ViewGene
Locus Name:
GeneID:
Location:

GeneID:1008
TrivialName: PA14_63850
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_63850
PAO1 Ortholog Locus:PA4829
Sequence Length:1404
Protein Length:467
Start: 5692366
Stop: 5690963
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08214.1|AE004896_4 (AE004896) dihydrolipoamide dehydrogenase 3 [Pseudomonas aeruginosa PAO1]
Identities = 466/467 (99%), Positives = 466/467 (99%)
Sequence:
ATGATGGAAAGCTATGACGTGATCGTGATCGGCGCCGGTCCGGGGGGCTACAACGCTGCCATTCGCGCCGGC
CAGCTAGGCCTGAAGGTGGCCTGCGTGGAGGGTCGCGAAACCCTCGGCGGGACTTGCCTGAACGTCGGCTGT
ATGCCGTCCAAGGCCCTGTTGCACGCTTCGGAGCTATACGCCGCGGCGAGCGGCGGCGAGTTCGCCCGGCTC
GGCATCCGGGTCAGCCCGGAATTGGACCTGGCGCAGATGATGAAGCAGAAGGACGAGAGCGTCGCCGCCCTG
ACCCGTGGCGTCGAATTCCTTTTCCGCAAGCACAAAGTGCAGTGGATCAAGGGCTGGGCGCGCTTGCAAGGC
GAGGGCCGGGTCGGCGTGGCCCTTGCCGACGGCGGCCACGCGCAACTGGAGGCGCGCGACATCGTCATCGCC
ACCGGTTCGGAACCGGCGCCGCTGCCCGGCGTGCCGGTGGACAACCAGCGCATCCTCGACTCCACCGGCGCC
CTGGAGTTGGCCGAAGTGCCTCGCCACCTGGTGGTGATCGGCGCCGGCGTGATCGGCCTGGAGCTTGGTTCG
GTCTGGCGCCGGCTGGGCGCACAGGTCACGGTGCTGGAGTACCTCGAGCGGATCTGCCCGGGGCTGGATGGG
GAAACCGCAAGGACCCTCCAGCGCGCCCTGACCCGCCAGGGCATGCGGTTTCGCCTCGGCACCCGCGTGGTC
GCCGCGCGCAGTGGCGAGCAGGGTGTCGAACTCGACCTGCAACCGGCCGCCGGCGGCGCTACGGAATCGCTG
CAGGCGGACTACGTGCTGGTGGCCATCGGCCGGCGCCCCTATACCGAAGGACTGGGGCTGGAGACGGTCGGC
CTGGCCAGCGATCGTCGCGGCATGCTGGAGAACCAGGGACAGCGCAGTGCGGCGCCGGGCGTCTGGGTGATC
GGCGACGTGACCTCGGGACCGATGCTGGCGCACAAGGCCGAGGAAGAGGCGATCGTCTGCATCGAGCGGATC
GCCGGGCATGCGGCGGAGATGAACGCCGAGGTGATTCCGTCGGTCATCTACACCCAGCCGGAAGTGGCCAGC
GTCGGCCTCGGCGAGGAGCAGCTGCAGGCGGCGAGGCGCGAGTACAAGGTCGGACGCTTCCCGTTCAGCGCC
AACAGCCGGGCGAAGATCAACCACGAGAGCGAAGGCTTCATCAAGATCCTCTCCGATGCCCGCAGCGACCAG
GTGCTCGGCGTGCACATGATCGGGCCGGGCGTCAGCGAGATGATCGGCGAAGCCTGCGTGGCCATGGAGTTC
AGCGCTTCGGCGGAGGACCTCGCGCTGACCTGCCATCCGCATCCGACCCGCTCCGAGGCGCTGCGCCAGGCG
GCGATGGACGTGCATGGCCGGGCCATGCAGAACTGA
Translation:
MMESYDVIVIGAGPGGYNAAIRAGQLGLKVACVEGRETLGGTCLNVGCMPSKALLHASELYAAASGGEFARL
GIRVSPELDLAQMMKQKDESVAALTRGVEFLFRKHKVQWIKGWARLQGEGRVGVALADGGHAQLEARDIVIA
TGSEPAPLPGVPVDNQRILDSTGALELAEVPRHLVVIGAGVIGLELGSVWRRLGAQVTVLEYLERICPGLDG
ETARTLQRALTRQGMRFRLGTRVVAARSGEQGVELDLQPAAGGATESLQADYVLVAIGRRPYTEGLGLETVG
LASDRRGMLENQGQRSAAPGVWVIGDVTSGPMLAHKAEEEAIVCIERIAGHAAEMNAEVIPSVIYTQPEVAS
VGLGEEQLQAARREYKVGRFPFSANSRAKINHESEGFIKILSDARSDQVLGVHMIGPGVSEMIGEACVAMEF
SASAEDLALTCHPHPTRSEALRQAAMDVHGRAMQN*
AnnotationID:1017GeneID:1008
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 06:06:10
Gene Name:lpd3
Confidence Code:2
GeneProduct:dihydrolipoamide dehydrogenase 3
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1249,pfam00070,pfam02852
Secondary Functional Category(ies):
EC Number:1.8.1.4
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70931.1|AE016737_2 (AE016794) dihydrolipoamide dehydrogenase 3 [Pseudomonas putida
           KT2440]
          Length = 466

