RPS-BLAST 2.2.6 [Apr-09-2003]

Database: All 
           18,039 sequences; 5,506,404 total letters



Query= 3958 (200 letters)

Distribution of 16 Blast Hits on the Query Sequence




                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gnl|CDD|27218  pfam07111, HCR, Alpha helical coiled-coil rod...    30   0.34  
gnl|CDD|22637  smart00709, Zpr1, Duplicated domain in the ep...    29   1.1   
gnl|CDD|21335  KOG3552, KOG3552, FERM domain protein FRM-8 [...    29   1.1   
gnl|CDD|18381  KOG0586, KOG0586, Serine/threonine protein ki...    28   1.5   
gnl|CDD|19990  KOG2204, KOG2204, Mannosyl-oligosaccharide al...    28   1.8   
gnl|CDD|7039  pfam03367, zf-ZPR1, ZPR1 zinc-finger domain. T...    27   2.2   
gnl|CDD|18046  KOG0250, KOG0250, DNA repair protein RAD18 (S...    28   2.4   
gnl|CDD|21748  KOG3970, KOG3970, Predicted E3 ubiquitin liga...    27   4.8   
gnl|CDD|18775  KOG0982, KOG0982, Centrosomal protein Nuf [Ce...    27   4.8   
gnl|CDD|16998  pfam01576, Myosin_tail_1, Myosin tail. The my...    26   5.6   
gnl|CDD|27569  pfam07462, MSP1_C, Merozoite surface protein ...    26   5.7   
gnl|CDD|2058  pfam01519, DUF16, Protein of unknown function ...    26   5.7   
gnl|CDD|21435  KOG3655, KOG3655, Drebrins and related actin ...    26   6.6   
gnl|CDD|26467  pfam06046, Sec6, Exocyst complex component Se...    26   7.9   
gnl|CDD|22417  KOG4643, KOG4643, Uncharacterized coiled-coil...    25   9.8   

>gnl|CDD|27218 pfam07111, HCR, Alpha helical coiled-coil rod protein (HCR). This family consists of several mammalian alpha helical coiled-coil rod HCR proteins. The function of HCR is unknown but it has been implicated in psoriasis in humans and is thought to affect keratinocyte proliferation Length = 760 Score = 30.2 bits (67), Expect = 0.34 Identities = 39/136 (28%), Positives = 59/136 (43%), Gaps = 12/136 (8%)
Query:  41   RTVAALPAVDDRPPPLAPVRSQAVAPAYEAEPPAAELDREQAIQFMQMLRDEGDPRSPPL  100
Sbjct:  443  RKVHTIKGLIARKLALAQLRQESCPPSEAAPPLDADLSLE-----LEQLREE---RNRLD  494

Query:  101  GGLQPRQGATAQELADPKQYQAFEERQTRELVQAYTSGVQQIPEIRA----RIEAAEQGG  156
Sbjct:  495  AELQLSAHLIQQEVGRAREQGEAERQQLIEVAQQLEQELQQAQESLASLGLQLEAARQGQ  554

Query:  157  ERSAEEIDEARAALGQ  172
Sbjct:  555  QESTEEAASLRQELTQ  570


>gnl|CDD|22637 smart00709, Zpr1, Duplicated domain in the epidermal growth factor- and elongation factor-1alpha-binding protein Zpr1. Also present in archaeal proteins Length = 160 Score = 28.6 bits (64), Expect = 1.1 Identities = 21/96 (21%), Positives = 38/96 (39%), Gaps = 17/96 (17%)
Query:  99   PLGGLQPRQGATAQELADPKQYQAFEERQTRELVQAYTSGVQQIPEIRARIEAAEQGGER  158
Sbjct:  44   SGGAIEPKGTRITLKVESEEDL-------NRDVVKSETATIS-IPELDLEIPPGPLGGFI  95

