Score E
Sequences producing significant alignments: (bits) Value
gnl|CDD|27218 pfam07111, HCR, Alpha helical coiled-coil rod... 30 0.34
gnl|CDD|22637 smart00709, Zpr1, Duplicated domain in the ep... 29 1.1
gnl|CDD|21335 KOG3552, KOG3552, FERM domain protein FRM-8 [... 29 1.1
gnl|CDD|18381 KOG0586, KOG0586, Serine/threonine protein ki... 28 1.5
gnl|CDD|19990 KOG2204, KOG2204, Mannosyl-oligosaccharide al... 28 1.8
gnl|CDD|7039 pfam03367, zf-ZPR1, ZPR1 zinc-finger domain. T... 27 2.2
gnl|CDD|18046 KOG0250, KOG0250, DNA repair protein RAD18 (S... 28 2.4
gnl|CDD|21748 KOG3970, KOG3970, Predicted E3 ubiquitin liga... 27 4.8
gnl|CDD|18775 KOG0982, KOG0982, Centrosomal protein Nuf [Ce... 27 4.8
gnl|CDD|16998 pfam01576, Myosin_tail_1, Myosin tail. The my... 26 5.6
gnl|CDD|27569 pfam07462, MSP1_C, Merozoite surface protein ... 26 5.7
gnl|CDD|2058 pfam01519, DUF16, Protein of unknown function ... 26 5.7
gnl|CDD|21435 KOG3655, KOG3655, Drebrins and related actin ... 26 6.6
gnl|CDD|26467 pfam06046, Sec6, Exocyst complex component Se... 26 7.9
gnl|CDD|22417 KOG4643, KOG4643, Uncharacterized coiled-coil... 25 9.8
>gnl|CDD|27218 pfam07111, HCR, Alpha helical coiled-coil rod protein (HCR). This
family consists of several mammalian alpha helical
coiled-coil rod HCR proteins. The function of HCR is
unknown but it has been implicated in psoriasis in humans
and is thought to affect keratinocyte proliferation
Length = 760
Score = 30.2 bits (67), Expect = 0.34
Identities = 39/136 (28%), Positives = 59/136 (43%), Gaps = 12/136 (8%)
Query: 41 RTVAALPAVDDRPPPLAPVRSQAVAPAYEAEPPAAELDREQAIQFMQMLRDEGDPRSPPL 100
Sbjct: 443 RKVHTIKGLIARKLALAQLRQESCPPSEAAPPLDADLSLE-----LEQLREE---RNRLD 494
Query: 101 GGLQPRQGATAQELADPKQYQAFEERQTRELVQAYTSGVQQIPEIRA----RIEAAEQGG 156
Sbjct: 495 AELQLSAHLIQQEVGRAREQGEAERQQLIEVAQQLEQELQQAQESLASLGLQLEAARQGQ 554
Query: 157 ERSAEEIDEARAALGQ 172
Sbjct: 555 QESTEEAASLRQELTQ 570
>gnl|CDD|22637 smart00709, Zpr1, Duplicated domain in the epidermal growth factor-
and elongation factor-1alpha-binding protein Zpr1. Also
present in archaeal proteins
Length = 160
Score = 28.6 bits (64), Expect = 1.1
Identities = 21/96 (21%), Positives = 38/96 (39%), Gaps = 17/96 (17%)
Query: 99 PLGGLQPRQGATAQELADPKQYQAFEERQTRELVQAYTSGVQQIPEIRARIEAAEQGGER 158
Sbjct: 44 SGGAIEPKGTRITLKVESEEDL-------NRDVVKSETATIS-IPELDLEIPPGPLGGFI 95
Query: 159 SAEEIDEARAALGQLEMMRDKLQRESPQLLPGDSAP 194
Sbjct: 96 TTVE--------GLLERVREVLSQAI-QEFRRDSDP 122
>gnl|CDD|21335 KOG3552, KOG3552, FERM domain protein FRM-8 [General function
prediction only]
