ViewGene
Locus Name:
GeneID:
Location:

GeneID:1002
TrivialName: PA14_02470
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_02470
PAO1 Ortholog Locus:PA0196
Sequence Length:1437
Protein Length:478
Start: 222339
Stop: 223775
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03585.1|AE004457_6 (AE004457) pyridine nucleotide transhydrogenase, beta subunit [Pseudomonas aeruginosa PAO1]
Identities = 478/478 (100%), Positives = 478/478 (100%)
Sequence:
ATGAGCATGAACCTGGTCACCCTCCTGTACCTCATCGCCTCGGTCTGCTTCATCCAGGCCCTGAAAGGCCTG
TCGCACCCGACCACCTCGCGCCGCGGCAACCTCTTCGGCATGGTCGGCATGGCCATCGCCGTGGCCACCACC
GTCGGCCTGGTGTTCAAGCTGGGCGCGGAGATCGCCACCACCGGCGTCGGCTACATCGTCGTCGGCCTGCTG
GTCGGCGGCACCGCCGGCTCGATCATGGCCAAGCGCGTCGAGATGACCAAGATGCCCGAGCTGGTCGCCTTC
ATGCACAGCATGATCGGCCTGGCCGCGGTGTTCATCGCCATCGCCGCGGTGGTCGAGCCGCAGTCGCTGGGG
ATCGTCGCGCACCTGGGCGACACCATTCCCACCGGCAACCGCCTGGAGCTGTTCCTCGGCGCGGCCATCGGC
GCCATCACCTTCTCCGGTTCGGTGATCGCCTTCGGCAAGCTCTCCGGCAAGTACAAGTTCCGCCTGTTCCAG
GGCACCCCGGTGCAGTTCTCCGGGCAGCACCTGCTGAACCTGGTGCTCGGCCTGGCGACCCTCGGCCTGGGC
CTGGTGTTCATGTTCACCGGCAACCTCACCGCCTTCGCCGTGATGCTGGCGCTGGCCTTCGTCCTCGGCGTG
CTGATCATCATCCCCATCGGCGGCGCCGACATGCCGGTGGTGGTGTCGATGCTCAACTCCTATTCCGGCTGG
GCCGCGGCGGGGATCGGCTTCTCGCTGAACAACTCGATGCTGATCATCGCCGGCTCGCTGGTCGGCTCCTCG
GGTGCGATCCTCTCCTACATCATGTGCAAGGCGATGAACCGCTCGTTCTTCAACGTCATCCTCGGCGGCTTC
GGCGCCGAAGCCGACGCCGGCGGCCCGGCGGGCTCCAAGGAGCAGCGTCCGGTGAAGTCCGGTTCGGCCGAC
GATGCCTCGTTCCTGCTGACCAACGCCGACAGCGTGATCATCGTCCCCGGCTACGGCCTGGCGGTGGCCCGC
GCCCAGCACGCGCTGATGGAACTGGCGGAGAAGCTGACCCATCGCGGGGTCACCGTGAAGTTCGCCATCCAC
CCGGTCGCCGGGCGCATGCCGGGGCACATGAACGTGCTGCTGGCCGAGGCCGAGGTGCCCTACGAGCAGGTG
TTCGAGATGGAGGACATCAACTCCGAGTTCGGCCAGACCGACGTGGTCCTGGTGCTCGGTGCCAACGACGTG
GTCAACCCGGCGGCGAAGAACGATCCGAAGTCGCCCATCGCCGGCATGCCGATCCTCGAGGCCTACAAGGCC
AAGACCGTGATCGTCAACAAGCGCTCCATGGCCAGCGGCTACGCCGGCCTGGACAACGAGCTGTTCTACCTG
GACAAGACCATGATGGTGTTCGGCGACGCCAAGAAGGTCATCGAAGACATGGTCAAGGCGGTGGAGTGA
Translation:
MSMNLVTLLYLIASVCFIQALKGLSHPTTSRRGNLFGMVGMAIAVATTVGLVFKLGAEIATTGVGYIVVGLL
VGGTAGSIMAKRVEMTKMPELVAFMHSMIGLAAVFIAIAAVVEPQSLGIVAHLGDTIPTGNRLELFLGAAIG
AITFSGSVIAFGKLSGKYKFRLFQGTPVQFSGQHLLNLVLGLATLGLGLVFMFTGNLTAFAVMLALAFVLGV
LIIIPIGGADMPVVVSMLNSYSGWAAAGIGFSLNNSMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVILGGF
GAEADAGGPAGSKEQRPVKSGSADDASFLLTNADSVIIVPGYGLAVARAQHALMELAEKLTHRGVTVKFAIH
PVAGRMPGHMNVLLAEAEVPYEQVFEMEDINSEFGQTDVVLVLGANDVVNPAAKNDPKSPIAGMPILEAYKA
KTVIVNKRSMASGYAGLDNELFYLDKTMMVFGDAKKVIEDMVKAVE*
AnnotationID:1011GeneID:1002
AnnotatorUID: diggins
Modification Date/Time: 2005-03-23 11:11:59
Gene Name:pntB
Confidence Code:2
GeneProduct:pyridine nucleotide transhydrogenase, beta subunit
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1282,pfam02233
Secondary Functional Category(ies):
EC Number:1.6.1.1
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58883.1| (AE016875) NAD(P) transhydrogenase, beta subunit [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 484

