ViewGene
Locus Name:
GeneID:
Location:

GeneID:1019
TrivialName: PA14_30340
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_30340
PAO1 Ortholog Locus:PA2611
Sequence Length:1398
Protein Length:465
Start: 2628497
Stop: 2629894
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05999.1|AE004689_11 (AE004689) siroheme synthase [Pseudomonas aeruginosa PAO1]
Identities = 463/465 (99%), Positives = 463/465 (99%)
Sequence:
ATGGATTTCCTGCCGCTGTTCCACTCCTTGCAAGGGCGCCTCGCGCTGGTGGTCGGCGGCGGCGAAGTCGCC
CTGCGCAAGGCGCGCCTGCTGGCCGACGCCGGCGCGCGCCTGCGCGTGGTCGCACCGCAGATCCACATCGAA
CTGCGGCATCTGGTGGAGCAGGGCGGCGGCGAGCTGCTCGAACGCGACTACCAGGACGGCGACCAGCCCGGC
TGCGCCCTGATCATCGCCGCCACCGACGACGAACCGCTGAACGCCGAGGTGTCGCGAGCGGCCAATGCGCGC
GGGATACCGGTCAACGTGGTGGACGCGCCGGCCCTGTGCAGCGTGATCTTCCCGGCCATCGTCGACCGTTCG
CCGCTGGTGGTGGCGGTCTCcaGCGGCGGCGATGCGCCCGTGCTGGCGCGGCTGATCCGGGCCAAGCTGGAA
ACCTGGATTCCCTCGACCTACGGGCAACTGGCCGGACTCGCCAGCCGCTTCCGCCACCGGGTCAAGGAATTG
CTACCCGACCTGCAGCAGCGCCGGGTGTTCTGGGAAAACCTGTTCCAGGGCGAGATCGCCGAGCGTGTCCTG
GCCGGCCGTCCGGCGGAAGCCGAGCGTCTCCTGGAGGAGCGCCTGGCCGGCGGCCTCGCGCACATAGCCACG
GGCGAGGTATATCTGGTCGGGGCCGGGCCGGGGGATCCGGACCTGCTGACGTTCCGTGCCCTGCGCCTGATG
CAGCAGGCCGACGTGGTGCTCTACGATCGCCTGGTGGCGCCGTCGATCCTCGAATTATGCCGCCGCGATGCC
GAACGCCTGTACGTCGGCAAGCGCCGCGCCGAACATGCGGTGCCGCAGGATCGGATCAACCGCCTGCTGGTG
GAGCTGGCCAGCCAGGGCAAGCGCGTGCTGCGCCTGAAGGGTGGCGACCCGTTCATCTTCGGCCGCGGCGGT
GAGGAAATCGACGAACTGGCTGCCCGCGGCATACCTTTCCAGGTCGTGCCGGGAATCACCGCGGCCAGCGGC
TGCGCGGCCTACGCCGGGATTCCACTGACTCATCGCGATCATGCGCAATCGGTTCGTTTCGTCACCGGTCAT
CTGAAGGACGGCACCACCGACCTGCCGTGGCAGGACCTGGTAGCGCCTGGCCAGACCCTGGTGTTCTACATG
GGCCTGGTGGGATTGCCGGTGATCTGCGAGCAACTGGTCGCCCACGGTCGCTCCGCACAGACCCCGGCGGCG
CTGATCCAGCAGGGGACCACCGCCCAGCAGCGGGTATTCACCGGAACCCTGGAAAACCTGCCGCAACTGGTG
GCTGAGCACGAAGTGCATGCGCCGACCCTGGTGATCGTCGGCGAAGTCGTGCAACTGCGCGACAAGCTGGCC
TGGTTCGAAGGCGCCCGCGAGGACGCCTGA
Translation:
MDFLPLFHSLQGRLALVVGGGEVALRKARLLADAGARLRVVAPQIHIELRHLVEQGGGELLERDYQDGDQPG
CALIIAATDDEPLNAEVSRAANARGIPVNVVDAPALCSVIFPAIVDRSPLVVAVSSGGDAPVLARLIRAKLE
TWIPSTYGQLAGLASRFRHRVKELLPDLQQRRVFWENLFQGEIAERVLAGRPAEAERLLEERLAGGLAHIAT
GEVYLVGAGPGDPDLLTFRALRLMQQADVVLYDRLVAPSILELCRRDAERLYVGKRRAEHAVPQDRINRLLV
ELASQGKRVLRLKGGDPFIFGRGGEEIDELAARGIPFQVVPGITAASGCAAYAGIPLTHRDHAQSVRFVTGH
LKDGTTDLPWQDLVAPGQTLVFYMGLVGLPVICEQLVAHGRSAQTPAALIQQGTTAQQRVFTGTLENLPQLV
AEHEVHAPTLVIVGEVVQLRDKLAWFEGAREDA*
AnnotationID:1028GeneID:1019
AnnotatorUID: diggins
Modification Date/Time: 2005-04-29 11:11:13
Gene Name:cysG
Confidence Code:2
GeneProduct:siroheme synthase
Cell Localization:()
Synonyms:cobA-2
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:uroporphyrin-III C-methyltransferase
Functional Category Confidence Code:5
COGs:COG0007,COG1648
Secondary Functional Category(ies):
EC Number:2.1.1.107
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO56822.1| (AE016868) siroheme synthase [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 464

