ViewGene
Locus Name:
GeneID:
Location:

GeneID:102
TrivialName: PA14_44290
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_44290
PAO1 Ortholog Locus:PA1562
Sequence Length:2733
Protein Length:910
Start: 3940471
Stop: 3943203
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04951.1|AE004584_7 (AE004584) aconitate hydratase 1 [Pseudomonas aeruginosa PAO1]
Identities = 908/910 (99%), Positives = 910/910 (100%)
Sequence:
ATGCCTGCTTTGGACAGCCTGAAAACCCTTCGCAGTCTCGCTGTGGACGGCAAGACCTACCACTACTACAGC
CTCCCGGAAGCCGCCCGGACCCTGGGCGATCTCGGCAAGCTGCCGATGTCGCTGAAGGTCCTGCTGGAAAAC
CTCCTGCGCTGGGAGGACGGCAGCACCGTCACCGGCGACGACCTCAAGGCCCTCGCCGGCTGGCTCAGGGAG
CGCCGCTCCGACCGCGAGATCCAGTACCGCCCGGCGCGGGTCCTGATGCAGGACTTCACCGGGGTGCCCGCG
GTGGTCGACCTGGCCGCCATGCGCGCGGCCATGGCCAAGGCCGGCGGCGACCCGCAGAAGATCAACCCGCTG
TCGCCGGTGGACCTGGTCATCGACCACTCGGTGATGGTCGACAAGTTCGCCAGCGAATCGGCCTTCGAACAG
AACGTTGAGATCGAGATGCAGCGCAACGGCGAACGCTACGCCTTCCTGCGCTGGGGCCAGAACGCCTTCGAC
AATTTCAGCGTGGTACCGCCGGGCACCGGCATCTGCCACCAGGTCAACCTGGAGTACCTGGGCCGCACGGTC
TGGACCAAGGAAGAGGATGGCAGGACCTACGCCTTCCCCGACACCCTGGTCGGCACCGACTCCCACACCACC
ATGATCAACGGCCTCGGCGTGCTCGGCTGGGGCGTCGGCGGCATCGAGGCGGAAGCCGCCATGCTCGGCCAG
CCGGTATCGATGCTGATCCCCGAGGTCATCGGCTTCAAGCTCACCGGCAAGCTGCGCGAGGGCATCACCGCC
ACCGACCTGGTGCTGACCGTCACGCAGATGCTGCGCAAGAAGGGCGTGGTGGGGAAATTCGTCGAGTTCTAT
GGCGACGGCCTGGCCGACCTGCCCCTGGCGGACCGCGCCACCATCGCCAACATGGCCCCGGAATACGGCGCC
ACCTGCGGCTTCTTCCCGGTGGACGAGATCACCCTCGGCTACCTGCGCCTGTCCGGACGCCCGGAAAGCACG
GTCAAGCTGGTCGAGGCCTACAGCAAGGAACAGGGCCTGTGGCGCGAGAAGGGCCACGAGCCGGTGTTCACC
GACACCCTGCACCTGGACATGGGCGAAGTCGAGGCCAGCCTGGCCGGACCGAAGCGCCCGCAGGACCGCGTG
GCCCTGCAGAACGTCGCCAGCGCCTTCAACGAGTTCCTCGGCCTGCAACTGCATCCCTCCAGCACCGAAGAA
GGCCGCCTGCTCAGCGAGGGCGGCGGCGGCACCGCGGTCGGCGCCAACGCCGCCTTCGGCGAGATCGACTAC
CAGCACGACGGCCAGACCCACCGCCTGAAGAACGGCGCGGTGGTGATCGCCGCCATCACCTCCTGCACCAAC
ACCTCCAACCCCAGCGTGATGATGGCCGCCGGGCTGCTGGCGAAGAAGGCGGTGGAGAAAGGCCTGCAACGC
AAGCCCTGGGTGAAGAGTTCGCTGGCGCCGGGCTCCAAGGTGGTCACCGACTACTTCAAGGCCGCCGGCCTG
ACCCGCTACCTCGACGAACTCGGCTTCGACCTGGTCGGCTACGGCTGCACCACCTGCATCGGCAACTCCGGG
CCGCTGCTGGAGCCGATCGAGAAAGCCATCCAGCAGGCCGACCTGACCGTCGCCTCGGTACTCTCCGGCAAC
CGTAACTTCGAAGGCCGCGTGCACCCGTTGGTGAAGACCAACTGGCTGGCCTCGCCGCCGCTGGTGGTGGCC
TACGCCCTGGCCGGCAGCGTGCGGATCAACCTCAGCGAGGAACCGCTGGGCACCGGCAAGGACGGCCAGCCG