Length = 1298
Score = 28.5 bits (63), Expect = 1.1
Identities = 37/207 (17%), Positives = 59/207 (28%), Gaps = 45/207 (21%)
Query: 32 ASSSSPPPARTVAALPAVDDRPPPLAPVRSQAVAPAYEAEPPAAELDREQAIQFMQMLR- 90
Sbjct: 936 DSRVSSISAIRFRIDPNNKE-NSGVVPAASSSASTPHCSNPGSSGPDTAQA-RPSQILPL 993
Query: 91 --------------DEGDPRSPPLGG--------------LQPRQGATAQELADPKQY-- 120
Sbjct: 994 SQDLDGIAPKEPTIEHGDSSFSLSSGDPNPDRACLASNPGLNNVSQGDTLELQLEQLEMG 1053
Query: 121 -QAFEERQTRELVQAYTSGVQQIPEIRARIEAAEQGGERSAEEIDEARAALGQLEMMRDK 179
Sbjct: 1054 LESFCTNHIQETAPKYTEPLLSPRDEPRSDECGINPGEKIASIPT-KEEPQGQLSLERDR 1112
Query: 180 -------LQRESPQL---LPGDSAPTS 196
Sbjct: 1113 EVTNKNGFQEESRKDSGDSPGDVSNNV 1139
>gnl|CDD|18381 KOG0586, KOG0586, Serine/threonine protein kinase [General function
prediction only]
Length = 596
Score = 28.1 bits (62), Expect = 1.5
Identities = 23/157 (14%), Positives = 43/157 (27%), Gaps = 6/157 (3%)
Query: 28 ADMAASSSSPPPARTVAALPAVDDRPPPLAPVRSQAVAPAYEAEPPAAELDREQAIQFMQ 87
Sbjct: 428 LDEDRLPARDLPNASARSRSRATSPRIP--PFKWAYRLKDNKTNVG----DNIVENTLTR 481
Query: 88 MLRDEGDPRSPPLGGLQPRQGATAQELADPKQYQAFEERQTRELVQAYTSGVQQIPEIRA 147
Sbjct: 482 KNQSWSINERYVSPFVKTIADGSSFSCKKPAEPIKIGARQRQQGTDALAKIRDKMREIRK 541
Query: 148 RIEAAEQGGERSAEEIDEARAALGQLEMMRDKLQRES 184
Sbjct: 542 VLDANGCDYEQLRYHGDPGADAMVQWEMEVCKLPRLS 578
>gnl|CDD|19990 KOG2204, KOG2204, Mannosyl-oligosaccharide alpha-1,2-mannosidase and
related glycosyl hydrolases [Carbohydrate transport and
metabolism]
Length = 625
Score = 28.0 bits (62), Expect = 1.8
Identities = 18/120 (15%), Positives = 33/120 (27%), Gaps = 3/120 (2%)
Query: 21 AGVLLAPADMAASSSSPPPARTVAALPAV--DDRPPPLAPVRSQAVAPAYEAEPPAAELD 78
Sbjct: 70 RIIFDFPAPPLAMEVIDAPDITTRVLEGLLSAYEEEMDRRLNKAKEVLPFILRSFDTPNR 129
Query: 79 REQAIQFMQM-LRDEGDPRSPPLGGLQPRQGATAQELADPKQYQAFEERQTRELVQAYTS 137
Sbjct: 130 IPIKKPFQKSDLRNRFPDRTVPSGQLTPALAFESLDPEDRNKYRERREKVKEMMKHAWNN 189
>gnl|CDD|7039 pfam03367, zf-ZPR1, ZPR1 zinc-finger domain. The zinc-finger protein
ZPR1 is ubiquitous among eukaryotes. It is indeed known
to be an essential protein in yeast. In quiescent cells,
ZPR1 is localised to the cytoplasm. But in proliferating
cells treated with EGF or with other mitogens, ZPR1
accumulates in the nucleolus. ZPR1 interacts with the
cytoplasmic domain of the inactive EGF receptor (EGFR)
and is thought to inhibit the basal protein tyrosine
kinase activity of EGFR. This interaction is disrupted
when cells are treated with EGF, though by themselves,
inactive EGFRs are not sufficient to sequester ZPR1 to
the cytoplasm. Upon stimulation by EGF, ZPR1 directly