 Score =  744 bits (1920), Expect = 0.0
 Identities = 372/462 (80%), Positives = 415/462 (89%), Gaps = 1/462 (0%)

>gb|AAN69593.1|AE016593_1 (AE016788) uroporphyrin-III C-methyltransferase [Pseudomonas putida
           KT2440]
          Length = 463

 Score =  718 bits (1853), Expect = 0.0
 Identities = 360/462 (77%), Positives = 401/462 (86%), Gaps = 1/462 (0%)

>emb|CAD84443.1| (BX321857) cysG_2; Uroporphyrinogen-III methylase [Nitrosomonas
           europaea ATCC 19718]
          Length = 501

 Score =  532 bits (1370), Expect = e-150
 Identities = 278/469 (59%), Positives = 339/469 (72%), Gaps = 11/469 (2%)






 
Structural Features:
COG0007, CysG, Uroporphyrinogen-III methylase [Coenzyme metabolism]
CD-Length = 244 residues,  99.6% aligned
Score =  324 bits (832), Expect = 2e-89
Identities = 151/245 (61%), Positives = 176/245 (71%), Gaps = 2/245 (0%)

COG1648, CysG, Siroheme synthase (precorrin-2 oxidase/ferrochelatase domain) 
[Coenzyme metabolism]
CD-Length = 210 residues, 100.0% aligned
Score =  228 bits (583), Expect = 9e-61
Identities = 112/210 (53%), Positives = 148/210 (70%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2041




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19183




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33175




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1019_cdd.html


    		




 
URL:

















    ReferenceID:34541




  
Author/Investigator(s):
        Spencer JB, Stolowich NJ, Roessner CA, Scott AI.





  
Title:
        The Escherichia coli cysG gene encodes the multifunctional protein, siroheme synthase.




  
PubMed:
        8243665


MedLine:
        




    
Source:
FEBS Lett. 1993 Nov 29;335(1):57-60.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8243665

















    ReferenceID:34542




  
Author/Investigator(s):
        Harborne NR, Griffiths L, Busby SJ, Cole JA.





  
Title:
        Transcriptional control, translation and function of the products of the five open reading frames of the Escherichia coli nir operon.




  
PubMed:
        1435259


MedLine:
        




    
Source:
Mol Microbiol. 1992 Oct;6(19):2805-13.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1435259

















    ReferenceID:34543




  
Author/Investigator(s):
        Koyama M, Katayama S, Kaji M, Taniguchi Y, Matsushita O, Minami J, Morita S, Okabe A.





  
Title:
        A Clostridium perfringens hem gene cluster contains a cysG(B) homologue that is involved in cobalamin biosynthesis.




  
PubMed:
        10585141


MedLine:
        




    
Source:
Microbiol Immunol. 1999;43(10):947-57.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10585141

















    ReferenceID:34544




  
Author/Investigator(s):
        Peakman T, Crouzet J, Mayaux JF, Busby S, Mohan S, Harborne N, Wootton J, Nicolson R, Cole J.





  
Title:
        Nucleotide sequence, organisation and structural analysis of the products of genes in the nirB-cysG region of the Escherichia coli K-12 chromosome.