GTCTACCTGAAGGACATCTGGCCGAGCCAGAAGGAAATCGCCGAGGCGATCCAGAAGGTCGACACCGAGATG
TTCCACAAGGAGTACGCCGAGGTCTTCGCCGGCGACGAGAAATGGCAGGCGATCCAGGTGCCGCAGTCGGAC
ACCTACGAGTGGCAGGCCGACTCCACCTACATCCAGCATCCACCCTTCTTCGAACACATCGCCGAGGCGCCG
CCGGCCATCGCCGACGTCGAGCAGGCGCGGGTGCTGGCGGTGCTGGGCGACTCGGTGACCACCGACCACATC
TCCCCTGCCGGCAACATCAAGGCCGACAGCCCGGCCGGCCGCTACCTGCGCGAGCACGGCGTGGAACCGAAG
GACTTCAACTCCTACGGCTCCCGTCGCGGCAACCATGAGGTGATGATGCGCGGCACCTTCGCCAACATCCGC
ATCAAGAACGAGATGCTCGGCGGCGAGGAAGGCGGCAATACCCTGTACGTGCCCAGCGGCGAGAAACTGGCG
ATCTACGACGCGGCCATGCGCTACCAGCAGGACGGCACCCCGCTGGTGATCGTCGCCGGCAAGGAATACGGC
ACCGGCTCGTCGCGCGACTGGGCGGCCAAGGGTACCAACCTGCTGGGCGTGAAGGCGGTGATCGCGGAGAGC
TTCGAGCGCATCCACCGCTCCAACCTGGTCGGCATGGGCGTGCTGCCGTTGCAGTTCGAGAACGGCCAGGAC
CGCAAGAGCCTGAAGCTGACCGGCAAGGAGGTGCTGAACATCCGCGGCCTCGGCGGCGAACTCAAACCGCAC
ATGCCGCTCAGCGTGGAAGTGACCCGCGAGGACGGCAGCCAGGACAGCTTCAAGGTCCTCTGCCGCATCGAC
ACCCTCAACGAGGTCGAGTACTTCAAGGCCGGCGGCATCCTCCACTACGTGCTGCGCAGCATGCTCTGA
Translation:
MPALDSLKTLRSLAVDGKTYHYYSLPEAARTLGDLGKLPMSLKVLLENLLRWEDGSTVTGDDLKALAGWLRE
RRSDREIQYRPARVLMQDFTGVPAVVDLAAMRAAMAKAGGDPQKINPLSPVDLVIDHSVMVDKFASESAFEQ
NVEIEMQRNGERYAFLRWGQNAFDNFSVVPPGTGICHQVNLEYLGRTVWTKEEDGRTYAFPDTLVGTDSHTT
MINGLGVLGWGVGGIEAEAAMLGQPVSMLIPEVIGFKLTGKLREGITATDLVLTVTQMLRKKGVVGKFVEFY
GDGLADLPLADRATIANMAPEYGATCGFFPVDEITLGYLRLSGRPESTVKLVEAYSKEQGLWREKGHEPVFT
DTLHLDMGEVEASLAGPKRPQDRVALQNVASAFNEFLGLQLHPSSTEEGRLLSEGGGGTAVGANAAFGEIDY
QHDGQTHRLKNGAVVIAAITSCTNTSNPSVMMAAGLLAKKAVEKGLQRKPWVKSSLAPGSKVVTDYFKAAGL
TRYLDELGFDLVGYGCTTCIGNSGPLLEPIEKAIQQADLTVASVLSGNRNFEGRVHPLVKTNWLASPPLVVA
YALAGSVRINLSEEPLGTGKDGQPVYLKDIWPSQKEIAEAIQKVDTEMFHKEYAEVFAGDEKWQAIQVPQSD
TYEWQADSTYIQHPPFFEHIAEAPPAIADVEQARVLAVLGDSVTTDHISPAGNIKADSPAGRYLREHGVEPK
DFNSYGSRRGNHEVMMRGTFANIRIKNEMLGGEEGGNTLYVPSGEKLAIYDAAMRYQQDGTPLVIVAGKEYG
TGSSRDWAAKGTNLLGVKAVIAESFERIHRSNLVGMGVLPLQFENGQDRKSLKLTGKEVLNIRGLGGELKPH
MPLSVEVTREDGSQDSFKVLCRIDTLNEVEYFKAGGILHYVLRSML*
AnnotationID:112GeneID:102
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 06:06:26
Gene Name:acnA
Confidence Code:2
GeneProduct:aconitate hydratase 1
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1048,cd01586,pfam00330
Secondary Functional Category(ies):
EC Number:4.2.1.3
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55534.1| (AE016863) aconitate hydratase 1 [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 914