 Score =  714 bits (1844), Expect = 0.0
 Identities = 349/465 (75%), Positives = 402/465 (86%)

>gb|AAK22329.1| (AE005707) 2-oxoglutarate dehydrogenase, E3 component, lipoamide
           dehydrogenase [Caulobacter crescentus CB15]
          Length = 475

 Score =  643 bits (1658), Expect = 0.0
 Identities = 311/465 (66%), Positives = 377/465 (81%)

>gb|AAN03817.1|AF497852_3 (AF497852) dihydrolipoamide dehydrogenase [Methylobacterium
           extorquens]
          Length = 467

 Score =  510 bits (1313), Expect = e-144
 Identities = 266/465 (57%), Positives = 341/465 (73%), Gaps = 4/465 (0%)






 
Structural Features:
COG1249, Lpd, Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase 
(E3) component, and related enzymes 
[Energy production and conversion]
CD-Length = 454 residues, 100.0% aligned
Score =  512 bits (1319), Expect = 4e-146
Identities = 220/459 (47%), Positives = 299/459 (65%), Gaps = 7/459 (1%)

pfam00070, Pyr_redox, Pyridine nucleotide-disulphide oxidoreductase.
CD-Length = 288 residues, 100.0% aligned
Score =  183 bits (466), Expect = 4e-47
Identities = 122/326 (37%), Positives = 159/326 (48%), Gaps = 45/326 (13%)

pfam02852, Pyr_redox_dim, Pyridine nucleotide-disulphide oxidoreductase, 
dimerisation domain.
CD-Length = 110 residues, 100.0% aligned
Score =  142 bits (359), Expect = 9e-35
Identities = 56/110 (50%), Positives = 80/110 (72%)






   
Genomic Context:






   
Comment:


















    ReferenceID:2019




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19161




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35220




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1008_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:1008
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1814
                           PA4829_tr
                           translation of PA4829
                           467
                           99.78
                           99.78
                           0
                           2364.0
     

     
     
63019
                           gb|AAG08214.1|AE004896_4
                           (AE004896) dihydrolipoamide dehydrogenase 3 [Pseudomonas aeruginosa PAO1]
                           467
                           99.78
                           99.78
                           0
                           2364.0
     

     
     
63020
                           gb|AAN70931.1|AE016737_2
                           (AE016794) dihydrolipoamide dehydrogenase 3 [Pseudomonas putida KT2440]
                           467
                           74.73
                           86.08
                           1
                           1841.0
     

     
     
63021
                           emb|CAA39235.1|
                           (X55704) dihydrolipoamide dehydrogenase [Pseudomonas putida]
                           467
                           73.87
                           85.22
                           1
                           1814.0
     