  
PubMed:
        2200672


MedLine:
        




    
Source:
Eur J Biochem. 1990 Jul 31;191(2):315-23.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2200672









           
Homologs By Global Alignment 

           

       



     
Gene ID:1019
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1835
                           PA2611_tr
                           translation of PA2611
                           465
                           99.56
                           99.56
                           0
                           2353.0
     

     
     
63704
                           gb|AAG05999.1|AE004689_11
                           (AE004689) siroheme synthase [Pseudomonas aeruginosa PAO1]
                           465
                           99.56
                           99.56
                           0
                           2353.0
     

     
     
63705
                           gb|AAO56822.1|
                           (AE016868) siroheme synthase [Pseudomonas syringae pv. tomato str. DC3000]
                           465
                           80.00
                           89.24
                           1
                           1919.0
     

     
     
63706
                           gb|AAN69593.1|AE016593_1
                           (AE016788) uroporphyrin-III C-methyltransferase [Pseudomonas putida KT2440]
                           465
                           77.41
                           86.23
                           2
                           1855.0
     

     
     
63708
                           gb|AAQ58488.1|
                           (AE016912) uroporphyrin-III C-methyltransferase [Chromobacterium violaceum ATCC 12472]
                           471
                           59.02
                           71.12
                           7
                           1337.0
     

     






      

          
GC ORFID: 44021How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2611Glimmer Score: 

          
Start: 2325429Stop: 2324032

          
Length: 1398


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2611 translation of PA2611Homolog Bit Score 910.0

          
Other Homologs:  psyr_15may02_Scaffold17_revised_gene2817 (ORF: BLASTX 739.0), t_PA0510 (ORF: BLASTX 273.0), t_PA1778 (ORF: BLASTX 190.0), psyr_15may02_Scaffold1_revised_gene369 (ORF: BLASTX 184.0), t_PA2611 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGATTTCCTGCCGCTGTTCCACTCCTTGCAAGGGCGCCTCGCGCTGGTGGTCGGCGGCGGCGAAGTCGCC 
CTGCGCAAGGCGCGCCTGCTGGCCGACGCCGGCGCGCGCCTGCGCGTGGTCGCACCGCAGATCCACATCGAA 
CTGCGGCATCTGGTGGAGCAGGGCGGCGGCGAGCTGCTCGAACGCGACTACCAGGACGGCGACCAGCCCGGC 
TGCGCCCTGATCATCGCCGCCACCGACGACGAACCGCTGAACGCCGAGGTGTCGCGAGCGGCCAATGCGCGC 
GGGATACCGGTCAACGTGGTGGACGCGCCGGCCCTGTGCAGCGTGATCTTCCCGGCCATCGTCGACCGTTCG 
CCGCTGGTGGTGGCGGTCTCcaGCGGCGGCGATGCGCCCGTGCTGGCGCGGCTGATCCGGGCCAAGCTGGAA 
ACCTGGATTCCCTCGACCTACGGGCAACTGGCCGGACTCGCCAGCCGCTTCCGCCACCGGGTCAAGGAATTG 
CTACCCGACCTGCAGCAGCGCCGGGTGTTCTGGGAAAACCTGTTCCAGGGCGAGATCGCCGAGCGTGTCCTG 
GCCGGCCGTCCGGCGGAAGCCGAGCGTCTCCTGGAGGAGCGCCTGGCCGGCGGCCTCGCGCACATAGCCACG 
GGCGAGGTATATCTGGTCGGGGCCGGGCCGGGGGATCCGGACCTGCTGACGTTCCGTGCCCTGCGCCTGATG 
CAGCAGGCCGACGTGGTGCTCTACGATCGCCTGGTGGCGCCGTCGATCCTCGAATTATGCCGCCGCGATGCC 
GAACGCCTGTACGTCGGCAAGCGCCGCGCCGAACATGCGGTGCCGCAGGATCGGATCAACCGCCTGCTGGTG 
GAGCTGGCCAGCCAGGGCAAGCGCGTGCTGCGCCTGAAGGGTGGCGACCCGTTCATCTTCGGCCGCGGCGGT 
GAGGAAATCGACGAACTGGCTGCCCGCGGCATACCTTTCCAGGTCGTGCCGGGAATCACCGCGGCCAGCGGC 
TGCGCGGCCTACGCCGGGATTCCACTGACTCATCGCGATCATGCGCAATCGGTTCGTTTCGTCACCGGTCAT 
CTGAAGGACGGCACCACCGACCTGCCGTGGCAGGACCTGGTAGCGCCTGGCCAGACCCTGGTGTTCTACATG 
GGCCTGGTGGGATTGCCGGTGATCTGCGAGCAACTGGTCGCCCACGGTCGCTCCGCACAGACCCCGGCGGCG 
CTGATCCAGCAGGGGACCACCGCCCAGCAGCGGGTATTCACCGGAACCCTGGAAAACCTGCCGCAACTGGTG 
GCTGAGCACGAAGTGCATGCGCCGACCCTGGTGATCGTCGGCGAAGTCGTGCAACTGCGCGACAAGCTGGCC 
TGGTTCGAAGGCGCCCGCGAGGACGCCTGA