 Score =  840 bits (2171), Expect = 0.0
 Identities = 432/482 (89%), Positives = 453/482 (93%), Gaps = 4/482 (0%)

>gb|AAN65789.1|AE016205_2 (AE016774) pyridine nucleotide transhydrogenase, beta subunit
           [Pseudomonas putida KT2440]
          Length = 478

 Score =  840 bits (2171), Expect = 0.0
 Identities = 423/478 (88%), Positives = 454/478 (94%)

>emb|CAE37792.1| (BX640430) NAD(P) transhydrogenase subunit beta [Bordetella
           parapertussis]
          Length = 478

 Score =  743 bits (1918), Expect = 0.0
 Identities = 383/478 (80%), Positives = 416/478 (87%), Gaps = 1/478 (0%)








 
Structural Features:
COG1282, PntB, NAD/NADP transhydrogenase beta subunit 
[Energy production and conversion]
CD-Length = 463 residues, 100.0% aligned
Score =  645 bits (1666), Expect = 0.0
Identities = 350/477 (73%), Positives = 401/477 (84%), Gaps = 14/477 (2%)

pfam02233, PNTB, NAD(P) transhydrogenase beta subunit. 
CD-Length = 464 residues, 100.0% aligned
Score =  540 bits (1392), Expect = 2e-154
Identities = 267/478 (55%), Positives = 333/478 (69%), Gaps = 18/478 (3%)







   
Genomic Context:






   
Comment:


















    ReferenceID:2007




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:19149




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33564




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1002_cdd.html


    		




 
URL:

















    ReferenceID:35478




  
Author/Investigator(s):
        Clarke DM, Loo TW, Gillam S, Bragg PD.





  
Title:
        Nucleotide sequence of the pntA and pntB genes encoding the pyridine nucleotide transhydrogenase of Escherichia coli.




  
PubMed:
        3525165


MedLine:
        




    
Source:
Eur J Biochem. 1986 Aug 1;158(3):647-53.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3525165

















    ReferenceID:35479




  
Author/Investigator(s):
        Williams R, Cotton NP, Thomas CM, Jackson JB.





  
Title:
        Cloning and sequencing of the genes for the proton-translocating nicotinamide nucleotide transhydrogenase from Rhodospirillum rubrum and the implications for the domain structure of the enzyme.




  
PubMed:
        8075801


MedLine:
        




    
Source:
Microbiology. 1994 Jul;140 ( Pt 7):1595-604.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8075801









           
Homologs By Global Alignment 

           

       



     
Gene ID:1002
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1802
                           PA0196_tr
                           translation of PA0196
                           478
                           99.99
                           99.99
                           0
                           2371.0
     

     
     
62881
                           gb|AAG03585.1|AE004457_6
                           (AE004457) pyridine nucleotide transhydrogenase, beta subunit [Pseudomonas aeruginosa PAO1]
                           478
                           99.99
                           99.99
                           0
                           2371.0
     

     
     
62882
                           gb|AAO58883.1|
                           (AE016875) NAD(P) transhydrogenase, beta subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           484
                           89.25
                           93.59
                           6
                           2174.0
     