Query:  159  SAEEIDEARAALGQLEMMRDKLQRESPQLLPGDSAP  194
Sbjct:  96   TTVE--------GLLERVREVLSQAI-QEFRRDSDP  122


>gnl|CDD|21335 KOG3552, KOG3552, FERM domain protein FRM-8 [General function prediction only] Length = 1298 Score = 28.5 bits (63), Expect = 1.1 Identities = 37/207 (17%), Positives = 59/207 (28%), Gaps = 45/207 (21%)
Query:  32    ASSSSPPPARTVAALPAVDDRPPPLAPVRSQAVAPAYEAEPPAAELDREQAIQFMQMLR-  90
Sbjct:  936   DSRVSSISAIRFRIDPNNKE-NSGVVPAASSSASTPHCSNPGSSGPDTAQA-RPSQILPL  993

Query:  91    --------------DEGDPRSPPLGG--------------LQPRQGATAQELADPKQY--  120
Sbjct:  994   SQDLDGIAPKEPTIEHGDSSFSLSSGDPNPDRACLASNPGLNNVSQGDTLELQLEQLEMG  1053

Query:  121   -QAFEERQTRELVQAYTSGVQQIPEIRARIEAAEQGGERSAEEIDEARAALGQLEMMRDK  179
Sbjct:  1054  LESFCTNHIQETAPKYTEPLLSPRDEPRSDECGINPGEKIASIPT-KEEPQGQLSLERDR  1112

Query:  180   -------LQRESPQL---LPGDSAPTS  196
Sbjct:  1113  EVTNKNGFQEESRKDSGDSPGDVSNNV  1139


>gnl|CDD|18381 KOG0586, KOG0586, Serine/threonine protein kinase [General function prediction only] Length = 596 Score = 28.1 bits (62), Expect = 1.5 Identities = 23/157 (14%), Positives = 43/157 (27%), Gaps = 6/157 (3%)
Query:  28   ADMAASSSSPPPARTVAALPAVDDRPPPLAPVRSQAVAPAYEAEPPAAELDREQAIQFMQ  87
Sbjct:  428  LDEDRLPARDLPNASARSRSRATSPRIP--PFKWAYRLKDNKTNVG----DNIVENTLTR  481

Query:  88   MLRDEGDPRSPPLGGLQPRQGATAQELADPKQYQAFEERQTRELVQAYTSGVQQIPEIRA  147
Sbjct:  482  KNQSWSINERYVSPFVKTIADGSSFSCKKPAEPIKIGARQRQQGTDALAKIRDKMREIRK  541

Query:  148  RIEAAEQGGERSAEEIDEARAALGQLEMMRDKLQRES  184
Sbjct:  542  VLDANGCDYEQLRYHGDPGADAMVQWEMEVCKLPRLS  578


>gnl|CDD|19990 KOG2204, KOG2204, Mannosyl-oligosaccharide alpha-1,2-mannosidase and related glycosyl hydrolases [Carbohydrate transport and metabolism] Length = 625 Score = 28.0 bits (62), Expect = 1.8 Identities = 18/120 (15%), Positives = 33/120 (27%), Gaps = 3/120 (2%)
Query:  21   AGVLLAPADMAASSSSPPPARTVAALPAV--DDRPPPLAPVRSQAVAPAYEAEPPAAELD  78
Sbjct:  70   RIIFDFPAPPLAMEVIDAPDITTRVLEGLLSAYEEEMDRRLNKAKEVLPFILRSFDTPNR  129

Query:  79   REQAIQFMQM-LRDEGDPRSPPLGGLQPRQGATAQELADPKQYQAFEERQTRELVQAYTS  137
Sbjct:  130  IPIKKPFQKSDLRNRFPDRTVPSGQLTPALAFESLDPEDRNKYRERREKVKEMMKHAWNN  189