binds the eukaryotic translation elongation factor-1alpha
(eEF-1alpha) to form ZPR1/eEF-1alpha complexes. These
move into the nucleus, localising particularly at the
nucleolus. Indeed, the interaction between ZPR1 and
eEF-1alpha has been shown to be essential for normal
cellular proliferation, and ZPR1 is thought to be
involved in pre-ribosomal RNA expression. The alignment
for this family shows a domain of which there are two
copies in ZPR1 proteins. This family also includes
several hypothetical archaeal proteins (from both
Crenarchaeota and Euryarchaeota), which only contain one
copy of the aligned region. This similarity between ZPR1
and archaeal proteins was not previously noted
Length = 160
Score = 27.5 bits (61), Expect = 2.2
Identities = 17/63 (26%), Positives = 29/63 (46%), Gaps = 3/63 (4%)
Query: 124 EERQTRELVQAYTSGVQQIPEIRARIEAAEQGGERSAEE--IDEARAALGQLEMMRDKLQ 181
Sbjct: 63 EEDLNRDVVKSETASIS-IPELGLEIPGPALGGRFTTVEGLLERVIEGLSQDQEKRDSED 121
Query: 182 RES 184
Sbjct: 122 EET 124
>gnl|CDD|18046 KOG0250, KOG0250, DNA repair protein RAD18 (SMC family protein)
[Replication, recombination and repair]
Length = 1074
Score = 27.6 bits (61), Expect = 2.4
Identities = 13/46 (28%), Positives = 21/46 (45%)
Query: 125 ERQTRELVQAYTSGVQQIPEIRARIEAAEQGGERSAEEIDEARAAL 170
Sbjct: 301 QEKIEEKQGKIEEARQKLTEIEAKIGELKDEVDAQDEEIEEARKDL 346
Score = 25.6 bits (56), Expect = 9.4
Identities = 30/159 (18%), Positives = 54/159 (33%), Gaps = 12/159 (7%)
Query: 33 SSSSPPPARTVAALPA-VDDRPPPLAPVRSQAVAPAYEAEPPAAELDREQAIQFMQMLRD 91
Sbjct: 642 YSTRGTRARRPGVDEFSFDDEIEDLEREASRL-----QKEILELENQRREAEKNLEELEK 696
Query: 92 EGDPRSPPLGGLQPRQGATAQELADPKQYQAFEERQT---RELVQAYTSGVQQIPEIRAR 148
Sbjct: 697 KLRELSEHIEQIKRRIRKKRAEMTELKNTAEEKQVDISKLEDLAREIKKKEKEIEEKEAP 756
Query: 149 IEAAEQGGERSAEEIDEARAALGQLEMMRDKLQRESPQL 187
Sbjct: 757 LEKLKEELEHIELEAQELEE---YYAAGREKLQGEISKL 792
>gnl|CDD|21748 KOG3970, KOG3970, Predicted E3 ubiquitin ligase [Posttranslational
modification, protein turnover, chaperones]
Length = 299
Score = 26.6 bits (58), Expect = 4.8
Identities = 18/75 (24%), Positives = 24/75 (32%)
Query: 34 SSSPPPARTVAALPAVDDRPPPLAPVRSQAVAPAYEAEPPAAELDREQAIQFMQMLRDEG 93
Sbjct: 142 SPVPSPAPPQLKSAPVMHNEVPVHNNRSSTPATHLEMEDTASYSSPNHDVTFMRKKNPGP 201
Query: 94 DPRSPPLGGLQPRQG 108
Sbjct: 202 ESSADTRPLLQLRDG 216
>gnl|CDD|18775 KOG0982, KOG0982, Centrosomal protein Nuf [Cell cycle control, cell
division, chromosome partitioning, Cytoskeleton]
Length = 502
Score = 26.6 bits (58), Expect = 4.8
