ViewGene
Locus Name:
GeneID:
Location:

GeneID:1016
TrivialName: PA14_03880
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_03880
PAO1 Ortholog Locus:PA0298
Sequence Length:1359
Protein Length:452
Start: 349652
Stop: 351010
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03687.1|AE004467_9 (AE004467) probable glutamine synthetase [Pseudomonas aeruginosa PAO1]
Identities = 452/452 (100%), Positives = 452/452 (100%)
Sequence:
ATGACTACCAAGTTAGACCAGCTGACCAGCTGGCTGAAGGAACGCAAGATCACCGAAGTGGAATGCCTGATT
TCCGATCTGACAGGCATCGCCCGCGGCAAGATCTCGCCGACCAACAAATTCATCGCGGAAAAGGGCATGCGC
CTGCCGGAGAGCGTTCTGCTGCAGACCGTCACCGGCGACTACGTGGAAGACGACATTTACTACGACCTGCTC
GACCCGGCCGACATCGATATGGTCTGCCGCCCGGACGAGAATGCCGTGTTCCTCGTCCCCTGGGCCATCGAG
CCCACCGCGATGGTGATCCACGACACCTTCGACAAGCTCGGCAATCCCATCGAGCTGTCGCCGCGCAACATC
CTCAAGCGCGTGTTGAAGATGTATGCCGACAAGGGCTGGCGGCCGATCGTGGCGCCAGAGATGGAGTTCTAC
CTGACCAAGCGCAGCGACGACCCCGACTATCCGCTGCAGGCCCCGGTGGGCCGCTCCGGACGCCAGGAAACC
GGGCGCCAGTCGTTCTCCATCGACGCCGCCAACGAGTTCGACCCGCTGTTCGAGGACATGTACGACTGGTGC
GAGGCCCAGGGCCTTGACCTGGATACGCTGATCCACGAGGAAGGCACCGCGCAGATGGAGATCAACTTCCGT
CATGGCGACGCCCTCGACCTGGCCGACCAGATCCTGGTGTTCAAGCGCACCATGCGCGAGGCCGCGCTCAAG
CACAACGTCGCCGCGACCTTCATGGCCAAGCCGATGACCGGCGAGCCGGGCAGCGCGATGCACCTGCACCAG
AGCATCGTCGACGTGAAGACCGGCAAGAACATCTTCTCCAATGCCGACGGCACCATGAGCGAGCTGTTCCTG
CACCATATCGGCGGCCTGCAGAAGTTCATCCCGGAAGTGCTGCCGCTGTTCGCGCCCAACGTGAACTCGTTC
CGCCGCTTCCTGCCCGATACCTCGGCGCCGGTGAACGTCGAGTGGGGCGAAGAGAACCGTACCGTCGGCCTG
CGCGTGCCGGACTCCAGCCCGGAGAACCGCCGGGTCGAGAACCGTCTCGCCGGCGCCGACGCTAACCCTTAC
CTGGCGCTGGCTGCCAGCCTGCTGTGCGGCTACATCGGCATGGTCGAAGGCATCAAGCCGAGCGCCCAGGTC
AAAGGTCGCGGCTATGAACGCCGCAACCTGCGCCTGCCGCTGACGATCGAAGCGGCGCTGGAGCGCATGGAG
AACTGCAAGCCCCTCGAGCAGTATCTCGGCAGCAAGTTCATCAGCGGCTACGTCGCGGTGAAGCGTGCGGAG
CACGAGAATTTCAAGCGGGTAATCAGCTCCTGGGAGCGGGAATTCCTCCTGCTTTCCGTTTGA
Translation:
MTTKLDQLTSWLKERKITEVECLISDLTGIARGKISPTNKFIAEKGMRLPESVLLQTVTGDYVEDDIYYDLL
DPADIDMVCRPDENAVFLVPWAIEPTAMVIHDTFDKLGNPIELSPRNILKRVLKMYADKGWRPIVAPEMEFY
LTKRSDDPDYPLQAPVGRSGRQETGRQSFSIDAANEFDPLFEDMYDWCEAQGLDLDTLIHEEGTAQMEINFR
HGDALDLADQILVFKRTMREAALKHNVAATFMAKPMTGEPGSAMHLHQSIVDVKTGKNIFSNADGTMSELFL
HHIGGLQKFIPEVLPLFAPNVNSFRRFLPDTSAPVNVEWGEENRTVGLRVPDSSPENRRVENRLAGADANPY
LALAASLLCGYIGMVEGIKPSAQVKGRGYERRNLRLPLTIEAALERMENCKPLEQYLGSKFISGYVAVKRAE
HENFKRVISSWEREFLLLSV*
AnnotationID:1025GeneID:1016
AnnotatorUID: diggins
Modification Date/Time: 2005-03-24 07:07:02
Gene Name:spuB
Confidence Code:1
GeneProduct:glutamine synthetase
Cell Localization:()
Synonyms:glnA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00120
Secondary Functional Category(ies):putative enzyme
EC Number:6.3.1.2
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58735.1| (AE016875) glutamine synthetase [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 452

 Score =  827 bits (2137), Expect = 0.0
 Identities = 397/452 (87%), Positives = 429/452 (94%)

>gb|AAN70748.1|AE016718_7 (AE016793) glutamine synthetase, putative [Pseudomonas putida
           KT2440]
          Length = 452

 Score =  819 bits (2116), Expect = 0.0
 Identities = 397/452 (87%), Positives = 425/452 (94%)

>gb|AAN54336.1|AE015570_10 (AE015570) glutamine synthetase [Shewanella oneidensis MR-1]
          Length = 451

 Score =  684 bits (1764), Expect = 0.0
 Identities = 326/451 (72%), Positives = 384/451 (85%), Gaps = 3/451 (0%)








 
Structural Features:
pfam00120, Gln-synt_C, Glutamine synthetase, catalytic domain. 
CD-Length = 260 residues, 100.0% aligned
Score =  285 bits (731), Expect = 7e-78
Identities = 100/264 (37%), Positives = 139/264 (52%), Gaps = 7/264 (2%)







   
Genomic Context:






   
Comment:


















    ReferenceID:2035




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19177




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33649




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1016_cdd.html


    		




 
URL:

















    ReferenceID:35432




  
Author/Investigator(s):
        Lu CD, Itoh Y, Nakada Y, Jiang Y.