>gnl|CDD|7039 pfam03367, zf-ZPR1, ZPR1 zinc-finger domain. The zinc-finger protein ZPR1 is ubiquitous among eukaryotes. It is indeed known to be an essential protein in yeast. In quiescent cells, ZPR1 is localised to the cytoplasm. But in proliferating cells treated with EGF or with other mitogens, ZPR1 accumulates in the nucleolus. ZPR1 interacts with the cytoplasmic domain of the inactive EGF receptor (EGFR) and is thought to inhibit the basal protein tyrosine kinase activity of EGFR. This interaction is disrupted when cells are treated with EGF, though by themselves, inactive EGFRs are not sufficient to sequester ZPR1 to the cytoplasm. Upon stimulation by EGF, ZPR1 directly binds the eukaryotic translation elongation factor-1alpha (eEF-1alpha) to form ZPR1/eEF-1alpha complexes. These move into the nucleus, localising particularly at the nucleolus. Indeed, the interaction between ZPR1 and eEF-1alpha has been shown to be essential for normal cellular proliferation, and ZPR1 is thought to be involved in pre-ribosomal RNA expression. The alignment for this family shows a domain of which there are two copies in ZPR1 proteins. This family also includes several hypothetical archaeal proteins (from both Crenarchaeota and Euryarchaeota), which only contain one copy of the aligned region. This similarity between ZPR1 and archaeal proteins was not previously noted Length = 160 Score = 27.5 bits (61), Expect = 2.2 Identities = 17/63 (26%), Positives = 29/63 (46%), Gaps = 3/63 (4%)
Query:  124  EERQTRELVQAYTSGVQQIPEIRARIEAAEQGGERSAEE--IDEARAALGQLEMMRDKLQ  181
Sbjct:  63   EEDLNRDVVKSETASIS-IPELGLEIPGPALGGRFTTVEGLLERVIEGLSQDQEKRDSED  121

Query:  182  RES  184
Sbjct:  122  EET  124


>gnl|CDD|18046 KOG0250, KOG0250, DNA repair protein RAD18 (SMC family protein) [Replication, recombination and repair] Length = 1074 Score = 27.6 bits (61), Expect = 2.4 Identities = 13/46 (28%), Positives = 21/46 (45%)
Query:  125   ERQTRELVQAYTSGVQQIPEIRARIEAAEQGGERSAEEIDEARAAL  170
Sbjct:  301   QEKIEEKQGKIEEARQKLTEIEAKIGELKDEVDAQDEEIEEARKDL  346


Score = 25.6 bits (56), Expect = 9.4 Identities = 30/159 (18%), Positives = 54/159 (33%), Gaps = 12/159 (7%)
Query:  33    SSSSPPPARTVAALPA-VDDRPPPLAPVRSQAVAPAYEAEPPAAELDREQAIQFMQMLRD  91
Sbjct:  642   YSTRGTRARRPGVDEFSFDDEIEDLEREASRL-----QKEILELENQRREAEKNLEELEK  696

Query:  92    EGDPRSPPLGGLQPRQGATAQELADPKQYQAFEERQT---RELVQAYTSGVQQIPEIRAR  148
Sbjct:  697   KLRELSEHIEQIKRRIRKKRAEMTELKNTAEEKQVDISKLEDLAREIKKKEKEIEEKEAP  756

Query:  149   IEAAEQGGERSAEEIDEARAALGQLEMMRDKLQRESPQL  187
Sbjct:  757   LEKLKEELEHIELEAQELEE---YYAAGREKLQGEISKL  792


>gnl|CDD|21748 KOG3970, KOG3970, Predicted E3 ubiquitin ligase [Posttranslational modification, protein turnover, chaperones] Length = 299 Score = 26.6 bits (58), Expect = 4.8 Identities = 18/75 (24%), Positives = 24/75 (32%)
Query:  34   SSSPPPARTVAALPAVDDRPPPLAPVRSQAVAPAYEAEPPAAELDREQAIQFMQMLRDEG  93
Sbjct:  142  SPVPSPAPPQLKSAPVMHNEVPVHNNRSSTPATHLEMEDTASYSSPNHDVTFMRKKNPGP  201