     
     
63022
                           gb|AAK22329.1|
                           (AE005707) 2-oxoglutarate dehydrogenase, E3 component, lipoamide dehydrogenase [Caulobacter crescentus CB15]
                           475
                           65.47
                           79.36
                           8
                           1654.0
     

     






      

          
GC ORFID: 44010How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4829Glimmer Score: 

          
Start: 4956046Stop: 4954643

          
Length: 1404


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4829 translation of PA4829Homolog Bit Score 915.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene999 (ORF: BLASTX 403.0), t_PA1587 (ORF: BLASTX 402.0), t_PA2250 (ORF: BLASTX 328.0), t_PA4829 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATGGAAAGCTATGACGTGATCGTGATCGGCGCCGGTCCGGGGGGCTACAACGCTGCCATTCGCGCCGGC 
CAGCTAGGCCTGAAGGTGGCCTGCGTGGAGGGTCGCGAAACCCTCGGCGGGACTTGCCTGAACGTCGGCTGT 
ATGCCGTCCAAGGCCCTGTTGCACGCTTCGGAGCTATACGCCGCGGCGAGCGGCGGCGAGTTCGCCCGGCTC 
GGCATCCGGGTCAGCCCGGAATTGGACCTGGCGCAGATGATGAAGCAGAAGGACGAGAGCGTCGCCGCCCTG 
ACCCGTGGCGTCGAATTCCTTTTCCGCAAGCACAAAGTGCAGTGGATCAAGGGCTGGGCGCGCTTGCAAGGC 
GAGGGCCGGGTCGGCGTGGCCCTTGCCGACGGCGGCCACGCGCAACTGGAGGCGCGCGACATCGTCATCGCC 
ACCGGTTCGGAACCGGCGCCGCTGCCCGGCGTGCCGGTGGACAACCAGCGCATCCTCGACTCCACCGGCGCC 
CTGGAGTTGGCCGAAGTGCCTCGCCACCTGGTGGTGATCGGCGCCGGCGTGATCGGCCTGGAGCTTGGTTCG 
GTCTGGCGCCGGCTGGGCGCACAGGTCACGGTGCTGGAGTACCTCGAGCGGATCTGCCCGGGGCTGGATGGG 
GAAACCGCAAGGACCCTCCAGCGCGCCCTGACCCGCCAGGGCATGCGGTTTCGCCTCGGCACCCGCGTGGTC 
GCCGCGCGCAGTGGCGAGCAGGGTGTCGAACTCGACCTGCAACCGGCCGCCGGCGGCGCTACGGAATCGCTG 
CAGGCGGACTACGTGCTGGTGGCCATCGGCCGGCGCCCCTATACCGAAGGACTGGGGCTGGAGACGGTCGGC 
CTGGCCAGCGATCGTCGCGGCATGCTGGAGAACCAGGGACAGCGCAGTGCGGCGCCGGGCGTCTGGGTGATC 
GGCGACGTGACCTCGGGACCGATGCTGGCGCACAAGGCCGAGGAAGAGGCGATCGTCTGCATCGAGCGGATC 
GCCGGGCATGCGGCGGAGATGAACGCCGAGGTGATTCCGTCGGTCATCTACACCCAGCCGGAAGTGGCCAGC 
GTCGGCCTCGGCGAGGAGCAGCTGCAGGCGGCGAGGCGCGAGTACAAGGTCGGACGCTTCCCGTTCAGCGCC 
AACAGCCGGGCGAAGATCAACCACGAGAGCGAAGGCTTCATCAAGATCCTCTCCGATGCCCGCAGCGACCAG 
GTGCTCGGCGTGCACATGATCGGGCCGGGCGTCAGCGAGATGATCGGCGAAGCCTGCGTGGCCATGGAGTTC 
AGCGCTTCGGCGGAGGACCTCGCGCTGACCTGCCATCCGCATCCGACCCGCTCCGAGGCGCTGCGCCAGGCG 
GCGATGGACGTGCATGGCCGGGCCATGCAGAACTGA