 Score = 1537 bits (3980), Expect = 0.0
 Identities = 762/912 (83%), Positives = 833/912 (91%), Gaps = 2/912 (0%)

>gb|AAN67725.1|AE016403_10 (AE016781) aconitate hydratase 1 [Pseudomonas putida KT2440]
          Length = 913

 Score = 1529 bits (3958), Expect = 0.0
 Identities = 755/911 (82%), Positives = 825/911 (90%), Gaps = 1/911 (0%)

>gb|AAL20630.1| (AE008776) aconitate hydratase 1 [Salmonella typhimurium LT2]
          Length = 891

 Score = 1266 bits (3275), Expect = 0.0
 Identities = 624/899 (69%), Positives = 734/899 (81%), Gaps = 20/899 (2%)






 
Structural Features:
COG1048, AcnA, Aconitase A [Energy production and conversion]
CD-Length = 861 residues,  99.9% aligned
Score = 1227 bits (3175), Expect = 0.0
Identities = 593/908 (65%), Positives = 684/908 (75%), Gaps = 50/908 (5%)

cd01586, AcnA_IRP, Aconitase A catalytic domain.
CD-Length = 484 residues, 100.0% aligned
Score =  728 bits (1882), Expect = 0.0

pfam00330, Aconitase, Aconitase family (aconitate hydratase).
CD-Length = 465 residues, 100.0% aligned
Score =  500 bits (1288), Expect = 4e-142
Identities = 217/525 (41%), Positives = 277/525 (52%), Gaps = 60/525 (11%)






   
Genomic Context:






   
Comment:


















    ReferenceID:207




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17349




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37235




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:102_cdd.html


    		




 
URL:

















    ReferenceID:37913




  
Author/Investigator(s):
        Thony-Meyer L, Kunzler P.





  
Title:
        The Bradyrhizobium japonicum aconitase gene (acnA) is important for free-living growth but not for an effective root nodule symbiosis.




  
PubMed:
        8892815


MedLine:
        




    
Source:
J Bacteriol. 1996 Nov;178(21):6166-72.

Erratum in:
    * J Bacteriol 1997 Mar;179(5):1836.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8892815

















    ReferenceID:37914




  
Author/Investigator(s):
        Prodromou C, Artymiuk PJ, Guest JR.





  
Title:
        The aconitase of Escherichia coli. Nucleotide sequence of the aconitase gene and amino acid sequence similarity with mitochondrial aconitases, the iron-responsive-element-binding protein and isopropylmalate isomerases.




  
PubMed:
        1541275


MedLine:
        




    
Source:
Eur J Biochem. 1992 Mar 1;204(2):599-609.