     
     
62883
                           gb|AAN65789.1|AE016205_2
                           (AE016774) pyridine nucleotide transhydrogenase, beta subunit [Pseudomonas putida KT2440]
                           478
                           88.49
                           94.97
                           0
                           2171.0
     

     
     
62884
                           gb|AAN62246.1|AF440524_33
                           (AF440524) putative pyridine nucleotide transhydrogenase, beta subunit [Pseudomonas aeruginosa]
                           487
                           87.67
                           94.04
                           9
                           2149.0
     

     






      

          
GC ORFID: 44004How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0196Glimmer Score: 

          
Start: 6023680Stop: 6025116

          
Length: 1437


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0196 translation of PA0196Homolog Bit Score 917.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene2885 (ORF: BLASTX 838.0), t_PA0196 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCATGAACCTGGTCACCCTCCTGTACCTCATCGCCTCGGTCTGCTTCATCCAGGCCCTGAAAGGCCTG 
TCGCACCCGACCACCTCGCGCCGCGGCAACCTCTTCGGCATGGTCGGCATGGCCATCGCCGTGGCCACCACC 
GTCGGCCTGGTGTTCAAGCTGGGCGCGGAGATCGCCACCACCGGCGTCGGCTACATCGTCGTCGGCCTGCTG 
GTCGGCGGCACCGCCGGCTCGATCATGGCCAAGCGCGTCGAGATGACCAAGATGCCCGAGCTGGTCGCCTTC 
ATGCACAGCATGATCGGCCTGGCCGCGGTGTTCATCGCCATCGCCGCGGTGGTCGAGCCGCAGTCGCTGGGG 
ATCGTCGCGCACCTGGGCGACACCATTCCCACCGGCAACCGCCTGGAGCTGTTCCTCGGCGCGGCCATCGGC 
GCCATCACCTTCTCCGGTTCGGTGATCGCCTTCGGCAAGCTCTCCGGCAAGTACAAGTTCCGCCTGTTCCAG 
GGCACCCCGGTGCAGTTCTCCGGGCAGCACCTGCTGAACCTGGTGCTCGGCCTGGCGACCCTCGGCCTGGGC 
CTGGTGTTCATGTTCACCGGCAACCTCACCGCCTTCGCCGTGATGCTGGCGCTGGCCTTCGTCCTCGGCGTG 
CTGATCATCATCCCCATCGGCGGCGCCGACATGCCGGTGGTGGTGTCGATGCTCAACTCCTATTCCGGCTGG 
GCCGCGGCGGGGATCGGCTTCTCGCTGAACAACTCGATGCTGATCATCGCCGGCTCGCTGGTCGGCTCCTCG 
GGTGCGATCCTCTCCTACATCATGTGCAAGGCGATGAACCGCTCGTTCTTCAACGTCATCCTCGGCGGCTTC 
GGCGCCGAAGCCGACGCCGGCGGCCCGGCGGGCTCCAAGGAGCAGCGTCCGGTGAAGTCCGGTTCGGCCGAC 
GATGCCTCGTTCCTGCTGACCAACGCCGACAGCGTGATCATCGTCCCCGGCTACGGCCTGGCGGTGGCCCGC 
GCCCAGCACGCGCTGATGGAACTGGCGGAGAAGCTGACCCATCGCGGGGTCACCGTGAAGTTCGCCATCCAC 
CCGGTCGCCGGGCGCATGCCGGGGCACATGAACGTGCTGCTGGCCGAGGCCGAGGTGCCCTACGAGCAGGTG 
TTCGAGATGGAGGACATCAACTCCGAGTTCGGCCAGACCGACGTGGTCCTGGTGCTCGGTGCCAACGACGTG 
GTCAACCCGGCGGCGAAGAACGATCCGAAGTCGCCCATCGCCGGCATGCCGATCCTCGAGGCCTACAAGGCC 
AAGACCGTGATCGTCAACAAGCGCTCCATGGCCAGCGGCTACGCCGGCCTGGACAACGAGCTGTTCTACCTG 
GACAAGACCATGATGGTGTTCGGCGACGCCAAGAAGGTCATCGAAGACATGGTCAAGGCGGTGGAGTGA