  
Title:
        Functional analysis and regulation of the divergent spuABCDEFGH-spuI operons for polyamine uptake and utilization in Pseudomonas aeruginosa PAO1.




  
PubMed:
        12081945


MedLine:
        




    
Source:
J Bacteriol. 2002 Jul;184(14):3765-73.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12081945









           
Homologs By Global Alignment 

           

       



     
Gene ID:1016
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1828
                           PA0298_tr
                           translation of PA0298
                           452
                           99.99
                           99.99
                           0
                           2358.0
     

     
     
63665
                           gb|AAG03687.1|AE004467_9
                           (AE004467) probable glutamine synthetase [Pseudomonas aeruginosa PAO1]
                           452
                           99.99
                           99.99
                           0
                           2358.0
     

     
     
63666
                           gb|AAO58735.1|
                           (AE016875) glutamine synthetase [Pseudomonas syringae pv. tomato str. DC3000]
                           452
                           87.83
                           94.91
                           0
                           2137.0
     

     
     
63667
                           gb|AAN70748.1|AE016718_7
                           (AE016793) glutamine synthetase, putative [Pseudomonas putida KT2440]
                           452
                           87.83
                           94.02
                           0
                           2116.0
     

     
     
63668
                           gb|AAN68756.1|AE016507_7
                           (AE016785) glutamine synthetase, putative [Pseudomonas putida KT2440]
                           452
                           82.07
                           91.81
                           0
                           2007.0
     

     






      

          
GC ORFID: 44018How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0298Glimmer Score: 

          
Start: 6150994Stop: 6152352

          
Length: 1359


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0298 translation of PA0298Homolog Bit Score 912.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6509 (ORF: BLASTX 827.0), t_PA3356 (ORF: BLASTX 335.0), psyr_15may02_Scaffold8_revised_gene7882 (ORF: BLASTX 332.0), t_PA0298 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACTACCAAGTTAGACCAGCTGACCAGCTGGCTGAAGGAACGCAAGATCACCGAAGTGGAATGCCTGATT 
TCCGATCTGACAGGCATCGCCCGCGGCAAGATCTCGCCGACCAACAAATTCATCGCGGAAAAGGGCATGCGC 
CTGCCGGAGAGCGTTCTGCTGCAGACCGTCACCGGCGACTACGTGGAAGACGACATTTACTACGACCTGCTC 
GACCCGGCCGACATCGATATGGTCTGCCGCCCGGACGAGAATGCCGTGTTCCTCGTCCCCTGGGCCATCGAG 
CCCACCGCGATGGTGATCCACGACACCTTCGACAAGCTCGGCAATCCCATCGAGCTGTCGCCGCGCAACATC 
CTCAAGCGCGTGTTGAAGATGTATGCCGACAAGGGCTGGCGGCCGATCGTGGCGCCAGAGATGGAGTTCTAC 
CTGACCAAGCGCAGCGACGACCCCGACTATCCGCTGCAGGCCCCGGTGGGCCGCTCCGGACGCCAGGAAACC 
GGGCGCCAGTCGTTCTCCATCGACGCCGCCAACGAGTTCGACCCGCTGTTCGAGGACATGTACGACTGGTGC 
GAGGCCCAGGGCCTTGACCTGGATACGCTGATCCACGAGGAAGGCACCGCGCAGATGGAGATCAACTTCCGT 
CATGGCGACGCCCTCGACCTGGCCGACCAGATCCTGGTGTTCAAGCGCACCATGCGCGAGGCCGCGCTCAAG 
CACAACGTCGCCGCGACCTTCATGGCCAAGCCGATGACCGGCGAGCCGGGCAGCGCGATGCACCTGCACCAG 
AGCATCGTCGACGTGAAGACCGGCAAGAACATCTTCTCCAATGCCGACGGCACCATGAGCGAGCTGTTCCTG 
CACCATATCGGCGGCCTGCAGAAGTTCATCCCGGAAGTGCTGCCGCTGTTCGCGCCCAACGTGAACTCGTTC 
CGCCGCTTCCTGCCCGATACCTCGGCGCCGGTGAACGTCGAGTGGGGCGAAGAGAACCGTACCGTCGGCCTG 
CGCGTGCCGGACTCCAGCCCGGAGAACCGCCGGGTCGAGAACCGTCTCGCCGGCGCCGACGCTAACCCTTAC 
CTGGCGCTGGCTGCCAGCCTGCTGTGCGGCTACATCGGCATGGTCGAAGGCATCAAGCCGAGCGCCCAGGTC 
AAAGGTCGCGGCTATGAACGCCGCAACCTGCGCCTGCCGCTGACGATCGAAGCGGCGCTGGAGCGCATGGAG 
AACTGCAAGCCCCTCGAGCAGTATCTCGGCAGCAAGTTCATCAGCGGCTACGTCGCGGTGAAGCGTGCGGAG 
CACGAGAATTTCAAGCGGGTAATCAGCTCCTGGGAGCGGGAATTCCTCCTGCTTTCCGTTTGA