
        Expression of Pseudomonas aeruginosa multidrug efflux pumps MexA-MexB-OprM and MexC-MexD-OprJ in a multidrug-sensitive Escherichia coli strain.




  
PubMed:
        9449262


MedLine:
        




    
Source:
Antimicrob Agents Chemother. 1998 Jan;42(1):65-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9449262

















    ReferenceID:33294




  
Author/Investigator(s):
        Poole K, Gotoh N, Tsujimoto H, Zhao Q, Wada A, Yamasaki T, Neshat S, Yamagishi J, Li XZ, Nishino T.





  
Title:
        Overexpression of the mexC-mexD-oprJ efflux operon in nfxB-type multidrug-resistant strains of Pseudomonas aeruginosa.




  
PubMed:
        8878035


MedLine:
        




    
Source:
Mol Microbiol. 1996 Aug;21(4):713-24.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8878035

















    ReferenceID:33295




  
Author/Investigator(s):
        Masuda N, Gotoh N, Ohya S, Nishino T.





  
Title:
        Quantitative correlation between susceptibility and OprJ production in NfxB mutants of Pseudomonas aeruginosa.




  
PubMed:
        8849250


MedLine:
        




    
Source:
Antimicrob Agents Chemother. 1996 Apr;40(4):909-13.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8849250

















    ReferenceID:33296




  
Author/Investigator(s):
        Hosaka M, Gotoh N, Nishino T.





  
Title:
        Purification of a 54-kilodalton protein (OprJ) produced in NfxB mutants of Pseudomonas aeruginosa and production of a monoclonal antibody specific to OprJ.




  
PubMed:
        7486910


MedLine:
        




    
Source:
Antimicrob Agents Chemother. 1995 Aug;39(8):1731-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7486910









           
Homologs By Global Alignment 

           

       



     
Gene ID:1025
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1845
                           PA4597_tr
                           translation of PA4597
                           479
                           99.58
                           99.79
                           0
                           2349.0
     

     
     
63891
                           gb|AAG07985.1|AE004873_7
                           (AE004873) Multidrug efflux outer membrane protein OprJ precursor [Pseudomonas aeruginosa PAO1]
                           479
                           99.58
                           99.79
                           0
                           2349.0
     

     
     
63892
                           gb|AAB41958.1|
                           (U57969) outer membrane protein OprJ [Pseudomonas aeruginosa]
                           479
                           99.16
                           99.37
                           0
                           2327.0
     

     
     
63893
                           gb|AAN68427.1|AE016475_6
                           (AE016784) outer membrane protein OprJ [Pseudomonas putida KT2440]
                           480
                           72.08
                           82.70
                           13
                           1704.0
     

     
     
63894
                           emb|CAD24340.1|
                           (AJ431260) OprJ protein [uncultured bacterium]
                           484
                           65.08
                           78.51
                           14
                           1565.0
     

     






      

          
GC ORFID: 44027How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4597Glimmer Score: 

          
Start: 4687221Stop: 4685782

          
Length: 1440


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4597 translation of PA4597Homolog Bit Score 909.0

          
Other Homologs:  t_PA0427 (ORF: BLASTX 387.0), psyr_15may02_Scaffold5_revised_gene6179 (ORF: BLASTX 365.0), psyr_15may02_Scaffold1_revised_gene30 (ORF: BLASTX 364.0), psyr_15may02_Scaffold7_revised_gene7338 (ORF: BLASTX 344.0), t_PA4597 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGCAAACCTGCATTCGGCGTATCGGCGTTGCTGATCGCCCTGACCCTCGGCGCCTGCTCCATGGCGCCG 
ACCTACGAACGTCCCGCCGCGCCGGTGGCCGACAGCTGGAGCGGCGCCGCCGCCCAGCGCCAGGGCGCGGCG 
ATCGACACGCTGGACTGGAAGAGTTTCATCGTCGATGCCGAACTACGCCGCCTGGTGGGCGTGGCCCTGGAT 
AACAACCGCTCGCTGCGCCAGACCCTCCTGGATATCGAGGCGGCCCGCGCGCAGTACCGCATCCAGCGCGCC 
GACCGGGTTCCGGGCCTGAATGCCGCTGCCACCGGCAACCGCCAGCGGCAGCCGGCCGACCTGTCCGCCGGC 
AATCGCTCGGAAGTGGCCAGCAGCTACCAGGTCGGGCTGGCCCTGCCGGAGTACGAACTGGACCTCTTCGGT 
CGGGTCAAGAGCCTGACCGACGCAGCCCTGCAACAGTACCTGGCCAGCGAGGAGGCAGCGCGCGCGGCACGG 
ATCGCCCTGGTCGCCGAGGTCAGCCAGGCCTACCTGAGCTACGACGGCGCCCTGCGGCGCCTGGCGCTGACC 
CGTCAGACCCTGGTCAGCCGCGAGTATTCCTTCGCCCTGATCGACCAGCGCCGCGCGGCCGGCGCCGCCACC 
GCGCTGGACTACCAGGAAGCCCTTGGCCTGGTGGAGCAGGCGCGCGCCGAGCAGGAGCGCAACCTGCGGCAG 
AAACAGCAGGCATTCAACGCGCTGGTGTTGCTGCTGGGCAGCGACGATGCCGCGCAGGCGATTCCGCGGAGT 
CCGGGGCAGCGGCCGAAGCTGTTGCAGGACATCGCTCCCGGCACGCCGTCCGAGCTGATCGAGCGACGCCCC 
GACATCCTTGCCGCCGAACATCGTTTGCGGGCGCGCAACGCGGATATCGGCGCGGCGCGCGCGGCGTTCTTC 
CCACGCATCAGCCTGACCGGCAGCTTCGGCACCTCCAGCGCGGAAATGTCCGGCCTGTTCGATGGCGGCTCG 
CGCTCCTGGAGCTTCCTGCCGACGCTGACGCTGCCGATCTTCGATGGCGGGCGCAACCGTGCCAACCTGAGC 
CTGGCCGAGGCGCGCAAAGATTCGGCGGTGGCCGCCTACGAGGGGACCATCCAGACCGCTTTCCGCGAGGTC 
GCCGACGCCCTGGCGGCCAGTGATACCCTGCGTCGCGAAGAGAAGGCCCTGCGCGCCCTGGCGAACAGCAGC 
AACGAAGCCCTGAAGCTGGCCAAGGCACGCTACGAGAGTGGCGTCGACAACCACCTGCGCTACCTCGATGCG 
CAGCGCAGCAGCTTCCTCAACGAGATCGCCTTCATCGACGGCAGCACCCAGCGGCAGATCGCCCTGGTCGAC 
CTGTTCCGCGCGCTCGGCGGAGGCTGGGACGAGGGACGGAGCCTGGTGGTACATCGAGGCGGCAGGAGTTGA