Query:  94   DPRSPPLGGLQPRQG  108
Sbjct:  202  ESSADTRPLLQLRDG  216


>gnl|CDD|18775 KOG0982, KOG0982, Centrosomal protein Nuf [Cell cycle control, cell division, chromosome partitioning, Cytoskeleton] Length = 502 Score = 26.6 bits (58), Expect = 4.8 Identities = 20/111 (18%), Positives = 45/111 (40%), Gaps = 14/111 (12%)
Query:  76   ELDREQAIQFMQMLRDEGDPRSPPLGGLQPRQGATAQELADPKQYQAFEERQTRELVQAY  135
Sbjct:  264  EQRREQELRAEESLSEEER-RHREILIKKEREASLEKEN---------LQMRDQQLEEEN  313

Query:  136  TSGVQQIPEIRARIEAAEQGGERSAEEIDEARAALGQL----EMMRDKLQR  182
Sbjct:  314  TELRSLIARLKSLADKLAEEDQRSSDLLEALRLQLICEQKLRVRMNDILRR  364


>gnl|CDD|16998 pfam01576, Myosin_tail_1, Myosin tail. The myosin molecule is a multi-subunit complex made up of two heavy chains and four light chains it is a fundamental contractile protein found in all eukaryote cell types. This family consists of the coiled-coil myosin heavy chain tail region. The coiled-coil is composed of the tail from two molecules of myosin. These can then assemble into the macromolecular thick filament. The coiled-coil region provides the structural backbone the thick filament Length = 860 Score = 26.0 bits (57), Expect = 5.6 Identities = 18/66 (27%), Positives = 30/66 (45%), Gaps = 4/66 (6%)
Query:  118  KQYQAFEERQTRELVQAYTSGVQQIPEIRARIEAAEQGGERSAEEIDEARAALGQLEMMR  177
Sbjct:  577  KKYQQ----QVKELQTQVEEEQRAREDAREQLAVAERRATALEAELEELRSALEQAERAR  632

Query:  178  DKLQRE  183
Sbjct:  633  KQAETE  638


>gnl|CDD|27569 pfam07462, MSP1_C, Merozoite surface protein 1 (MSP1) C-terminus. This family represents the C-terminal region of merozoite surface protein 1 (MSP1) which are found in a number of Plasmodium species. MSP-1 is a 200-kDa protein expressed on the surface of the P. vivax merozoite. MSP-1 of Plasmodium species is synthesised as a high-molecular-weight precursor and then processed into several fragments. At the time of red cell invasion by the merozoite, only the 19-kDa C-terminal fragment (MSP-119), which contains two epidermal growth factor-like domains, remains on the surface. Antibodies against MSP-119 inhibit merozoite entry into red cells, and immunisation with MSP-119 protects monkeys from challenging infections. Hence, MSP-119 is considered a promising vaccine candidate Length = 574 Score = 26.2 bits (57), Expect = 5.7 Identities = 19/99 (19%), Positives = 28/99 (28%), Gaps = 25/99 (25%)
Query:  34   SSSPPPARTVAALPAVDDRPPPLAPVRSQAVAPAYEAEPPAAELDREQAIQFMQMLRDEG  93
Sbjct:  262  TTQEAKVTTVVTPPQADAAPSPLSVRPAGSSGSASGSTQ---------------------  300