Identities = 20/111 (18%), Positives = 45/111 (40%), Gaps = 14/111 (12%)
Query: 76 ELDREQAIQFMQMLRDEGDPRSPPLGGLQPRQGATAQELADPKQYQAFEERQTRELVQAY 135
Sbjct: 264 EQRREQELRAEESLSEEER-RHREILIKKEREASLEKEN---------LQMRDQQLEEEN 313
Query: 136 TSGVQQIPEIRARIEAAEQGGERSAEEIDEARAALGQL----EMMRDKLQR 182
Sbjct: 314 TELRSLIARLKSLADKLAEEDQRSSDLLEALRLQLICEQKLRVRMNDILRR 364
>gnl|CDD|16998 pfam01576, Myosin_tail_1, Myosin tail. The myosin molecule is a
multi-subunit complex made up of two heavy chains and
four light chains it is a fundamental contractile protein
found in all eukaryote cell types. This family consists
of the coiled-coil myosin heavy chain tail region. The
coiled-coil is composed of the tail from two molecules of
myosin. These can then assemble into the macromolecular
thick filament. The coiled-coil region provides the
structural backbone the thick filament
Length = 860
Score = 26.0 bits (57), Expect = 5.6
Identities = 18/66 (27%), Positives = 30/66 (45%), Gaps = 4/66 (6%)
Query: 118 KQYQAFEERQTRELVQAYTSGVQQIPEIRARIEAAEQGGERSAEEIDEARAALGQLEMMR 177
Sbjct: 577 KKYQQ----QVKELQTQVEEEQRAREDAREQLAVAERRATALEAELEELRSALEQAERAR 632
Query: 178 DKLQRE 183
Sbjct: 633 KQAETE 638
>gnl|CDD|27569 pfam07462, MSP1_C, Merozoite surface protein 1 (MSP1) C-terminus.
This family represents the C-terminal region of merozoite
surface protein 1 (MSP1) which are found in a number of
Plasmodium species. MSP-1 is a 200-kDa protein expressed
on the surface of the P. vivax merozoite. MSP-1 of
Plasmodium species is synthesised as a
high-molecular-weight precursor and then processed into
several fragments. At the time of red cell invasion by
the merozoite, only the 19-kDa C-terminal fragment
(MSP-119), which contains two epidermal growth
factor-like domains, remains on the surface. Antibodies
against MSP-119 inhibit merozoite entry into red cells,
and immunisation with MSP-119 protects monkeys from
challenging infections. Hence, MSP-119 is considered a
promising vaccine candidate
Length = 574
Score = 26.2 bits (57), Expect = 5.7
Identities = 19/99 (19%), Positives = 28/99 (28%), Gaps = 25/99 (25%)
Query: 34 SSSPPPARTVAALPAVDDRPPPLAPVRSQAVAPAYEAEPPAAELDREQAIQFMQMLRDEG 93
Sbjct: 262 TTQEAKVTTVVTPPQADAAPSPLSVRPAGSSGSASGSTQ--------------------- 300
Query: 94 DPRSPPLGGLQPRQGATAQELADPKQYQAFEERQTRELV 132
Sbjct: 301 ----IPTSGSVLGPGAAATELQQVVQLQNYDEEDDSLVV 335
>gnl|CDD|2058 pfam01519, DUF16, Protein of unknown function DUF16. The function of
this protein is unknown. It appears to only occur in
Mycoplasma pneumoniae
Length = 96
Score = 26.1 bits (57), Expect = 5.7