Erratum in:
    * Eur J Biochem 1992 Aug 1;207(3):1129.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=92174916&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:102
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
199
                           PA1562_tr
                           translation of PA1562
                           910
                           99.78
                           99.99
                           0
                           4710.0
     

     
     
14741
                           gb|AAG04951.1|AE004584_7
                           (AE004584) aconitate hydratase 1 [Pseudomonas aeruginosa PAO1]
                           910
                           99.78
                           99.99
                           0
                           4710.0
     

     
     
14742
                           gb|AAO55534.1|
                           (AE016863) aconitate hydratase 1 [Pseudomonas syringae pv. tomato str. DC3000]
                           914
                           83.36
                           91.13
                           4
                           3984.0
     

     
     
14743
                           gb|AAN67725.1|AE016403_10
                           (AE016781) aconitate hydratase 1 [Pseudomonas putida KT2440]
                           913
                           82.69
                           90.36
                           3
                           3960.0
     

     
     
14744
                           emb|CAE14806.1|
                           (BX571867) Aconitate hydratase 1 (citrate hydro-lyase 1) (aconitase 1) [Photorhabdus luminescens subsp. laumondii TTO1]
                           912
                           69.51
                           80.48
                           23
                           3296.0
     

     






      

          
GC ORFID: 43104How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1562Glimmer Score: 

          
Start: 1013438Stop: 1010706

          
Length: 2733


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1562 translation of PA1562Homolog Bit Score 1818.0

