Score E
Sequences producing significant alignments: (bits) Value
gnl|CDD|12171 COG2818, Tag, 3-methyladenine DNA glycosylase... 271 6e-74
gnl|CDD|15536 pfam03352, Adenine_glyco, Methyladenine glyco... 266 2e-72
gnl|CDD|25600 pfam00730, HhH-GPD, HhH-GPD superfamily base ... 27 2.4
gnl|CDD|10513 COG0643, CheA, Chemotaxis protein histidine k... 26 4.6
>gnl|CDD|12171 COG2818, Tag, 3-methyladenine DNA glycosylase [DNA replication,
recombination, and repair]
Length = 188
Score = 271 bits (695), Expect = 6e-74
Identities = 105/186 (56%), Positives = 128/186 (68%), Gaps = 5/186 (2%)
Query: 1 MPRCFWC-NDDPLYMAYHDEEWGVPQRDPDALFELLLLEGFQAGLSWITVLKKRERYREV 59
Sbjct: 1 MNRCAWAGGLDPLYLAYHDTEWGVPLHDDQRLFELLCLEGFQAGLSWLTVLKKREAFREA 60
Query: 60 LFGFDVQRVAQMSDAEIDELMLDPGIIRNRAKLNAARQNAQAWLELDDPAG----FLWSF 115
Sbjct: 61 FHGFDPEKVAAMTEEDVERLLADAGIIRNRGKIKATINNARAVLELQKEFGSFSEFLWSF 120
Query: 116 VGGQPKINHFAGRAEVPAITPEAEAMSKALRKAGFNFVGPTICYAFMQASGMVMDHTQDC 175
Sbjct: 121 VGGKPSRNQVNDGSEVPASTELSDAMSKALKKRGFKFVGPTTVYAFMQATGLVNDHAEGC 180
Query: 176 DRYAQL 181
Sbjct: 181 PCRREI 186
>gnl|CDD|15536 pfam03352, Adenine_glyco, Methyladenine glycosylase. The
DNA-3-methyladenine glycosylase I is constitutively
expressed and is specific for the alkylated
3-methyladenine DNA
Length = 180
Score = 266 bits (681), Expect = 2e-72
Identities = 110/180 (61%), Positives = 135/180 (75%), Gaps = 5/180 (2%)
Query: 6 WCN-DDPLYMAYHDEEWGVPQRDPDALFELLLLEGFQAGLSWITVLKKRERYREVLFGFD 64
Sbjct: 1 WCTPKDPLYIAYHDEEWGVPLHDDKKLFELLCLEGFQAGLSWITILKKRERYREAFYGFD 60
Query: 65 VQRVAQMSDAEIDELMLDPGIIRNRAKLNAARQNAQAWLEL----DDPAGFLWSFVGGQP 120
Sbjct: 61 PEKIARMTDKDIDRLMTDPGIIRNRAKIEAIVGNARAYLKIEQSYGSFSKFLWSFVNHKP 120
Query: 121 KINHFAGRAEVPAITPEAEAMSKALRKAGFNFVGPTICYAFMQASGMVMDHTQDCDRYAQ 180
Sbjct: 121 TQSQFVDLSEVPTKTPEAKAISKDLKRRGFRFVGPTTCYAFMQAAGLVNDHLQDCFRYPE 180
>gnl|CDD|25600 pfam00730, HhH-GPD, HhH-GPD superfamily base excision DNA repair
protein. This family contains a diverse range of
structurally related DNA repair proteins. The superfamily
is called the HhH-GPD family after its hallmark
Helix-hairpin-helix and Gly/Pro rich loop followed by a
conserved aspartate. This includes endonuclease III,
EC:4.2.99.18 and MutY an A/G-specific adenine
glycosylase, both have a C terminal 4Fe-4S cluster. The
family also includes 8-oxoguanine DNA glycosylases. The
methyl-CPG binding protein MBD4 also contains a related
domain, that is a thymine DNA glycosylase. The family
also includes DNA-3-methyladenine glycosylase II
EC:3.2.2.21 and other members of the AlkA family
Length = 145
Score = 27.3 bits (60), Expect = 2.4
Identities = 13/48 (27%), Positives = 19/48 (39%)
Query: 44 LSWITVLKKRERYREVLFGFDVQRVAQMSDAEIDELMLDPGIIRNRAK 91
Sbjct: 9 TSDKAANKIYERLFERYGFPTPEALAAADEEELRELIKGLGFYRRKAK 56
>gnl|CDD|10513 COG0643, CheA, Chemotaxis protein histidine kinase and related
kinases [Cell motility and secretion / Signal
transduction mechanisms]
Length = 716
Score = 26.4 bits (58), Expect = 4.6
Identities = 7/17 (41%), Positives = 9/17 (52%)
Query: 68 VAQMSDAEIDELMLDPG 84
Sbjct: 508 AETLSDEEILNLIFAPG 524
Lambda K H
0.324 0.139 0.446
Gapped
Lambda K H
0.267 0.0533 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 1100, Extension: 100
Number of Hits to DB: 3,328,825
Number of Sequences: 0
Number of extensions: 316590
Number of successful extensions: 152
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 149
length of query: 0
length of database: 5,506,404
effective HSP length: 88
effective length of query: -88
effective length of database: 3,918,972
effective search space: -344869536
effective search space used: 372302340
T: 11
A: 40
X1: 1500 (701.0 bits)
X2: 3800 (1463.8 bits)
X3: 6400 (2465.3 bits)
S1: 4000 (1872.2 bits)
S2: 57 (26.2 bits)