Identities = 18/70 (25%), Positives = 35/70 (50%)
Query: 118 KQYQAFEERQTRELVQAYTSGVQQIPEIRARIEAAEQGGERSAEEIDEARAALGQLEMMR 177
Sbjct: 22 KQFDEFKDANNQRLTKIETKLDAQGEQIRKLTQTVEKQGEQIKELQVEQKAQGETLQLIL 81
Query: 178 DKLQRESPQL 187
Sbjct: 82 ETLQKINKRL 91
>gnl|CDD|21435 KOG3655, KOG3655, Drebrins and related actin binding proteins
[Cytoskeleton]
Length = 484
Score = 26.1 bits (57), Expect = 6.6
Identities = 28/109 (25%), Positives = 40/109 (36%), Gaps = 8/109 (7%)
Query: 36 SPPPARTVAALPAVDDRPPPLAPVRSQAVAPAYEAEPPAAELDREQAIQFMQMLRDEGDP 95
Sbjct: 360 PPPPSRETAEPSPVLDSEETRAASPQAW-APPYE-QPPAVQQPPEGPGSEEA---DLAFQ 414
Query: 96 RSPPLGGLQPRQGATAQELADPKQYQAFEERQTRELVQAYTSGVQQIPE 144
Sbjct: 415 QSAERAALAKVEPQTARALYD---YQAADDTEISFDPPDAITLIDQVDE 460
>gnl|CDD|26467 pfam06046, Sec6, Exocyst complex component Sec6. Sec6 is a component
of the multiprotein exocyst complex. Sec6 interacts with
Sec8, Sec10 and Exo70.These exocyst proteins localise to
regions of active exocytosis-at the growing ends of
interphase cells and in the medial region of cells
undergoing cytokinesis-in an F-actin-dependent and
exocytosis- independent manner
Length = 725
Score = 25.7 bits (56), Expect = 7.9
Identities = 18/79 (22%), Positives = 31/79 (39%)
Query: 103 LQPRQGATAQELADPKQYQAFEERQTRELVQAYTSGVQQIPEIRARIEAAEQGGERSAEE 162
Sbjct: 204 REEREDQRAQAERDKKLTAFLPPTRPRNWKQKWLRALQESVEERFQRARGKQLEDRKEEK 263
Query: 163 IDEARAALGQLEMMRDKLQ 181
Sbjct: 264 SWLARLLLELGDFILEDLQ 282
>gnl|CDD|22417 KOG4643, KOG4643, Uncharacterized coiled-coil protein [Function
unknown]
Length = 1195
Score = 25.4 bits (55), Expect = 9.8
Identities = 19/83 (22%), Positives = 32/83 (38%), Gaps = 4/83 (4%)
Query: 106 RQGATAQELADPKQYQAFEERQTRELVQAYTSGVQQIPEIRARIEAAEQGGERSAEEIDE 165
Sbjct: 371 SDRALKLLLENRRLTGTLEELQSSS----YEELISKHLELEKEHKNLSKKHEILEERINQ 426
Query: 166 ARAALGQLEMMRDKLQRESPQLL 188
Sbjct: 427 LLQQLAELEDLEKKLQFELEKLL 449
Lambda K H
0.312 0.129 0.366
Gapped
Lambda K H
0.267 0.0429 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 1100, Extension: 100
Number of Hits to DB: 3,427,237
Number of Sequences: 0
Number of extensions: 311274
Number of successful extensions: 435
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 400
length of query: 0
length of database: 5,506,404
effective HSP length: 89
effective length of query: -89
effective length of database: 3,900,933
effective search space: -347183037
effective search space used: 433003563
T: 11
A: 40
X1: 1600 (721.1 bits)
X2: 3800 (1463.8 bits)
X3: 6400 (2465.3 bits)
S1: 4200 (1895.8 bits)
S2: 59 (27.3 bits)