          
Other Homologs:  psyr_15may02_Scaffold8_revised_gene7517 (ORF: BLASTX 1541.0), psyr_15may02_Scaffold1_revised_gene1064 (ORF: BLASTX 701.0), t_PA1562 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCTGCTTTGGACAGCCTGAAAACCCTTCGCAGTCTCGCTGTGGACGGCAAGACCTACCACTACTACAGC 
CTCCCGGAAGCCGCCCGGACCCTGGGCGATCTCGGCAAGCTGCCGATGTCGCTGAAGGTCCTGCTGGAAAAC 
CTCCTGCGCTGGGAGGACGGCAGCACCGTCACCGGCGACGACCTCAAGGCCCTCGCCGGCTGGCTCAGGGAG 
CGCCGCTCCGACCGCGAGATCCAGTACCGCCCGGCGCGGGTCCTGATGCAGGACTTCACCGGGGTGCCCGCG 
GTGGTCGACCTGGCCGCCATGCGCGCGGCCATGGCCAAGGCCGGCGGCGACCCGCAGAAGATCAACCCGCTG 
TCGCCGGTGGACCTGGTCATCGACCACTCGGTGATGGTCGACAAGTTCGCCAGCGAATCGGCCTTCGAACAG 
AACGTTGAGATCGAGATGCAGCGCAACGGCGAACGCTACGCCTTCCTGCGCTGGGGCCAGAACGCCTTCGAC 
AATTTCAGCGTGGTACCGCCGGGCACCGGCATCTGCCACCAGGTCAACCTGGAGTACCTGGGCCGCACGGTC 
TGGACCAAGGAAGAGGATGGCAGGACCTACGCCTTCCCCGACACCCTGGTCGGCACCGACTCCCACACCACC 
ATGATCAACGGCCTCGGCGTGCTCGGCTGGGGCGTCGGCGGCATCGAGGCGGAAGCCGCCATGCTCGGCCAG 
CCGGTATCGATGCTGATCCCCGAGGTCATCGGCTTCAAGCTCACCGGCAAGCTGCGCGAGGGCATCACCGCC 
ACCGACCTGGTGCTGACCGTCACGCAGATGCTGCGCAAGAAGGGCGTGGTGGGGAAATTCGTCGAGTTCTAT 
GGCGACGGCCTGGCCGACCTGCCCCTGGCGGACCGCGCCACCATCGCCAACATGGCCCCGGAATACGGCGCC 
ACCTGCGGCTTCTTCCCGGTGGACGAGATCACCCTCGGCTACCTGCGCCTGTCCGGACGCCCGGAAAGCACG 
GTCAAGCTGGTCGAGGCCTACAGCAAGGAACAGGGCCTGTGGCGCGAGAAGGGCCACGAGCCGGTGTTCACC 
GACACCCTGCACCTGGACATGGGCGAAGTCGAGGCCAGCCTGGCCGGACCGAAGCGCCCGCAGGACCGCGTG 
GCCCTGCAGAACGTCGCCAGCGCCTTCAACGAGTTCCTCGGCCTGCAACTGCATCCCTCCAGCACCGAAGAA 
GGCCGCCTGCTCAGCGAGGGCGGCGGCGGCACCGCGGTCGGCGCCAACGCCGCCTTCGGCGAGATCGACTAC 
CAGCACGACGGCCAGACCCACCGCCTGAAGAACGGCGCGGTGGTGATCGCCGCCATCACCTCCTGCACCAAC 
ACCTCCAACCCCAGCGTGATGATGGCCGCCGGGCTGCTGGCGAAGAAGGCGGTGGAGAAAGGCCTGCAACGC 
AAGCCCTGGGTGAAGAGTTCGCTGGCGCCGGGCTCCAAGGTGGTCACCGACTACTTCAAGGCCGCCGGCCTG 
ACCCGCTACCTCGACGAACTCGGCTTCGACCTGGTCGGCTACGGCTGCACCACCTGCATCGGCAACTCCGGG 
CCGCTGCTGGAGCCGATCGAGAAAGCCATCCAGCAGGCCGACCTGACCGTCGCCTCGGTACTCTCCGGCAAC 
CGTAACTTCGAAGGCCGCGTGCACCCGTTGGTGAAGACCAACTGGCTGGCCTCGCCGCCGCTGGTGGTGGCC 
TACGCCCTGGCCGGCAGCGTGCGGATCAACCTCAGCGAGGAACCGCTGGGCACCGGCAAGGACGGCCAGCCG 
GTCTACCTGAAGGACATCTGGCCGAGCCAGAAGGAAATCGCCGAGGCGATCCAGAAGGTCGACACCGAGATG 
TTCCACAAGGAGTACGCCGAGGTCTTCGCCGGCGACGAGAAATGGCAGGCGATCCAGGTGCCGCAGTCGGAC 
ACCTACGAGTGGCAGGCCGACTCCACCTACATCCAGCATCCACCCTTCTTCGAACACATCGCCGAGGCGCCG 
CCGGCCATCGCCGACGTCGAGCAGGCGCGGGTGCTGGCGGTGCTGGGCGACTCGGTGACCACCGACCACATC 
TCCCCTGCCGGCAACATCAAGGCCGACAGCCCGGCCGGCCGCTACCTGCGCGAGCACGGCGTGGAACCGAAG 
GACTTCAACTCCTACGGCTCCCGTCGCGGCAACCATGAGGTGATGATGCGCGGCACCTTCGCCAACATCCGC 
ATCAAGAACGAGATGCTCGGCGGCGAGGAAGGCGGCAATACCCTGTACGTGCCCAGCGGCGAGAAACTGGCG 
ATCTACGACGCGGCCATGCGCTACCAGCAGGACGGCACCCCGCTGGTGATCGTCGCCGGCAAGGAATACGGC 
ACCGGCTCGTCGCGCGACTGGGCGGCCAAGGGTACCAACCTGCTGGGCGTGAAGGCGGTGATCGCGGAGAGC 
TTCGAGCGCATCCACCGCTCCAACCTGGTCGGCATGGGCGTGCTGCCGTTGCAGTTCGAGAACGGCCAGGAC 
CGCAAGAGCCTGAAGCTGACCGGCAAGGAGGTGCTGAACATCCGCGGCCTCGGCGGCGAACTCAAACCGCAC 
ATGCCGCTCAGCGTGGAAGTGACCCGCGAGGACGGCAGCCAGGACAGCTTCAAGGTCCTCTGCCGCATCGAC 
ACCCTCAACGAGGTCGAGTACTTCAAGGCCGGCGGCATCCTCCACTACGTGCTGCGCAGCATGCTCTGA