Query:  94   DPRSPPLGGLQPRQGATAQELADPKQYQAFEERQTRELV  132
Sbjct:  301  ----IPTSGSVLGPGAAATELQQVVQLQNYDEEDDSLVV  335


>gnl|CDD|2058 pfam01519, DUF16, Protein of unknown function DUF16. The function of this protein is unknown. It appears to only occur in Mycoplasma pneumoniae Length = 96 Score = 26.1 bits (57), Expect = 5.7 Identities = 18/70 (25%), Positives = 35/70 (50%)
Query:  118  KQYQAFEERQTRELVQAYTSGVQQIPEIRARIEAAEQGGERSAEEIDEARAALGQLEMMR  177
Sbjct:  22   KQFDEFKDANNQRLTKIETKLDAQGEQIRKLTQTVEKQGEQIKELQVEQKAQGETLQLIL  81

Query:  178  DKLQRESPQL  187
Sbjct:  82   ETLQKINKRL  91


>gnl|CDD|21435 KOG3655, KOG3655, Drebrins and related actin binding proteins [Cytoskeleton] Length = 484 Score = 26.1 bits (57), Expect = 6.6 Identities = 28/109 (25%), Positives = 40/109 (36%), Gaps = 8/109 (7%)
Query:  36   SPPPARTVAALPAVDDRPPPLAPVRSQAVAPAYEAEPPAAELDREQAIQFMQMLRDEGDP  95
Sbjct:  360  PPPPSRETAEPSPVLDSEETRAASPQAW-APPYE-QPPAVQQPPEGPGSEEA---DLAFQ  414

Query:  96   RSPPLGGLQPRQGATAQELADPKQYQAFEERQTRELVQAYTSGVQQIPE  144
Sbjct:  415  QSAERAALAKVEPQTARALYD---YQAADDTEISFDPPDAITLIDQVDE  460


>gnl|CDD|26467 pfam06046, Sec6, Exocyst complex component Sec6. Sec6 is a component of the multiprotein exocyst complex. Sec6 interacts with Sec8, Sec10 and Exo70.These exocyst proteins localise to regions of active exocytosis-at the growing ends of interphase cells and in the medial region of cells undergoing cytokinesis-in an F-actin-dependent and exocytosis- independent manner Length = 725 Score = 25.7 bits (56), Expect = 7.9 Identities = 18/79 (22%), Positives = 31/79 (39%)
Query:  103  LQPRQGATAQELADPKQYQAFEERQTRELVQAYTSGVQQIPEIRARIEAAEQGGERSAEE  162
Sbjct:  204  REEREDQRAQAERDKKLTAFLPPTRPRNWKQKWLRALQESVEERFQRARGKQLEDRKEEK  263

Query:  163  IDEARAALGQLEMMRDKLQ  181
Sbjct:  264  SWLARLLLELGDFILEDLQ  282


>gnl|CDD|22417 KOG4643, KOG4643, Uncharacterized coiled-coil protein [Function unknown] Length = 1195 Score = 25.4 bits (55), Expect = 9.8 Identities = 19/83 (22%), Positives = 32/83 (38%), Gaps = 4/83 (4%)
Query:  106   RQGATAQELADPKQYQAFEERQTRELVQAYTSGVQQIPEIRARIEAAEQGGERSAEEIDE  165
Sbjct:  371   SDRALKLLLENRRLTGTLEELQSSS----YEELISKHLELEKEHKNLSKKHEILEERINQ  426

Query:  166   ARAALGQLEMMRDKLQRESPQLL  188
Sbjct:  427   LLQQLAELEDLEKKLQFELEKLL  449

Lambda     K      H
   0.312    0.129    0.366 

Gapped
Lambda     K      H
   0.267   0.0429    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 1100, Extension: 100
Number of Hits to DB: 3,427,237
Number of Sequences: 0
Number of extensions: 311274
Number of successful extensions: 435
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 400
length of query: 0
length of database: 5,506,404
effective HSP length: 89
effective length of query: -89
effective length of database: 3,900,933
effective search space: -347183037
effective search space used: 433003563
T: 11
A: 40
X1: 1600 (721.1 bits)
X2: 3800 (1463.8 bits)
X3: 6400 (2465.3 bits)
S1: 4200 (1895.8 bits)
S2: 59 (27.3 bits)